Curriculum Vitae

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					                             NAYUN KIM, PH.D.

324 Aaron Circle                                    nk43@notes.duke.edu
Durham, NC 27713                                    (919) 684 - 0042



PROFILE OVERVIEW
Doctoral research molecular biologist with practical, laboratory and
management experience in the areas of molecular genetics, molecular
immunology, and microbiology with research focus on the DNA repair pathways
and genomic instability.


EDUCATION

August 2000 Ph.D. Biochemistry and Molecular Biology
            Thesis title “Transcription and DNA repair in immunoglobulin gene
            somatic hypermutations”
            Department of Biochemistry and Molecular Biology, University of
            Chicago, Chicago IL

June 1994    B.S. Chemistry
             University of Chicago, Chicago IL

WORK EXPERIENCE

Sept 2006 to present     Research Associate Senior
                   Duke University Medical Center
                   Department of Molecular Genetics and Microbiology

                   My advisor Dr. Sue Jinks-Robertson has relocated to Duke
                   University Medical Center in Sept 2006. After helping with
                   the move and set-up of the new lab at the new location, I am
                   continuing with my research in genomic instability in
                   Saccharomyces cerevisiae.

Sept 2003~Sept 2006      Postdoctoral Fellow
                  Emory University
                  Department of Biology. Atlanta, GA
                  I am taking a molecular genetic approach to study
                  transcription-associated DNA mutagenesis and
                  recombination, using the model system Saccharomyces
                  cerevisiae. I am also studying the molecular basis of “At-
                  Risk” sequence motifs implicated in tumorigenesis using a
                  yeast model system.


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                  Advisor: Professor Sue Jinks-Robertson, Ph.D.
Sept 2000 – Aug 2003     Postdoctoral Fellow
                  University of Pittsburgh
                  Department of Pharmacology Pittsburgh, PA
                  I created recombinant expression and purification protocols
                  for a novel human DNA polymerase using human tumor cell
                  line HELA cells, Sf9 insect cell system, and E. coli bacterial
                  cell system.
                  Advisor: Professor Richard D. Wood, Ph.D.,F.R.S.

1994 – 2000                Ph.D. Candidate
                    University of Chicago
                    Dept. of Biochem. and Mol. Bio. Chicago, IL
                    My graduate research focused on the molecular process
                    involved in the affinity maturation and repertoire
                    diversification of activated B lymphocytes.
                    Advisor: Professor Ursula Storb, M.D

MOLECULAR, BIOCHEMICAL, AND CELL BIOLOGICAL TECHNIQUES

              RNA, DNA manipulation, PCR, RT-PCR, Real-time Quantitative
              RT-PCR, electrophoresis, Southern, Northern and Western
              analysis, Fluorescent Activated Cell Sorting analysis, DNA
              polymerase activity assay using radio-labeled nucleic acid, protein
              Affinity purification, Fluorescent microscopy, Ion exchange Fast
              Protein Liquid Chromatography (FPLC), human and murine primary
              cell culturing, transgenic mice, di-deoxy terminating Sequencing,
              and SSCP (single strand conformation polymorphism) analysis
              using radio-labeled nucleic acid.

TEACHING EXPERIENCE

Fall 2004 & 2005    Emory University Atlanta, GA
                    Teaching Assistant/ Biology 141
                    Instructor for the laboratory exercises in introductory cell and
                    molecular genetics course.

Fall 1999           University of Chicago Chicago, IL
                    Teaching Assistant/ BioSci 181
                    Assistant for the introductory cell and molecular biology
                    course for advanced undergraduate science major students.

Fall 1995           University of Chicago Chicago, IL
                    Teaching Assistant/ BMB 311
                    Protein Structure and Function – Graduate course in the
                    introduction to the biochemical study of protein.


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PUBLICATIONS

Kim, N. and Jinks-Robertson, S. 2009 “dUTP incorporation into genomic DNA is
linked to transcription in yeast.” Nature 459:1150 – 1153.

Kim, N., Abdulovic, A., Gealy, R., Lippert, M., and Jinks-Robertson, S. 2007
“Transcription-associated mutagenesis in yeast is directly proportional to the
level of gene expression and influenced by the direction of DNA replication” DNA
repair 6:1285 – 1296.

Abdulovic, A., Kim, N., and Jinks-Robertson, S. 2006 “ Mutagenesis and the
three R’s in yeast” DNA repair 5:409 – 21.

Marini, F., Kim, N., Schuffert, A., and Wood, R. 2003 “POLN, a nuclear POLA
family DNA polymerase homologous to the DNA cross-link sensitivity protein
Mus308” The journal of biological chemistry 278:32014 – 32019.

Michael, N., Shen, H. M., Longerich, S., Longacre, A., Kim, N., and Storb, U.
2003 “’The E-box motif, CAGGTG, is an enhancer of somatic hypermutation
without enhancing transcription.” Immunity 19:235 – 242.

Kim, N., Martin, T. E., Simon, M. C., and Storb, U. 2003 "The transcription
factor Spi-B is not required for somatic hypermutation" Molecular Immunology
39:577 – 583.

Michael, N., Martin, T. E., Nicolae, D., Kim, N., Padjen, K., Zhan, P., Nguyen, H.,
Pinkert, C., and Storb, U. 2002 “Effects of sequence and structure on the
hypermutability of immunoglobulin genes.” Immunity 16:123 – 134.

Shen, H. M., Michael, N.,Kim, N., and Storb, U. 2000 “The TATA binding
protein, c-MYC and survivin genes are not somatically hypermutated, while Ig
and BCL6 genes are hypermutated in human memory B cells.” International
Immunology 12:1085 – 1093.

Storb, U., Shen, H. M., Michael, N., and Kim, N. 2000 "Somatic hypermutation
of immunoglobulin and non-immunoglobulin genes" Philosophical Transactions
of the Royal Society 356:13 – 20.

Storb, U., Peters, A., Kim, N., Shen, H.M., Bozek, G., Michael, N., Hackett, Jr,
J., Klotz, E., Reynolds, J.D., Loeb, L.A., and Martin, T.E. 1999 “Molecular
aspects of somatic hypermutation of immunoglobulin genes.” Cold Spring
Harbor Symposia on Quantitative Biology 64:227 – 34.

Kim, N., Bozek, G., Lo, J. C., Storb, U. 1999 “Different mismatch repair
deficiencies all have the same effects on somatic hypermutation: intact primary

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mechanism accompanied by secondary modifications.” Journal of Experimental
Medicine 190:21-30.

Kim, N., and Storb, U. 1998 “The role of DNA repair in somatic hypermutation of
immunoglobulin genes.” Journal of Experimental Medicine 187:1729-1733.

Storb, U., Peters, A., Klotz, E., Kim, N., Shen, H. M., Kage, K., Rogerson, B.,
Martin, T. E. 1998 “Somatic hypermutation of immunoglobulin genes is linked to
transcription.” Current Topics in Microbiology and Immunology 229:11-18

Storb, U., Peters, A., Klotz, E., Kim, N., Shen, H. M., Rogerson, B., and Martin,
T. E. 1998 “Cis-acting sequences that affect somatic hypermutation of Ig
genes.” Immunological Reviews 162:153-160.

Kim, N., Kage, K., Matsuda, F., Lefranc, M.-P., and Storb, U. 1997 “B
lymphocytes of xeroderma pigmentosum or Cockayne syndrome patients with
inherited defects in nucleotide excision repair are fully capable of somatic
hypermutation of immunoglobulin genes.” Journal of Experimental Medicine
186:413-419.

Zent, C., Kim, N., Hiebert, s., Zhang, D. E., Tenen, D. G., Rowley, J. D., and
Nucifora, G. 1996 “Rearrangement of the AML1/CBFA2 gene in Myeloid
leukemia with the 3;21 translocation : Expression of co-existing multiple chimeric
genes with similar functions as transcriptional repressors, but with opposite
tumorigenic properties.” Current Topics in Microbiology and
Immunology 211:243-252.

ABSTRACTS and PRESENTATIONS

“The role of replication orientation and endogenous DNA damage in
Transcription-associated mutagenesis in Saccharomyces cerevisiae” Gordon
Research Conference on Mutagenesis 2006

“Probing Transcription stimulated mutagenesis (TAM) in Saccharomyces
cerevisiae using Tetracycline-regulative Dual Expression System” Gordon
Research Conference on Mammalian DNA Repair 2005

“Somatic Hypermutation of Ig genes and DNA mismatch repair” Keystone
Symposium on B cell immunobiology and disease 1999

“Somatic Hypermutation of Ig genes and transcription” Keystone Symposium on
Transcription regulation 1997

HONORS, AWARDS

1998 and 1999       Committee on Cancer Biology Travel Grant

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1998              Howard Tager Memorial Travel Grant
June 1994         General Honors at Graduation (B.S.)
June 1994         Induction to Phi Beta Kappa
REFERENCES

  1. Professor Sue Jinks-Robertson, Ph.D.
     Duke University Medical Center
     Department of Molecular Genetics and Microbiology
     228 Jones Building, Research Drive Box3020
     Durham, NC 27710
     Phone: (919) 681 – 7273, Fax: (919) 684 – 2790
     sue.robertson@duke.edu

  2. Professor Richard D. Wood, Ph.D.
     University of Pittsburgh
     Pittsburgh Cancer Institute
     5117 Centre Avenue, Research Pavilion, Suite 2.6
     Pittsburgh, PA 15213
     Phone: (412) 623 – 7762, Fax: (412) 623 – 7761
     rdwood@pitt.edu

  3. Professor Ursula Storb, M.D.
     University of Chicago
     Department of Molecular Genetics and Cell Biology
     920 E. 58th Street CLSC1061
     Chicago, IL 60637
     Phone: (773) 702 – 4440
     stor@midway.uchicago.edu

  4. Professor Gray Crouse, Ph.D.
     Emory University Department of Biology
     1009 Rollins Research Center
     1510 Clifton Road
     Atlanta, GA 30322
     Phone: (404) 727 – 4236, Fax: (404) 727 – 2880
     gcrouse@biology.emory.edu




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