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CASE 8



DIAGNOSIS: OSSIFYING FIBROMA, JUVENILE PSAMMOMATOID

VARIANT



CLINICAL HISTORY:

A 14 year old girl with a history of chronic headaches presented with an acute

exacerbation of a right sided headache with associated sinusitis. Associated symptoms

included photophobia. A sinus CT scan identified a 1 cm osseous lesion located at the

right frontoethmoidal sinus complex. The patient subsequently underwent endoscopic

stereotactic-guided sinus surgery with complete removal of the tumor.



DISCUSSION:

Benign fibro-osseous lesions (BFOLs) of the craniofacial bones comprise a diverse group

of clinicopathologic entities and include developmental, neoplastic and reactive

processes. The recognized BFOLs are fibrous dysplasia, ossifying fibroma (and its

variants) as well as the osseous (cemento-osseous) dysplasias. All BFOLs are

characterized by the replacement of normal bone by fibrous connective tissue with

varying degrees of mineralized components including osteoid, bone and cementum-like

calcifications. As such, the histologic diagnosis is relatively straightforward; the main

challenge lies in the subclassification of these entities. In addition, other processes may

mimic BFOLs such as chronic osteomyelitis, central giant cell lesion, renal

osteodystrophy, osteoblastoma and osteosarcoma.



Clinically, BFOLs may present with pain and expansion of the jawbones or may be

asymptomatic and found incidentally on imaging studies. For most of the subtypes, there

are rather distinct clinical and radiologic findings. Because of the significant histologic

overlap among the BFOLs, the diagnosis rests on the constellation of clinical, radiologic,

operative and histologic findings. If these are not available and one is dealing with a

BFOL, the pathologist should diagnose the lesion as “benign fibro-osseous lesion” with a

comment.



Ossifying Fibroma

Ossifying fibroma (OF) is considered a true neoplasm of the jaws and, less commonly,

other craniofacial bones. OF was previously thought to originate from cells of the

periodontal ligament, but, this does not explain tumors arising in non-tooth bearing

bones. There is a slight female predilection and most occur in the third and fourth

decades. Conventional OF more frequently arises in the mandible (70%) with a

predilection for the posterior elements in both jaw bones. They are usually slow growing

tumors that expand the involved bone and average about 3-4 cm. Others can be quite

large resulting in marked facial distortion.



Radiologically, OF appear as unilocular lesions with sharply demarcated and smooth

borders. Early lesions are radiolucent and, depending on the amount of calcification,

older lesions may have a mixed radiolucent and radiopaque appearance. Because OF are

sharply demarcated from the native bone, the surgeon typically is able to shell the lesion

out easily.



Histologically, OF are demarcated from the uninvolved native bone lacking evidence of

fusion with the cortex. OF usually have a variegated microscopic appearance with

varying amounts and quality of connective and mineralized tissues. The stroma is

moderately cellular and comprised of bland spindle-oval fibroblastic cells. The cells can

be haphazardly arranged or grow in short fascicles and even storiform patterns. The

mineralized tissues can be trabeculae of woven bone, trabeculae or larger zones of

lamellar bone, and/or round/ovoid calcium deposits resembling cementum (hence the old

term “cemento-ossifying fibroma”). These calcified spherules often coalesce to form

nodules. Most OF consist of a haphazard mixture of these mineralized elements.

Osteoblastic rimming can be seen in any of these mineralized deposits.



Most lesions can be surgically excised completely with curettage or enucleation.

Recurrence is rare with conventional OF.



Juvenile Ossifying Fibroma

A subset of OF have distinct clinical and histologic findings and have been termed

juvenile OF (JOF). There are two recognized subtypes, the psammomatoid and

trabecular variants. Psammomatoid juvenile OF (PJOF) is more common than trabecular

juvenile OF (TJOF). Both can be seen in patients of any age, however, most arise in

patients in the first and second decades. PJOF tends to occur in slightly older individuals

(mean 20 yrs) than TJOF (mean 9-12 yrs). There appears to be no sex predilection for

JOF. PJOF most often arises in the orbit, paranasal sinuses and calvaria (75%) with the

remaining 25% occurring in the maxilla and mandible. In contrast, TJOF usually

develops in the jawbones (95%) with a predilection for the maxilla. Imaging findings are

less specific but most are mixed radiolucent and radiopaque and are well-demarcated.



Histologically, PJOF consists of a cell rich, paucivascular stroma with little collagen.

The cellularity tends to be uniform and the matrix also tends to be of uniform

composition and distribution. The mineralized elements consist of basophilic, acellular

or paucicellular, round/oval structures imparting a superficial resemblance to psammoma

bodies. TJOF consist of a variably cellular stroma with plump spindle cells associated

with varying amounts of collagen. In the cellular areas, condensation of plump

osteoblasts associated with thin seams of osteoid can be identified. These appear to

mature to progressively larger trabeculae of woven bone lined by osteoblasts. Occasional

cases also may have trabeculae of lamellar bone and calcified spherules. Myxoid

degeneration, aneurysmal bone cyst changes and multinucleated giant cells may be seen

in both variants.



Conservative resection is the treatment of choice with recurrence rates ranging from 30-

50% (hence the previous name “aggressive JOF”). Transformation into a sarcoma has

not been reported.



Fibrous Dysplasia

Fibrous dysplasia (FD) is a fibro-osseous lesion likely arising from altered

osteoprogenitor fibroblast-like cells. FD typically arises in the first or second decade as a

slowly enlarging painless expansion of the involved bone(s). There is no sex or rade

predilection. Lesions tend to stop growing at the onset of puberty or skeletal maturation.

FD can be classified into monostotic, polyostotic, craniofacial and syndromic types. The

syndromic types arise with polyostotic FD and include McCune-Albright syndrome (triad

of polyostotic FD, café au lait spots and multiple endocrinopathies) and Mazabraud

syndrome (polyostotic FD and intramuscular mxyomas). All types are associated with an

activating mutation of the GNAS1 gene (alpha subunit of a G stimulatory protein) on

chromosome 20q13 resulting in constitutive activation of adenylyl cyclase leading to a

persistent increase in cAMP which, in turn, activates one or more downstream genes.



Approximately 25% of patients with FD have involvement of the craniofacial bones.

Craniofacial FD is used to describe maxillary FD because there is often continuous

involvement of adjacent bones such as the zygoma, sphenoid, temporal, orbital, and

frontonasal bones. Because of this, they are not considered true polyostotic FD.

Although multiple contiguous bones are often involved, craniofacial FD is usually a

unilateral process. Mandibular FD can be monostotic or polyostotic.



The radiologic appearance is characteristic and varies with the stage and amount of

mineralized matrix. Early lesions are lytic but the classic finding in established lesions is

a ground glass appearance with indiscernible borders that blend into the adjacent

uninvolved bone. This contrasts with the imaging findings of OF and is very helpful in

the differential diagnosis of these two. Radiolucencies may develop and are typically

associated with secondary aneurismal bone cyst changes. At surgery, the abnormal bone

is difficult to separate from native bone and is often received as gritty fragments.



The histologic appearance of FD depends on the stage of the lesion and FD of the

craniofacial bones can show changes that are not seen in other bones. Early FD is

characterized by irregular (curvilinear; Chinese characters) trabeculae of woven bone

associated with a moderately cellular, haphazard proliferation of bland spindled-ovoid

fibroblasts. Lesional bone fuses with adjacent non-lesional bone. Unlike in the long

bones, FD of craniofacial bones can undergo maturation to lamellar bone with a decrease

in stromal fibroblast cellularity. Other changes that may be seen that are not seen in long

bone FD include prominent osteoblast riming and cementum-like calcifications. One

feature that is seen in FD but typically not in OF is histologic uniformity from field to

field within a given tumor. In contrast, OF demonstrate histologic variety with differing

amounts and quality of both the fibrous stromal and mineralized elements.



Osseous Dysplasia

While osseous dysplasias (OD) are the most common of the BFOLs, they are the least

likely to be seen as a surgical pathology specimen. They include both non-hereditary

(reactive) and hereditary forms. These lesions occur only tooth-bearing bones and are

likely related to the periodontal ligament structures. Because of the distinct predilection

for women, hormone-related abnormalities may play a role in OD pathogenesis. The OD

subtypes include periapical, focal, florid and familial gigantiform cementoma (FGC).

Each type has unique demographic, radiologic and clinical features which allow for their

distinction (Table). However, they all appear similar histologically consisting of

“dysplastic” or disordered production of bone/cementum and fibrous tissue. The

histologic features overlap with OF and FD and depend on the stage of the lesion. Early

lesions contain a more cellular spindle cell fibroblastic stroma with varying amounts of

cementum-like calcifications and trabeculae of woven bone. In older lesions, calcified

matrix elements predominate with less cellular and more collagenized stroma. Lamellar

bone with Paget-like reversal lines may be seen and stromal multinucleated giant cells

can be seen in any stage of the lesion. Compared to OF and FD, the stroma tends to be

more vascularized. OD usually do not require surgical intervention but do require

follow-up to monitor growth of the lesions. In the rare cases that do go to surgery, the

lesion tends to be removed as gritty fragments that are hard to separate from the native

bone, similar to FD, but unlike OF which shells out easily.



Age Race Symptoms Focality Location Expansion

Periapical 30-50 B>>>>W None Uni- or multifocal Ant mand No

Focal 30-50 B>>W None Unifocal Post mand No

Florid 30-50 B>>>>W Pain, none Multifocal; 2+ quadrant Post mand No; Minor

FGC Young B=W Pain, none Multifocal; 2+ quadrant All Yes



Differential Diagnosis of BFOLs

Several pathologic processes that involve the jawbones can have some histologic, clinical

and radiologic overlap with the BFOLs. These include neoplastic (benign and

malignant), inflammatory, metabolic and other giant cell rich lesions. Neoplastic lesions

include low-grade osteosarcoma, osteoid osteoma, osteoblastoma/cementoblastoma,

intra-osseous meningioma and odontogenic fibroma. Several forms of chronic

osteomyelitis can involve the jawbones inducing a fibro-osseous response. The presence

of inflammatory foci should raise this possibility, especially if a history of tooth abscess

or prior surgery is identified. Chronic renal failure or parathyroid disease should raise the

consideration of renal osteodystrophy and brown tumor, respectively. Paget disease of

bone can histologically resemble BFOL but has characteristic clinical and radiologic

findings. Finally, some giant cell lesions such as central giant cell lesion (formerly giant

cell reparative granuloma), cherubism and aneurysmal bone cyst can have a more fibrous

spindle cell stroma with reactive bone formation.



REFERENCES

1. Alawi F. Benign fibro-osseous diseases of the maxillofacial bones. A review and

differential diagnosis. Am J Clin Pathol. 2002. 118(suppl 1):S50-S70.

2. El-Mofty S. Psammomatoid and trabecular juvenile ossifying fibroma of the

craniofacial skeleton: Two distinct clinicopathologic entities. Oral Surg Oral Med Ora

Pathol Oral Radiol Endod. 2002;93:296-304.

3. Eversol R, Su L, El-Mofty S. Benign fibro-osseous lesions of the craniofacial complex.

A review. Head and Neck Pathol. 2008;2:177-202.

4. Speight PM, Carlos R. Maxillofacial fibro-osseous lesions. Curr Diag Pathol.

2006;12:1-10.



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