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Thyroid and Parathyroid Function

PHTY 615

Introduction

• Hormones secreted from the thyroid gland involved in controlling

metabolism

– Also work synergistically with other hormones to promote normal growth and

development

• Parathyroid glands essential in regulating calcium homeostasis and are

important in maintaining proper bone mineralization

• Problems with thyroid and parathyroid glands often treated by

pharmacologic methods

Function of the Thyroid Gland

• Gland on either side of the trachea in the anterior neck

– Bilateral lobes connected by a central isthmus

– Rich vascular supply and extensive nerve supply from the sympathetic

NS

• Thyroid gland synthesizes thyroxine and triiodothyroxine

Normal Thyroid Gland

Synthesis of Thyroid Hormones

• First step is adding iodine to the residues of AA tyrosine

• 2 iodinated tyrosines combine to create thyroid hormones

– Monoiodotyronsine + Diiodotyrsine = Triiodotyronsine (T3)

– Diiodotyronsine + Diiodotyrsine = Thyroxine (T4)

• Plasma levels of T4 much higher than T3 but:

– T3 may exert most of physiologic effects

– T4 may be precursor to T3

• T3 converted to T4 in peripheral tissue

– Both T4 and T3 required for normal physiologic function

Iodine uptake

• + -

Na /I symport protein controls serum I- uptake

• Based on Na+/K+ antiport potential

• Stimulated by TSH

• Inhibited by Perchlorate



Synthesis of Thyroid Hormones

• Thyroid follicle cells take up and concentrate iodide in the bloodstream

– Must be a sufficient amount of iodine in the diet to provide iodide needed for

thyroid hormone function

• Thyroid cells also make protein called thyroglobulin (TGB)

– 2 iodinated tyrosine residues combine with TGB to make T4 and smaller amounts

of T3

– TGB lysed from the iodinated tyrosines before released into the bloodstream







Regulation of Thyroid Hormone Release

• Controlled by hypothalamic-pituitary system

• Thyrotropin-releasing hormone (TRS) from hypothalamus

stimulates release of thyroid-stimulating hormone (TSH) from

the anterior pituitary

• TSH stimulates release of T3 and T4

• Negative feedback system controls TSH release from the

anterior pituitary gland

Thyroid Hormone Control

Control of T3 and T4 Release

Under normal conditions, decreases in T3/T4 cause TRH release from the hypothalamus (HPT) which

then causes TSH release from the pituitary (PIT).

TSH then stimulates:

1) T3/T4 synthesis and secretion

2) thyroid gland growth.

When T3/T4 levels increase, negative feedback shuts off TRH and TSH secretion.



Physiologic Effects of Thyroid Hormone

• Affect wide variety of peripheral tissue

• T4 and T3 may act directly on cell or facilitate function of other hormones

• Thermogenesis

– T4 and T3 increase basal metabolic rate and subsequent heat production

– Important in maintaining adequate body temperature during exposure to the cold

– Increase in thermogenesis achieved by thyroid hormone stimulating various

tissues

• Skeletal mm, cardiac mm, liver, kidneys



Physiologic Effects of Thyroid Hormone

• Growth and development

– Thyroid hormones facilitate normal growth and development

• Stimulate release of growth hormone and enhances its effect on peripheral tissue

– Thyroid hormones directly enhance development of many physiologic systems

• Especially skeletal mm and CNS

– When thyroid hormones not present:

• Severe growth restriction and mental retardation ensues

Physiologic Effects of Thyroid Hormone

• Cardiovascular effects

– Thyroid hormones increase heart rate and myocardial contractility

• Increase cardiac output

– Unclear how thyroid hormones increase myocardial sensitivity to other hormones

• Norepinephrine and Epinephrine

• Metabolic effects

– Increase intestinal glucose absorption

– Increase activity of several enzymes involved in carbohydrate metabolism

– Enhances lypolysis by increasing response of fat cells to other lypolytic hormones

Mechanism of Action of Thyroid Hormones

• Thyroid hormones enter the cell and bind to specific receptors in the cell

nucleus

– Thyroid hormone receptors act as DNA transcription factors that bind to specific

DNA sequences that regulate gene expression

– Alter protein synthesis in the cell

• Most if not all of thyroid hormone effects are secondary to altered protein

production

– Proteins may increase transport of specific substances across cell membrane or

new protein may be directly involved in metabolic pathway

Treatment of Thyroid Disorders

• Two primary categories

– Hyper and Hypothyroidism

• Several subtypes

– Hyperthyroidism

• Primary: Graves disease, thyroid adenoma

• Secondary: Induced by excessive hypothalamic or pituitary stimulation

– Hypothyroidism

• Primary: Genetic deficiency of enzymes that synthesize thyroid hormones

• Secondary: Hypothalamic or pituitary deficiencies, Cretinism (childhood hypothyroidism),

Myxedema (Adult hypothyroidism)



Hypothyroidism and its Treatment:

Hypothyroidism and its Treatment:

When the pituitary can't make TSH there is no signal to the thyroid gland to make T3/T4. Thus secondary

hypothyroidism is (i.e. pituitary- mediated) associated with decreased T3/T3 AND TSH and thyroid

atrophy.



Clinical Manifestations

• Hyperthyroidism

– Nervousness

– Weight loss

– Diarrhea

– Tachycardia

– Insomnia

– Increased appetite

– Heat intolerance

– Wasting

– Goiter

– Anorexia





• Hypothyroidism

– Lethargy/slow cerebration

– Weight gain

– Constipation

– Bradycardia

– Sleepiness

– Cold Intolerance

– Weakness

– Dry, course skin

– Facial edema



Hyper- thyroidism Features:

Hypo- thyroidism



Myxedema



Features:

Hyperthyroidism

• Thyrotoxicosis:

– Increased secretion of thyroid hormones

– Usually enlarged thyroid gland

• Toxic goiter = Graves disease

• Thought to be immune system problem

• Treatment

– Ablation of the thyroid gland

• Surgical removal or administration of radioactive iodine

Antithyroid Agents

• Directly inhibit thyroid hormone synthesis

• Propylthiouracil (Propyl-Thyracil), Methimazole (Tapazole)

• Drugs inhibit thyroid peroxidase enzyme needed to prepare iodide for

addition to tyrosine

• Also prevent coupling of tyrosine residues to thyroglobulin molecule

– Propylthiouracel also blocks conversion of T4 to T3 in peripheral tissue

• Adverse effects

– Skin rash and itching (mild)

– High doses may cause symptoms associated with hypothyroidism

Iodide

• Large doses of iodide (>6mg/day) causes a rapid decrease in thyroid

function

• In sufficient amounts, iodide inhibits virtually all steps involved in thyroid

hormone biosynthesis

– High iodide levels limit uptake of iodide into thyroid follicle cells, inhibits formation

of T4 and T3, and decreases secretion of completed hormones

• Effect of Iodide lasts ~2 weeks

– Iodide used temporarily for individuals awaiting thyroidectomy

Radioactive Iodine

• Selectively destroys thyroid tissue in Graves disease

• Radioactive iodine administered orally and rapidly sequestered

in thyroid gland

• Begins to emit beta radiation destroying thyroid follicle cells

• Patients must subsequently take thyroid hormone supplements

Beta-Adrenergic Blockers

• Adjunctive treatment to thyrotoxicosis

• BB do not lower thyroid hormone levels

• BB help suppress symptoms such as tachycardia, palpitations, fever,

restlessness

• BB may be helpful in severe, acute, exacerbations of thyrotoxicosis

• BB also administered preoperatively to control symptoms

• Acebutolol, atenolol, metoprolol, nadolol, propanolol, timolol

Hypothyroidism

• Many causes

– Idiopathic, autonomic, lymphocytic destruction, congenital impairment,

low dietary iodine intake, genetic

– Goiter may also occur but for different reasons

• Decrease dietary iodine

– TSH stimulates production of thyroglobulin

– Thyroid hormone incomplete

– No negative feedback and thyroglobulin production continues

– Primary treatment

• Thyroid hormone replacement therapy

Thyroid Hormones

• Natural or synthetic analogs necessary in most forms of hypothyroidism

• Administration of thyroid hormones especially important in infants and

children

– Adequate amounts needed for normal physical and mental development

• Thyroid hormone replacement is likewise necessary following

thyroidectomy or pharmacological ablation

• Thyroid hormone maintenance may be beneficial for patients in

preliminary or subclinical phase of hypothyroidism

– May prevent full blown hypothyroidism

• Main side effect is symptoms of hyperthyroidism

Hypothyroidism: Treatments

-Sodium levothyroxine (Synthroid®-T4)



-Sodium liothyronine (Cytomel®-T3)

-4-times as potent as T4, more rapid onset of action,

same efficacy



Parathyroid Gland Anatomy

• Four Parathyroid glands are usually found posterior to the thyroid gland

• Total weight of parathyroid tissue is about 150mg

• Parathyroid hormone (PTH) is made by these glands

Function of the Parathyroid Glands

• Parathyroid hormone (PTH)

– Polypeptide synthesized within cells of parathyroid glands

• Calcium concentration in bloodstream controls PTH release

• Decrease in CA2+ concentration increases PTH release and increased

CA2+ concentration decreases PTH release

• PTH increases blood CA2+ level by altering CA2+ metabolism in bone,

kidneys, and GI tract

Function of the Parathyroid Glands

• PTH increases bone resorption, liberating CA2+

– Enhances development and action of osteoclasts

• PTH increases renal resorption of CA2+

• PTH lastly increases absorption of CA2+ in the GI tract

– Caused by interaction between PTH and Vitamin D metabolism

– PTH increases conversion of Vitamin D to calcitriol

– Calcitriol stimulates CA2+ absorption from the GI tract

• PTH crucial in maintaining adequate levels of calcium in the body

• PTH works with Calcitonin and Vitamin D in regulating CA2+

homeostasis



Regulation of Bone Mineral Homeostasis

• Bone

– Provides rigid framework for body and readily available and easily interchangeable

CA2+ pool

– Balance between bone formation and resorption important

– CA2+ and phosphate needed for bone to maintain rigidity

– Excessive resorption of these minerals result in bone demineralization

• Bone at increased risk for failure (fracture)

– Balance between bone resorption and formation controlled by complex interaction

between local and systemic factors

– Several hormones regulate bone formation and help maintain adequate calcium

levels

Parathyroid Hormone

• Discussed previously

– Increase in PTH = Increase in blood CA2+ levels

– High PTH levels accelerates bone breakdown

– Low-Normal PTH levels may enhance bone formation

• May have implications for treatment of osteoporosis





Vitamin D

• Steroid like hormone from dietary sources or synthesized in skin from cholesterol

derivatives in the presence of ultraviolet light

• Vitamin D produces several metabolites important in bone mineral homeostasis

• Vitamin D derivatives such as 1,25 Dihydroxyvitamin D3 increases serum CA2+ and

phosphate levels by increasing CA2+ and phosphate absorption in GI tract and

decreases renal CA2+ and phosphate excretion

• Overall effect of Vitamin D is to enhance bone formation by increasing levels of

primary minerals (CA2+ and phosphate)

• Also suppresses synthesis and release of PTH





Synthesis, Release & Activity of Active Vitamin

D

• Vitamin D3 is may be obtained from the diet or made in the skin

• It is converted to the active form (1,25-OH-D3 by sequential enzymatic reactions in

the liver and kidney (stimulated by PTH)

• Vitamin D3 stimulates intestinal calcium uptake, increased bone calcium resorption &

increased kidney phosphate uptake

Calcitonin

• Secreted by cells located in the thyroid gland (C cells)

– Calcitonin is physiologic antagonist of PTH

• Calcitonin lowers blood calcium and stimulates bone formation

– Increases incorporation of CA2+ into skeletal storage

• Renal excretion of CA2+ also increased by Calcitonin effect on the

kidney

• Effect of Calcitonin on bone mineral metabolism relatively minor

• Calcitonin does have important therapeutic function

– Pharmacological doses may be helpful in preventing bone less

Calcium Metabolism:

Hormonal Regulation of Calcium and Phosphate Balance

• Decreased Plasma Calcium Causes:

• Increased PTH

• Resulting in mobilization of bone Ca & phosphate, increased renal phosphate excretion & Ca retention

and increased Vitamin D3 synthesis

• The outcome is a rise in plasma Ca levels & maintenance of normal phosphate levels





Summary

• PTH & calcitonin release are regulated by plasma Ca levels

• Bone Ca & phosphate serve as a ready reserve for

maintenance of plasma levels

• Bone, kidney & intestine participate in the regulation of plasma

calcium

• PTH, Vitamin D, & calcitonin balance plasma [Ca++] for bone

synthesis, muscle contraction, & cell signaling

• Endocrine diseases result from pathway or glandular hypo or

hyper secretion



Pharmacological Control of Bone Mineral

Homeostasis

• Blood CA2+ levels must be maintained within fairly limited range to

ensure adequate supply of calcium for various physiologic functions

– Normal range = 8.6-10.6 mg/100 ml

• Plasma levels 12 mg/100 ml depresses nervous system function

– Sluggish, lethargy, possible coma

Pharmacological Control of Bone Mineral

Homeostasis

• Chronic disturbances in CA2+ homeostasis can also produce problems

with bone calcification

• Various metabolic diseases can alter blood CA2+ levels leading to hypo

or hypercalcemia

• Pharmacological methods used to help control bone mineral levels in the

bloodstream and maintain adequate bone mineralization



Calcium Supplements

• Administered to ensure adequate calcium levels in the bloodstream

• Calcium supplements used to help prevent bone loss in conditions such as osteoporosis

– Can’t prevent osteoporosis alone but helpful when combined with other treatments such as

estrogen replacement

• Dose of calcium supplement should make up the difference between dietary calcium intake and

established guidelines

– Amount depends on amount to dietary calcium, age, gender, hormonal and reproductive status

• Excessive doses must be avoided to prevent hypercalcemia

– Constipation, drowsiness, fatigue, headache

– More pronounced: Confusion, irritability, cardiac arrhythmias, hypertension, nausea, vomiting,

muscle and bone pain



Vitamin D

• Precursor for other compounds that increase intestinal absorption and

decrease renal excretion of CA2+ and phosphate

• Used to increase blood CA2+ and phosphate levels and enhance bone

mineralization

• Vitamin D analogs such as calcitriol combined with CA2+ supplement to

help treat:

– Postmenopausal osteoporosis

– Bone loss with antiinflammatory steroids

Vitamin D

• Vitamin D is fat soluble

– Excessive doses can accumulate in body and lead to toxicity

• Early signs = headache, increased thirst, decreased appetite, metallic taste,

fatigue, GI disturbance

• Increased toxicity = Hypercalcemia, HTN, cardiac arrhythmias, renal failure,

mood changes, seizures





Bisphosphonates

• Inorganic compounds that appear to adsorb directly to CA2+ crystals in

the bone and decrease bone resorption

– Inhibits osteoclast activity

• Bisphosphonates often used in Pagets disease

– Help prevent excessive bone turnover and promote adequate mineralization

• Used to inhibit abnormal bone formation

– Heterotopic ossification

Bisphosphonates

• Treatment of choice for prevention and treatment of bone loss

during prolonged administration of antiinflammatory steroids

• Used in postmenopausal women

• Adverse effects

– Tenderness and pain over site of bony lesions in Pagets disease

– Fracture risk with excessive doses

– GI disturbances







Calcitonin

• Derived from synthetic sources to mimic effects of endogenous hormone

• Promotes bone mineralization

• Used to treat hypercalcemia and decrease bone resorption in Pagets

disease

• Used in postmenopausal and glucocorticoid-induced osteoporosis

• Aerosolized versions of Calcitonin now available

– Oral delivery difficult because absorbed poorly from the GI tract

Estrogen Therapy

• Critical in maintaining adequate bone mineralization in women

• Used with other adjunct treatments

– Calcium supplement, Calcitonin, Calcitriol, Bisphosphonates

• May increase risk of breast and uterine cancer

• Selective Estrogen Receptor Modulators (SERMS)

– Activates receptors in certain tissues and blocks effect in others

– Primary SERM used to prevent osteoporosis is Reloxifene (Evista)

• Binds to and activates estrogen receptors in bone

– Prevents bone loss and demineralization

• Reloxifene blocks estrogen receptors on breast and uterine tissue



Special Concerns for Rehabilitation Patients

• PTs need to be concerned with side effects of different drugs

• Thyroid disorders

– Excessive doses can cause opposite disorder

• Bone disorders

– Hypercalcemia can be detected by ECG

• Can prevent life threatening disorder

• Exercise

– Helps to stimulate bone formation



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