Lectures :
Date Subject No. of Less. Teacher
1 8.2.2010 Characterization of procaryotic cells. 2 Prof. Kolář
Inner structures in bacteria.
2 15.2.2010 Bacterial metabolism and enzymes. 2 Doc. Hamal
3 22.2.2010 Surface structures in bacteria. Spores. 2 Prof. Kolář
4 1.3.2010 Growth and reproduction of bacteria. 2 Prof. Kolář
5 8.3.2010 Bacterial pathogenicity and virulence. 2 Prof. Kolář
6 15.3.2010 Exotoxins and endotoxins. 2 Prof. Kolář
7 22.3.2010 Microbial ecology. Microorganisms in healthy human body. 2 Prof. Kolář
8 29.3.2010 Bacterial genetics. Mutation. Gene transfer. Lysogenic conversion. Plasmids. Transposons. 2 Mgr. Sauer
9 5.4.2010 Easter Monday.
10 12.4.2010 Resistance of microbes to antibiotics (mechanisms and spreading). 2 Prof. Kolář
11 19.4.2010 Antimicrobial agents – classification. 2 Prof. Kolář
12 26.4.2010 Appropriate antibiotic therapy. 2 Prof. Kolář
13 3.5.2010 Sterilization and disinfection. 2 Doc. Hamal
14 10.5.2010 Antigens. Active immunization. 2 Doc. Hamal
Antibodies. Passive immunization.
15 17.5.2010 Endogenic infections. 2 Doc. Hamal
Nosocomial infections.
Exercises :
Date Subject No. of
Less.
1 11.2. - Dr.Vágnerová Principles of health protection and safety rules in the microbiology laboratory. The aims of the clinical microbiology 2
12.2. - Doc.Hamal laboratory. Principal microbiological procedures. Collection, handling and transport of clinical specimen.
2 18.2. - Dr.Vágnerová Microscopic techniques for diagnosis of infection. 2
19.2. - Doc.Hamal Native preparation. Bright field microscopy. Dark field microscopy. Motility of microbes and its observation.
3 25.2. - Dr.Vágnerová Microscopy with an immersion objective. Monochromatic staining. Gram´s staining. 2
26.2. - Prof.Kolář Form, size and arrangement of microbes.
4 4.3. - Dr.Vágnerová Staining of acid-fast microbes (according to Ziehl-Neelsen). 2
5.3- - Dr.Raclavský Laboratory diagnosis of tuberculosis. Laboratory diagnosis of diphtheria.
5 11.3. - Dr.Vágnerová Staining according to Giemsa. Microbial vaginal pictures. Laboratory diagnosis of genital tract infections. 2
12.3. - Doc.Hamal Staining of microbial capsules (according to Burri). Staining of microbial spores (according to Wirtz-Conklin).
6 18.3. - Dr.Vágnerová Cultivation of bacteria: 2
19.3. - Prof.Kolář Identification of microorganisms growing in aerobic culture - first part.
7 25.3. - Dr.Vágnerová Cultivation of bacteria: 2
26.3. - Prof.Kolář Identification of microorganisms growing in aerobic culture - second part.
8 1.4. - Dr.Vágnerová Cultivation of bacteria:Identification of microorganisms growing in anaerobic culture. 2
2.4. - Doc.Hejnar Video: Clostridium difficile - a problem of today.
9 8.4. - Dr.Vágnerová Determination of bacterial resistance to antibiotics. Disk susceptibility test, standard dilution micromethod – MIC 2
9.4. - Prof.Kolář method, E-test. Determination of antibiotic activity in combinations, assay for beta-lactamases (DDST).
10 15.4. - Dr.Vágnerová Drug monitoring, guidelines for antibiotic use. Determination of bacterial resistance to antibiotics – clinical 2
16.4. - Prof.Kolář outcome. Video: The misuse of a miracle.
11 22.4. - Mgr.Sauer Molecular-biology methods in medical microbiology. 2
23.4. - Mgr.Sauer
12 29.4. - Dr.Vágnerová Serology - first part: Agglutination and its modifications: Widal reaction, latexagglutination, haemagglutination, 2
30.4. - Doc.Hejnar slide agglutination. Precipitation and its modifications: precipitation in agar.
13 6.5. - Dr.Vágnerová Serology - second part: ELISA (enzyme-linked immunoassay). Princip of the neutralization test (ASLO). The 2
7.5. - Mgr.Sauer complement fixation test. Laboratory diagnosis of lues.
14 13.-14.5.2010 (náhrady I.) Differential microbiological diagnosis of most important bacterial species. 2
15 20.-21.5.2010 (náhrady II.) Non-cultural techniques for the laboratory diagnosis of infection. 2