FUNDAMENTALS OF IMMUNOLOGY
General Medicine
Sylabus of Lectures
1. Introduction to Immunology - history of immunology, immune system, immune
mechanisms, immune response
2. Cells of immune system
3. Lymphoid organs, mucosal immune system
4. Humoral nonspecific immune mechanism – complement system
5. Celullar nonspecific immune mechanism – phagocytosis
6. Main histocompatibility system, transplantation immunity
7. Antigens. Immunoglobulines – structure, genetics, classes
8. Immunoglobulines – izotypes, alotypes, monoclonal antibodies
9. Tolerance
10. Cytokines, adhesive molecules
11. Tumor immunity
12. Autoimmunity, immunodeficiency
13. Hypersensitivity reactions - I., II., III., IV. type
14. Immunomodulation – immunostimulation, immunorepression,
immunotherapy
Prof. Leonard Siegfried, M.D.,PhD.
The Head of Institute of Medical Microbiology
FUNDAMENTALS OF IMMUNOLOGY
General Medicine
Laboratory practice
1. Nonspecific humoral immunity – Complement system, lysozyme
- Methods for detection of lysozyme levels
- Complement assays – measurment of individual Complement
components ( C3, C4)
2. Nonspecific cellular immunity – phagocytosis, NK cells
- Evaluation of phagocytic index, phagocytic activity of
polymorphonuclear leucocytes (PMNL).
- Tests for intacellular killing
3. Specific humoral immunity – immunoglobulines
Detection of immunocomplexes
Detection of immuglobulines concentrations by radial immunodiffusion
4. Specific cellular immunity
- Separation of lymphocytes
5. Specific cellular immunity
- Flow cytometry – principle and evaluation of results
- Detection of T lymphocytes by E rosette-forming cells test
6. Autoimmunity, deficiences and transplantation
Detection of autoantibodies by immunofluorescence assay
Transplantation tests (detection of HLA antigenes)
7. Credit
Prof. Leonard Siegfried, M.D.,PhD.
The Head of Institute of Medical Microbiology
IMMUNOLOGY
Dental Medicine
Sylabus of Lectures
3. Introduction to Immunology - history of immunology, immune system, immune
mechanisms, immune response
4. Cells of immune system
3. Lymphoid organs, mucosal immune system
4. Humoral nonspecific immune mechanism – complement system
5. Celullar nonspecific immune mechanism – phagocytosis
6. Main histocompatibility system, transplantation immunity
7. Antigens. Immunoglobulines – structure, genetics, classes
14. Immunoglobulines – izotypes, alotypes, monoclonal antibodies
15. Tolerance
16. Cytokines, adhesive molecules
17. Tumor immunity
18. Autoimmunity, immunodeficiency
19. Hypersensitivity reactions - I., II., III., IV. type
14. Immunomodulation – immunostimulation, immunorepression,
immunotherapy
Prof. Leonard Siegfried, M.D.,PhD.
The Head of Institute of Medical Microbiology
IMMUNOLOGY
Dental Medicine
Laboratory practice
1. Nonspecific humoral immunity – Complement system, lysozyme
- Methods for detection of lysozyme levels
- Complement assays – measurment of individual Complement
components ( C3, C4)
2. Nonspecific cellular immunity – phagocytosis, NK cells
- Evaluation of phagocytic index, phagocytic activity of
polymorphonuclear leucocytes (PMNL).
- Tests for intacellular killing
3. Specific humoral immunity – immunoglobulines
- Detection of immunocomplexes
- Detection of immuglobulines concentrations by radial immunodiffusion
4. Specific cellular immunity
- Separation of lymphocytes
5. Specific cellular immunity
- Flow cytometry – principle and evaluation of results
- Detection of T lymphocytes by E rosette-forming cells test
6. Autoimmunity, deficiences and transplantation
- Detection of autoantibodies by immunofluorescence assay
- Transplantation tests (detection of HLA antigenes)
7. Credit
Prof. Leonard Siegfried, M.D.,PhD.
The Head of Institute of Medical Microbiology