Mouse Breeding Systems by HC111208235340

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									Mouse Breeding Systems
     General  Mouse
      Information
     Factors That Influence
      Breeding
     Breeding Systems
     Record Keeping
   General Mouse Information
 Average lifespan   is 2 years
 Breeding life is 8 months
 Reach sexual maturity at 28-42 days of age
 Breeding onset:
  Females 6-8 weeks Males 8-10 weeks
 Gestation is 19-21 days
 Litter size 2-16 pups per litter
 Breeding retirement:
 After having 5-6 litters or 6-8 months of age
     Factors That Influence
      Breeding Production
 Strain         Diet
 Age            Water
 Health         Nesting Material
 Technician     Number of mice
 Temperature    Age Bred
 Humidity       Disturbances
 Lighting
            Inbreeding
 Homozygous     animals
 Inbred strains are desirable due to
  their high degree of homogeneity
 A strain is considered to be inbred
  after 20 consecutive generations of
  brother/sister matings
         Line Breeding
 Homozygous    animals
 More intensive form of inbreeding
 Animals must have common
  ancestor in order to be bred to each
  other
             Outbreeding
 Heterozygous  animals
 Desirable due to their high degree of
  individual diversity
 Maintained by random matings. Usually a
  planned breeding scheme that ensures that
  only unrelated animals are bred
 Usually have bigger litters with stronger,
  larger pups than litters of inbred strains
Breeding Systems
Monogamous
Polygamous
Timed
Observed
Monogamous:
One male continuously bred to
one female.
   Pros:
 Ease of accurate record keeping
 Uses postpartum estrus
 Parents are known
 Don’t have to separate mice
 Little male burnout
 Exact date of birth for each litter
 Maximum number of litters per female during
  her life span
 Monogamous:
 One male continuously bred to one
 female
 Cons:
 Higher number     of males required
 Higher number of cages
 If one of the breeders in an inbred strain
  dies it can be difficult, if not impossible to
  replace
 If the male is aggressive he may
  cannibalize pups and possibly injure the
  female
Polygamous, AKA Harem Breeding:
One male bred to multiple females

 Pros:
 Requires fewer males
 Fewer number of cages needed
 Can reduce exposure of pups and females
  to male aggression
 Good way to get large number of litters
  quickly
Polygamous, AKA Harem Breeding:
One male bred to multiple females.
Cons:
 Higher level of technician responsibility
 Fewer litters per female
 Higher male burnout than monogamous
  breeding
 Can be labor intensive
 Accurate record keeping more difficult
 May not be sure who the mother is
 More than one litter can be born in a pan
 May loose postpartum estrus breeding
                Trios
 Often left together
 Co-parenting
 Can mix females
 Can help conception rates
 Watch for overcrowding
 Can be hard to determine maternal
  lineage of pups
          Timed Matings

 Mice  are bred for a specified period
 Predictable litter due date
 Stage of pregnancy
 Gestational age of pups
 Does not require checking for plugs
 Less labor intensive than observed mating
        Observed Matings
 Female   is bred to male. The next day
  begin checking for vaginal plugs
 Females are separated from the male once
  a plug is found
 Predictable litter due date
 Plug is not a guarantee of pregnancy
 Requires handling of mice
    DOCUMENTATION
 DOB
 Lineage
 Identification
 Strain
 Sex
 Dates   bred
 Weaning information
 Initials

								
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