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Alcian Blue Stain

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					Alcian Blue Stain




   理                1
                     PURPOSE

Alcian blue stains acid mucosubstances and acetic mucins.




                                                      2
                       PRINCIPLE
Alcian blue is a copper phthalcyanin dye and contains
positively charged groups capable of salt linkage with certain
polyanions.
These polyanions consist of the sulphate and carbocyl radicals
of the acid mucins .
Varying the PH of the solution of Alcian blue more information
can be gained concerning the types of acid mucin present.



                                                            3
                   SPECIMEN

Tissue   skin tissue
Fixative formalin fixed, paraffin embedded tissue sections.




                                                        4
       Solutions
3% Acetic acid solution
Alcian blue solution(PH 2.5)
Nuclear fast red solution




                               5
                 PROCEDURE
1. Deparaffinize slides and hydrate to tap water.
2. Treat in 3% acetic acid solution for 3 min.
3. Wash in tap water.
4. Stain in Alcian blue solution (PH 2.5) for 30 min.
5. Wash in running tap water for 2 min.
6. Counterstain in nuclear fast red solution for 5 min.
7. Wash in running tap water for 1 min.
8. Dehydrate, clear and mount sections.

                                                          6
             RESULTS

Strongly acidic mucosubstances   blue.
Nuclei       pink to red.
Cytoplasm       pale pink.




                                         7
H&E stain          Alcian blue stain




            10 X                          X
                                       10 8
H&E stain          Alcian blue stain




            40 X               40 X
                                       9
10

				
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posted:11/28/2011
language:English
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