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Table S10.- Downregulated genes in the ron1-1 microarray encoding chloroplast-targeted proteins



Fold change in

ron1-1

AGI code microarray

At4g08790 0.87

At2g37920 0.82

At5g14320 0.79

At4g02770 0.78

At5g08415 0.77

At4g26900 0.77

At1g62780 0.76

At5g48730 0.75

At1g23360 0.75

At2g43630 0.75

At3g24430 0.74

At1g75350 0.73

At5g19750 0.73

At5g27390 0.71

At3g28460 0.70

At1g59990 0.70

At2g39080 0.69

At4g17260 0.69

At2g42220 0.68

At2g17972 0.67

At1g67280 0.67

At1g32550 0.66

At5g64580 0.65

At3g60210 0.65

At5g08610 0.65

At3g52150 0.65

At1g03475 0.64

At1g10910 0.64

At5g16710 0.64

At4g30825 0.63

At5g05480 0.63

At3g03710 0.63

At5g07900 0.63

At2g24060 0.62

At2g48120 0.62

At1g70070 0.62

At3g26900 0.61

At4g33350 0.61

At3g54090 0.60

At5g51100 0.59

At3g04260 0.59

At4g24750 0.59

At5g58250 0.59

At3g24506 0.58

At2g44760 0.58

At3g52155 0.58

At2g24120 0.58

At3g24590 0.57

At5g04900 0.56

At4g20120 0.56

At5g66090 0.56

At2g34640 0.55

At1g31410 0.55

At1g74640 0.54

At3g20930 0.53

At2g04530 0.53

At1g31160 0.53

At3g17170 0.51

At5g66470 0.51

At1g51110 0.51

At4g10750 0.51

At5g10160 0.50

At1g10522 0.49

At3g20440 0.49

At3g59980 0.49

At5g24490 0.49

At5g04360 0.48

At5g64816 0.48

At1g22590 0.47

At3g05800 0.47

At3g14900 0.47

At2g02740 0.46

At2g31840 0.46

At3g09580 0.45

At3g28760 0.45

At1g70820 0.45

At5g10390 0.44

At5g58260 0.43

At2g36390 0.43

At5g26570 0.42

At1g67660 0.42

At1g54570 0.41

At3g04550 0.41

At1g24610 0.40

At5g64860 0.38

At3g56330 0.37

At1g10760 0.36

At1g05385 0.36

At1g66520 0.35

At2g47840 0.34

At2g30930 0.33

At5g15530 0.30

At3g26740 0.29

At1g04620 0.27

At5g06690 0.14

At4g04330 0.11





Bold highlighted genes contain the "evening element" in their promoter.

Downregulated genes in the ron1-1 microarray encoding chloroplast-targeted proteins







Description

nitrilase, putative

EMB1513__EMB1513 (EMBRYO DEFECTIVE 1513); copper ion transmembrane transporter

30S ribosomal protein S13, chloroplast (CS13)

PSAD-1__PSAD-1 (photosystem I subunit D-1)

lipoic acid synthase family protein

AT-HF_HISHF_HISN4__AT-HF (ARABIDOPSIS THALIANA HISF PROTEIN)

similar to hypothetical protein [Vitis vinifera] (GB:CAN83165.1)

pentatricopeptide (PPR) repeat-containing protein

UbiE/COQ5 methyltransferase family protein

similar to glycine-rich protein [Arabidopsis thaliana] (TAIR:AT3G59640.2); similar to glycine-rich protein [Arabidopsis

HCF101__HCF101 (HIGH-CHLOROPHYLL-FLUORESCENCE 101); ATP binding

EMB2184__EMB2184 (EMBRYO DEFECTIVE 2184); structural constituent of ribosome

peroxisomal membrane 22 kDa family protein

expressed protein

similar to hypothetical protein [Vitis vinifera] (GB:CAN74970.1); contains InterPro domain Protein of unknown function

DEAD/DEAH box helicase, putative (RH22)

binding / catalytic

L-lactate dehydrogenase, putative

rhodanese-like domain-containing protein

similar to unnamed protein product [Vitis vinifera] (GB:CAO48225.1)

lactoylglutathione lyase, putative / glyoxalase I, putative

ferredoxin family protein

AAA-type ATPase family protein

chloroplast chaperonin 10, putative

DEAD box RNA helicase (RH26)

RNA recognition motif (RRM)-containing protein

ATCPO-I_HEMF1_LIN2__LIN2 (LESION INITIATION 2); coproporphyrinogen oxidase

similar to PTAC2 (PLASTID TRANSCRIPTIONALLY ACTIVE2) [Arabidopsis thaliana] (TAIR:AT1G74850.1); similar

DHAR3__DHAR3 (DEHYDROASCORBATE REDUCTASE 1); glutathione dehydrogenase (ascorbate)

pentatricopeptide (PPR) repeat-containing protein

similar to unknown protein [Arabidopsis thaliana] (TAIR:AT3G14920.1); similar to hypothetical protein [Vitis vinifera]

RIF10__RIF10 (RESISTANT TO INHIBITION WITH FSM 10); 3'-5'-exoribonuclease/ RNA binding / nucleic acid

mitochondrial transcription termination factor family protein / mTERF family protein

translation initiation factor 3 (IF-3) family protein

PAC__PAC (PALE CRESS)

PDE317_EMB25_ISE2__EMB25 (EMBRYO DEFECTIVE 25); ATP-dependent helicase/ RNA helicase

shikimate kinase family protein

chloroplast inner membrane import protein Tic22, putative

pfkB-type carbohydrate kinase family protein

FSD2__FSD2 (FE SUPEROXIDE DISMUTASE 2); iron superoxide dismutase

PTAC3__PTAC3 (PLASTID TRANSCRIPTIONALLY ACTIVE3); DNA binding

similar to rhodanese-like domain-containing protein [Arabidopsis thaliana] (TAIR:AT3G08920.1); similar to unnamed

similar to hypothetical protein [Vitis vinifera] (GB:CAN75840.1); contains domain PD020337 (PD020337)

similar to unknown protein [Arabidopsis thaliana] (TAIR:AT2G17240.1); similar to unknown [Populus trichocarpa]

similar to unnamed protein product [Vitis vinifera] (GB:CAO70515.1)

similar to hypothetical protein OsJ_027559 [Oryza sativa (japonica cultivar-group)] (GB:EAZ44076.1); contains

PDE319_SCA3__PDE319/SCA3 (SCABRA 3); DNA binding / DNA-directed RNA polymerase

signal peptidase I family protein

short-chain dehydrogenase/reductase (SDR) family protein

ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase-related

similar to unnamed protein product [Vitis vinifera] (GB:CAO23263.1); contains domain Adenine nucleotide alpha

PTAC12__PTAC12 (PLASTID TRANSCRIPTIONALLY ACTIVE12)

putrescine-binding periplasmic protein-related

similar to unnamed protein product [Vitis vinifera] (GB:CAO64553.1); contains domain SSF53474 (SSF53474);

RNA recognition motif (RRM)-containing protein

CPZ__CPZ

zinc-binding protein, putative / protein kinase C inhibitor, putative

RFC3__RFC3 (REGULATOR OF FATTY-ACID COMPOSITION 3); structural constituent of ribosome

GTP binding / RNA binding

plastid-lipid associated protein PAP / fibrillin family protein

HpcH/HpaI aldolase family protein

beta-hydroxyacyl-ACP dehydratase, putative

similar to unnamed protein product [Vitis vinifera] (GB:CAO40945.1)

BE1_EMB2729__BE1/EMB2729 (BRANCHING ENZYME 1); alpha-amylase

tRNA-binding region domain-containing protein

30S ribosomal protein, putative

ATPU1__ATLDA/ATPU1 (PULLULANASE 1); alpha-amylase/ limit dextrinase

Identical to Uncharacterized protein At5g64816 precursor [Arabidopsis Thaliana] (GB:Q8L8Q8;GB:Q9LV96); similar

AGL87__AGL87

transcription factor

similar to unnamed protein product [Vitis vinifera] (GB:CAO39402.1); contains domain PTHR12839 (PTHR12839)

ATWHY3_PTAC11__ATWHY3/PTAC11 (A. THALIANA WHIRLY 3)

similar to unknown protein [Arabidopsis thaliana] (TAIR:AT4G28590.1); similar to hypothetical protein [Vitis vinifera]

amine oxidase family protein

similar to unnamed protein product [Vitis vinifera] (GB:CAO14940.1); contains InterPro domain 3-dehydroquinate

phosphoglucomutase, putative / glucose phosphomutase, putative

histone H3

Encodes subunit NDH-N of NAD(P)H:plastoquinone dehydrogenase complex (Ndh complex) present in the thylakoid

BE3_SBE2.1__SBE2.1 (STARCH BRANCHING ENZYME 2.1); 1,4-alpha-glucan branching enzyme

ATGWD3_PWD_ATGWD3_OK1__PWD (PHOSPHOGLUCAN WATER DIKINASE)

DNA binding / magnesium ion binding / nuclease

esterase/lipase/thioesterase family protein

similar to unknown protein [Arabidopsis thaliana] (TAIR:AT5G28500.1); similar to unnamed protein product [Vitis

SET domain-containing protein

DPE1__DPE1 (DISPROPORTIONATING ENZYME); 4-alpha-glucanotransferase

N2,N2-dimethylguanosine tRNA methyltransferase family protein

SEX1_GWD_SOP__SEX1 (STARCH EXCESS 1)

photosystem II 11 kDa protein-related

PDE194__PDE194 (PIGMENT DEFECTIVE 194); formyltetrahydrofolate deformylase/ hydroxymethyl-, formyl- and

tic20 protein-related

similar to unknown protein [Arabidopsis thaliana] (TAIR:AT1G06540.1)

BCCP2_CAC1-B_BCCP2__BCCP2 (biotin carboxyl carrier protein 2); biotin binding

CCL__CCL (CCR-LIKE)

coenzyme F420 hydrogenase family / dehydrogenase, beta subunit family

(THIOREDOXIN-LIKE 5); thiol-disulfide exchange intermediate

similar to unnamed protein product [Vitis vinifera] (GB:CAO69665.1)





hted genes contain the "evening element" in their promoter.

255118_at

266091_at

250190_at

255457_at

246005_at

253921_at

262693_at









248669_at









263044_at









260586_at









257168_at









261119_at









245937_at









257903_at

263679_at



266192_at 266193_at







245324_at









267635_at









265819_at









264970_at



256468_at







247235_at







251425_at







250529_at









252032_at









264820_at

260465_at









246454_at









253589_at









250784_at

259344_at









250554_at









266575_at



262316_at









264748_at







258281_at









253381_at









251935_at









248493_at









258573_at

254117_at









247862_at









258135_at









266874_at









252039_at

265991_at







258128_at









250812_at









247139_at









266957_at









256483_at









260232_at









256805_at

263864_at









263701_at

257891_at



247098_at









245744_at 245745_at







254937_at









250470_at









263209_at









257667_at









251442_at







249742_at









245712_at









247258_at



261942_at



258742_at

257207_at









267474_at









263488_at



258708_at









256585_at









262309_at

250434_at

247816_at









263912_at









246829_at









245188_at







264186_at









258800_at







265021_at









247216_at









251730_at

262784_at







261388_at









256361_at



266463_at









267209_at









246565_at



257832_at









264597_at









250649_at









255331_at

nitrilase,

putative

EMB1513__

EMB1513

30S

ribosomal

PSAD-

1__PSAD-1

lipoic acid

synthase

AT-

HF_HISHF_

similar to

hypothetical

protein [Vitis

vinifera]

(GB:CAN83165.

1)



pentatricopeptide

(PPR) repeat-

containing

protein



UbiE/COQ5

methyltransferas

e family protein







similar to glycine-

rich protein

[Arabidopsis

thaliana]

(TAIR:AT3G596

40.2); similar to

glycine-rich

protein

[Arabidopsis

thaliana]

(TAIR:AT3G596

40.1); similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO46269.

1)





HCF101__HCF1

01 (HIGH-

CHLOROPHYLL-

FLUORESCENC

E 101); ATP

binding







EMB2184__EMB

2184 (EMBRYO

DEFECTIVE

2184); structural

constituent of

ribosome

peroxisomal

membrane 22

kDa family

protein







similar to

hypothetical

protein [Vitis

vinifera]

(GB:CAN74970.

1); contains

InterPro domain

Protein of

unknown

function

methylase

putative

(InterPro:IPR016

065)

DEAD/DEAH

box helicase,

putative (RH22)



binding / catalytic

L-lactate

dehydrogenase,

putative

rhodanese-like

domain-

containing

protein

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO48225.

1)





lactoylglutathion

e lyase, putative

/ glyoxalase I,

putative

ferredoxin family

protein

AAA-type

ATPase family

protein

chloroplast

chaperonin 10,

putative



DEAD box RNA

helicase (RH26)





RNA recognition

motif (RRM)-

containing

protein





ATCPO-

I_HEMF1_LIN2_

_LIN2 (LESION

INITIATION 2);

coproporphyrino

gen oxidase

similar to PTAC2

(PLASTID

TRANSCRIPTIO

NALLY

ACTIVE2)

[Arabidopsis

thaliana]

(TAIR:AT1G748

50.1); similar to

hypothetical

protein

OsI_002384

[Oryza sativa

(indica cultivar-

group)]

(GB:EAY74537.1

); similar to

putative PPR

protein [Oryza

sativa (japonica

cultivar-group)]

(GB:BAD87953.

1); similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO24702.

1); contains

InterPro domain

Pentatricopeptid

e repeat

(InterPro:IPR002

885)



DHAR3__DHAR

3

(DEHYDROASC

ORBATE

REDUCTASE 1);

glutathione

dehydrogenase

(ascorbate)





pentatricopeptide

(PPR) repeat-

containing

protein





similar to

unknown protein

[Arabidopsis

thaliana]

(TAIR:AT3G149

20.1); similar to

hypothetical

protein [Vitis

vinifera]

(GB:CAN73340.

1)

RIF10__RIF10

(RESISTANT TO

INHIBITION

WITH FSM 10);

3'-5'-

exoribonuclease/

RNA binding /

nucleic acid

binding





mitochondrial

transcription

termination

factor family

protein / mTERF

family protein

translation

initiation factor 3

(IF-3) family

protein

PAC__PAC

(PALE CRESS)





PDE317_EMB25

_ISE2__EMB25

(EMBRYO

DEFECTIVE 25);

ATP-dependent

helicase/ RNA

helicase



shikimate kinase

family protein



chloroplast inner

membrane

import protein

Tic22, putative

pfkB-type

carbohydrate

kinase family

protein





FSD2__FSD2

(FE

SUPEROXIDE

DISMUTASE 2);

iron superoxide

dismutase

PTAC3__PTAC3

(PLASTID

TRANSCRIPTIO

NALLY

ACTIVE3); DNA

binding

similar to

rhodanese-like

domain-

containing

protein

[Arabidopsis

thaliana]

(TAIR:AT3G089

20.1); similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO64218.

1); contains

InterPro domain

Rhodanese-like

(InterPro:IPR001

763)



similar to

hypothetical

protein [Vitis

vinifera]

(GB:CAN75840.

1); contains

domain

PD020337

(PD020337)





similar to

unknown protein

[Arabidopsis

thaliana]

(TAIR:AT2G172

40.1); similar to

unknown

[Populus

trichocarpa]

(GB:ABK93895.1

)

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO70515.

1)









similar to

hypothetical

protein

OsJ_027559

[Oryza sativa

(japonica cultivar-

group)]

(GB:EAZ44076.1

); contains

InterPro domain

Phosphoglycerat

e mutase

(InterPro:IPR013

078)

PDE319_SCA3_

_PDE319/SCA3

(SCABRA 3);

DNA binding /

DNA-directed

RNA polymerase



signal peptidase

I family protein



short-chain

dehydrogenase/r

eductase (SDR)

family protein









similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO23263.

1); contains

domain Adenine

nucleotide alpha

hydrolases-like

(SSF52402)

PTAC12__PTAC

12 (PLASTID

TRANSCRIPTIO

NALLY

ACTIVE12)

putrescine-

binding

periplasmic

protein-related





similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO64553.

1); contains

domain

SSF53474

(SSF53474);

contains domain

G3DSA:3.40.50.

1820

(G3DSA:3.40.50.

1820)





RNA recognition

motif (RRM)-

containing

protein

CPZ__CPZ







zinc-binding

protein, putative /

protein kinase C

inhibitor, putative

RFC3__RFC3

(REGULATOR

OF FATTY-ACID

COMPOSITION

3); structural

constituent of

ribosome

GTP binding /

RNA binding

plastid-lipid

associated

protein PAP /

fibrillin family

protein

HpcH/HpaI

aldolase family

protein





beta-hydroxyacyl-

ACP

dehydratase,

putative

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO40945.

1)

BE1_EMB2729_

_BE1/EMB2729

(BRANCHING

ENZYME 1);

alpha-amylase

tRNA-binding

region domain-

containing

protein



30S ribosomal

protein, putative

ATPU1__ATLDA

/ATPU1

(PULLULANASE

1); alpha-

amylase/ limit

dextrinase



Identical to

Uncharacterized

protein

At5g64816

precursor

[Arabidopsis

Thaliana]

(GB:Q8L8Q8;GB

:Q9LV96);

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO22147.

1)



AGL87__AGL87

transcription

factor

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO39402.

1); contains

domain

PTHR12839

(PTHR12839)

ATWHY3_PTAC

11__ATWHY3/P

TAC11 (A.

THALIANA

WHIRLY 3)









similar to

unknown protein

[Arabidopsis

thaliana]

(TAIR:AT4G285

90.1); similar to

hypothetical

protein [Vitis

vinifera]

(GB:CAN75809.

1); contains

InterPro domain

Thioredoxin-like

fold

(InterPro:IPR012

336)

amine oxidase

family protein





similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO14940.

1); contains

InterPro domain

3-

dehydroquinate

synthase,

prokaryotic-type

(InterPro:IPR002

812)





phosphoglucomu

tase, putative /

glucose

phosphomutase,

putative

histone H3

Encodes subunit

NDH-N of

NAD(P)H:plastoq

uinone

dehydrogenase

complex (Ndh

complex) present

in the thylakoid

membrane of

chloroplasts.

This subunit is

thought to be

required for Ndh

complex

assembly.







BE3_SBE2.1__S

BE2.1 (STARCH

BRANCHING

ENZYME 2.1);

1,4-alpha-glucan

branching

enzyme

ATGWD3_PWD

_ATGWD3_OK1

__PWD

(PHOSPHOGLU

CAN WATER

DIKINASE)

DNA binding /

magnesium ion

binding /

nuclease

esterase/lipase/t

hioesterase

family protein





similar to

unknown protein

[Arabidopsis

thaliana]

(TAIR:AT5G285

00.1); similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO65032.

1)

SET domain-

containing

protein

DPE1__DPE1

(DISPROPORTI

ONATING

ENZYME); 4-

alpha-

glucanotransfera

se





N2,N2-

dimethylguanosi

ne tRNA

methyltransferas

e family protein

SEX1_GWD_SO

P__SEX1

(STARCH

EXCESS 1)

photosystem II

11 kDa protein-

related



PDE194__PDE1

94 (PIGMENT

DEFECTIVE

194);

formyltetrahydrof

olate

deformylase/

hydroxymethyl-,

formyl- and

related

transferase

tic20 protein-

related





similar to

unknown protein

[Arabidopsis

thaliana]

(TAIR:AT1G065

40.1)





BCCP2_CAC1-

B_BCCP2__BC

CP2 (biotin

carboxyl carrier

protein 2); biotin

binding

CCL__CCL

(CCR-LIKE)





coenzyme F420

hydrogenase

family /

dehydrogenase,

beta subunit

family

(THIOREDOXIN-

LIKE 5); thiol-

disulfide

exchange

intermediate

similar to

unnamed protein

product [Vitis

vinifera]

(GB:CAO69665.

1)



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