Figure S1. Comparative neutralization of SIVmac239 and four SIV variants
by SIV Env-specific monoclonal antibodies. (A) rhesus monoclonal antibody
(rhMab) 3.10A. (B) rhMab 3.5F. (C) rhMab 3.11E. (D) rhMab 3.11H. (E) rhMab
1.9C. (F) rhMab 1.10A. (G) rhMab C26. (H) mouse monoclonal antibody KK41.
HEK293T produced virus for each cloned SIV variant and, separately, the
parental SIVmac239 was incubated for 1 h with each rhesus monoclonal anti-
gp120 antibody or separately with a mouse monoclonal anti-SIV gp41 antibody.
Then, C8166-SEAP cells that contain a stably integrated, Tat-inducible secreted
alkaline phosphatase (SEAP) reporter gene were added. SEAP activity was
measured 72 hours later with a Tropix Phospha-LightTM kit. A lower percentage
of SEAP activity is indicative of neutralization while 100 percent SEAP activity
indicates the lack of neutralization of virus infectivity. SIVmac239 = black square;
SIVmac316=gray square; SIVN2 = aqua triangle; SIVN5 = crimson square;
SIVN7 = yellow circle; SIVN8 = bluegray diamond.