SIXTH FRAMEWORK PROGRAMME

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					SIXTH FRAMEWORK PROGRAMME


[PRIORITY: Specific measures in support International Co-operation – Western
Balkan countries]


Proposal No. PL 509160


Area: FP6-2002-INCO-WBC-1


CONTRACT No INCO-CT-2004-509160


Specific Targeted Project: Sava River Basin: Sustainable Use, Management and
Protection of Resources


Acronym: SARIB


Duration of project: 1.8.2004-31.7.2007


Project Co-ordinator: Dr. Radmila Milačič




                    THIRD YEAR REPORT (1.8.2006 – 31.7.2007)




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Table of Contents                                                                   Page
Summary and Introduction                                                            3
Project objectives                                                                  3
List of partners                                                                    4
Workpackage list                                                                    5
List od deliverables                                                                5
Report on work done – general remarks                                               7
Workpackage 1: Coordination                                                         9
Workpackage 2: Data Base and Tools                                                  11
Workpackage 3: Development and Validation of Specific Tools                         16
WP3.A Pollution of sediments and water cycling processes
Workpackage 3: Development and Validation of Specific Tools                         27
WP3.B Availability and impact of pollutants and biota
Workpackage 4: Integrated system for the management of the Sava River quality       30
Workpackage 5: Social, economic and governance benefits                             36
Workpackage 6: Dissemination                                                        40




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SUMMARY AND INTRODUCTION:

The Sava River (945 km) is the biggest tributary to the Danube River and has 95551 km2
large catchment. It extends over four countries, Slovenia, Croatia, Bosnia and Herzegovina
and Serbia. In the development of the river basin management plan all countries are already
collaborating under the International Commission for the Protection of the Danube River
(ICPDR) guidance. Until 1991, the methodological bases for data collection have been
reasonably unified over the catchment, but there is still luck of important aspects such as
ecological character of the river and its tributaries, inventory of pollution sources, dangerous
substances, socio-economic parameters and cost and benefit implications. For the later period
a lot of data are missing due to insufficient monitoring (financing, recent warfare) and weak
institutional and legal control over use of water and land resources of the Sava River
catchment. Many aspects of the river quality need scientific investigations. Furthermore, there
is a need to link the knowledge of river quality state and environmental and health risk with
pressures and their driving forces to propose efficient and beneficiary actions and measures
for protection and sustainable use of the river resources. In the project specific tools based on
combination of chemical analysis and biological effect methods were planned to be developed
and validated for the pollution of sediments and impact on water biota. Geographical
distribution of pollution was planned to be identified. An expert data and geographical
information system (GIS) was planned to be developed. Integrated prediction model about the
behaviour of hazardous chemical substances was planned to be integrated with the socio-
economic prediction model to serve as a base for the elaboration of scenario, remediation
measures and best practice techniques.


PROJECT OBJECTIVES:

Specific objectives of the SARIB project that should be achieved by the proposed work were:

 To collect and assess information on sources fluxes and concentration levels of studied
compounds in the Sava River Basin. For this purpose freshwater ecosystem, transboundary
and “hot spot” input of priority pollutants, validation of “passive samplers” for temporal
accumulation of pollutants, advanced analytical tools applied in trace analysis of pollutants,
biomarkers of subtoxic pollutant effects, biosensors for surface active substances, health
status of fish, abundant fish species in the Sava River, sediments as depository compartment
for persistent pollutants, bioaccumulation of metals in fish tissue, bioaccumulation of PAH‟s
and PC‟s in fish tissue were planned to be investigated. The information obtained from the
research activities was planned to be combined with conceptual model approaches to form a
base for developing assessment tools within the proposed project. Information on socio-
economic aspects was also planned to be collected.
 To develop methods and tools which give the possibility to distribute and/or remediate
critical loads (especially at “hot spots”) within the basin according to the ecological
vulnerability potential and optimal economic use based on cost-benefit analysis.
 To facilitate interdisciplinary collaboration between technical, natural and social
scientists and to ensure policy-relevant findings by the active incorporation of representatives
of users including industry, agriculture, environmental organizations and citizens groups at
the Sava River Basin level.

The project consortium consists of 11 partners that are involved in the work of 6 work-
packages. Lists of partners, workpackage list and list of deliverables are enclosed below.


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List of Partners:
Partner     Partner   Participant name        Participant   Country     Date enter   Date exit
Role*       No.                               short name                project**    project**
CO          1         Joţef Stefan            JSI           Slovenia    1            36
                      Institute, Ljubljana
CR         2          University of           UL FGG        Slovenia    1            36
                      Ljubljana, Faculty of
                      Civil and Geodetic
                      Engineering,
                      Ljubljana
CR         3          Institute Rudjer        RBI           Croatia     1            36
                      Bošković, Zagreb
CR         4          University of           PBF           Croatia     1            36
                      Zagreb, Faculty of
                      Food Technology
                      and Biotechnology,
                      Zagreb
CR         5          Hydro-Engineering       HEIS          Bosnia&     1            36
                      Institute, Sarajevo                   Herzegovi
                                                            na
CR         6          Mihajlo Pupin           Pupin         Serbia&     1            36
                      Institute, Belgrade                   Monteneg
                                                            ro
CR         7          University of Banja     FABL          Bosnia&     1            36
                      Luka, Faculty of                      Herzegovi
                      Agriculture, Banja                    na
                      Luka
CR         8          International Centre    ICS-UNIDO     Italy       1            36
                      for Science and High
                      Technology, Trieste
CR         9          University of Natural   BOKU Wien     Austria     1            36
                      Resources and
                      Applied Life
                      Sciences Vienna,
                      Institute for
                      Agrobiotechnology,
                      Tulln
CR         10         Norwegian Institute     NIVA          Norway      1            36
                      for Water Research,
                      Oslo
CR         11         Imos Geateh,            IMOS          Slovenia    1            36
                      Planners and            GEATEH
                      engineers, Ljubljana

*CO = Coordinator
 CR = Contractor



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    Work package list:
      Work-    Workpackage title                                                   Lead
     package                                                                   contractor No
       No.
        1      Coordination                                                           1
        2      Data base and tools                                                    2
        3      Development and validation of specific tools                           3
               3A: Pollution of sediments and water cycling processes
               3B: Availability and impact of pollutants on biota
        4      Integrated system for the management of the Sava                       8
               River quality
        5      Social, economic and governance benefits                               5
        6      Dissemination                                                          4




List of deliverables:
WP 1: Coordination
D 1.1: Yearly finance statements
D 1.2: Yearly financing plan
D 1.3: Inception report
D 1.4: Progress reports
D 1.5: Final report
WP 2: Data base and tools
D2.1: Report on collected data and information in all countries
D2.2 Structure of the GIS definition, data harmonisation
D2.3 GIS platforms development - on web and on CD
D2.4: Report on the evaluation and selection of pollution distribution modelling software
D2.5: Report on DPSIR indicators for the Sava river catchment

WP 3: Development and Validation of Specific Tools

WP 3A: Pollution of sediments and water cycling processes
D.3.1A: Database on the spatial and temporal extent of specific pollutants in sediments of the
Sava River Basin: nitrates, phosphates, Cd, Pb, Zn, Hg, Cu, As, Cr, Ni, sixteen parent PAHs,
five methyl-PAHs, seven the most toxic PCB congeners, atrazine and desethylatrazine, butyl
and phenyl derivatives of tin.
D.3.2A: Estimation of the extent of pollution and identification of the anthropogenic inputs
to the Sava River and its main tributaries.
D.3.3A: Identification of sources of dissolved inorganic carbon (DIC).
D.3.4A: Identification of "hot spots" of pollutant load.
D.3.5A: Characterisation of the toxic potential of sediment organic extracts and pore water
from Sava River by use of micro scale bioassays
D.3.6A: Establishment of integrated classification model for sediment contamination and
toxicity characterisation
D.3.7A: Final Report on sediments.
D.3.8A: Research papers in peer-reviewed journals on sediments; Guidelines on sediment
contamination and toxicity characterisation.


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WP 3B: Availability and impact of pollutants on biota
D.3.9B: Database on the incidence of diseased feral fish in Sava River study area
D.3.10B: Database on the level of specific priority pollutants (metals and organic pollutants)
in feral fish.
D.3.11B: Database on the biomarkers as the response to subtoxic exposure and/or effects of
selected priority pollutants
D.3.12B: Database on the time-integrated pollutants concentration (metals and organic
compounds) by means of passive samplers.
D.3.13B: Database on the groups of chemical components that are responsible for toxic
effects.
D.3.14B: Identification of "hot spots" in terms of chemical composition and potential for
toxic effects on fish.
D.3.15B: Establishment and validation of integrated effect-based tools for characterization of
river water toxicity as an integrated part of a multidisciplinary research program
D.3.16B: Final Report of impacts of pollutants on feral fish.
D.3.17B: The research papers in peer-reviewed journals.
WP 4: Integrated system for the management of the Sava River quality
D4.1: Design and installation of Web repository and DSS structure
D4.2: Web based data base on soil and sediments remediation technologies and water
treatment technologies
D4.3: Compendium on soil and sediments remediation technologies and water treatment
technologies on CD and Web
D4.4: Web based MCA decision support tool for technology assessment and selection
D4.5: Integrated software-based Decision Support System for the management of "hot spots"
in the Sava River Basin on CD and Web
D4.6: Report on the applicability of soil and water remediation technologies at the evaluation
of Sava River basin case study.
D 4.7: Report on DSS testing, verification and validation
WP 5: Social, economic and governance benefits
D.5.1: WPI framework, introduction of WFD requirements in the region.
D.5.2: Methods adjusted and ready to use in different countries along Sava River.
D.5.3.Obtained Information and results displayed for dissemination to stakeholders and
policy makers
D.5.4.: Inventory of feral fish health status.
WP 6: Dissemination
D6.1: Web server, Mailing list, Usenet News, presentations to government and
nongovernmental organizations, CD and/or DVD.
D6.2: Technical and scientific reports; Publications; Presentations.
D6.3: Dissemination of manuals of proposed methods.




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REPORT on work done:

General remarks:
In the third year of the SARIB project all partners achieved the planned goals. The main
partners‟ activities were oriented to evaluate the data obtained during the first and the second
project year, to write scientific papers and to present the work on several conferences and
meetings. Efforts were oriented to estimate the extent of pollution and identification of the
anthropogenic inputs to the Sava River and its main tributaries. Sources of dissolved
inorganic carbon (DIC) were identified as well. Based on complex investigation of the extent
of pollution of selected elements and organic pollutants in sediments of the Sava River the
ecological status of the Sava River was estimated and compared to other river basins in
Europe. “Hot spots” or locations with elevated pollutant concentrations were identified.
Biomarkers as the response to subtoxic exposure were applied in feral fish. Health status of
feral fish was estimated. Potentiometric Sensor for Determination of Low Levels of Anionic
Surfactants in Industrial Effluents was developed. By ecotoxicological evaluation of sediment
pore water the most toxic sites on the Sava River were identified. The use of micro scale
bioassays was applied to characterise the toxic potential of sediment organic extracts and pore
water from the Sava River. Integrated classification model was established for sediment
contamination and toxicity characterisation.
Geographical information system (GIS) was developed and experimental data obtained within
the SARIB project were filled into GIS database.
Experimental data obtained within the SARIB project were also used to propose sediments
remediation technologies and water treatment technologies.
To improve the quality of life and for the achievement of sustainable economic development
of the society in the Sava River Basin, socio-economic parameters related to water resources
in Sava countries through the application of “Water Poverty Index (WPI), which monitors
both, the available water resources and socio-economic parameters that influence the use of
these resources, was applied.
SARIB web page (www.sarib.net) was upgraded with pdf files of published papers. One of
the most important achievements within the SARIB project was that GIS database was linked
to the web SARIB page.
Within the third project year partners performed intensive dissemination activities through
presentations on conferences and meetings and through writing scientific papers.
The SARIB project established an interface also with EU SedNet project. The SARIB project
is now recognised in the governmental bodies of the riparian Sava River countries. As
planned, the results of the SARIB project reached the “end users”. One of the most important
“end user” of the outcomes of the SARIB project is the Sava Commission. Sava Commission
expressed an extreme interest for collaboration with SARIB partners and for the use of the
results of the SARIB project and in particular data of the GIS system and a SARIB web page.
In November 2007 the Sava Commission will get the SARIB web page available for the use
and will care for its further maintenance.
Based on the above summarised activities of the SARIB project, a co-ordinator may
with a great pleasure conclude, that the partners fulfilled all the planned goals and that
the SARIB project was extremely successful.


The work performed in the third project year is described in the following report and in
Annexes 1-11 that include the extend report of particular partners.




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The SARIB report consists of the six work-package reports. In the present report short
summary of work done by particular partners is presented.

The detail description of work done by a particular partner is presented in the
EXTENDED SARIB REPORT in enclosed ANNEXES 1-11. Annexes are numbered
with the partner’s No. as follows from the List of partners (page 4).




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Workpackage 1: Coordination
Objectives are:

Coordination of all processes occurring in the framework of the project to ensure the SARIB
to functionate smoothly throughout the duration of the project.

Description of work done by co-ordinartor, partner No. 1 JSI:

WP 1: Coordination
Within the WP 1 PARTNER No. 1 JSI performed according to deliverables
D 1.1: Yearly finance statements
D 1.2: Yearly financing plan
D 1.4: Progress reports
D 1.5: Final report

the following activities:

      Administrative day-to-day and long term management of the project by establishing
continuous contacts with partners by e-mail and telephone talks.

      Advising partners about finance planning and finance statements.

       Establishing interface with the Sava Commission on meeting in Zagreb (Croatia) in
January 2007, SARIB meeting in Ljubljana (Slovenia) in May 2007 and regular Sava
Commission meeting in Banja Luka (Bosnia and Herzegovina) in June 2007. Sava
Commission as end-user of the outcomes of the SARIB project expressed an extreme interest
for collaboration with SARIB partners and for the use of the results of the SARIB project and
in particular data of the GIS system and SARIB web page. For needs of the Sava
Commission, a document with the main achievements of the SARIB project was prepared and
transmitted to the Sava Commission (Annex 1.1.). An invited lecture of the outcomes of the
SARIB project was presented at regular Sava Commission meeting in Banja Luka in June
2007 (Annex 1.2.1., Annex 1.2.2.).

       Establishing interface with European Sediment Network – SedNet. The goals of this
network are to incorporate sediment issues and knowledge into European strategies to support
the achievement of a good environmental status and to develop new tools for sediment
management on a river basin scale. As SedNet brought together experts from science,
administration and industry, representative of the SARIB project (coordinator) was invited to
contribute in development of new tools for sediment management. In this view, the strategies
of sediment analysis were presented as a poster at SedNet confenrence in November 2006 in
Venice in Italy (Annex 1.3.1., Annex 1.3.2.) and as an invited lecture and published
proceedings contribution at SedNet conference in Lisbon in May 2007 (Annex 1.4.1., Annex
1.4.2., Annex 1.4.3.). In addition, an overview of outcomes of the sediment quality of the
Sava River was reported in SedNet newsletter in September 2007 (Annex 1.5.).

       Establishing interface with the European Commission (EC) by telephone talks with
the project officers.

      Supervision of the project progress according to the objectives and milestones.

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      Assistance in organization of regular SARIB meeting in Belgrade in October 2006.

      Organization of financial-management consultations with the Partner 6 (PUPIN) in
Ljubljana in January 2007.

      Organisation of the FINAL SARIB meeting in Ljubljana in May 2007.

      Arranging and submission of the SARIB minutes to EC.

      Preparation of third year finance statements for the SARIB project and providing
information to other partners.

      Advising partners how to estimate costs regarding income from EC.

      Preparation of the third year SARIB report and providing information to other
partners.

      Preparation of the FINAL SARIB report and providing information to other partners.

    Delivering the Financial management report, the Third year SARIB report and the
FIANAL SARIB report to EC.




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Workpackage 2: Data Base and Tools
Objectives are:
a)      To build harmonised, reliable and efficient set of data and information on the Sava
river catchment with the purpose to a) model and analyse state of the Sava river – water
chemical and ecological quality and trends, b) define »hot spots« (areas that pose risk to
environment, areas that should be studied in details), c) link water quality with pollution
sources and other pressures (such area hydro morphological, flow control..) , d) link pressures
to driving sources (sociological, economical, political, institutional and legal aspects) and to
e) support with relevant data scenario and risk assessment modelling;
b)      To develop data and information exchange and reporting system for SARIB
c) To develop DPSIR indicator system for the Sava river: proposal for best set of indicators,
evaluation of chosen indicators (past and present trends, indicators for different spatial criteria
- countries, regions, river sections)
d) To link water quality and water flow models with the information system (data input and
output)

Work in the WP 2 has been performed by partners No. 1 JSI, No. 2 UL FGG, No. 3
RBI, No. 5 HEIS, No. 6 PUPIN, No. 9 BOKU Wien and No. 10 NIVA.

CONTRIBUTION of partner No. 1 JSI to WP 2:

Within the WP 2 PARTNER No. 1 JSI performed according to deliverable
D2.3: GIS platforms development - on web and on CD
the following activities:
Data obtained in situ at the sampling site and the data on different chemical characteristics of
the samples obtained later on in the laboratory were directly entered in the database which
was built-up in the MS Access by partner No.2. First database was updated with general
information about sampling locations (e.g. latitude, longitude, altitude and country, nearest
gauging stations, remarks and observations during the sampling campaign…). Sample
description in the database includes the type and media that was sampled (sediment and/or
water, aerated or non-aerated conditions…).
The following parameters measured at the sampling site were included in the database: pH,
water temperature, conductivity and dissolved oxygen. Parameters measured in the laboratory
that were entered in the database are given in the table 1:

Zinc              PCB 31         Benzo(g,h,i)perylene      Chrysene                    Al3+
Arsenic           PCB 194        Benzo(b)fluoranthene      Aldine                      Ca2+
Cadmium           PCB 180        Benzo(k)fluoranthene      Acenaphtene                 Fe2+
Lead              PCB 170        Acenapthylene             Phenantrene                 K+
Copper            PCB 153        Lindane                   Dibenzo(a,h)antracene       Li+
Chromium          PCB 44         Fluorene                  Benzo(a)pyrene              Mg2+
Nickel            PCB 101        Fluoranthene              Benzo(a)antracene           Na+
Mercury           PCB 28         Naphtalene                Antracene                   Si2+
DOC               PCB 52         Dieldrine                 Indeno(1,2,3 cd)pyrene      Sr2+
Alkalinity        PCB 149        p,p DDD                   Heptachlor                  F-
                  PCB 138        p,p DDE                   Endrine                     Cl-
                  PCB 180        p,p DDT                   Pyrene                      NO3-
                  PCB 118        HCB                                                   SO42-



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All the parameters in the database are given together with the following attributes: measured
concentration value, CAS number and a short description of the method used during the
laboratory work. Two examples of the entering form are given in Figure 1.




                     Fig. 1: Two example of the database entering form




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CONTRIBUTION of partner No. 2 UL FGG to WP 2:

D2.1: Report on collected data and information in all countries

In the period data on geology have been obtained and put into the system. The data on
geology is missing for Croatia. In the same period we obtained data form Eionet Waterbase
data source. For the GIS two new thematic layers have been prepared: area with the highest
degree of point source and dispersed source pollution. In the period a relational data base
model has been formed in MS ACCESS environment for the collection of data on sampling
done by allpartners during SARIB project. Directions how to put data into data base have
beenprepared and send to all partners. The SARIB relational database contains: list of
sampling location and results of sampling campaighn parameters (chemical analyses,
biological parameters, hydromorphological data). Partners have send their data imported
through data base replicate they got.

D2.2 Structure of the GIS definition, data harmonisation

The final structure of GIS system is the following:

1.  RASTER IMAGE BACKGROUND
2.  ADMINISTRATIVE BORDERS BORDERS
3.  THE SAVA RIVER CATCHMENT
4.  RIVERS
   The Danube River
   The Sava River and The Sava River Tributaries
5.  WATER QUALITY
6.  CORINE LANDCOVER 2000
7.  AREAS WITH SIGNIFICANT POINT ON DIFFUSE SOURCES– AGRICULTURE
8.  AREAS WITH SIGNIFICANT POINT ON DIFFUSE SOURCES – URBAN AREAS
9.  DISTANCE FROM CONFLUENCE
10. HYDROLOGICAL STATIONS
11. METEOROLOGICAL STATIONS
12. MEASUREMENT POINTS
13. GEOLOGY

Harmonisation was done with integration of data, imported into Sarib Database and SARIB
GIS. Data on sampling and laboratory testing were put into replications, haivng inherent data
quality check and assurance. Copies of replicaitons were used independently at different
locations, and the individual replicas hase been synchronised with the „master‟ copy of the
database.

D2.3 GIS platforms development - on web and on CD

SARIB GIS platform is organised as spatially homogenised data warehouse with catalog and
data themselves. Spatial data are organised by semantic areas. Data are prepared and imported
in original form by PC market available software packages. Layers have descriptions of
sources of data (collected), and status of development (done, in preparation, planed). The
following layers are included: The Sava river catchment area, The Sava River, The Sava
River tributaries, The Danube River (part), measurement points (chem. anal.), fish sampling
points, meteorological stations (not complete), hydrological stations, CORINE (land cover)


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(not complete), simplified raster data for the Sava river riparian countries, distance from the
source, Corine land cover data and hydrological stations.

D2.5: Report on DPSIR indicators for the Sava river catchment

There were 17 locations of sediment sampling sites along the whole Sava river done and size
distribution determined. Additional 7 volumetric sampling of bed load in hydraulically active
sections/central parts of the river in Slovenia and 3 in Croatia (Jankomir, Hrušica, Lukavec)
were added and analysis of sediments performed.

From Corine land cover data two thematic GIS data have been generated, areas with pollution
from urban activities and industrial sources and areas with pollution from agriculture.

The Watbal model was 1) applied in more detail for 12 balance regions of the Sava river and
its tributaries in Slovenia with the areas between 390 and 1880 km2 and 2) applied for the
period 1961–2000. The results of the model for Slovenia were satisfactory. There were some
problems with the calibration procedure for mountainous and karstic balance regions. Trends
show decreasing 40-years trends of discharges and precipitation and increasing 40-years
trends of actual evapotranspiration in all balance regions. Those trends are inside natural
multi-year oscillation amplitudes.


CONTRIBUTION of partner No. 3 RBI to WP 2:

D.2.2. For the development of information exchange and reporting system for SARIB project,
in the third year Partner 3 (RBI) supplied the amended coded Tables to be inserted in the
programme for data storage and GIS reporting system. Specific contribution refers to the feral
fish European chub and bioavailable concentrations of metal and organic pollutants in Sava
River water at the locations of E. chub catchment. Details are available in extended report,
Annex 3.9.

CONTRIBUTION of partner No. 5 HEIS to WP 2:

According to the agreements made at SARIB Consortium Meetings (in Sarajevo, April 2006
and in Belgrade, October 2006) on establishment of relational data base in MS ACCESS
environment, aiming at integrating it into expert information system in a GIS environment,
during the third project year HEIS was working on fulfillment of the database according to
deliverables: D2.1 - Report on collected data and information in all countries; D2.3 - GIS
platforms development - on web and on CD.

HEIS was firstly collecting and systematizing necessary data, using as a main source of
information the reports of sampling champagnes and sampling analyses results obtained
during three years of project life.

Upon reviewing the Guidelines for input data into a data base, which was created by WP
leader -University of Ljubljana, Faculty of Civil and Geodetic Engineering, Slovenia (UL
FGG KSH) and provided to project partners, HEIS team entered data and information into
relational data base. (see Annex 5.1.), which included:

      list of sampling location,


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       sampling dates and number,
       sampling media,
       samples description,
       results of sampling campaign parameters (chemical analyses, biological parameters,
hydromorphological data), as well as
       information on hydrology and morphology, that is related to the longitudinal profile
(related by names, description)

CONTRIBUTION of partner No. 6 PUPIN to WP 2:

D2.4: Report on the evaluation and selection of pollution distribution modeling software

The PUPIN‟s activities on WP2 were aimed at finishing the prototype of Spatial Decision
Support System (SDSS) for sustainable land use. The prototype has been tested on an
example of industrial siting, where 15 GIS layers, related to transportation, soil conditions,
hydrology and natural and cultural heritage, were used to define the spatial constraints, while
12 non-spatial criteria (including economic costs, labor climate, living conditions and
transportation) were used to assess the municipalities according to the conditions they provide
for running a new factory. The main result of the combined spatial and multi-criteria analysis
performed by SDSS is a map of territories ranked according to their suitability for industrial
siting.

CONTRIBUTION of partner No. 9 BOKU to WP 2:

Within the WP 2 partner No. 9 BOKU according to deliverable
D2.3: GIS platforms development - on web and on CD
submitted the data for the SARIB database.


CONTRIBUTION of partner No. 10 NIVA to WP 2:

Within the WP 2 partner No. 10 NIVA according to deliverable
D2.3: GIS platforms development - on web and on CD
NIVA submitted the data for the SARIB database for compilation into an integrated database
of chemical and biological assessment of the pollution of the Sava River basin. Inclusion of
methodological description and application notes has been made to enhance the use and
comprehension of the data in future use of the database.




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Workpackage 3: Development and Validation of Specific Tools
WP3.A Pollution of sediments and water cycling processes
Objectives are:

Establish integrated tools based on a combination of chemical analysis and biological effect
methods to assess the geographical distribution and historical trends in sediment
contamination of the Sava River Basin.
The tasks that should be achieved:
1. Estimation of the extent of pollution by the identification of the most hazardous highly
mobile metal fractions and anthropogenic inputs of metals to sediments using advanced
analytical methods.
2. Chemical characterisation of persistent organic pollutants (like PCBs).
3. Assessing the total toxic potential of pollutants (metals and organic chemicals) in
sediments using biological effect methods based on micro scale bioassays;
4. Investigating water cycling processes following the sources of dissolved inorganic carbon
from the isotopic composition and the hydrology of the catchment.


Work in the WP 3A has been performed by partners No.1 JSI, No. 5 HEIS, No. 6
PUPIN, No. 9 BOKU Wien and No. 10 NIVA. Partners No. 9 and No. 10 join together
the work in WP 3A and 3B.

CONTRIBUTION of partner No. 1 JSI to WP 3A:

Within the WP 3A PARTNER No. 1 JSI performed according to deliverables
D.3.1A: Database on the spatial and temporal extent of specific pollutants in sediments of the
Sava River Basin: nitrates, phosphates, Cd, Pb, Zn, Hg, Cu, As, Cr, Ni, sixteen parent PAHs,
five methyl-PAHs, seven the most toxic PCB congeners, atrazine and desethylatrazine, butyl
and phenyl derivatives of tin.
D.3.2A: Estimation of the extent of pollution and identification of the anthropogenic inputs to
the Sava River and its main tributaries.
D.3.3A: Identification of sources of dissolved inorganic carbon (DIC).
D.3.4A: Identification of "hot spots" of pollutant load.
D.3.5A: Characterisation of the toxic potential of sediment organic extracts and pore water
from Sava River by use of micro scale bioassays
D.3.6A: Establishment of integrated classification model for sediment contamination and
toxicity characterisation
D.3.7A: Final Report on sediments.
D.3.8A: Research papers in peer-reviewed journals on sediments; Guidelines on sediment
contamination and toxicity characterisation.

the following activities:

      Elements and organic pollutants in sediments:

In the third year of the SARIB project the total phosphorous concentrations and organotin
(OTC) compounds were analyses in 20 sediments of the Sava River from its spring to its
outfall into the Danube River. In addition, the selected pesticides (HCB, Heptachlor, Aldrine,


Page 16 of 53                                                                      29/11/2011
p,p-DDE, Lindane, p,p-DDD, p,p-DDT, Diledrin and Endrine) were determined in sediments
from the Croatian, Bisnian and Serbian part of the Sava River. Since extremely high HCB
concentration was found in sediment at sampling site in Belgrade HCB(90.8 ng g-1), the
sampling of sediment was repeated in June 2007.
During the third project year the main activity within this workpackage was oriented to
evaluation of data, preparation of working reports and finally to wrote a scientific paper that
integrated the results of complex investigation of the extent of pollution of selected elements
and organic pollutants in sediments of the Sava River. The results of the scientific research on
the Sava River sediments may be gathered into the following conclusion:
Data of the analysis of sediments sampled at 20 locations along the Sava River from its spring
to its inflow into the Danube River indicate moderate elevation of Hg concentrations in
sediments (up to 0.6 mg kg-1 Hg) and Cr and Ni (up to 400 and up to 210 mg kg-1,
respectively) on industrially exposed sites. However, the latter two elements exist primarily in
sparingly soluble forms and therefore do not represent a heavy environmental burden. Pb, Zn,
Cu, Cd and As in sediments of the Sava River were in general lower than PEL values and did
not exhibit substantial pollution. P concentrations were slightly elevated (concentrations
around 1000 mg kg-1 of total P) at agricultural areas, and as a consequence of municipal
sewage discharges particularly from big cities such as Zagreb and Belgrade. Analysis of
organic pollutants indicated that the Sava River is not polluted with butyltin, phenyltin or
octyltin compounds. Among other organic pollutants PAH‟s were present in sediments of the
Sava River. Their concentrations increased downstream the Sava River. Concentrations of
PCB in sediments of the Sava River were low. From selected pesticides p,p-DDT were found
in sediments of Galdovo and Košutarica although the use of this persistent pesticide has been
banned for many years. HCB pesticide was also determined in high concentration at Šabac
and in particulary high concentration at the Belgrade sampling sites. However, the repeated
sampling showed, that this was a point source contamination.
The environmental status of sediments of the Sava River is in general comparable to other
moderately polluted rivers in Europe. As the biggest tributary to the Danube River, the Sava
River exhibits similar extent of pollution with elements. However, Cd concentrations in
sediments of the Sava River were in general lower, while Cr and Ni concentrations were in
general higher than those determined in the Danube River. According to targeted organic
analyses (organotin compounds, PAHs, PCBs and selected chlorinated pesticides) performed
within the SARIB project it may be concluded that, with exception to some sampling sites
(Ţupanja, Brĉko, Bosanska Raĉa, Gradiška and Belgrade), the pollution of the Sava River
sediments is low and generally lower than the pollution of the Danube River sediments
(ICPDR, 2002).
International Sava River Basin Commission, stake-holders, water management institutes and
local authorities of the riparian countries may use data of the present investigation as a base
for sustainable use, management and protection of the Sava River water resources.

The manuscript of the above complex research was submitted to renowned international
journal:

MILAĈIĈ Radmila, ŠĈANĈAR Janez, MURKO Simona, ZULIANI Tea, HEATH Ester,
KOCMAN David, HORVAT Milena: A complex investigation of the extent of pollution of
selected elements and persistent organic pollutants in sediments of the Sava River: Results
from the EU project SARIB, submitted to Environment International. (Annex 1.6.)

European Sediment Network – SedNet invited SARIB participants to contribute in
development of new tools for sediment management. In this view, the strategies of sediment


Page 17 of 53                                                                       29/11/2011
analysis and the summarised information about the extent of pollution of the Sava River
reported in SedNet newsletter.

MILAĈIĈ Radmila, ŠĈANĈAR Janez, HORVAT Milena, HEATH Ester, MURKO Simona,
KOCMAN David, ZULIANI Tea: Pollution of sediments of the Sava River, SedNet
newsletter, September 2007 (www.sednet.org/newsletter) (Annex 1.5.).


The data of the above research contributed also to characterisation of the toxic potential of
sediment organic extracts and pore water from Sava River by use of micro scale bioassays
and to establishment of integrated classification model for sediment contamination and
toxicity characterisation. Norwegian partners submitted a manuscript to a renowned journal.
The work is in a phase of acceptance:

KALLQVIST Torsten, MILAĈIĈ Radmila, SMITAL Tvrtko, THOMAS Kevin V., VRANES
Sanja and TOLLEFSEN Knut-Erik: Chronic toxicity of the Sava river (SE Europe) sediments
and river water to the algae Pseudokirchneriella subcapitata, submitted to Water Research
(See annex 10.5. of NIVA partner).


      Chemical and stable isotope characteristics of water in the Sava River watershed

Chemical and stable isotope approach was used to study the geochemical dynamics of carbon
and nitrogen the River Sava watershed. In the third year of the investigation much work was
performed on the evaluation of the data obtained from sampling campaign during low
discharge in fall 2005 and 2006 and high discharge in spring 2006 with the aim to capture the
hydrological extremes. The main channel of the river was sampled at 30 locations covering 11
major tributaries. We can observe changing trends from natural to direct anthropogenic
influences moving along the lower River Sava until its confluence. The results indicate that
lower Sava watershed is not influenced only by weathering processes, but also by other
effects mainly human activities. Further, biological and biochemical processes are more
dominating than in the upper flow of the Sava.
It was found that riverine mass transport of the dissolved inorganic carbon (DIC) is mainly
influenced by saturation state of carbonate minerals (calcite and dolomite) and riverine
discharge. pCO2 values are well above atmospheric equilibrium pressure and represent a
source of CO2 into the atmosphere. The theoretical calculation of the total loss of inorganic
carbon through its surface ranges from 3.18 x 107 mol/day during periods of low wind speeds
to a maximum of 3.1 x 108 mol/day at a moderate turbulence condition. These fluxes
represent between 6 and 19 % of the river‟s DIC budget. Other different contributions to the
DIC were quantified by a mass balance calculation. It appeared that dissolution of carbonates
represented between 32 and 42 % of DIC at the mouth of the River Sava at Belgrade and was
more pronounced during the fall 2006. The proportion of degradation of organic material to
DIC ranged between 20 and 23 % and was higher in spring 2006. The overall annual DIC flux
of the River Sava at Belgrade was quantified from the average discharge of 1722 m 3/s
(Republic Hydrometeorological Service of Serbia) and was estimated to be 1.9 x 1011 mol C.
Thus the River Sava represents  0.7 % of the global river carbon flux of 2.67 x 1013 mol C/
year and 23 % of the annual DIC flux of the River Danube into which River Sava flows. The
Sava dissolved organic carbon (DOC) levels were higher downstream from the “pristine”
Alpine region because agricultural activity and/or sewage discharge increases as the Sava
becomes more impacted by anthropogenic activities. The estimated DOC flux of 5.2 x 1010


Page 18 of 53                                                                      29/11/2011
mol C/ year at the Belgrade was lower comparing to the DIC annual flux. In addition the
particulate organic carbon (POC) flux was estimated to be 4.3 x 1010 mol C/ year representing
~1.1 % of the total suspended load of the River Danube into the Black Sea. As expected,
carbon fluxes indicated the dominance of DIC with 67 % over other carbon species in the
River Sava. The proportions DIC:DOC:POC of 67:18:15 are similar to the data for River
Rhone (DIC:DOC:POC = 82:6:8).
Few studies have attempted to identify sources of nitrate in surface water using stable isotopic
ratios. In most of them it was found that nitrogen isotopic composition is associated with
                                                                         15
                                                                            NNO3 > 7‰ typicaly
represent the catchments with higher percentage of agricultural and/or urban land use. On the
                                                           15
                                                              NNO3 is < 6‰. These parts are
predominantly forested watershed indicating the presence of natural soil derived nitrogen. The
statistical evaluation of the particulate organic material (POM) data including stable isotopic
composition of carbon and nitrogen showed that  18 % of the sampling sites were influenced
by plankton, 12 % by fresh terrestrial C3 plants and ~11 % by macrophyte debris. The
remaining 59 % of the samples are presumably dominated by combination of soil derived
POM with the minor contribution of other three potential sources. Therefore most of the POM
is derived from highly processed material which is largely bio-unavailable in the riverine
systems.
It was found that the average percentage of pollution of the River Sava was not changing
seasonally and was estimated to be 14.2 %, 13.2 % and 13.8 % in October 2005, May and
October 2006, respectively. On the other hand, the average percentage of pollution in
tributaries is higher reaching up to 45% in Bosna tributary in May 2006. Higher % of
pollution of 25.3% and 30.1% was also observed in Kamniška Bistrica and Savinja in October
2005, respectively. It was also found that in Croatia the River Sava is highly induced by
agricultural runoff. SEM/EDS microscopy of filters taken at Košutarica showed the presence
of the diatom alga Stephanodiscus hantzschii in both seasons. These algae are usually present
in eutrophic environments. It seems that eutrophication following a massive algal bloom was
an important process in the agricultural part of the River Sava especially during the low-flow
periods.

On this topic three papers are already published or under review and two of them are in
preparation.

KANDUĈ, Tjaša, OGRINC, Nives. Hydrogeochemical characteristics of the river Sava
watershed in Slovenia = Hidrogeokemiĉne znaĉilnosti poreĉja reke Save v Sloveniji.
Geologija 50, 157-177, 2007 (ANNEX 1.7.).
KANDUĈ, Tjaša, SZRAMEK, Kathryn, OGRINC, Nives, WALTER, Lynn M. Origin and
cycling of riverine inorganic carbon in the Sava River watershed (Slovenia) inferred from
major solutes and stable carbon isotopes. Biogeochemistry 86, 137-154, 2007 (ANNEX 1.8.).
OGRINC, Nives, MARKOVICS, Roland, KANDUĈ, Tjaša, WALTERS, M. Lynn,
HAMILTON K. Stephen, 2007. Sources and transport of carbon and nitrogen in the River
Sava watershed, a major tributary of the Danube River. Submitted to Applied Geochemistry,
2007 (ANNEX 1.9.).




Page 19 of 53                                                                       29/11/2011
CONTRIBUTION of partner No. 5 HEIS to WP 3A:

According to the agreement from the first SARIB Consortium Meeting (Ljubljana, March
2005), that HEIS will, apart from water and sediment analysis along Sava river, work on
sediment and water analysis from the four main Sava river tributaries in B&H (rivers: Una,
Vrbas, Bosna and Drina) (See Annex 5.2.Sammplin locations), during the third project year of
the project, HEIS team was working on the following in accordance to deliverables D.3.1A -
Database on the spatial and temporal extent of specific pollutants in sediments of the Sava
River Basin: Cd, Pb, Zn, Hg, Cu, As, Cr, Ni and D.3.2A: Estimation of the extent of
pollution and identification of the anthropogenic inputs to the Sava River and its main
tributaries

At the beginning of the third project year, completion of analysis of total concentration of
heavy metals in sediment and water taken from Sava river in May 2006, (locations: Srbac,
Brĉko, Raĉa) and from four main Sava river tributaries in B&H (rivers: Una, Vrbas, Bosna
and Drina), have been performed (see Annex 5.3.).
Parallel to that, the forth sampling champagne in Sava river tributaries was conducted in
October 2006 year, after a long dry period, as previous sampling champagne (May 2006 year)
was conducted after long rain period (See Annex 5.3.).

During sampling champagnes one bottle (1l) of river water and one bottle of sediment were
taken on each sampling site. Regarding sediment sampling procedure, only first 5 cm of
sediment was taken by plastic tool, together with corresponding water in 1l- PET bottle.
Samples were transported to the laboratory, where sediment was prepared for the future
analyses. Each sediment sample was processed in a way described in SARIB sapling
Protocol. According to that protocol, sediment was manually sieved to obtain 63 μm fraction,
dried on 40°C, and homogenized. Digestion was conducted in mixture of HNO3 and HClO4
(3:1) and HF, and repeated until clear solution was obtained with 3 ml 1:1 HNO 3. All
digestion was conducted in Teflon vessels, on sand bath.
Cu, Pb and Zn were analyzed by flame atomic absorption spectrometry (FAAS), while Cr,
Cd, Ni and As were analyzed by graphite furnance (electrothermal) Atomic Absorption
spectrometry (ETAAS).
With respect to method for the determination of Hg by cold vapor technique, HEIS researcher
had a 3 day visit to JSI laboratory during second project year (29th May to 1st June).
According to that, HEIS laboratory staff has performed adaptation of method for the
determination of Hg by cold vapor technique and analyzing of all samples of sediment taken
during all tree project years.

The aim of previous analysis of heavy metals in water (done during first and second project
year) was to investigate if correlation between concentration of heavy metals in sediment and
in water exists, as well as to investigate the nature of that correlation. Based on analyses of
the results from the past two years sampling champagnes in August, 2005, September 2005 it
was concluded that correlation between concentration of heavy metals in sediment and water
does not exist. Therefore analysis of heavy metals in water from forth sampling champagne in
Sava river tributaries (October, 2006) was not conducted, as it was no longer relevant for the
project. Therefore, sampled water was used only for standard physical – chemical analysis. A
part of collected samples were send to the JSI partner for further analysis, together with the
obtained results of physical – chemical analysis. Obtained results of the standard physical –
chemical analysis of water sample during third project year are presented in Annex 5.4.



Page 20 of 53                                                                      29/11/2011
According to deliverables D.3.2A - Estimation of the extent of pollution and identification
of the anthropogenic inputs to the Sava River and its main tributaries, and D.3.4 A -
Identification of "hot spots" of pollutant, after the sediment and water samples preparation
and analysis in the laboratory, HEIS performed an overall assessment of the obtained results,
comparing them among each other, comparing with the results of other project partners and
accordingly investigating at site causes of heavy metal concentration, with the purpose to:
       Investigate the influence of the weather and hydrological changes on the sediment
composition;
       Assess the pollution distribution and concentration and identify “hot-spots”;
       Create wider picture of the pollution sources of Sava river coming from BiH;
       Identify influence of B&H‟s tributaries to the Sava river quality.

Aiming to asses the pollution distribution and concentration and to identify “hot-spots”,
comparison has been performed between the obtained results and the three concentration
ranges: TEL – Threshold effect level (North America), PEL – Probable effects level (North
America), B&H – maximal allowable concentration (Max. All. Conc.) in soil according to the
law in B&H as well as investigation of pollution causes at sites (see Annex 5.5.).
The results of the above analyses showed the following:
       Concentration of Pb exceeded TEL and PEL concentration limits only in sediment
from Drina River, and according to investigation of possible cause at site it was concluded
that the mine “Sase” of Pb and Zn in Srebrenica municipality that is located in the Drina river
basin, presents the main source of pollution by Pb and Zn.
       Concentration of Cr exceeds TEL, PEL and B&H‟s Max. All. Conc. in the Bosna river
sediment. This river is most occupied river by different industries in B&H. Cause of increased
concentration of Cr in sediment from Bosna river was found in leather industry “KTK”,
“Prevent Leather” and number of small manufacture of leather processing, all in Visoko
municipality that use chemicals that containing Cr.
       Concentration of As and Hg exceeded PEL level, but TEL and B&H‟s Max. All.
Conc. were not exceeded in any case.
       Concentration of Ni slightly exceeded TEL, PEL and B&H‟s Max. All. Conc. in all
rivers, except in Bosna where Ni was found in high concentrations. The conclusion made
upon investigating situation at site was that high concentration of Ni in Bosna river originate
from the metal processing industries in Sarajevo (“Ţica” and “TDS”) and Zenica (Mittal Steel
Zenica”). Slightly increased concentration of Ni in all other BH river is likely to be natural
phenomena.

Aiming at identification of influence of B&H‟s tributaries to the Sava river quality, the results
of sediment analyses of each tributary obtained by HEIS were compared with the results of
sediment analyses of nearest up-stream and down-stream locations along Sava river, obtained
by JSI and HEIS (see Annex 5.6.).
Comparison was made between the following sampling points:
       River Una (sampling point Bosanska Gradiška, downstream from settlement
Dragsenić) -comparison was made with upstream point at Sava river in Lukavec and with
downstream sampling point at Sava river in Košutarica and Gradiška.
       River Vrbas (sampling point downstream of the municipality Srbac) - comparison was
made with upstream point at Sava river in Košutarica and Gradiška and with downstream
sampling point at Sava river in Slavonski Brod and Srbac.
       River Bosna (sampling point Municipality Orašje) - comparison was made with
upstream point at Sava river in Slavonski Brod and Srbac and with downstream sampling
point at Sava river in Brĉko and Ţupanja.


Page 21 of 53                                                                        29/11/2011
      River Drina (sampling point in Settlement Dvorovi) - comparison was made with
upstream points at Sava river in Brĉko and Ţupanja and with downstream sampling point at
Sava river in Raĉa.

The results of the above comparative analysis showed the following:
The impact of rivers Una and Vrbas on Sava river quality, in respect to heavy metal
concentration in sediment was not identified. Impact of Bosna river was identified, since
concentration of all measured heavy metals (except for Hg and Cd) in sediment was increased
downstream of estuary of Bosna river along Sava river. Significant increase occurred in
concentration of Ni, Cr and As. Impact of Drina river was also identified downstream along
Sava river, since significant increasing occurred in concentration of Pb. This was in line with
the conclusions above, i.e. confirmation of the conclusions that leather and metal industries
along Bosnia and the mine in Drina river basin presents significant “hot spots” in the Sava
river basin.

CONTRIBUTION of partner No. 6 PUPIN to WP 3A:

Accoridng to deliverable

D.3.1A: Database on the spatial and temporal extent of specific pollutants in sediments of the
Sava River Basin: nitrates, phosphates, Cd, Pb, Zn, Hg, Cu, As, Cr, Ni, sixteen parent PAHs,
five methyl-PAHs, seven the most toxic PCB congeners, atrazine and desethylatrazine, butyl
and phenyl derivatives of tin.

The activities of PUPIN were related to collecting the samples of pore water and sediments
from four sampling sites in Serbian part of the Sava River (Sremska Raĉa, Sremska
Mitrovica, Šabac and Belgrade). These samples were then analyzed by IJS, BOKU Wien and
NIVA as part of their activities on WP3.


CONTRIBUTION of partner 9 BOKU Wien to WP 3:

During the third year of SARIB project Partner No. 9 BOKU Wien participated in WP3.A
related to the ecotoxicological evaluation of Sava River Basin sediments. According to
deliverables:

       D 3.4 - Identification of "hot spots" of pollutant load,
       D 3.5 - Characterisation of the toxic potential of sediment organic extracts and pore
water from Sava River by use of micro scale bioassays,
       D 3.6 - Establishment of integrated classification model for sediment contamination
and toxicity characterisation,
       D 3.7 - Final Report on sediments,
       D 3.8 - Research papers in peer-reviewed journals on sediments; Guidelines on
sediment contamination and toxicity characterisation,
       D 3.15 - Establishment and validation of integrated effect-based tools for
characterization of river water toxicity as an integrated part of a multidisciplinary research
program and
       D 3.17 - Research papers in peer-reviewed journals

project activities during the 3rd project year focused on:


Page 22 of 53                                                                      29/11/2011
       completing the last toxicological analysis with the samples (whole sediment and pore
water) from the last sampling campaign in Bosnia & Herzegovina and Serbia
       data evaluation and comparison with a data obtained by project partner No. 1 JSI and
No. 10 NIVA.
       preparation of project report.

MAIN RESULTS
Ecotoxicological evaluation of sediment pore water identified VRHOVO as well as three
upper river locations (S.MITROVICA, SABAC and BEOGRAD) as the most toxic sites.
Whole sediment toxicity was highest to ostracods at the three initially sampled sites (MOSTE
– JEVNICA) as well as at the last four locations (B.RACA – BEOGRAD), whereas whole
sediment from LUKAVAC displayed the highest toxic effect to bacteria. Enclosed find
detailed description of performed work and obtained results in ANNEX 9 BOKU Wien.
The results have been used for the preparation of one scientific paper.

DEVIATION FROM TECHNICAL ANNEX
All planed activities of partner No. 9 BOKU Wien in WP3.A were performed according to
plan.


CONTRIBUTION of partner No. 10 NIVA to WP 3:

Partner No. 10 NIVA has in the third year performed work in WP 3A on the following areas:

Executive summary
Partner 10 – NIVA has conducted toxicity characterisation of porewater, sediments, and
surface river water from the Sava River and selected connecting tributaries through use of in
vitro bioassays and in vivo biomarker analysis. The work conducted in year three include
complementary analysis of various biological endpoints, classification of toxic potential,
submission of data for the SARIB database and evaluation of the methods used. Analysis of
vitellogenin, an estrogenic biomarker in fish, has also been coducted to complement other
analysis performed on feral fish from the Sava River. The results from these and earlier
studies has led to publication of two scientific papers in peer-review journals and two
presentations as posters on international scientific meetings.

Introduction
Partner 10 – NIVA has performed work in the SARIB project to primarily to characterize the
toxic potential in river water, sediments and sediment pore water from the Sava River and
selected connecting tributaries by using a suite of small-scale bioassays. In this work, chronic
toxicity was determined by an algae growth inhibition assay, acute toxicity and sublethal
toxicity such as arylhydrocarbon receptor (AhR) toxicity, estrogenicicty, oxidative stress and
DNA damage was determined in an in vitro fish bioassay. Inhibition of Acetylcholine
Esterase (AChE) was determined directly in anin vitro bioassay based on a purified enzyme
preparation. Partner 10 has in addition to this work provided analysis of vthe estrogenic
biomarker vitellogenin (vtg) in European Chub. The year 3 report focus on presenting the
updated results from the endpoints studies and comparing the data to identify contaminant
“hot-spots” and classification of toxicants load according to potential effects




Page 23 of 53                                                                       29/11/2011
Materials and methods
Material and methods are thoroughly described in detail in the extended reports “Multi-
endpoint toxicity screening of sediments and water samples from the Sava River” and
“Determination of plasma vitellogenin concentrations in chub (Leuciscus cephalus) from the
Sava River (SE Europe)”

Results
The results from the studies Partner 10 has been organised according to deliverables in
sections below.

Reanalysis in multi-endpoint bioassay (D. 3.5/ D. 3.15): Some reanalysis of sediments,
sediment pore water and surface river water were performed in the fish bioassays on certain
sample locations to reduce data variation. The reanalysis provided improved results, although
general conclusions were still similar to that reported in Year 2 report for partner 10. The
extended report “Multi-endpoint toxicity screening of sediments and water samples from the
Sava River”, section 3.2, annex 10) gives the details and an updated text, figures and tables
that has been used for reporting and inclusion of data into the SARIB database.

Assessment of DNA damage in extracts of sediments and river water (D. 3.5/D. 3.15):
DNA damage assessment show that extracts of water (dissolved and particulate fraction) from
some locations were causing low level of DNA damage, and DNA damage seems to be
associated with the water dissolved and particulate fraction of Sava River surface water. The
highest DNA damage was determined at Jevnica (water dissolved fraction) and Šabac
(particulate fraction). DNA damage in extracts of sediments and porewater were observed,
although thorough studies were not possible due to lack of sample materials. Details of the
results is compiled in the extended report “Multi-endpoint toxicity screening of sediments and
water samples from the Sava River”, section 3.2, Annex 10.

Neurotoxic activity in extracts of sediments and river water (D. 3.5/3.15): The neurotoxic
potential of environmetal samples from the Sava River and connecting tributaries were
assessed as inhibition of acetylcholineesterase (AChE) activity. Due to low sample volumes
available for these analysis, only sediment extracts and extracts of the water dissolved and
particulate fraction of river surface water was determined. Results from these studies show
that selected sediments and surface river water contain AChE inhibitors as well as AChE
inducers. Extracts of surface river water (Mojstrana, Moste, Jevnica, Vrhovo, Brezice and
Slavonski Brod) was the most efficient AChE inhibitors, whereas sediment samples (Jevnica
and Gradiška) caused somewhat lower effects. Induction of AChE activity was observed in
surface river water (Galdovo and Lukavec) and sediment extracts (Krupa River source, Mlin,
Vrhovo, Crnac, Lukavec, Slavonski Brod). In a similar manner, the particulate fraction of
surface river water from Moste and Galdovo caused a decrease in AChE activity, whereas the
particulate fraction of surface river water Vrhovo, Brezice, Slavonski Kobas, Slavonski Brod,
Zupanja, Sremska Mitrovica and Beograd were all causing increase in AChE activity. Details
of the results has been compiled in the extended report “Multi-endpoint toxicity screening of
sediments and water samples from the Sava River”, section 3.3, Annex 10.




Page 24 of 53                                                                     29/11/2011
Table 1. Toxicity “hot-spots” in the Sava River and connecting tributaries.

                           Algae        Fish cells

                           Growth       Cytotoxic    AhR        ER         DNA          AChE
                           inhibition   ity          agonists   agonists   damage       inhibiti
                                                                                        on
                                        Zupanja
Sediment




              Sediment     Crnac                     Mlin       Lukavec    -            Jevnica
                                        Srbac
                                        Vhrovo
              Pore water   Vrhovo                    Vrhovo     Zupanja    -            -
                                        Galdovo
                                                                           Jevnica
              Water
                                        Oborovo                            J.      na Moste
River water




              soluble      Lukavec                   Lukavec    Galdovo
                                        Galdovo                            dolen.     Vhrovo
              fraction
                                                                           Sl. Kobas
                                                     Otok
              Particle
                           Oborovo      -            Samborski -           Sabac        Galdovo
              fraction
                                                     Lukavec


Hot-spot identification and classification of the toxic potential (D. 3.6/3.14): The data
material on the toxic potential of sediment porewater, sediments and river water from the
Sava River was compiled and “hot-spots” were identified (table 1). A classification scheme of
three levels of toxic potential was also proposed on basis of the bioassay responses observed
(see “Multi-endpoint toxicity screening of sediments and water samples from the Sava River”,
section 4, Annex 10). Classification was made to discriminate between high, intermediate and
low toxicity indicated by color codes. A series of “hot spots” were identified and their toxic
potential classified as “low, intermediate or high”. The future objective is to further
harmonize the current classification to that reported data in literature and other studies
performed using similar endpoints in order to properly address environmental risk. This work
will be performed as part of publishing the data in scientific journals. Details of the “hot-spot”
identification and toxicity classification has been compiled in the extended report “Multi-
endpoint toxicity screening of sediments and water samples from the Sava River”, section 4,
Annex 10.

Scientific papers from SARIB work (D. 3.8/3.15): Results from the work has been
published in Chemosphere (reported as in press in Year 2 report), compiled into one scientific
paper submitted to Water Research and two paper under preparation to be submitted to peer-
review journals. Details are given under WP.

Analysis of European Chub plasma vitellogenin (D. 3.11): Vitellogenin (vtg), which has
been proposed to be a versatile biomarker for the exposure to estrogens, was measured in
plasma from chub (Leuciscus cephalus) caught in the Croation section of the Sava River. Vtg
levels were sampled in male fish, subjected to competitive ELISA measurements and results
compared to seasonal as well as spatial factors. Although clear significant effect were not
observed due to low sample numbers, high individual variation and lack of quantification of
low concentrations of male Vtg, trends suggest that endocrine disruption may occur during
the spring, where male fish seems to be more susceptible to exogenous estrogens than other
parts of the year. Further analysis of plasma concentrations of steroids, proposed in the project
description, was abandoned due to low volumes of plasma available from E. Chub. Details of


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the vtg a measurement has been compiled in the extended report “Determination of plasma
vitellogenin concentrations in chub (Leuciscus cephalus) from the Sava River (SE Europe)”,
Annex 10).

Submission of data to the SARIB database (D. 3.13/3.17/4.5): All data produced in this
project has been submitted to the SARIB database for compilation into an integrated database
of chemical and biological assessment of the pollution of the Sava River basin. Inclusion of
methodological description and application notes has been made to enhance the use and
comprehension of the data in future use of the database. Groups of compounds identified to
contribute to the toxicity of Sava River toxicity has been compiled into one scientific paper
that has been published in Chemosphere (Grung et al. 2007, Annex 10). Unclear links
between chemical composition and bioassay responses in different samples has made
inclusion of such data into the SARIB database difficult and thus not pursued.

Establishement and evaluation of methods (D. 3.15): A battery of bioassays based on
algae, fish cells and purified enzymes have been established for use in river water toxicity
testing and recently complemented with the analysis of DNA damage and Acetylcholine
esterase inhibition. A brief evaluation of the choice of methods and results obtained has been
performed in the extended report “Multi-endpoint toxicity screening of sediments and water
samples from the Sava River” (Annex 10) and additional evaluation will be performed as part
of scientific papers subjected to publishing in international journals.




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Workpackage 3: Development and Validation of Specific Tools
WP3.B Availability and impact of pollutants and biota
Objectives are:

Establish integrated tools based on a combination of chemical analysis and biological effect
methods to assess the presence of bioavailable pollutants in water, their potential for effect
and identifying effects on feral fish in the Sava River water.

Work in the WP 3B has been performed by partners No. 3 RBI, No. 4 PBF and No. 9
BOKU Wien and No. 10 NIVA. Partners No. 9 and No. 10 gave joint report on WP 3
activities reported in WP 3A.


CONTRIBUTION of partner No. 3 RBI to WP 3B:

Acceptable sampling technique according to the standard HRN EN 14011:2005, Water
quality-Sampling of fish with electricity has been further on applied for the feral fish
(European chub). Final fish catchment occured in September 2006 at 5 selected sites from
Otok Samoborski to Jasenovac.

D 3.9 Database on the incidence of diseased feral fish in Sava River study area
Database on the health status of European chub from the Sava River covers the last fish
sampling in September 2006, when 59 fish secimens were collected at 5 sites from Otok
Samoborski to Jasenovac. Results and the interpretation are available in Annex 3.3.

D 3.10 Database on the level of specific priority pollutants (metals and organic
pollutants) in feral fish.
Database on metal concentrations (Zn,Cu,Cd, Fe, Mn) in individual liver and gill cytosol
(S50) samples of E. chub collected in spring of 2006 and in September 2006 exists.
Additional metal analysis were performed in heat-treated cytosol (HT S50), that contains
metallothionein. For details see Annex 3.4.

Reported are the data on total PCBs, sum of 7 PCBs and total DDTs determined in the
composite samples of fish liver and muscle collected in September 2006 at defined sampling
sites on Sava River. For details see Annex 3.6.

Reported are the data on polycyclic aromatic hydrocarbons (PAHs) in European chub
composite sample of muscle and liver, collected during spring 2006 and in September 2006.
Accomplioshed is the analysis of hydroxylated metabolites of polycyclic aromatic
hydrocarbons (OH-PAHs) in European chub individual bile samples. The details are available
in Annex 3.7.

D 3.11 Database on the biomarkers as the response to subtoxic exposure and/or effects
of selected priority pollutants.
Biomarker and effect endpoints of organic pollutants (EROD activity, AMES test) in fish
liver as detoxification organ were determined in individual specimens of E. chub, collected in
September 2006 at 5 sites on Sava River. Detailed report in Annex 3.5.



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Database on biomarker of subtoxic metal exposure, metallothionein, in heat-treated cytosol of
fish liver and gills, determined in individual specimens collected in spring of 2006 and in
September 2006 exists. Total cytosolic proteins in S50 fraction of fish liver and gills were
determined, too. Detailes are available in Annex 3.4.

To Partner 10, Partner 3 shipped blood plasma samples of male E. chub collected in spring
2006 and in September 2006 at 6, respectively 5 Sava River sites, for subsequent analysis of
estrogenic biomarker vitellogenin by means of a validated ELISA method. Results will be
reported by Partner 10.

D 3.12 Database on the time-integrated pollutants concentration (metals and organic
compounds) by means of passive samplers
The results of deployment of “passive samplers” for metals (DGT) and organic pollutants
(SPMDs) on Sava River locations from Otok Samoborski to Lukavec Posavski are reported
for variable deployment duration. Detailed report in available in Annexes 3.4. (for metals)
and 3.6., 3.7. for organic pollutants.

D 3.14 Identification of "hot spots" in terms of chemical composition effects on fish
Different parameters discussed in Annexes 3.3., 3.4., 3.5., 3.6., 3.7., indicate that in general
the sites downstream of Zagreb, i.e. Oborovo, Lukavec Posavski and Jasenovac could be
classified as those where anthropogenic impact is observable.

D 3.16 Final report of impacts of pollutants on feral fish
Final contribution of Partner 3 (RBI) to the report on impact of pollutants to feral fish
European chub from Sava River is presented in the separate Annex Partner 3 Final Report.

D 3.17 The research papers in peer-reviewed journals
In the third year of SARIB project Partner 3 (RBI) accomplished the publication of 5 research
papers related to the pollutant levels (metals, PAHs, POPs) in feral fish tissues and the
exposure effects, and 2 defended Ph.D. Thesis.


CONTRIBUTION of partner No. 4 PBF to WP 3B:

According to deliverables:
D.3.15B: Establishment and validation of integrated effect-based tools for characterization of
river water toxicity as an integrated part of a multidisciplinary research program
D.3.17B: The research papers in peer-reviewed journals.

The following work was planned for the third year:
The objective of the work was determination of low concentration level of surfactants in
effluents, development of officially recommended MBAS extraction/spectrophotometric
method, determination of surfactants in samples of Sava river and comparison with of
officially recommended MBAS extraction/spectrophotometric method, development of
classical (using Hg-EDTA complex) trace metal ion determination and literature search for no
mercury electrochemical sensors for trace metal ion determination.

Abstract of the work done:
After performed determination on the modelled effluents formulations, the real industrial
effluents - on Sava river samples were carried out. The sensors mentioned served as end-point


Page 28 of 53                                                                          29/11/2011
detector by potentiometric titration of low surfactant concentration levels (down to 10-5 mol
dm-3 for anionic surfactants and 10-6 mol dm-3 for nonionic surfactants). No special sample
pretreatment was needed, except filtration when necessary. Equivalence point by noisy and
flat titration curves was determined using extended least-squares regression formalism of the
Savitzky-Golay method. The results for anionic surfactants agree satisfactory with standard
extraction-spectrophotometric MBAS method and are comparable with the results obtained
using a commercially available surfactant electrode. The accuracy and precision of the
nonionic surfactants determination have been evaluated by using standard addition method.

The following papers were published in peer-reviewed journals.

1. M. Sak-Bosnar, R. Matešić-Puaĉ, Z. Grabarić, New Potentiometric Sensor for
Determination of Low Levels of Anionic Surfactants in Industrial Effluents, Tenside
Surfactants Detergents, 2 (2006) 82-87.
2. M. Sak-Bosnar, D. Madunić-Ĉaĉić, R. Matešić-Puaĉ, Z. Grabarić, Nonionic surfactant-
selective electrode and its application for determination in real solutions, Anal. Chim. Acta,
581 (2006) 355-363.
3. M. Sak-Bosnar, D. Madunić-Ĉaĉić, R. Matešić-Puaĉ, Z. Grabarić, Sensitive
Potentiometric Method for Determination of Micromolar Level of Polyethoxylated Nonionic
Surfactants in Effluents, Tenside Surfactants Detergents, 44 (2007) 11-18.

Deviation from the third year plan:
The planned activities were done in the third year of the SARIB project.




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Workpackage 4: Integrated system for the management of the
Sava River quality
Objectives are:
The generic objective of this work package is to provide local authorities, governments,
stakeholders, environmentalists, decision-makers, technologists, experts and site owners in
the Sava River Basin countries with an integrated decision support tool for the sustainable
management of the river basin resources.

Work in the WP 4 has been performed by partners No. 1 JSI, No. 4 PBF, No. 5 HEIS,
No. 6 PUPIN, No. 8 ICS UNIDO, No. 9 BOKU Wien and No. 10 NIVA.


CONTRIBUTION of partner No. 1 JSI to WP 4:

Accoriding to deliverable:
D4.6: Report on the applicability of soil and water remediation technologies at the evaluation
of Sava River basin case study.
The partner No.1 JSI submitted all experimental data on the extent of pollution with elements
and organic pollutants of the Sava River sediments and pollution of the Sava River water to
partners No. 6 PUPIN and No. 8 ICS-UNIDO. Based on these experimental results, partners
No. 6 PUPIN and No. 8 ICS-UNIDO proposed the remediation activities.


CONTRIBUTION of partner No. 4 PBF to WP 4:

WP4: Integrated system for the management of the Sava River quality

Accoriding to deliverable:
D4.6: Report on the applicability of soil and water remediation technologies at the evaluation
of Sava River basin case study.

The following work was planned for the third year:
Mixed microbial culture preparation and application as well as sampling procedure on
wastewater inflow into Sava River are planned.

Abstract of the work done:
Chemical, physical and microbiological analyses on the collected samples have been
performed. In laboratory condition biotests on wastewater, of different industrial origin
(brewery, dairy, yeast and alcohol production industry, pharmaceutical and municipal), have
been performed as well. Mixed microbial culture for biological wastewater treatment, have
been prepared in order to be applied on pilot scale treatment procedure. Development and
application of wastewater treatment process (biological process with bioaugmentation and
mixed microbial biomass in form of granula). This includes biodegradability of toxic
compounds in industrial wastewaters, production and application of granulated mixed
microbial cultures. Within WP4, sampling procedure on wastewater inflow into Sava River
has been performed.

Deviation from the third year plan:
The planned activities were done in the third year of the SARIB project.


Page 30 of 53                                                                      29/11/2011
CONTRIBUTION of partner No. 5 HEIS to WP 4:

During the third project year HEIS team was working on a case study aiming at selection of
the most adequate and applicable soil remediation technology under real conditions, for the
proper management of existing "hot spot" in the Sava River Basin, by using software-based
decision support tool created by leader of the WP4 - Mihajlo Pupin Institute, Belgrade, as a
contribution to the Deliverable D.4.5. - Integrated software-based Decision Support System
for the management of "hot spots" in the Sava River.
HEIS team has performed the evaluation of candidate sites (Zenica and Visoko metal
industries dump sites, Vareš and Srebrenica mine dump sites, and dump site of Thermo -
electrical plant in Tuzla), located in the Sava river basin at the B&H territory. After the
evaluation of candidate sites, a dismissed coal ash dump site (from thermo-electrical power
plant) has been selected. The site is located in Bosna river basin, near town of Tuzla (B&H).

The selected site has the characteristics of a “hot spot” because of its potential to contaminate
soil and surface and groundwater with arsenic, boron, heavy metals, etc. In addition, under a
growing surrounding anthrpogenic pressure, there is an increasing demand to reclame the
contaminated land for other purposes. Thermal Power Plant Tuzla (TPPT) in Bosnia and
Herzegovina has, during many years of work, deposited over 40 million m3 of ash and slag at
four localities in its immediate surroundings. It is estimated that, on average, around 800 000
m3 of this material is deposited on the annual basis. All four disposal sites of the TPPT
represent, in terms of ecology, very risky area. The impact of these disposal sites on waters,
and transfer of these impacts via waters to the wider surroundings, also represents the most
direct impact on the animal and plant world, or directly on the people living in the immediate
contact with and using the surface and ground waters (see Annex 5.7.).

After site selection, HEIS team has worked on data collection in order to define site
characteristics in terms of contaminants and expected remediation needs. Basic geometrical
data (surface, volumes, etc.), contaminants level and type (heavy metals) and environmental
impact (polluted leacheate stream and potential negative effects on underground water) have
also been assessed (see Annex 5.7.). Thereafter, priority technological and economical criteria
applicable to the site situation have been assessed. As the main contaminants of the site are
heavy metals (As, B, Cd, Cu, Mn, Ni, Pb and Zn), technologies concerning heavy metals
removal or stabilization have been deemed a top priority in the selection process. Beside
technology affinity with heavy metal remediation, other 5 criteria have been selected to define
constrains to the analysis: Overall Cost, Reliability and Maintenance, Development Status,
Stand Alone Character, Residuals Produced. For each one of the criteria an adequate weight
factor has been selected and discussed (see Annex 5.7.).
The final query to the multi-criteria software was made including all criteria, their ratings
(maximized or minimized) and their waiting factors (see Annex 5.7.).

As the final output, the software-based multi-criteria analyses produced top three processes:

   Rank                         Name                   Input Flow     Output Flow      Net Flow
    1           Solidification/Stabilization (In Situ)    0,91           13,6           12,69
    2           Solidification/Stabilization (Ex Situ)    3,21           12,2           08,99
    3            Landfill Cap And Enhancements            2,69           11,3           08,58



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The multi-criteria software-based analysis was proven as efficient tool for ranking of soil
remediation technologies for the study site. Upon gaining the results of the software-based
Decision Support System for the management of "hot spots", the additional expert-technical
evaluation was needed. Consequently, between the three top ranked processes, after technical
evaluation, the Landfill Cap and Enhancements was shown as the best remediation
technology for the coal ash disposal site in Tuzla (B&H), which could the most effectively
solve the problem with low overall costs, simplicity and reliability (see Annex 5.7.).


CONTRIBUTION of partner No. 6 PUPIN to WP 4:

PUPIN has worked closely with ICS UNIDO on the activities related to WP4:

Implementation of Web-based Decision Support System for remediation technology
assessment and selection (D 4.4 Web based MCA decision support tool for technology
assessment and selection: see Annex 6, Section 2)
       Implementation of integrated DSS for the management of “hot spots” in the Sava
River Basin (D 4.5 Integrated software-based Decision Support System for the
management of "hot spots" in the Sava River Basin on CD and Web: see Annex 6,
Section 3)
       Testing the applicability of soil and water remediation technologies at the evaluation
of Sava River basin case study (D 4.6 Report on the applicability of soil and water
remediation technologies at the evaluation of Sava River basin case study, see Annex 6,
Section 4)
       Testing, verification and validation of DSS for remediation technology assessment and
selection (D 4.7 Report on DSS testing, verification and validation, see Annex 6, Section
5)

Based on the compendium of remediation technologies (37 groundwater treatment and 37 soil
remediation technologies) provided by ICS UNIDO and HEIS, PUPIN has developed a Web-
based decision support system (DSS) for technology assessment and selection. This DSS is
implemented as an Internet/Intranet software aimed at various stakeholders including
environmentalists, regulators, policy makers, investors, etc., to select the best available
remediation technologies for each hot spot detected in the Sava River Basin according to the
applicability of each technology for the spotted contaminants (e.g. heavy metals, X-SVOCs,
SVOCs, explosives, fuels, inorganics, radionuclides, etc.) and in accordance to his/her
preferences related to various technological, economic, social and environmental criteria (e.g.
development status, overall costs, public acceptability, residuals produced, etc.).

Many spatial data about Sava River Basin including the sampling locations are presented by
the SARIB GIS, so it would be convenient if the main functionality of the DSS for technology
assessment and selection would be accessible via GIS. Therefore, PUPIN has integrated the
DSS for “hot spot” management with GIS and provided the functionality for selecting
contaminant groups, technological, economic, social and environmental criteria, for defining
their importance, for choosing the remediation technologies and for performing Multi-Criteria
Analysis to assess and select the best technologies.

ICS UNIDO has provided a compendium with the remediation technologies, main groups of
contaminants that can be treated by these technologies and the corresponding set of
technological, economic, social and environmental criteria that can be used for the assessment


Page 32 of 53                                                                      29/11/2011
and selection of these technologies. On the other hand IJS has provided a list of contaminants
that have been detected (and the contamination extent measured) along the Sava River. With
the help of ICS UNIDO PUPIN has matched the list of detected contaminants with the
corresponding groups of contaminants to check if the technologies from the compendium can
be used for the treatment of these contaminants. The applicability of remediation technologies
has been tested on various sites along Sava River using real data about detected contaminants.

The DSS for technology assessment and selection has been tested, verified and validated
following the standards prescribed by software engineering. Unit testing has been applied on
all Java classes and their methods, integration testing has been performed on all interfaces
between various DSS components, system testing has been performed to check the
functionality of the overall system, DSS has been verified to check if the implementation of
the system conforms to the design, and finally the system has been validated to check if it can
be applied on various hot spots along the Sava River.

CONTRIBUTION of partner No. 8 ICS-UNIDO to WP 4:

Within the WP 4 (Integrated system for the management of the Sava river quality), in the
period 01/08/2006 to 31/07/2007 ICS-UNIDO has finalized, in close collaboration with all
involved partners and in particular with PUPIN (Mihajlo Pupin Institute), a comprehensive
database on soil, sediments and groundwater remediation technologies and their applications,
which has been utilized for the development of Web repository of remediation technologies
and the development of Decision Support System (DSS) for remediation technology
assessment and selection.
Additional parts regarding specific technologies for sediments‟ treatment and dredging
operations have been prepared. According to the specific environmental characteristics of the
Sava basin and its related contamination problems/issues, as resulted trough the latest findings
and assessments within SARIB project, further criteria for the evaluation of technological
applicability and technology performance have been identified and developed. In particular, a
new contaminant classification has been developed and remediation technologies
performances have been assessed and ranked accordingly.
ICS-UNIDO has also assisted in the finalization of GIS Database and DSS tools in
collaboration with the University of Ljubljana, Faculty of Civil and Geodetic Engineering and
Mihajlo Pupin Institute for providing a Spatial Decision Support System (SDSS), which is
suitable for sustainable land management.

During the project‟s third year ICS-UNIDO has worked on the planned activities of WP4 and
finalized all relevant deliverables, as listed below:
           D4.2: Web based data base on soil and sediments remediation technologies and
water treatment technologies.
Within this tasks, ICS UNIDO has finalized the database on soil, sediments and groundwater
remediation technologies (35 soil, 24 sediment, 20 groundwater clean-up technologies, 6 off
gas treatments), evaluating the main groups of contaminants that can be treated by these
technologies and defining the corresponding set of technological, economic, social and
environmental criteria that can be used for their assessment and selection. An additional part
regarding dredging operations (6 dredging techniques) has been also prepared. All materials
have been provided to Mihajlo Pupin Institute for their inclusion in the SARIB Web
repository (for the relevant deliverable, please refer to Mihajlo Pupin Institute reports for
SARIB).



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          D4.3: Compendium on soil and sediments remediation technologies and water
treatment technologies on CD and Web.
Within this task, ICS-UNIDO has prepared and finalized the compendium, which is enclosed
in Annex D4.3 of the SARIB third year report.

ICS-UNIDO has also worked closely with Mihajlo Pupin Institute on the activities related to
WP4, i.e.:
           Implementation of Web-based Decision Support System for remediation
technology assessment and selection
           Implementation of integrated DSS for the management of “hot spots” in the Sava
River Basin Testing the applicability of soil and water remediation technologies at the
evaluation of Sava River basin case study
           Testing, verification and validation of DSS for remediation technology assessment
and selection

Within these tasks, ICS UNIDO and Mihajlo Pupin Institute have matched the list of detected
contaminants provided by IJS with the corresponding contaminants classes defined in the
compendium to check if the technologies included can be used for their treatment. The
applicability of remediation technologies has been tested on various sites along Sava River
using real data about detected contaminants. Based on the compendium of remediation
technologies provided by ICS UNIDO and HEIS, a Web-based decision support system
(DSS) for technology assessment and selection has been developed by Mihajlo Pupin
Institute. This DSS is implemented as an Internet/Intranet software aimed at various
stakeholders including environmentalists, regulators, policy makers, investors, etc., to select
the best available remediation technologies for each hot spot detected in the Sava River Basin
according to the applicability of each technology for the spotted contaminants (e.g. heavy
metals, X-SVOCs, SVOCs, explosives, fuels, inorganics, radionuclides, etc.) and in
accordance to his/her preferences related to various technological, economic, social and
environmental criteria (e.g. development status, overall costs, public acceptability, residuals
produced, etc.). For the relevant deliverable, please refer to Mihajlo Pupin Institute reports for
SARIB.


CONTRIBUTION of partner No. 9 BOKU to WP4:

According to deliverables:
       D 4.5 - Integrated software-based Decision Support System for the management of
"hot spots" in the Sava River Basin on CD and Web, and
       D 4.6 - Report on the applicability of soil and water remediation technologies at the
evaluation of Sava River basin case study
project activities during the 3rd project year focused on identification of toxicological “hot
spots” along the Sava River Basin.

Contribution of Partner No. 9 BOKU Wien is summarised in follow items:

       Identification of toxicological “hot spots” along the Sava River Basin using different
toxic tests selected during the first project year.
       Special care was taken in order to select the most appropriate tests for the Sava River
Basin. In order to fulfil this criterion optimisation and adaptation of tests for the use in the
region of interest was the objective.


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       Selected tests are fitting the criteria for the ecotoxicological evaluation of the river
Sava samples, especially for the determination of “hot spots” and should be used for the
further monitoring of the Sava river quality.

Deviation from the technical annex:
All planed activities of partner No. 9 BOKU Wien in WP4 were

CONTRIBUTION of partner No. 10 NIVA to WP4:

According to deliverable
D 4.5 - Integrated software-based Decision Support System for the management of "hot
spots" in the Sava River Basin on CD and Web
Submission of data to the SARIB database was performed (D. 3.13/3.17/4.5): All data
produced in this project has been submitted to the SARIB database for compilation into an
integrated database of chemical and biological assessment of the pollution of the Sava River
basin. Inclusion of methodological description and application notes has been made to
enhance the use and comprehension of the data in future use of the database. Groups of
compounds identified to contribute to the toxicity of Sava River toxicity has been compiled
into one scientific paper that has been published in Chemosphere (Grung et al. 2007, Annex
10). Unclear links between chemical composition and bioassay responses in different samples
has made inclusion of such data into the SARIB database difficult and thus not pursued.




Page 35 of 53                                                                       29/11/2011
    Workpackage 5: Social, economic and governance benefits
    Objectives are:
    The objective is to consequently increase the quality of life and achieve sustainable economic
    development of the society in the Sava River Basin. Data and information obtained will be
    adjusted to users of water resources and level of country economy throughout the Sava River
    Basin. For that cause-effect, affordability and pressure impact have to be studied in particular
    situation for every country involved related to use of water resources Remedial measures
    proposed have to be compared to the environment improvement. The objective of this WP is
    to make an introduction of socio-economic aspects of Water Framework Directive (WFD)
    requirements in the region of SRB, based on parameters under investigation.

    Work in the WP 5 has been performed by partners No. 5 HEIS, No. 6 PUPIN, No. 7
    FABL and No. 11 IMOS GEATEH.


    CONTRIBUTION of partner No. 5 HEIS to WP 5:

    Upon testing of the WPI method by calculating WPI at “national level” for Slovenia, Bosnia and
    Herzegovina and Serbia, and upon testing of the WPI method at “river basin level” during the second
    project year, an evaluation of the WPI method was made by Partners at the Project Consortium
    Meeting held in Sarajevo (April 2006). The objective of this evaluation was to determine if the test
    method, which has been used, was adequate for usage in Sava riparian countries. Based on this
    evaluation and its conclusions, it was decided that some adjustments of the method are necessary
    regarding the sub-components of the original WPI method. Consequently, during the third project
    year, HEIS team was working on this task, in accordance with D.5.2. Methods adjusted and ready to
    use in different countries along Sava River.

    As some of the WPI sub-components were defined as irrelevant for the Sava countries, such as under-
    5 mortality rate and percentage of the country under severe water stress, their exclusion from the WPI
    method for Sava riparian countries was proposed. Namely, severe water stress is normally linked with
    droughts and severe water scarcity which is not typical for Sava river basin. Perhaps it could occur on
    some locations of the basin, but is not a characteristic of the whole river basin. Furthermore, water
    stress could be related to increased child mortality rates, where insufficient water quantity and poor
    water quality might be linked with child diseases leading to death. It was also concluded that this is
    not representative for Sava river basin, and therefore, both of these indicators were excluded as non-
    typical for Sava riparian countries.

    Also, project partners were suggesting that the original method which was tested could be improved
    with some new indicators, particularly those relevant for environment component of the Index. It was
    found that this component was insufficiently covered in the tested original method. Project partners
    recommended that the original method should be amended, so it can be more representative and
    relevant for Sava riparian countries. Based on those recommendations, HEIS team has made a detailed
    analysis of the existing internationally used indicators for water and environment sector, in order to
    select those indicators which would be included in the WPI method for Sava riparian countries. For
    that purpose EEA set of indicators and Mediterranean set of Sustainable Development of Indicators
    (MSDI) were considered.

    After research of the available indicators focused on environment and water, from the above
    mentioned sources, HEIS has narrowed the list of potential indicators that might be relevant and
    significant for WPI method for Sava riparian countries. After detailed analysis and after consultations
    between partners, the final list came down to four indicators, as follows:
            Share of population connected to wastewater treatment,


    Page 36 of 53                                                                             29/11/2011
           Share of industrial wastewater treated on site,
           Public expenditure on environmental protection as a percentage of GDP,
           Share of population served by sanitary landfills.

    These indicators are described in detail in Annex 5.8., in terms of their definition, purpose, formula for
    calculation and unit.

    After this work was concluded, the new WPI method adjusted for the application in Sava riparian
    countries was defined (see Annex 5.9.), which corresponds to Deliverable D.5.2. – Methods adjusted
    and ready to use in different countries along Sava River.

    After the new method was designed, HEIS team has conducted calculation of WPI for Bosnia and
    Herzegovina. HEIS has also worked on the research of referent values (goal points), which is
    necessary for calculation of relative value of the new indicators, but very little data was found
    available about the new indicators for other countries worldwide. Referent values for only two of four
    new indicators was found available. The research of referent values was based on internet research of
    relevant projects and documents. Finally, HEIS team has calculated WPI for Bosnia and Herzegovina
    applying the new WPI method for “national level” (see Annex 5.10.).

    Proposal of the new sub-components for the WPI method was presented at the Project Consortium
    Meeting in Belgrade (October 2006), where no further remarks or suggestions were made by other
    project partners. Therefore, the new concept of WPI method at “national level” for Sava riparian
    countries was accepted, according to Deliverable D.5.2. - Methods adjusted and ready to use in
    different countries along Sava River.

    During the mentioned Consortium meeting in Belgrade, project partners have discussed about the
    possibility of changing the name of the Index, since Water Poverty Index does not reflect its main
    purpose for Sava river basin, unlike e.g. some basins in Africa. Consequently, at the last Consortium
    Meeting held in Ljubljana (May 2007) it was agreed that more appropriate title of the Index for Sava
    countries would be Water Use Efficiency Index.

    According to Deliverable D.5.3 – Obtained information and results displayed for dissemination to
    stakeholders and policy makers, during the third year of the project, the obtained results from the
    development and testing of WPI method for Sava riparian countries were displayed for dissemination
    to the stakeholders and policy makers, namely to the representatives of: environmental NGOs, relevant
    public institutions and government administration, as well as to Sava Commission members.
    One of the occasions, where WPI method was disseminated, was the conference organized by
    Sarajevo Rectorat, where representatives of the following institutions were present: Public Enterprise
    for Sava Watershed, Federal Ministry for Agriculture, Water Management and Forestry, Federal
    Ministry for Environment and Tourism, Faculty for Civil Engineering, Medical Faculty, etc.
    (Sarajevo, February 2007). (see Annexes 5.12)
    The second occasion was a round table (Zagreb, November 2006) where representatives of Sava
    Commission were present, as well as representative of many other relevant institutions from Slovenia,
    Croatia, Bosnia and Herzegovina and Serbia (see Annexes 5.11.).


    CONTRIBUTION of partner No. 6 PUPIN to WP 5:

    According to deliverable

    D.5.1: WPI framework, introduction of WFD requirements in the region.

    The activities of PUPIN on WP5 were aimed at collecting the national indicators and
    statistical data for Serbia, which have been used then by HEIS to introduce the socio-


    Page 37 of 53                                                                                29/11/2011
    economic aspects of Water Framework Directive requirements in the Sava River Basin and to
    develop specific tool-instrument (e.g. Water Poverty Index) to monitor both the available
    water resources and socio-economic determinants which influence use of these resources.

    CONTRIBUTION of partner No. 7 FABL to WP 5:

    During the third year of SARIB project Partner No. 7 FABL participated in WP5 and has
    completed the following activities related to the: analysis of socio-economic indicators in
    riparian countries in regard to water uses, waste water quantity and industrial/agricultural
    emissions; collection of data needed by surveys and questioners and selection of pilot regions
    for research, according to development scenarios.
    According to deliverable:

           D 5.3 – Obtained information and results for dissemination to stakeholders and policy
    makers,
    project activities during the 3rd project year were focused on:
    selection of pilot regions for research, according to development scenarios in Bosnia and
    Herzegovina.

    MAIN RESULTS:
    Bardaca - Wetland of International Importance (designated on 2nd February 2007 as a Ramsar
    site No. 1658) is very suitable area for the future pilot research projects.
    The results have been used for dissemination to stakeholders as well as to the policy makers.

    The planned activities were done in the third year of the SARIB project.


    CONTRIBUTION of partner No. 11 IMOS GEATEH to WP 5:

    In the scope of WP 5, and according to deliverables:

    D.5.1: WPI framework, introduction of WFD requirements in the region.
    D.5.2: Methods adjusted and ready to use in different countries along Sava River.
    D.5.3.Obtained Information and results displayed for dissemination to stakeholders and
    policy makers
    D.5.4.: Inventory of feral fish health status.

    Partner No. 11, Imos Geateh accomplished following activities:

               Inputs prepared for the needs of the Sava Power Utility;
    In regard to Water Framework Directive:
    POLLUTION HOT SPOTS as found from SARIB: main pressures defined (mining in BiH,
    sewage water in Serbia, agricultural use – erosion in the catchment)
    In regard to Sava River management (Sava Commission): WATER USE:
    DRINKING WATER: aquifer near Zagreb
    Application of the lessons learned from Sarib to the planned reservoir HPP Breţice in regard
    to sedimentation
    Use of contacts made for the facilitation of transboudary impacts of HPP to the Croatian
    Authorities



    Page 38 of 53                                                                     29/11/2011
    NAVIGATION: sediment transport; in regard to that problem some practical inputs have been
    passed over to utility. Main being metod of the sediment transportation monitoring


               Adjustment of the tools and methods applied for riparian countries involved in
    regard to their differences;

    The opportunity of involving SME in the project has been evaluated from the point of view of
    back-upping the scientific team with practical end users aspects. The contribution of IGT had
    to do with water pollution issues and hydropower planning at the Sava River.

    Consequently, IGT has demonstrated overview and insight in depth of practical problems,
    which should always reflect scientific research. IGT has been taking part in a team which
    provided inputs in checking approaches, methods and results from the stand point of the
    resource end user.

               Contribution to the integrated database regarding: organisation, structure, quality
    and significance of data;
        - Digitising/vectorising river network of Croatia from map 1:5000000
    - Importing spatial data from all the riparian countries into ARCGIS software editing spatial
    data
            - Separation of spatial data form countries by thematic layers and integration
            - thematic data into the Sava River catchment level (editing, cleaning, edging..)

               Cost-benefit analysis of measured parameters.
    The comparison between cost of the parameters obtained and results enabling analysis is of
    the utmost importance for settling common monitoring system in the catchment of the Sava
    River. This activity has been performed as an expertise, though proper analysis of the
    financial consequences should take place before enacting monitoring in the river basin.




    Page 39 of 53                                                                      29/11/2011
Workpackage 6: Dissemination
Objectives are:

To promote the ideas of the SARIB project among beneficiary institutions, stakeholders and
individuals in Sava River Basin countries, i.e. Slovenia, Croatia, Bosnia and Herzegovina,
Serbia and Montenegro. The idea is to assess the potential of strict research results in regard
to sediments (particulate) and biota for future management plan of the Sava River Basin in the
frame of Water Framework Directive and ICPDR (Danube Convention). It is expected that by
promoting ideas through dissemination of results the cooperation in the region (catchment)
will be re-established. By completing this project initiative toward Sava River Basin
management plan will be launched in a situation where application and research know-how
could be merged for common benefit. Scientific part of the project has been focused on
ecotoxicological investigation of biota and sediment and application of the method developed
for the assessment of environmental and health risk to the users and the public.

Work in the WP 6 has been performed by partners No. 1 JSI, No. 2 UL FGG, No. 3 RBI,
No. 4 PBF, No. 5 HEIS, No. 6 PUPIN, No. 7 FABL, No. 8 ICS UNIDO, No. 9 BOKU
Wien and No. 10 NIVA.

Partners reported the work on meetings, conferences and contributed articles in national and
international journals. These activities resulted in 41 dissemination contributions of the
SARIB partners during the third project year, that are listed at the end of this report.

As a result of collaboration of ALL partners that contributed the data into the SARIB partners
No. 2 UL FGG and No. 4 PBF LINKED GIS to the SARIB web page, what is one of the
most important dissemination activities of the SARIB project.
On the SARIB web page a list of published papers in pdf files was also put that also
contributed to the dissemination activities.
Partners disseminate the results of the SARIB project to local authorities on regular SARIB
meetings and local meetings.
One of the achievements of the SARIB project was also linkage to EU SedNet project.
The most important dissemination activity was the connection of the SARIB project
with the SAVA COMMISSION that will as “END USER” of the SARIB results take
over the SARIB web page in November 2007.
It may be therefore concluded that the SARIB project reached “end users” that will benefit the
results of this three year intensive research conducted within the SARIB project.


CONTRIBUTION of partner No. 1 JSI to WP 6:

Within the WP 6 PARTNER No. 1 JSI performed according to deliverable

D6.2: Technical and scientific reports; Publications; Presentations.

several activities: presentations of the results of the SARIB project on International
conferences, publishing the results in Conferences' proceedings and publishing the results as
working reports. In addition, as a result of the SARIB project a doctoral dissertation was also
completed by a Ph.D. student. Moreower, on the invitation of the Sava Comission, the results



Page 40 of 53                                                                      29/11/2011
from teh SARIB project were also presented on a regular Sava Commission meeting in Banja
Luka in June 2007.

The mentioned contributions are listed below:

Published Scientific Conference Contribution (Conference Proceedings )
1. MILAĈIĈ, Radmila, ŠĈANĈAR, Janez. Estimation of the extent of pollution of selected
elements in sediments of the Sava river. V: 1st Riskbase Thematic workshop, May 17-
18,2007, Lisbon, Portugal. Monitoring and assessment of river pollutants: a step towards the
implementation of risk based management plans, Case studies of Portuguese river basins,
(Riskbase). 2007, str. 64-69. [COBISS.SI-ID 20768039] INVITED TALK. (Annex 1.4.1.,
Annex 1.4.2., Annex 1.4.3.).
2. OGRINC, Nives, KANDUĈ, Tjaša, VAUPOTIĈ, Janja. Isotopic characteristics of the Sava
river basin in Slovenia. V: POVINEC, P. (ur.), SANCHEZ-CABEZA, J. A. (ur.).
Radionuclides in the environment : International Conference on Isotopes in Environmental
Studies : Aquatic Forum 2004, 25-29 October, Monaco, (Radioactivity in the environment,
vol. 8). Amsterdam; London: Elsevier, 2006, str. 317-325. [COBISS.SI-ID 19825191]
(Annex 1.10.)
Published Scientific Conference Contribution Abstract
1. MILAĈIĈ, Radmila, ŠĈANĈAR, Janez, MURKO, Simona, ZULIANI, Tea,
MILIVOJEVIĈ, Tadeja, HORVAT, Milena, KOCMAN, David, HEATH, Ester. Metals and
organic pollutants in sediments of Sava river. V: Sediment key-issues between the river and
the sea : SedNet conference on 23-24 November 2006, Venice, Italy. [Apeldoorn]: SedNet,
2006, str. P-10. [COBISS.SI-ID 20340007] (Annex 1.3.1., Annex 1.3.2.)
2. SZRAMEK, Kathryn, WALTER, Lynn M., OGRINC, Nives, KANDUĈ, Tjaša. Dolomite
mineral weathering contributions to the bicarbonate flux from headwater streams in the
Danube river basin. V: GSA Annual Meeting & Exposition. GSA Annual Meeting &
Exposition : 22-25 October 2006, Phyladelphia, PA. [S. l.]: The Geological Society of
America, 2006. [COBISS.SI-ID 20372519] (Annex 1.11.)

3. KANDUĈ, Tjaša, SZRAMEK, Kathryn, OGRINC, Nives, WALTER, Lynn M. Izvor in
kroţenje ogljika v reĉnem sistemu Save (Slovenija) z uporabo stabilnih izotopov = Source
and cycling of carbon in the river Sava (Slovenia) using stable isotope approach : Tjaša
Kanduĉ ... [et al.]. V: REŢUN, Bojan (ur.). 2. slovenski geološki kongres, Idrija, 26.-28.
september 2006. Zbornik povzetkov. Idrija: Rudnik ţivega srebra v zapiranju, 2006, str. 83-84.
[COBISS.SI-ID 20195111] (Annex 1.12.)


4. MARKOVICS, Roland, OGRINC, Nives, KANDUĈ, Tjaša, WALTER, Lynn M. Chemical
dynamics of the Sava riverine system - a stable isotopic approach : [presented at European
Geosciences Union General Assembly, 15-20 April 2007, Vienna, Austria]. Geophys. res.
abstr., 2007, vol. 9, str. 01859-1-01859-2. [COBISS.SI-ID 20729127] (Annex 1.13.)

5. MILJEVIĆ, N., GOLOBOĈANIN, D., OGRINC, Nives, MIHAJLOVIĆ, A. Distribution of
stable isotopes in surface water along the Danube river in Serbia : [presented at ESIR IX,
European Society for isotope Research, 23-28 June, 2007, Clij-Napoca, Bolgaria]. Stud. Univ.
Babeş-Bolyai, Geol., 2007, vol. 52, no. 1, str. 53. [COBISS.SI-ID 20890919] (Annex 1.14.)
Treatise, Preliminary Study, Study
1. ŠĈANĈAR, Janez, MURKO, Simona, ZULIANI, Tea, HORVAT, Milena, KOCMAN,
David, HEATH, Ester, MILAĈIĈ, Radmila. Establishment of the methodology for the


Page 41 of 53                                                                     29/11/2011
estimation of extent of pollution with metals and organic pollutants of the Sava river
sediments, (IJS delovno poroĉilo, 9579). 2007. [COBISS.SI-ID 20675623] (Annex 1.15.)
2. ŠĈANĈAR, Janez, MURKO, Simona, ZULIANI, Tea, HORVAT, Milena, KOCMAN,
David, HEATH, Ester, MILAĈIĈ, Radmila. Report on the contamination of the Sava river
sediments with metals and organic pollution, (IJS delovno poroĉilo, 9580). 2007.
[COBISS.SI-ID 20675367] (Annex 1.16.)

3. OGRINC, Nives, KANDUĈ, Tjaša, MARKOVICS, Roland, VAUPOTIĈ, Janja, ŢIGON,
Stojan, TRKOV, Zdenka, DUJMOVIĆ, Petra. Chemical and stable isotope investigation of
the Sava and Soča rivers in Slovenia, (IJS delovno poroĉilo, 9554). 2007. [COBISS.SI-ID
20663847] (Annex 1.17.)

Doctoral Dissertation

KANDUĈ, Tjaša. Hidrogeokemične značilnosti in kroženje ogljika v porečju reke Save v
Sloveniji: doktorska disertacija. Hydrogeochemical characterization and carbon cycling in the River
Sava watershed in Slovenia: Doctoral Dissertation, Ljubljana: [T. Kanduĉ], 2006. 141 f., [16] f. pril.,
ilustr., graf. prikazi. [COBISS.SI-ID 226716416] (Annex 1.18.)

Invitation of the Sava Commission to present the SARIB project:

1. MILAĈIĈ Radmila, RASPOR Biserka, GALIĆ Kata, KOPUSOVIĆ TARIK,
GLOBEVNIK Lidija, VRANEŠ Sanja, LOIBNER Andreas: presentation of the SARIB
project to the Sava Comission at 4th Sava PEG RBM Meeting, Banja Luka, Bosnia and
Herzegovina, June 11-12, 2007. (Annex 1.2.1., Annex 1.2.2.).


CONTRIBUTION of partner No. 2 UL FGG to WP 6:

According to deliverables:
D6.1: Web server, Mailing list, Usenet News, presentations to government and
nongovernmental organizations, CD and/or DVD.
D6.2: Technical and scientific reports; Publications; Presentations.

The following activities of partner No. 2 have been performed:

1.      SARIB GIS system put on web server and linked to SARIB web page
2.      Information on sampling and water quality analysis in the SARIB project has been
given in the ICPD /GEF UNDP DRP regional workshop for the task 2 in 3 (international
issues and programme of measures) on 12.November 2006, Sarajevo, Bosnia and
Herzegovina.
3.      Presentation and demonstration of SARIB GIS platform were given at the
Sava river Commission management board and Mangement plan working grup on
Regional workshop for the task 2 in 3 (international issues and programme of measures) in
April 2007, Sarajevo, Bosnia and Herzegovina.


CONTRIBUTION of partner No. 3 RBI to WP 6:
According to deliverable:



Page 42 of 53                                                                             29/11/2011
    D.6.2. Techical and scientific papers; Publications; Presentations

    In the third year of the project Partner 3 (RBI) contributed with 1 professional paper and 6
    presentations at the scientific and professional meetings. Particular attention has been given to
    inform possible beneficiary International Sava River Basin Commission about the activities
    and the selected results of the SARIB project, which could be of interest for Sava River Basin
    management plan. Detailes are available in Annex 3.10.



    CONTRIBUTION of partner No. 4 PBF to WP 6:

    According to deliverables:
    D6.1: Web server, Mailing list, Usenet News, presentations to government and
    nongovernmental organizations, CD and/or DVD.
    D6.2: Technical and scientific reports; Publications; Presentations.

    The following activities of partner No. 4 have been performed:

       Web-based activities:
    Web-based activities have been extended by creating internet domain and discussion forum.
    Based on the performed activities an Excel database is created for a quick and easy insight on
    the realised tasks. The results of activities are also published in peer-reviewed papers and
    Book of abstract and presented to interested parties – Sava Commission.

    Within the WP 6 (Dissemination), regarding the target to provide the most advanced and
    flexible communication platform for the SARIB project consortium members and for the
    Web-based delivery of the project interim and the results to the recipient institutions and
    individuals from the targeted region, the next actions are realised.
    Extension of sarib.net (www.sarib.net) and sarib.org (www.sarib.org) Internet domain with:
   Creation of intranet.sarib.net (http://intranet.sarib.net)
   Creation of SARIB discussion forum (http://forum.sarib.net/)

      Data collection-Excel database:
    Dissemination activities are presented in the Excel database under following titles: No.; Title;
    Presenters; Event / Publication; Date; Location; Partner No.; Partner name; Year; Country
    where activity has been held; Topics covered; Type of the dissemination.


      Published papers:
1. M. Sak-Bosnar, R. Matešić-Puaĉ, Z. Grabarić, New Potentiometric Sensor for Determination
   of Low Levels of Anionic Surfactants in Industrial Effluents, Tenside Surfactants Detergents,
   2 (2006) 82-87.
2. M. Sak-Bosnar, D. Madunić-Ĉaĉić, R. Matešić-Puaĉ, Z. Grabarić, "Nonionic surfactant-
   selective electrode and its application for determination in real solutions" Anal. Chim. Acta,
   581 (2007) 355-363.
3. M. Sak-Bosnar, D. Madunić-Ĉaĉić, R. Matešić-Puaĉ, Z. Grabarić, “Sensitive Potentiometric
   Method for Determination of Micromolar Level of Polyethoxylated Nonionic Surfactants in
   Effluents”, Tenside Surfactants Detergents, 44 (2007) 11-18.



    Page 43 of 53                                                                        29/11/2011
      Presentations at the scientific congresses:
1.        D.Madunić-Ĉaĉić, M. Sak-Bosnar, Z.Grabarić, Pseudocationic complexes of
   polyethoxylated nonionic surfactants and barium ion, XX. Croatian Meeting of Chemists and
   Chemical Engineers, 26 February- 1 March, 2007, Zagreb, Croatia. Book of abstracts, p.121.
2.        M. Sak-Bosnar, D. Madunić-Ĉaĉić, R. Matešić-Puaĉ, Z. Grabarić, A new sensitive
   potentiometric sensor for cationic surfactants, XX. Croatian Meeting of Chemists and
   Chemical Engineers, 26 February- 1 March, 2007, Zagreb, Croatia. Book of abstracts, p.190.

     Thesis:
1.      Dubravka Madunić-Ĉaĉić, Development and Application of Surfactant-Selective
     Electrodes for Waste Waters Monitoring, MSc thesis, University of Zagreb, Faculty of Food
     Technology and Biotechnology, November 15, 2006.

      Other public activities:
1.         R. Milaĉić, Dejan Komatina, B. Raspor, D.Kovaĉek, K.Galić, SARIB project
   introduction to SRB commission secretary, Meeting with Mr. Dejan Komatina -Secretary,
   International Sava River Basin Commission, 18 January 2007.
2.         R. Milaĉiĉ, B. Raspor, K. Galić, T. Kupusović, L. Globevnik, S. Vraneš, A. Loibner,
   Presentation of the SARIB project to the SAVA COMISSION, 4th Sava PEG RBM Meeting,
   June 11-12, 2007, Banja Luka, B&H. (Partners: 1-JSI, 3-RBI, 4-PBF, 5-HEIS, 2-ULFGG, 6-
   PUPIN, 10-NIVA)

     The planned activities were done in the third year of the SARIB project.


     CONTRIBUTION of partner No. 5 HEIS to WP 6:

     According to deliverables:
     D6.1: Web server, Mailing list, Usenet News, presentations to government and
     nongovernmental organizations, CD and/or DVD.
     D6.2: Technical and scientific reports; Publications; Presentations.

     The following activities of partner No. 5 have been performed:

     During the third project year, as a contribution to the D.6.1 - Web server, Mailing list, Usenet News,
     presentations to government and nongovernmental organizations, CD and/or DVD, and D.6.2 -
     Technical and scientific reports; Publications; Presentations, HEIS team members were presenting
     SARIB Project achievements covering activities within all six project work packages.

     During the Round table held for international and regional water and environment authorities titled as
     “Petersberg Phase II/Athens Declaration Process, Principles for Multipurpose Management in the Sava
     River Basin” and organized by the Sava Commission (15-18th November 2006 in Zagreb, Croatia),
     HEIS team had a presentation titled as: "FP6 - SARIB - Sava River Basin, Achievements after two
     years" (see Annex 5.11.).

     Apart from that, at the Conference titled as “FP6 projects in Bosnia and Herzegovina”, which was
     organized by the Rectorat of Sarajevo University Bosnia and Herzegovina (February 2007), aiming at
     promoting EC Framework Programs in B&H to the Faculties as well as governmental authorities,
     HEIS team members had a two presentations. The titles of the presentations were “Sava River Basin:
     Sustainable Use, Management and Protection of Resources” and “Research in EU – FP6 & FP7 HEIS
     Experiences” (see Annexes 5.12. and 5.13.).



     Page 44 of 53                                                                            29/11/2011
    CONTRIBUTION of partner No. 6 PUPIN to WP 6:

    According to deliverables:
    D6.1: Web server, Mailing list, Usenet News, presentations to government and
    nongovernmental organizations, CD and/or DVD.
    D6.2: Technical and scientific reports; Publications; Presentations.

    PUPIN activities related to WP6 has been:

           presented its main results on WP4 related to the Web repository of remediation
    technologies and DSS for remediation technology assessment and selection at the 4th Meeting
    of the Permanent Expert Group for Sava River Basin Management held on 12th of June, 2007
    in Banja Luka.
           published a paper “GIS-based Decision Support Tool for Remediation Technology
    Selection” in the Proceedings of the 5th IASME / WSEAS International Conference on
    HEAT TRANSFER, THERMAL ENGINEERING and ENVIRONMENT (HTE'07), August,
    Vouliagmeni, Greece, describing the integration of DSS for remediation technology
    assessment and selection into ARC GIS environment.


    CONTRIBUTION of partner No. 7 FABL to WP 6:

    According to deliverables:
    D6.1: Web server, Mailing list, Usenet News, presentations to government and
    nongovernmental organizations, CD and/or DVD.
    D6.2: Technical and scientific reports; Publications; Presentations.

    The following activities of partner No. 7 have been performed:
    According to deliverables D 6.1. and D 6.2 activities during the 3rd project year were focused
    on presentations to government and nongovernmental organisations as well as other relevant
    stakeholders in Bosnia and Herzegovina.

    MAIN RESULTS:
    SARIB Project presentation in Srbac municipality – 26th April 2007. Main participants -
    stakeholders: representatives of local communities, non-governmental organizations, farmers,
    representatives of chemical industry (pesticides), and other stakeholders in Sava river basin
    (SRB) from Bosnia and Herzegovina.

    The planned activities were done in the third year of the SARIB project.


    CONTRIBUTION of partner No. 8 ICS UNIDO to WP 6:

    According to deliverables:
    D6.1: Web server, Mailing list, Usenet News, presentations to government and
    nongovernmental organizations, CD and/or DVD.
    D6.2: Technical and scientific reports; Publications; Presentations.

    The following activities of partner No. 8 have been performed:


    Page 45 of 53                                                                     29/11/2011
    ICS-UNIDO has promoted the dissemination of the overall project activities and results
    through international workshops/meetings and through its own already established networks
    and focal points.

    The project was presented in the following ICS-UNIDO international events:
-             Workshop on “POPs destruction technologies and decision-support tools”, 28-29
    August 2006, Mahmudia, Romania;
-             Workshop on “Protected area management in Mediterranean coastal areas: the
    importance of wildlife refugia and corridors within an urban and industrial landscape”, 11-13
    September 2006, Tunis, Tunisia;
-             Training-of-trainers course on “The application of sustainability indicators in coastal
    areas: a landscape ecology approach to environmental management”, 18-22 September 2006,
    Grosseto, Italy;
-             Training Course on “Integrated Coastal Area Management”, 23-27 October 2006,
    Antofagasta, Chile;
-             Workshop on “Water and Soil Remediation Technologies”, 11-12 December 2006,
    Guanajuato, Mexico;
-             Training workshop on “GIS based decision-support tools for optimal site planning of
    industrial development in developing countries”, 11-15 December 2006, Trieste, Italy;
-             Expert Group meeting on “Emerging technologies for water treatment and soil
    remediation and their assessment”, 19-20 March 2007, Trieste, Italy;
-             Workshop on “Technologies for Environmental Clean-Up and DST for their
    Assessment and Selection”, 21-23 May 2007, Marrakech, Morocco;
-             Workshop on “The utilization of GIS for streamlining organizational and support
    services within an industrial cluster environment”, 6-8 June 2007, Port Elisabeth, South
    Africa.

    Moreover a link to SARIB web site has been activated in the ICS-UNIDO official web site:
    (http://www.ics.trieste.it/services_projects.aspx)


    CONTRIBUTION of partner No. 9 BOKU Wien to WP 6:

    According to deliverables:
    D6.2: Technical and scientific reports; Publications; Presentations.

    The following activities of partner No. 9 have been performed:

    Rresults on the bioavailability study and ecotoxicological evaluation of samples from the river
    Sava have been used for preparation of a master thesis work and scientific paper:

           Master thesis work:
    Wruss, K. Bioavailability assessment of hydrophobic organic pollutants in sediments
    investigated by biotests. Master Thesis, Vienna University of Technology, Faculty of
    Technical Chemistry, 13th March 2007

          Paper in preparation:



    Page 46 of 53                                                                        29/11/2011
Todorovic, D., Erlacher, E., Braun, R. and Loibner, A.P. Ecotoxicological evaluation of
pore water and sediments from Sava River Basin using selected bioassays

DEVIATION FROM TECHNICAL ANNEX:
All planed activities of partner No. 9 BOKU Wien in WP6 were performed according to plan.


CONTRIBUTION of partner No. 10 NIVA to WP 6:

According to deliverables:
D6.2: Technical and scientific reports; Publications; Presentations.

NIVA has, apart from publishing, presented data from the SARIB project as posters at an
international scientific meeting:

Publications
Grung, M., Lichtenthaler, R., Ahel, M., Tollefsen, K.E., Langford, K. and Thomas, K.V.
(2007) Effects-directed analysis of organic toxicants in wastewater effluent from Zagreb,
Croatia. Chemosphere. 40: 108-120. (See annex 10)
Källqvist, T., Milačič, R., Smital, T., Thomas, K. V., Vranes, S. and Tollefsen, K. E.
(Accepted with monor revisions). Chronic toxicity of the Sava river (SE Europe) sediments
and river water to the algae Pseudokirchneriella subcapitata. Water Research. (See annex 10)

Presentations
Nilsen, A.J., Tollefsen, K.E. (2007) Determination of DNA damage in rainbow trout primary
hepatocytes after exposure to organic chemicals and complex environmental samples by the
Fast Micromethod. SETAC Europe meeting, Porto. (Poster highlight).
Källqvist, T., Gregersen, I.K., Harman,C., Thomas, K.V., Tollefsen, K.E.(2007)
Assessment of sediment and river water toxicity by a suite of small-scale bioassays: a case-
study of the Sava River (SE Europe). SETAC Europe meeting, Porto. (Poster).



The dissemination activities of all partners in the third year presented in the following table:




Page 47 of 53                                                                         29/11/2011
    Activities of SARIB partners for the period from 1.8.2006. to 31.7.2007.


                                                                                     Place,     Partner
No.   Title                 Authors          Event                   Date                                 Partner
                                                                                     type         No.

      Levels of                              26th International
      polychlorinated                        Symposium on
      biphenyls and                          Halogenated             August 21-25,   Oslo,
1                                                                                                 3          RBI
      chlorinated                            Persistant Organic      2006            Norway
      insecticides in the                    Pollutants - DIOXIN
                            Violeta Ĉalić,
      liver and muscle                       2006.
                            Nena Picer,
      tissues of the
                            Mladen Picer
      European chub                          Proceedings             Proceedings,
      (Leuciscus                             Organohalogen           Vo. 68          Printed
2                                                                                                 3          RBI
      cephalus) from the                     compounds vol           pp.724-727,     material
      Sava River                             68:724-727              2006
      (Croatia)
      Metallothionein
                            Marijana
      and metal
                            Podrug,
      (Cu,Zn,Cd)
                            Zrinka
      concentrations in
2                           Dragun,                                                               3          RBI
      liver cytosol of
                            Marijana Erk,
      chub (Leuciscus
                            Biserka
      cephalus L.) from
                            Raspor
      Sava river
      ECOTOXICOLOG
                                             9th Croatian            September 23-   Rovinj,
      ICAL
                                             Biological Congress     29, 2006        Croatia
      INDICATORS OF
      POLLUTION IN
      THE EUROPEAN
4     CHUB (Leuciscus       Sanja Krĉa                                                            3          RBI
      cephalus L.) -
      RESEARCH
      WITHIN THE EU
      FP6 "SARIB"
      PROJECT
      Determination of
                                             Doctoral Thesis,
      bioavailable metal
                                             University of Zagreb,
      concentrations in                                              November        Zagreb,
5                           Zrinka Dragun    Faculty of Science,                                  3          RBI
      chub (Leuciscus                                                15th, 2006      Croatia
                                             Department of
      cephalus L.) gills
                                             Biology
      and in water
      Development and
      Application of                         MSc thesis,
      Surfactant-           Dubravka         University of Zagreb,
                                                                     November 15,    Zagreb,
6     Selective             Madunić-         Faculty of Food                                      4          PBF
                                                                        2006.        Croatia
      Electrodes for        Ĉaĉić            Technology &
      Waste Waters                           Biotechnology
      Monitoring
                                             Workshop-
                                             Biomonitoring of
      SARIB project:
                                             surface waters in the
      Key activities
                            Biserka          Sava river sub-basins   December 6th,   Zagreb,
7     relevant for WFD                                                                            1,3     JSI, RBI
                            Raspor           2006-progress ,         2006            Croatia
      implementation in
                                             results and
      Sava River Basin
                                             determination of
                                             ecological status




    Page 48 of 53                                                                               29/11/2011
                              Tollefsen, K.
                              E., Bratsberg,
       Use of fish in vitro   E., Bøyum,
       hepatocyte assays      O., Finne, E.
       to detect multi-       F., Gregersen,   Mar. Environ. Res.                      Printed
8                                                                          2006                      10      NIVA
       endpoint toxicity in   I. K.,           62: S356-S359.                          material
       Slovenian River        Hegseth, M.
       sediments              N., Sandberg,
                              C., Hylland,
                              K.
                                               Hrvatska
       SARIB -što je to?
                              Biserka          vodoprivreda,            November       Zagreb,
10     (SARIB-What is                                                                                3          RBI
                              Raspor           Zagreb, studeni            2006         Croatia
       that?)
                                               2006., broj 167
                                               Petersberg Phase
       FP6 - SARIB -                           II/Athens Declaration
       Sava River Basin:      Tarik            Process, Principles     November 15-    Zagreb,
11                                                                                                   5       HEIS
       Achievements after     Kupusović        for Multipurpose          17 2006       Croatia
       two years                               Management in the
                                               Sava River Basin
                                               Petersberg Phase
                                               II/Athens Declaration
       Sava Navigation -
                              Tarik            Process, Principles     November 15-    Zagreb,
12     Introductory                                                                                  5       HEIS
                              Kupusović        for Multipurpose          17 2006       Croatia
       Remarks
                                               Management in the
                                               Sava River Basin
       Hydrogeochemical
       characteristics and
       cycling of carbon
       in the River Sava
                                               Doctoral Thesis,
       watershed in                                                                    Ljubljana
                              KANDUĈ,          Ljubljana,
13     Slovenia =                                                          2006        ,             1          JSI
                              Tjaša            [COBISS.SI-ID
       Hidrogeokemiĉne                                                                 Slovenia
                                               226716416]
       znaĉilnosti in
       kroţenje ogljika v
       poreĉju reke Save v
       Sloveniji
       Nonionic               M. Sak-
       surfectant-selective   Bosnar, D.
       electrode and its      Madunić-         Anal. Chim. Acta,                       Printed
14                                                                         2007                      4          PBF
       application for        Ĉaĉić, R.        581 (2007) 355-363.                     material
       detemination in real   Matešić-Puaĉ,
       solutions              Z. Grabarić
                              R. Milaĉić,      Meeting with Mr.
       SARIB project          Dejan            Dejan Komatina -
       introduction to SRB    Komatina, B.     Secretary,               18 January     Zagreb,              JSI, RBI,
15                                                                                                  1,3,4
       commission             Raspor,          International Sava          2007        Croatia                PBF
       secretary              D.Kovaĉek,       River Basin
                              K.Galić          Commission
       Comparison of the
       histological
       changes in organs
       of chub (Leuciscus
                                               Doctoral Thesis,
       cephalus) with the     Boţidar                                  February 1st,   Zagreb,
16                                             University of Zagreb,                                 3          RBI
       number of              Kurtović                                    2007         Croatia
                                               Veterinary Faculty
       heterotrophic and
       coliform bacteria in
       the water of Sava
       River




     Page 49 of 53                                                                                 29/11/2011
       Practical
       experiences in the                      Meeting at the                           Sarajevo,
                              Tarik
       participation in                        Rectorate of Sarajevo    February 27     Bosnia &
17                            Kupusović                                                               5      HEIS
       FP6/EU - SARIB                          University for              2007         Herzego
                              Selma Ĉengić
       Project - Goals and                     FP6/FP7 Focal Points                     vina
       results
       Sensitive
       Potentiometric
                              M. Sak-
       Method for
                              Bosnar, D.
       Determination of                        Tenside Surfactants
                              Madunić-                                                  Printed
18     Micromolar Level                        Detergents, 44 (2007)       2007.                      4          PBF
                              Ĉaĉić, R.                                                 material
       of Polyethoxylated                      11-18
                              Matešić-Puaĉ,
       Nonionic
                              Z. Grabarić
       Surfactants in
       Effluents

                                                                       26 February- 1   Zagreb,
19     Pseudocationic                                                                                 4          PBF
                              D.Madunić-                                March, 2007     Croatia
       complexes of
                              Ĉaĉić, M. Sak-
       polyethoxylated
                              Bosnar,
       nonionic surfactants                                               Book of
                              Z.Grabarić                                                Printed
20     and barium ion                          XX. Croatian              abstracts,                   4          PBF
                                                                                        material
                                               Meeting of Chemists      p.121, 2007
                                               and Chemical
                              M. Sak-          Engineers               26 February- 1   Zagreb,
21                                                                                                    4          PBF
       A new sensitive        Bosnar, D.                                March, 2007     Croatia
       potentiometric         Madunić-
       sensor for cationic    Ĉaĉić, R.                                   Book of
       surfactants            Matešić-Puaĉ,                                             Printed
22                                                                       abstracts,                   4          PBF
                              Z. Grabarić                                               material
                                                                        p.190, 2007
                                               Stakeholders:
                                               farmers,
                                               representatives of
                                               chemical industry
       SARIB presentation                      (pesticides), non-
                              Mihajlo                                                   Srbac,
23     in Srbac                                governmental            April 26, 2007                 7      FABL
                              Marković                                                  B&H
       municipality                            organizations, local
                                               communities and
                                               other stakeholders in
                                               Sava river basin
                                               (SRB).
       Bioavailability
       assessment of                           Master Thesis,
       hydrophobic                             Vienna University of
                                                                       March 13th,      Vienna,
24     organic pollutants     Klaus Wruss      Technology, Faculty                                    9      BOKU
                                                                       2007             Austria
       in sediments                            of Technical
       investigated by                         Chemistry
       biotests
                                               1st Riskbase
                                               Thematic workshop.      May 17-18,       Lisbon,
25                                             Monitoring and                                         1          JSI
       Estimation of the                                               2007             Portugal
                                               assessment of river
       extent of pollution    MILAĈIĈ,
                                               pollutants: a
       of selected elements   Radmila,
                                               steptowards the
       in sediments of the    ŠĈANĈAR,
                                               implementation of       Proceedings,
       Sava river - Invited   Janez                                                     Printed
26                                             risk based              pp. 64-69,                     1          JSI
       lecture                                                                          material
                                               management plans,       2007
                                               Case studies of
                                               Portuguese river



     Page 50 of 53                                                                                  29/11/2011
                                                   basins, (Riskbase),
                                                   p.p. 64-69.



                                 Radmila
                                 Milaĉiĉ,
                                 Biserka
                                                                                                                   JSI, RBI,
                                 Raspor, Kata
       Presentation of the                                                                                           PBF,
                                 Galiĉ, Tarik                                             Banja
       SARIB project to                            4th Sava PEG RBM       June 11-12,                1, 3, 4, 5,     HEIS,
27                               Kopusović,                                               Luka,
       the SAVA                                    Meeting,               2007                        2, 6, 10     ULFGG,
                                 Lidija                                                   B&H
       COMISSION                                                                                                    PUPIN,
                                 Globevnik,
                                                                                                                     NIVA
                                 Sanja Vraneš,
                                 Andreas
                                 Loibner
                                                   27th International
       Organochlorines                             Symposium on
       from the Sava River                         Halogenated
                                 Ĉalić V, Picer                           September 2-7   Tokyo,
28     (Croatia), levels in                        Persistant Organic                                    3           RBI
                                 M, Picer N                                   2007        Japan
       fish and SPMD                               Pollutants - DIOXIN
       uptake                                      2007. Organohalogen
                                                   compounds vol 69
       Determination        of
       Acanthocephala
       parasites            in
                                 Vardić I.,
       European          chub
                                 Kurtović B.,
       (Leuciscus
                                 Valić D.,
       cephalus)         from                      XII European                           Cavtat,
                                 Kapetanović                              September 9-
29     Sava river based on                         Congres of                             Dubrovni       3           RBI
                                 D.,                                      13 2007
       mitochondrial                               Ichthyology                            k
                                 Teskeredţić
       DNA, 18S and ITS
                                 Z.,
       rDNA         sequence
                                 Teskeredţić E.
       analysis         –Best
       poster presentation
       award
       The seasonal
       influence on the
       biotic factors and
       the cytosolic metal       Z. Dragun, B.
                                                   Chemosphere, 69 (6):                   Printed
30     concentrations in         Raspor, M.                                   2007                       3           RBI
                                                   911-919                                material
       the gills of the          Podrug
       European chub
       (Leuciscus
       Cephalus L.)
       Hepatic biomarker
       responses to
       organic                   S. Krĉa, R.
       contaminants in           Ţaja, V. Ĉalić,
                                                   Environmental
       feral chub                S. Terzić,
                                                   Toxicology and                         Printed
31     (Leuciscus                M.S.                                         2007                       3           RBI
                                                   Chemistry,                             material
       cephalus)-                Grubešić, M.
                                                   doi:10.1897_07-227
       Laboratory                Ahel, T.
       characterisation and      Smital
       field study in the
       Sava River, Croatia




     Page 51 of 53                                                                                   29/11/2011
                                               Proceedings of the
                                               5th IASME / WSEAS
       GIS-based Decision     Jovan            International
       Support Tool for       Dudukovic,       Conference on HEAT                        Vouliag
32     Remediation            Mladen           TRANSFER,                August 2007      meni,        6      Pupin
       Technology             Stanojevic,      THERMAL                                   Greece
       Selection              Sanja Vranes     ENGINEERING and
                                               ENVIRONMENT
                                               (HTE'07)
                              Grung, M.,
       Effects-directed
                              Lichtenthaler,
       analysis of organic
                              R., Ahel, M.,
       toxicants in                            Chemosphere,                              Printed             NIVA,
33                            Tollefsen,                                    2007                     10, 3
       wastewater effluent                     67(1):108-120                             material             RBI
                              K.E.,
       from Zagreb,
                              Langford, K.,
       Croatia
                              Thomas, K.V.
                              Radmila
                              Milacic, Janez
                              Šcancar,
       Pollution of           Milena
                                                                        September        Printed
34     Sediments of the       Horvat, Ester    SedNet Newsletter,                                     1          JSI
                                                                        2007             material
       SAVA River             Heath, Simona
                              Murko, David
                              Kocman, Tea
                              Zuliani
       Hydrogeochemical
       characteristics of
       the river Sava
                              KANDUĈ,          Geologija, 2007, vol.
       watershed in
                              Tjaša,           50, no. 1, str. 157-                      Printed
35     Slovenia =                                                           2007                      1          JSI
                              OGRINC,          177. [COBISS.SI-ID                        material
       Hidrogeokemiĉne
                              Nives.           20935207]
       znaĉilnosti poreĉja
       reke Save v
       Sloveniji.
       Origin and cycling
       of riverine            KANDUĈ,          Biogeochemistry
       inorganic carbon in    Tjaša,           (Dordrecht), 2007, 18
       the Sava River         SZRAMEK,         str.
                                                                                         Printed
36     watershed              Kathryn,         http://dx.doi.org/10.1       2007                      1          JSI
                                                                                         material
       (Slovenia) inferred    OGRINC,          007/s10533-007-
       from major solutes     Nives,           9149-4. [COBISS.SI-
       and stable carbon      WALTER,          ID 21023271]
       isotopes.              Lynn M.

                                                                        15-20 April      Vienna,
37                            MARKOVICS                                                               1          JSI
                                                                        2007,            Austria
                              , Roland,
       Chemical dynamics      OGRINC,
                                               European                 Book of
       of the Sava riverine   Nives,
                                               Geosciences Union        abstracts, Vol
       system - a stable      KANDUĈ,
                                               General Assembly,        9. p.01859-1-    Printed
38     isotopic approach      Tjaša,                                                                  1          JSI
                              WALTER,                                   01859-2.         material
                              Lynn M.                                   [COBISS.SI-
                                                                        ID 20729127]
                              Ogrinc, Nives,
       Sources and
                              Markovics,
       distribution of
                              Roland,          Applied                  Submitted        Printed
39     carbon and nitrogen                                                                            1          JSI
                              Kanduĉ,          Geochemistry             2007             material
       in the River Sava
                              Tjaša,
       watershed.
                              Walters, M.



     Page 52 of 53                                                                                  29/11/2011
                               Lynn,




                               Markovics,
                               Roland,
                               Ogrinc, Nives,
       Chemical dynamics       Kanduĉ,
                                                Aquatic          Submitted       Printed
40     of the Sava riverine    Tjaša,                                                         1          JSI
                                                Geochemistry     2007            material
       system                  Walters, M.
                               Lynn,
                               Szramek,
                               Kathryn,
       Ecotoxicological
       evaluation of pore
                               Todorovic, D.,
       water and
                               Erlacher, E.,                       (paper in
39     sediments from                                                                         9      BOKU
                               Braun, R. and                      preparation)
       Sava River Basin
                               A.P.Loibner
       using selected
       bioassays
       The resultes of the
       analysis of heavy
       metals in sediment                                          (paper in
40                                                                                            5      HEIS
       from main B&H's         M. Dţonlić,                        preparation)
       Sava river              T. Kupusović,
       tributaries             S. Ĉengić
       A           complex     Radmila
       investigation of the    Milacic, Janez
       extent of pollution     Šcancar,
       of           selected   Milena
                                                Environment        Submitted
41     elements         and    Horvat, Ester                                                  1          JSI
                                                Interanational       2007
       persistent organic      Heath, Simona
       pollutants         in   Murko, David
       sediments of the        Kocman, Tea
       Sava River              Zuliani




     Page 53 of 53                                                                          29/11/2011

				
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