Parameter Value Reference
k1 0.01 Estimate
k2 0.002 Estimate
k3 0.001 Estimate
k4 0.3 Estimate
KM4 78 Estimate
V5 46.2 Estimate
KM5 117 Estimate
k6 0.05 Estimate
KM6 6170 Estimate
k7 5.5 Estimate
KM7 80.9 Estimate
k8 0.045 Estimate
k9 0.089 Estimate
k10 20 Estimate
KM10 80000 [BCC+00]
k11 0.037 Estimate
KM11 8800 Estimate
k12 20 Estimate
KM12 80000 [BCC+00]
k13 0.04 Estimate
KM13 48000 Estimate
k14 0.163 Estimate
KM14 48000 Estimate
k15 50 Estimate
KM15 20000 Estimate
V16 15000 Estimate
KM16 7260 Estimate
k17 0.09 Estimate
KM17 50 Estimate
k18 0.183 Estimate
KM18 500 Estimate
V19 78 Estimate
KM19 30 Estimate
k20 0.1 Estimate
KM20 13.2 Estimate
k21 5.6 Estimate
KM21 7200 Estimate
k22 0.04 Estimate
KM22 2600 Estimate
k23 0.45 Estimate
KM23 1250 Estimate
k24 5.17 Estimate
KM24 24500 Estimate
k25 0.05 Estimate
KM25 2150 Estimate
k26 0.4 Estimate
KM26 4316 Estimate
k27 0.089 Estimate
KM27 52000 Estimate
k28 30 Estimate
KM28 160 Estimate
k29 0.0308 Estimate
KM29 55000 Estimate
k30 32 Estimate
KM30 60 Estimate
k31 0.0017 Estimate
KM31 97.6 Estimate
k32 0.76 Estimate
KM32 181 Estimate
V33 468 Estimate
KM33 2.8 Estimate
k34 0.798 Estimate
KM34 10 Estimate
k35 0.04 Estimate
KM35 30000 Estimate
V36 821 Estimate
KM36 43300 Estimate
k37 0.397 Estimate
KM37 20700 Estimate
k38 0.04 Estimate
KM38 30000 Estimate
V39 821 Estimate
KM39 43300 Estimate
k40 0.0659 Estimate
KM40 42000 Estimate
k41 0.0148 Estimate
k42 0.0561 Estimate
k43 0.0624 Estimate
k44 0.002 [HCC+05]
k45 0.02 [HCC+05]
k46 0.00038 Estimate
k47 0.0155 Estimate
k48 0.14 Estimate
KM48 482 Estimate
V49 83000 Estimate
KM49 29100 Estimate
k50 0.0007 Estimate
k51 0.98 Estimate
Place Reference
Concentration
R 80 Estimate
PI3K 100 [MJ+04]
PIP2 7000 [CAZ04]
PTEN 0.1 Estimate
AKT 200 [BDF+05]
PDK1 1000 Estimate
PDK2 3 Estimate
PP2A 150 [KFK+03]
RAS 18900 [SEG+02]
RAF 66.4 [SEG+02]
MEK 36500 [SEG+02]
ERK 34900 [SEG+02]
MKP3 2.4 Estimate
P90RSK 5 [BF00]
BCL2 100 Estimate
BAD 100 Estimate
BAX 100 [JXD+98]
NOX5 2000 Estimate
ROS 200 Estimate
PAK 500 [LNL+04]
Note:
Assumed cell volume : 1 x 10^-12 L (from [SEG+02] where cell diameter is 15uM)
For Ras, Raf, MEK and ERK, the numbers given are in number of molecules
Ras 1.14 x 10^7 molecules
Raf 4 x 10^4 molecules
MEK 2.2 x 10^7 molecules
ERK 2.1 x 10^7 molecules
6.022 x
Avogadro constant:10^23
Thus, Ras = (1.14 x 10^7 / 6.022 x 10^23 ) / 1 x 10^-12 = 18900 nM
Same for Raf, MEK and ERK
References
[MJY+04]
[CAZ04]
[BDF+05]
[KFK+03]
[SEG+02]
[BF00]
[JXD+98]
[LNL+04]
[BCC+00]
[HCC+05]
References
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