BIRLA INSTITUTE OF TECHNOLOGY & SCIENCE, PILANI
INSTRUCTION DIVISION
Course Handout (Part II)
In addition to part I (General Handout for all courses appended to the time table) this portion gives
further specific details regarding the course.
Course No. : BIO C461
Course Title : Recombinant DNA Technology
1. Course Description : The course deals with theoretical aspects of recombinant DNA
manipulation. Emphasis will be plant on procedures to create chimeric molecular using examples
from actual experimented work. Vector designing, polymers chain reaction, invitio mulagenesed
cloning in prokaryote and unpatriotic vectors will be covered.
2. Scope and Objective : This course aims to introduce the principles of recombinant DNA
Technology. Representative types of cloning vectors will be surveyed along with techniques of
transferring the rDNA into host cells. PCR, in nitromolagansis and other advanced topics will be
covered.
3. TextBook: T1 Old R W & S B Primrose, Principles of Gene Manipulations. OUP, 5th
ed. 1994.
4. Reference Books :
R1. . Recombinant DNA Principles & Methodologies, James J. Green & Vengalla B. Rao.
Marcel Dekker Inc - 1998.
R2. R.L. Rodriguez and R.C. Tait, Recombinant DNA Techniques -An Introduction, The
Benjamin Cummings Publishing company. Inc., CA, USA, 1983.
5. Course Plan:
Lec. No. Learning Objectives Topics to be covered Ref* Chap./Sec.# Book
1&2 Introductory concepts An overview of rDNA Text Book Ch.
technology
3,4,5 Techniques Western, Southern, Text Book Ch.2
Northern
6,11 Making recombinant molecules Linkers, Adaptors etc Text Book Ch.3
Homopolymer tailing
12-17 Plasmids Plasmids as cloning Text Book Ch.6
vehicles (vector)
18-21 Vectors Bacteriophage & Text Book Ch.5
Cosmid Vectors
22-24 Sequences Analysing DNA Text Book Ch.9
sequences
25-29 PCR Understanding PCR, Text Book Ch.10
Inverse PCR
30-34 Site directed mutagenesis The single primer Text Book Ch.11
method, the PCR
method
35-38 Cloning Strategies Different cloning Text Book Ch.6
strategies
39-40 Recombinant Selection & Screening Screening of Text Book Ch.7
Recombinant
41-42 Expression in E.coli Text Book Ch.7
12. Applications of rDNA Technology Assignment & Seminar
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Total Lectures 42
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6. Evaluation Scheme :
EC No Evaluation Component Duration Weightage Date, Time Venue Remarks
1. Test I 50 mts 20 CB
2. Test II 50 mts 20 CB
3. Assignment & Seminar 30
4. Compre. Exam. 3 hrs. 30 OB
7. Chamber Consultation Hour: To be announced in the class
8. Notice : Notices concerning the course will be put on Bio. Sciences Group NB only.
9. Make-up Policy : No make-ups without prior intimation or only in case of hospitalization
Instructor-in-charge
BIO C461