Real World
Introduction:
Caffeine is the most widely taken drug in the world. Most people on any given day consume
caffeine and do not know how much they are getting. Labels are not required to display the
amount of caffeine and energy drinks often compare to the amount in coffee when that isn’t
always known. This lab is to determine the amount of caffeine per mass of coffee bean, per
volume of coffee, and per volume of energy drink.
Procedure:
Week One:
Extract caffeine from coffee beans of different manufacturers
Week Two:
1. Run extracted caffeine on HPLC and LCMS compared to known samples
2. Run Samples of coffee and energy drink on the HPLC and LCMS compared to known
samples and each other.
Method:
SFE parameters:
o 60°C oven, 60°C valve, 250 bar, no co-solvent for 1 hour
Standards for HPLC
o 1000 ppm, 500 ppm, 250 ppm, 100 ppm
Data:
Extractions
calculated
mass coffee before mass final mass difference ppm
dec 2.0783 75.074 75.14 0.066 275
8hr 2.0769 81.2475 81.2867 0.0392 163.3333
nescafe 2.0219 94.4531 94.5878 0.1347 561.25
foldgers 2.0693 78.0871 78.1276 0.0405 168.75
HPLC Data
w/o 10 w/o
concentration ppm 5ppm average
5hr 2209500 109.834 129.8711 113.5545 117.7532
dec 578989 28.30845 32.04044 32.02895 30.79261
nescaf 3406146 169.6663 201.6699 173.3868 181.5743
8hr 1958540 97.286 114.8135 101.0065 104.3687
foldgers 3680315 183.37475 218.12 187.0953 196.1967
extractions
dec
nescaf 8429 -0.21955 -2.19316 3.50095 0.362747
8hr
foldgers
Conclusion:
The SFE extraction portion of the real world did not yield any results. The reason for the lack of
results probably comes from the storage and collection of the extract. The extract was stored in
a beaker that was sealed for a week and allowing the extract to dry made it difficult to dissolve.
If we had collected directly into water it would have probably worked better. The HPLC portion
of the lab worked well and we determined that the commercial was correct. For some reason
the LC-MS did not give results that were able to be analyzed .