Workshop III: Phage Library Construction and Screening
Presentation Title:
Landscapes against Landscapes. Targeting Complex Biological Systems: Bacteria, Spores and
Tumor Cells, with Multivalent Phage Probes.
Speaker:
Valery A. Petrenko
Institution:
Department of Pathobiology, Auburn University, AL
Abstract:
Multivalent “landscape” display of random peptides (8-9-mers) on all 4,000 copies of the major
coat protein pVIII in phage fd-tet is achieved by splicing of degenerated DNA sequences into the
gene VIII of vectors f8-1, f8-5 and f8-6 in various registers. Hundreds of clones from the
landscape libraries were sequenced to characterize their diversity, evolution and censoring.
Biopanning of the landscape libraries against complex biological systems: bacterium Salmonella
typhimurium, Sterne spores of Bacillus anthracis, prostate and glioma cancer cells resulted in
selection of unique families of phage-binders specific for the target system. Using the landscape
phage for targeting the complex biological systems allows selecting variety of candidates that bind
different receptors—a rich source for subsequent screening of selective and strong binders.
Target-specific landscape phage were cross-inked and used as affinity absorbents for isolation and
identification of biomarkers.