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					Supplemental Table 2: Alleles in microsatellite loci in single cells of 4 HMs with two cell populations

HM142                    D6S105          D6S2443         Interpretation
Paternal                 117/119         175
Maternal                 121/123         193/197
HM, tissue               117/123 +       175/197 +       P1M + P1P1
                         117             175
HM, single cells (9)a    117/123         175/197         P1M
                 (1)     123             165/197         Contamination with alien DNA
HM173
Paternal                 117/123         165/175
Maternal                 117/121         165/167
HM, tissue               121/123 +       165/175 +       P1M + P1P2
                         117             165
HM, single cells (1)     123             165/175         Possible P1M, drop-out of one allele
                 (1)     121             165             Possible P1M, drop-out of two alleles
                 (1)      -b             165             Possible P1P2, no PRC product in D6S105
                 (5)     117/121/123     165/175         Possible P1M or P1P2, contamination with DNA
                                                         from HM tissue
                  (2)    117/121/123     165             Possible P1M or P1P2, contamination with DNA
                                                         from HM tissue, and drop-out of one allele
                  (1)    117/121         165/175         Possible P1M or P1P2, contamination with DNA
                                                         from HM tissue, and drop-out of one allele
                  (3)    117/123         165/175         Possible P1M or P1P2, contamination with DNA
                                                         from HM tissue, and drop-out of two alleles
                  (1)    117             165/167/175     Contamination with alien DNA
                  (3)    117             165/167         Contamination with alien DNA
                  (1)    113/115/117     165/167/175     Contamination with alien DNA
                  (1)    115/117         165/167         Contamination with alien DNA
                  (1)    115/117         165             Contamination with alien DNA
                  (1)    117/121/123     165/167/175     Contamination with alien DNA
                  (3)    -               -               No PRC product
HM539
Paternal                 121             165/175
Maternal                 117             169/193
HM, tissue               117/121 +       165/169 +       P1M + P1P2
                         121             165
HM, single cells (9)     117/121         165/169         P1M
                 (1)     121             165             P1P2
                 (2)     121             165/169         Possible P1M, drop-out of one allele
                 (3)     -               165/169         Possible P1M, no PRC product in D6S105
HM652
Paternal                117/123        167/195
Maternal                117            197
HM, tissue              117            167/197+167 P1M + P1P2
HM, single cells 13)    117            167/197        P1M
                 (1)    117            167            P1P2
                 (1)    -              167            Possible P1P2, no PRC product in D6S105
                 (1)    117            167/189/197 Contamination with alien DNA
                 (1)    117/123        167/197        Contamination with alien DNA
                 (1)    -              -              No PRC product
a) The numbers in the parentheses refer to the number of cells counted with the respective allele
combination
b) -: No signal

				
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posted:11/30/2011
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