UNIVERSITY of WASHINGTON
PROTEOMICS RESOURCE
Chamber size
last updated 6/3/2011 Number of pressure cells
# part # Description
NA UWPR_PressureCellLid.ems
NA UWPR_PressureCellBody.ems
NA UWPR_PressureCellBody_smallChamber.ems
1 Y11N115H580 Regulator 1-Stage High Pressure 3000 PSI Brass, 0-2000 Del Range, CGA-580
2 SS-200-7-4 SS Swagelok Tube Fitting, Female Connector, 1/8 in. Tube OD x 1/4 in. Female NPT
3 AT3010 Alltech* Standard Stainless-Steel Tubing, 316,1/8X085,50FT
You'll also need a tubing cutter and a deburring tool, swagelok sell those but other vendors might be cheaper
4 SS-41GXS2 SS 1-Piece 40 Series 3-Way Ball Valve, 0.15 Cv, 1/8 in. Swagelok Tube Fitting
5 SS-2-TA-1-2 SS Swagelok Tube Fitting, Male Tube Adapter, 1/8 in. Tube OD x 1/8 in. Male NPT
6 SS-2-TA-7-4 SS Swagelok Tube Fitting, Female Tube Adapter, 1/8 in. Tube OD x 1/4 in. Female NPT
7 SS-4P-4M 316 Stainless Steel Purge Valve, 1/4 in. MNPT
8 SS-100-1-1 SS Swagelok Tube Fitting, Male Connector, 1/16 in. Tube OD x 1/16 in. Male NPT
9 NC9656755 Chromatography Research Supplies Ferrule 100% PTFE, Ferrule, 100% PTFE, 1/16 in. to 0.4mm, 10/PK, Upper Tempe
22-163741 Scienceware* Teflon Lab-Thread Tape
10 9464K64 O ring Viton, AS568A Dash Number 222, 1.5 id x 1.75 od x 1/8 width
11 92185A585 Type 316 Stainless STL Socket Head Cap Screw 5/16"-18 Thread, 1 1/4" Length
12 60475K74 Shaft Collars 1.25" OD x 0.75" ID x 0.5" length
13 2639T24 Sleeve Bearing, PTFE, 3/4" OD x 1/5" ID x 0.75" length
14 2639T25 Sleeve Bearing, PTFE, 3/4" OD x 1/5" ID x 1" length
15 1986K16 Compression Spring 0.5" OD x 2" long
Total
Tools:
5408A11 Double Open-End Steel Wrench 1/4" X 5/16" Size, 4-1/2" L, Chrome Finish
37095A38 Ball-Point T-Handle Hex Key 1/4" Hex, 10" Blade Length
7260A15 Adjustable Wrench with Cushion-Grip Chrome Finish, 12" L, 1-1/2" Jaw Cap
7260A16 Adjustable Wrench with Cushion-Grip Chrome Finish, 15" L, 1-11/16" Jaw Cap
you will also need various sized wrenches to assemble the pressure cell including 1/2, 7/16, /9/16, 11/16, 3/4; or get a co
small large
0 1
Qty Qty unit unit price total vendor
1 each $ 281.00 $ 281.00 emachineshop.com
0 1 each $ 385.70 $ 385.70 emachineshop.com
0 0 each $ - $ - emachineshop.com
1 each $ 396.00 $ 396.00 Airgas
1 each $ 10.97 $ 10.97 Swagelok
1 50ft $ 168.25 $ 168.25 Fisher Scientific
1 each $ 87.91 $ 87.91 Swagelok
1 each $ 6.26 $ 6.26 Swagelok
1 each $ 9.02 $ 9.02 Swagelok
1 each $ 21.53 $ 21.53 Swagelok
1 each $ 10.97 $ 10.97 Swagelok
0.1 pk of 10 $ 15.46 $ 1.55 Fisher Scientific
0.1 pack of 3 $ 24.53 $ 2.45 Fisher Scientific
1 25pk $ 11.45 $ 11.45 McMaster Carr
0.5 10pk $ 9.45 $ 4.73 McMaster Carr
0 2 each $ 3.58 $ 7.16 McMasterCarr
0 1 each $ 3.67 $ 3.67 McMasterCarr
0 1 each $ 3.92 $ 3.92 McMasterCarr
0 0 pk 6 $ 9.66 $ - McMasterCarr
$ 1,412.53
1 each $ 7.02 $ 7.02 McMasterCarr
1 each $ 29.63 $ 29.63 McMasterCarr
1 each $ 29.50 $ 29.50 McMasterCarr
1 each $ 51.79 $ 51.79 McMasterCarr
1/2, 7/16, /9/16, 11/16, 3/4; or get a couple of adjustable wrenches
comment
1
Note the price listed here is for one cell only, the more you order the cheaper they are (2 = $170.- ea, 3 = $140.- ea etc) 281
Note the price listed here is for one cell only, the more you order the cheaper they are (2 = $260.- ea, 3 = $210.- ea etc) 385.7
Note the price listed here is for one cell only, the more you order the cheaper they are (2 = $260.- ea, 3 = $210.- ea etc) 386.97
call for current price
to connect Regulator to 1/8" tubing
order as much as you need, brass would work too
3-Way Ball Valve, 2500 PSIG @ 100°F /172 @ BAR 37°C
to connect pressure cell to 3-Way Ball Valve
to connect Purge Valve to 3-Way Ball Valve
Purge Valve
to connect to the lid of the pressure cell to hold fused silica
you only need a little bit; For NPT fittings
for pressure cell body fits in groove on top of the body to create seal between lid and body
Bolts to attach lid
stand to hold Eppendorf tube inside the cell (large chamber only!)
stand to hold Eppendorf tube inside the cell (large chamber only!)
stand to hold Eppendorf tube inside the cell (large chamber only!)
stand to hold Eppendorf tube inside the cell (small chamber only!)
to tighten nut compressing teflon ferrule holding the fused silica
to tighten the bolts to secure the lid to the cell
adjustable wrench to attach regulator to gas cylinder, min jaw capacity 1 1/8"
longer version of the adjustable wrench, for more leverage, but more spendy…
2 3 4 5 6 7 8 9 10
170.7 138.89 120.69 115.65 107.7 102.74 98.65 93.58 88.26
252.8 205.83 178.73 180.33 167.1 158.79 154.42 147.96 142.08
256.38 209.76 182.43 183.79 170.74 164.45 158.16 151.85 145.62
UNIVERSITY of WASHINGTON
PROTEOMICS RESOURCE
last updated 6/3/2011
10 9464K64 O ring Viton, AS568A Dash Number 222, 1.5 id x 1.75 od x 1/8 width
AS568A Dash Number 222
Type O-Ring
O-Ring Type Standard
Cross Section Shape Round
Width 1/8" groove on pressure cell
Actual Width .139" depth 0.104
Inside Diameter 1-1/2"
Actual Inside Diameter 1.484" ID 1.464
Outside Diameter 1-3/4"
Actual Outside Diameter 1.762" OD 1.786
Material Viton® Fluoroelastomer
Viton® Fluoroelastomer Type Standard
Durometer Hard
Durometer Shore Shore A: 75
Temperature Range -15° to +400°F
Color Black
Specifications Met Not Rated
11 92185A585 Type 316 Stainless STL Socket Head Cap Screw 5/16"-18 Thread, 1 1/4" Length
Head Style Standard
Standard Head Style Standard
Material Type Stainless Steel
Finish Plain
Class Not Rated dimensions on pressure cell
Stainless Steel Type 316 Stainless Steel body lid
Drive Style Hex Socket
Inch Thread Size 5/16"-18 5/16"-18 NA
Length 1-1/4" 1" 0.5"
Thread Length Fully Threaded
Thread Direction Right Handed
Tip Type Plain
Self-Locking Method None
Screw Quantity Individual Screw
Hex Size 1/4"
Head Diameter 15/32"
Head Height 5/16"
Rockwell Hardness B70
Minimum Tensile Strength 70,000 psi
Thread Fit Class 3A
Specifications Met Not Rated
12 60475K74 Shaft Collars 1.25" OD x 0.75" ID x 0.5" length
Type Set Screw
System of Measurement Inch
Material Plastic
Plastic Material Nylon
Finish Plain
Color White
Bore Size 3/4"
Outside Diameter 1-1/4"
Width 9/16"
Screw Size 5/16"-18
Set/Cap Screw Material Type 304 Stainless Steel
Maximum Temperature +210° F
Specifications Met 3-A Sanitary Standards Compliant, Food and Drug Administration (FDA) Compliant, United States Department of Agricultur
Note Material is Nylon 101.
13 2639T24 Sleeve Bearing, PTFE, 3/4" OD x 1/5" ID x 0.75" length
Material Plastic
Plastic Type PTFE
Type Sleeve Bearings
1/2"
For Shaft Diameter (Inside Diameter)
Inside Diameter Tolerance +.000" to +.004"
Outside Diameter 3/4"
Outside Diameter Tolerance +.000" to +.004"
Length 3/4"
Length Tolerance ±.005"
Load (P Max) 500
Speed (V Max) 100
Load at Speed (PV Max) 1,000
Temperature Range -350° to +500° F
Specifications Met Not Rated
14 2639T25 Sleeve Bearing, PTFE, 3/4" OD x 1/5" ID x 1" length
Material Plastic
Plastic Type PTFE
Type Sleeve Bearings
1/2"
For Shaft Diameter (Inside Diameter)
Inside Diameter Tolerance +.000" to +.004"
Outside Diameter 3/4"
Outside Diameter Tolerance +.000" to +.004"
Length 1"
Length Tolerance ±.005"
Load (P Max) 500
Speed (V Max) 100
Load at Speed (PV Max) 1,000
Temperature Range -350° to +500° F
Specifications Met Not Rated
15 1986K16 Compression and Die Springs 0.5" OD x 2" long
Type Compression Springs
Material Stainless Steel
Stainless Steel Type Type 302 Stainless Steel
System of Measurement Inch
Outside Diameter 1/2"
Wire Size .047"
Overall Length 2"
Compressed Length 1.35"
Ends Closed
Wire Type Round Wire
Load 3.8 lbs.
Deflection at Load .65"
Rate 5.85 lbs./inch
Specifications Met Not Rated
ID 0.406
5 od x 1/8 width
6"-18 Thread, 1 1/4" Length
nited States Department of Agriculture (USDA) Compliant
92185A583 Type 316 Stainless STL Socket Head Cap Screw 5/16"-18 Thread, 1" Length
Head Style Standard
Standard
Standard Head Style
Stainless Steel
Material Type
Finish Plain
Class Not Rated
316 Stainless Steel
Stainless Steel Type
Drive Style Hex Socket
Size
Inch Thread5/16"-18
Length 1"
Fully
Thread Length Threaded
Right
Thread Direction Handed
Tip Type Plain
None
Self-Locking Method
Individual Screw
Screw Quantity
Hex Size 1/4"
15/32"
Head Diameter
5
Head Height /16"
B70
Rockwell Hardness
70,000 psi
Minimum Tensile Strength
Thread Fit Class 3A
Not Rated
Specifications Met
ead, 1" Length
UNIVERSITY of WASHINGTON
PROTEOMICS RESOURCE
last updated 6/3/2011
NextAdvance
Pressure cell overview http://www.nextadvance.com/product/capillary-loader-bomb-pressure-injection-cell
FRIT-KIT Kasil/formamide frit kit http://www.nextadvance.com/product/kasilformamide-frit-kit-267.cfm
Brechbuehler http://www.brechbuehler.com/Pressure-cell.878.0.html
NanoBaume http://www.westernfluids.net/Nanobaume.html
How to pack a capillary column http://www.benchfly.com/video.php?video=96
How to Bomb-load Capillary Tips or Frits for LC/MS http://www.benchfly.com/video.php?video=104
How to pull a capillary tip for LC/MS http://www.benchfly.com/video.php?video=102
omb-pressure-injection-cell-lc-ms-216.cfm
t-kit-267.cfm