Supplementary Information Table 2. Proteins coimmunoprecipitated with wild type EGFR before (Cont) and after (EGFR) EG
mass spectrometer, and selected with three criteria: 1) more than 95% peptide identification probability, 2) more than 95%
peptides for a protein were normalized to the bait, EGFR peptides. Proteins were sorted according to the assigned peptide n
experimental repeats. The percentage sequence coverage, number of unique peptides, number of unique spectra and prote
coverage.
Accesion nubmer Protein Annotation
IPI00018273 EGFR unphosphorylated (normalized)
(Origianl peptide number)
EGFR phosphorylated peptides
IPI00470657 Anti-colorectal carcinoma heavy chain
IPI00304925 HSPA1B;HSPA1A Heat shock 70 kDa protein 1
IPI00027269 CBL E3 ubiquitin-protein ligase CBL
IPI00011654 TUBB Tubulin beta chain
IPI00064328 PRMT5 protein arginine methyltransferase 5 isoform b
IPI00021327 GRB2 Isoform 1 of Growth factor receptor-bound protein 2
IPI00012079 EIF4B Eukaryotic translation initiation factor 4B
IPI00020131 SOS1 Son of sevenless homolog 1
IPI00382470 HSP90AA1 heat shock protein 90kDa alpha (cytosolic)
IPI00021439 ACTB Actin, cytoplasmic 1
IPI00162330 MRPL37 39S ribosomal protein L37, mitochondrial precursor
IPI00104050 THRAP3 Thyroid hormone receptor-associated protein 3
IPI00375731 RBM10 Putative uncharacterized protein DKFZp686E2459
IPI00027251 STK38 Serine/threonine-protein kinase 38
IPI00012202 WDR77 Methylosome protein 50
IPI00783656 MRPL38 39S ribosomal protein L38, mitochondrial precursor
IPI00552587 GADD45GIP1 Growth arrest and DNA-damage-inducible proteins-interacting protein 1
IPI00023086 MRPL15 39S ribosomal protein L15, mitochondrial precursor
IPI00743194 Kappa light chain variable region (Fragment)
IPI00021326 SHC1 SHC (Src homology 2 domain containing) transforming protein 1
IPI00414676 HSP90AB1 Heat shock protein HSP 90-beta
IPI00000874 PRDX1 Peroxiredoxin-1
IPI00007750 TUBA4A Tubulin alpha-4A chain
IPI00008868 MAP1B Microtubule-associated protein 1B
IPI00003865 HSPA8 Isoform 1 of Heat shock cognate 71 kDa protein
IPI00009680 MRPL44 39S ribosomal protein L44, mitochondrial precursor
IPI00216049 HNRPK Isoform 1 of Heterogeneous nuclear ribonucleoprotein K
IPI00444262 NCL cDNA FLJ45706 fis, clone FEBRA2028457, highly similar to Nucleolin
IPI00023334 MRPL4 Isoform 1 of Mitochondrial 39S ribosomal protein L4
IPI00549381 MRPL1 mitochondrial ribosomal protein L1 precursor
IPI00007001 MRPL11 39S ribosomal protein L11, mitochondrial precursor
IPI00084571 MRPL39 Isoform 2 of Mitochondrial 39S ribosomal protein L39
IPI00514506 MRPL24 39S ribosomal protein L24, mitochondrial precursor
IPI00027096 MRPL19 39S ribosomal protein L19, mitochondrial precursor
IPI00010278 MRPS30 Mitochondrial 28S ribosomal protein S30
IPI00011253 RPS3 40S ribosomal protein S3
IPI00026612 PPM1B Isoform Beta-1 of Protein phosphatase 1B
IPI00030820 MRPL47 39S ribosomal protein L47, mitochondrial precursor
IPI00031812 YBX1 Nuclease-sensitive element-binding protein 1
IPI00022403 MRPL13 39S ribosomal protein L13, mitochondrial
IPI00012353 MRPL3 Mitochondrial 39S ribosomal protein L3
IPI00029114 ICT1 Immature colon carcinoma transcript 1 protein precursor
IPI00185859 MRPL45 39S ribosomal protein L45, mitochondrial precursor
IPI00172591 MRPL17 39S ribosomal protein L17, mitochondrial precursor
IPI00307409 MRPL9 39S ribosomal protein L9, mitochondrial precursor
IPI00019459 MAP3K7IP1 Mitogen-activated protein kinase kinase kinase 7-interacting protein 1
IPI00414410 MRPL22 Isoform 1 of 39S ribosomal protein L22, mitochondrial precursor
IPI00396218 SCYL2 SCY1-like protein 2
IPI00000821 MRPL16 39S ribosomal protein L16, mitochondrial precursor
IPI00302592 FLNA filamin A, alpha isoform 1
IPI00479217 HNRNPU Isoform Short of Heterogeneous nuclear ribonucleoprotein U
IPI00063242 PGAM5 Isoform 2 of Phosphoglycerate mutase family member 5 precursor
IPI00025447 EEF1A1 Elongation factor 1-alpha
IPI00106955 LOC144097 Uncharacterized protein LOC144097
IPI00292000 PRPF31 Isoform 1 of U4/U6 small nuclear ribonucleoprotein Prp31
IPI00171336 RIOK1 Serine/threonine-protein kinase RIO1
IPI00005537 MRPL12 39S ribosomal protein L12, mitochondrial precursor
IPI00006079 BCLAF1 Isoform 1 of Bcl-2-associated transcription factor 1
IPI00299155 PSMA4 Proteasome subunit alpha type-4
IPI00007765 HSPA9 Stress-70 protein, mitochondrial precursor
IPI00013296 RPS18;LOC100130553 40S ribosomal protein S18
IPI00172594 MRPL28 39S ribosomal protein L28, mitochondrial precursor
IPI00018691 MRPS18A 28S ribosomal protein S18a, mitochondrial precursor
IPI00004977 VAV2 vav 2 guanine nucleotide exchange factor
IPI00375677 MRPL21 39S ribosomal protein L21, mitochondrial precursor
IPI00399254 OTUD4 Isoform 1 of OTU domain-containing protein 4
IPI00218566 MAP3K7 Isoform 1A of Mitogen-activated protein kinase kinase kinase 7
IPI00013706 LOC642393;MRPL20 39S ribosomal protein L20, mitochondrial precursor
IPI00099871 MRPL40 39S ribosomal protein L40, mitochondrial precursor
IPI00010740 SFPQ Isoform Long of Splicing factor, proline- and glutamine-rich
IPI00013881 HNRPH1 Heterogeneous nuclear ribonucleoprotein H
IPI00304596 NONO Non-POU domain-containing octamer-binding protein
IPI00003362 HSPA5 HSPA5 protein
IPI00329036 MRPL50 Mitochondrial 39S ribosomal protein L50
IPI00221106 SF3B2 splicing factor 3B subunit 2
IPI00440493 ATP5A1 ATP synthase subunit alpha, mitochondrial precursor
IPI00020984 CANX Calnexin precursor
IPI00004511 PFKFB3 Isoform 1 of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
IPI00411816 MRPL2 39S ribosomal protein L2, mitochondrial precursor
IPI00013122 CDC37 Hsp90 co-chaperone Cdc37
IPI00295066 MRPL48 Isoform 1 of 39S ribosomal protein L48, mitochondrial precursor
IPI00293476 MRPL23 Mitochondrial 39S ribosomal protein L23
IPI00220644 PKM2 Isoform M1 of Pyruvate kinase isozymes M1/M2
IPI00166768 TUBA1C TUBA1C protein
IPI00334579 MRPL43 mitochondrial ribosomal protein L43 isoform d
IPI00007188 SLC25A5 ADP/ATP translocase 2
IPI00217553 MRPL41 39S ribosomal protein L41, mitochondrial precursor
IPI00160421 MRPL18 39S ribosomal protein L18, mitochondrial precursor
IPI00023161 MRPL46 39S ribosomal protein L46, mitochondrial precursor
IPI00550655 SPIN1 Spindlin-1
IPI00221092 RPS16 40S ribosomal protein S16
IPI00237011 STK38L Serine/threonine-protein kinase 38-like
IPI00027107 TUFM Tu translation elongation factor, mitochondrial precursor
IPI00004795 CLNS1A Methylosome subunit pICln
IPI00009444 MRPL27 Mitochondrial 39S ribosomal protein L27
IPI00061531 MRPL53 39S ribosomal protein L53, mitochondrial precursor
IPI00007752 TUBB2C Tubulin beta-2C chain
IPI00396435 DHX15 Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
IPI00167638 GTPBP10 Isoform 1 of GTP-binding protein 10
IPI00170877 MRPL10 Uncharacterized protein MRPL10
IPI00217030 RPS4X 40S ribosomal protein S4, X isoform
IPI00642862 PPIL4 Peptidyl-prolyl cis-trans isomerase-like 4
IPI00021266 RPL23A;hCG_16001 60S ribosomal protein L23a
IPI00026089 SF3B1 Splicing factor 3B subunit 1
IPI00013195 MRPL49 Mitochondrial 39S ribosomal protein L49
IPI00221088 RPS9 40S ribosomal protein S9
IPI00296337 PRKDC Isoform 1 of DNA-dependent protein kinase catalytic subunit
IPI00219018 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
IPI00011077 MRPL32 39S ribosomal protein L32, mitochondrial precursor
IPI00419880 RPS3A 40S ribosomal protein S3a
IPI00465361 RPL13 60S ribosomal protein L13
IPI00216587 RPS8 40S ribosomal protein S8
IPI00290566 TCP1 T-complex protein 1 subunit alpha
IPI00554793 MYCBP C-Myc-binding protein
IPI00217465 HIST1H1C Histone H1.2
IPI00215780 RPS19 40S ribosomal protein S19
IPI00418290 MRPL14 39S ribosomal protein L14, mitochondrial precursor
IPI00010153 RPL23 60S ribosomal protein L23
IPI00026271 RPS14 40S ribosomal protein S14
IPI00419626 MRPL55 Isoform 2 of 39S ribosomal protein L55, mitochondrial precursor
IPI00290770 CCT3 chaperonin containing TCP1, subunit 3 isoform b
IPI00220301 PRDX6 Peroxiredoxin-6
IPI00012750 RPS25 40S ribosomal protein S25
IPI00465176 MRPL30 Isoform 2 of 39S ribosomal protein L30, mitochondrial precursor
IPI00398135 hCG_21078 hypothetical protein LOC389435
IPI00000643 BAG2 BAG family molecular chaperone regulator 2
IPI00013485 RPS2 40S ribosomal protein S2
IPI00292856 CBLB Isoform Long of E3 ubiquitin-protein ligase CBL-B
IPI00306332 RPL24 60S ribosomal protein L24
IPI00299573 RPL7A 60S ribosomal protein L7a
IPI00179330 UBB;RPS27A;UBC ubiquitin and ribosomal protein S27a precursor
IPI00328616 MRPL52 39S ribosomal protein L52, mitochondrial precursor
IPI00025091 RPS11 40S ribosomal protein S11
IPI00008557 IGF2BP1 Insulin-like growth factor 2 mRNA-binding protein 1
IPI00018971 TRIM21 52 kDa Ro protein
IPI00028390 MRPL34 39S ribosomal protein L34, mitochondrial precursor
IPI00300371 SF3B3 Isoform 1 of Splicing factor 3B subunit 3
IPI00295992 ATAD3A Isoform 2 of ATPase family AAA domain-containing protein 3A
IPI00026302 RPL31 60S ribosomal protein L31
IPI00221089 RPS13 40S ribosomal protein S13
IPI00302927 CCT4 T-complex protein 1 subunit delta
IPI00784154 HSPD1 60 kDa heat shock protein, mitochondrial precursor
IPI00303476 ATP5B ATP synthase subunit beta, mitochondrial precursor
IPI00221091 RPS15A 40S ribosomal protein S15a
IPI00025329 RPL19 60S ribosomal protein L19
IPI00332157 MRPL54 39S ribosomal protein 54, mitochondrial precursor
IPI00029631 ERH Enhancer of rudimentary homolog
IPI00010720 CCT5 T-complex protein 1 subunit epsilon
IPI00007144 RPL26L1 60S ribosomal protein L26-like 1
IPI00786982 PGAM5 similar to phosphoglycerate mutase family member 5
IPI00411680 PCMT1 Isoform 1 of Protein-L-isoaspartate(D-aspartate) O-methyltransferase
IPI00216298 TXN Thioredoxin
IPI00607627 MRPL51 39S ribosomal protein L51, mitochondrial precursor
IPI00006024 DOCK4 Isoform 2 of Dedicator of cytokinesis protein 4
IPI00018146 YWHAQ 14-3-3 protein theta
IPI00024933 RPL12 60S ribosomal protein L12
IPI00329719 MYO1D Isoform 1 of Myosin-Id
IPI00031801 CSDA Isoform 1 of DNA-binding protein A
IPI00012011 CFL1 Cofilin-1
IPI00784013 JAK1 janus kinase 1
IPI00028888 HNRPD Isoform 1 of Heterogeneous nuclear ribonucleoprotein D0
IPI00106509 HNRPAB Isoform 4 of Heterogeneous nuclear ribonucleoprotein A/B
IPI00513971 RPS27 40S ribosomal protein S27
IPI00302176 NOLA1 Isoform 1 of H/ACA ribonucleoprotein complex subunit 1
IPI00029750 RPS24 Isoform 1 of 40S ribosomal protein S24
IPI00011736 PIK3R2 Phosphatidylinositol 3-kinase regulatory subunit beta
IPI00829827 Uncharacterized protein ENSP00000374804
IPI00216237 RPL36 60S ribosomal protein L36
IPI00216153 RPS15 40S ribosomal protein S15
IPI00413324 TCEB3C;TCEB3CL;RPL17;LOC100133240;LOC100133931 60S ribosomal protein L17
IPI00302925 CCT8 59 kDa protein
IPI00329389 RPL6 60S ribosomal protein L6
IPI00143753 SR140 Isoform 1 of U2-associated protein SR140
IPI00020050 DDX28 Probable ATP-dependent RNA helicase DDX28
IPI00012066 PCBP2 poly(rC)-binding protein 2 isoform b
IPI00000875 EEF1G Elongation factor 1-gamma
IPI00154910 UBASH3B Ubiquitin associated and SH3 domain-containing protein B
IPI00013214 MCM3 DNA replication licensing factor MCM3
IPI00026272 HIST1H2AE;HIST1H2AB Histone H2A type 1-B
IPI00215965 HNRNPA1 Isoform A1-B of Heterogeneous nuclear ribonucleoprotein A1
IPI00412607 RPL35 60S ribosomal protein L35
IPI00011694 PRSS1 Trypsin-1 precursor
IPI00024382 CLASP2 CLIP-associating protein 2
IPI00006864 MRPL33 Mitochondrial 39S ribosomal protein L33
IPI00013452 EPRS Bifunctional aminoacyl-tRNA synthetase
IPI00215790 RPL38 60S ribosomal protein L38
IPI00012493 RPS20 40S ribosomal protein S20
MAP3K7IP2 Isoform 2 of Mitogen-activated protein kinase kinase kinase 7-interacting
IPI00157561 protein 2
IPI00215637 DDX3X ATP-dependent RNA helicase DDX3X
IPI00218606 RPS23 40S ribosomal protein S23
IPI00026689 CDC2 Putative uncharacterized protein DKFZp686L20222
IPI00026314 GSN Isoform 1 of Gelsolin precursor
IPI00260090 ELMO2 Isoform 1 of Engulfment and cell motility protein 2
IPI00029054 NT5C2 Cytosolic purine 5-nucleotidase
IPI00179019 MRPL35 39S ribosomal protein L35, mitochondrial precursor
IPI00335589 RNMTL1 RNA methyltransferase-like protein 1
TIMM50 Isoform 2 of Import inner membrane translocase subunit TIM50,
IPI00418497 mitochondrial precursor
IPI00008524 PABPC1 Isoform 1 of Polyadenylate-binding protein 1
IPI00008433 RPS5 40S ribosomal protein S5
IPI00017963 SNRPD2 Small nuclear ribonucleoprotein Sm D2
IPI00221222 SUB1 Activated RNA polymerase II transcriptional coactivator p15
IPI00219153 RPL22 60S ribosomal protein L22
IPI00220740 NPM1 Isoform 2 of Nucleophosmin
IPI00000816 YWHAE 14-3-3 protein epsilon
IPI00182289 RPS29 40S ribosomal protein S29
IPI00017339 SF3B4 Splicing factor 3B subunit 4
IPI00005969 CAPZA1 F-actin-capping protein subunit alpha-1
IPI00744706 SPTAN1 282 kDa protein
IPI00003377 SFRS7 Isoform 1 of Splicing factor, arginine/serine-rich 7
ATP1A1 Isoform Long of Sodium/potassium-transporting ATPase subunit alpha-1
IPI00006482 precursor
IPI00479186 PKM2 Isoform M2 of Pyruvate kinase isozymes M1/M2
IPI00001639 KPNB1 Importin subunit beta-1
IPI00221093 RPS17 40S ribosomal protein S17
IPI00215884 SFRS1 Isoform ASF-1 of Splicing factor, arginine/serine-rich 1
IPI00186712 RPS26 OTTHUMP00000018641
IPI00008603 ACTA2 Actin, aortic smooth muscle
IPI00334627 ANXA2P2 Uncharacterized protein ENSP00000329640
IPI00376798 RPL11 Isoform 1 of 60S ribosomal protein L11
IPI00004839 CRKL Crk-like protein
IPI00011913 HNRNPA0 Heterogeneous nuclear ribonucleoprotein A0
IPI00010157 MAT2A S-adenosylmethionine synthetase isoform type-2
IPI00023503 CDK3 Cell division protein kinase 3
IPI00177817 ATP2A2 Isoform SERCA2A of Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
IPI00056494 RPL36AL 60S ribosomal protein L36a-like
IPI00022202 SLC25A3 Isoform A of Phosphate carrier protein, mitochondrial precursor
IPI00293845 RIF1 Isoform 1 of Telomere-associated protein RIF1
IPI00014301 OXA1L Oxidase (Cytochrome c) assembly 1-like
IPI00297779 CCT2 T-complex protein 1 subunit beta
IPI00103525 PSPC1 Isoform 1 of Paraspeckle component 1
HIST1H4E;HIST1H4A;HIST1H4I;HIST1H4B;HIST1H4F;HIST4H4;HIST1H4J;HIST1H4D;HIST
IPI00453473 2H4A;HIST2H4B;HIST1H4H;HI
IPI00027626 CCT6A T-complex protein 1 subunit zeta
IPI00015953 DDX21 Isoform 1 of Nucleolar RNA helicase 2
IPI00021840 RPS6 40S ribosomal protein S6
IPI00182533 RPL28 60S ribosomal protein L28
IPI00008438 RPS10 40S ribosomal protein S10
IPI00012772 RPL8 60S ribosomal protein L8
IPI00008530 RPLP0 60S acidic ribosomal protein P0
IPI00009865 KRT10 Keratin, type I cytoskeletal 10
IPI00021448 PIK3R1 Isoform 1 of Phosphatidylinositol 3-kinase regulatory subunit alpha
IPI00000073 EGF Pro-epidermal growth factor precursor
IPI00414860 RPL37A 60S ribosomal protein L37a
IPI00021435 PSMC2 26S protease regulatory subunit 7
LOC652595;SNRPA1 Novel protein similar to small nuclear ribonucleoprotein
IPI00183920 polypeptide A SNRPA1
IPI00168378 MTMR14 Isoform 1 of Myotubularin-related protein 14
IPI00745180 LOC285556 similar to hCG2038359
IPI00022018 DPM1 Dolichol-phosphate mannosyltransferase
IPI00032827 SF3B14 Pre-mRNA branch site protein p14
IPI00011200 PHGDH D-3-phosphoglycerate dehydrogenase
IPI00022320 MRPL42 Mitochondrial 28S ribosomal protein S32, mitochondrial precursor
IPI00291467 SLC25A6 ADP/ATP translocase 3
IPI00030363 ACAT1 Acetyl-CoA acetyltransferase, mitochondrial precursor
IPI00018140 SYNCRIP Isoform 1 of Heterogeneous nuclear ribonucleoprotein Q
IPI00376005 EIF5A Isoform 2 of Eukaryotic translation initiation factor 5A-1
IPI00010404 SF3B5 Splicing factor 3B subunit 5
IPI00410693 SERBP1 Isoform 1 of Plasminogen activator inhibitor 1 RNA-binding protein
IPI00021700 PCNA Proliferating cell nuclear antigen
IPI00002821 RPL14 60S ribosomal protein L14
IPI00018522 PRMT1 HMT1 hnRNP methyltransferase-like 2 isoform 1
IPI00219156 RPL30 60S ribosomal protein L30
IPI00012007 AHCY Adenosylhomocysteinase
IPI00007401 IPO8 Importin-8
IPI00465439 ALDOA Fructose-bisphosphate aldolase A
IPI00017964 SNRPD3 Small nuclear ribonucleoprotein Sm D3
IPI00301364 SKP1 Isoform 1 of S-phase kinase-associated protein 1
IPI00220808 PFKFB2 Isoform 2 of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
IPI00005614 SPTBN1 Isoform Long of Spectrin beta chain, brain 1
IPI00439415 EIF4B eukaryotic translation initiation factor 4B
IPI00010418 MYO1C Myosin-Ic
IPI00031388 PIK3CB Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform
IPI00008529 RPLP2 60S acidic ribosomal protein P2
IPI00005087 TMOD3 Tropomodulin-3
IPI00027547 DCD Dermcidin precursor
IPI00030910 CAPRIN1 Caprin-1
IPI00183526 NCL NCL protein
IPI00012268 PSMD2 26S proteasome non-ATPase regulatory subunit 2
IPI00219291 ATP5J2 Isoform 2 of ATP synthase subunit f, mitochondrial
IPI00027285 SNRPB Isoform SM-B of Small nuclear ribonucleoprotein-associated proteins B and B
LOC387867 similar to 40S ribosomal protein SA (p40) (34/67 kDa laminin receptor)
IPI00398958 (Colon carcinoma la
IPI00395769 ATP5C1 Isoform Heart of ATP synthase subunit gamma, mitochondrial precursor
IPI00027350 PRDX2 Peroxiredoxin-2
IPI00032003 EMD Emerin
IPI00643041 RAN GTP-binding nuclear protein Ran
IPI00017617 DDX5 Probable ATP-dependent RNA helicase DDX5
IPI00021187 RUVBL1 Isoform 1 of RuvB-like 1
IPI00004399 ERRFI1 ERBB receptor feedback inhibitor 1
IPI00022462 TFRC Transferrin receptor protein 1
IPI00247583 LOC729402;RPL21 60S ribosomal protein L21
IPI00022694 PSMD4 Isoform Rpn10A of 26S proteasome non-ATPase regulatory subunit 4
IPI00220327 KRT1 Keratin, type II cytoskeletal 1
IPI00399212 LOC389842 similar to RanBP1
IPI00333010 CHERP Isoform 1 of Calcium homeostasis endoplasmic reticulum protein
IPI00456359 ATXN2L Isoform 1 of Ataxin-2-like protein
IPI00006932 LUC7L2 Isoform 1 of Putative RNA-binding protein Luc7-like 2
IPI00022434 ALB Uncharacterized protein ALB
IPI00030320 DDX6 Probable ATP-dependent RNA helicase DDX6
IPI00000690 AIFM1 Isoform 1 of Apoptosis-inducing factor 1, mitochondrial precursor
C1QBP Complement component 1 Q subcomponent-binding protein, mitochondrial
IPI00014230 precursor
IPI00179187 DNAJA3 Isoform 2 of DnaJ homolog subfamily A member 3, mitochondrial precursor
IPI00026781 FASN Fatty acid synthase
IPI00159899 ANKFY1 Similar to Ankyrin repeat and FYVE domain-containing protein 1
IPI00176574 LOC284230 similar to mCG7611
IPI00186290 EEF2 Elongation factor 2
IPI00217223 PAICS Multifunctional protein ADE2
IPI00030131 TMPO Isoform Beta of Lamina-associated polypeptide 2, isoforms beta/gamma
IPI00396378 HNRNPA2B1 Isoform B1 of Heterogeneous nuclear ribonucleoproteins A2/B1
MAP3K7IP3 Isoform 1 of Mitogen-activated protein kinase kinase kinase 7-interacting
IPI00166840 protein 3
IPI00061178 CCBL2 kynurenine aminotransferase III isoform 3
IPI00410590 LSM14A Isoform 2 of LSM14 protein homolog A
IPI00719622 RPS28;LOC646195;LOC645899 40S ribosomal protein S28
IPI00219155 RPL27 60S ribosomal protein L27
IPI00017334 PHB Prohibitin
IPI00060650 PCNP Isoform 2 of PEST proteolytic signal-containing nuclear protein
IPI00003935 HIST2H2BE Histone H2B type 2-E
IPI00004344 AFF4 Isoform 1 of AF4/FMR2 family member 4
IPI00011126 PSMC1 26S protease regulatory subunit 4
IPI00385001 NUP188 Isoform 2 of Nucleoporin NUP188 homolog
IPI00176702 KIAA1543 Uncharacterized protein KIAA1543
IPI00014319 IVNS1ABP Influenza virus NS1A-binding protein
IPI00419585 PPIA;PPIAL3;LOC654188 Peptidyl-prolyl cis-trans isomerase A
IPI00018465 CCT7 T-complex protein 1 subunit eta
IPI00292135 LBR Lamin-B receptor
IPI00301263 CAD CAD protein
IPI00017510 MT-CO2 Cytochrome c oxidase subunit 2
IPI00011770 NDUFA4 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4
IPI00024145 VDAC2 Voltage-dependent anion-selective channel protein 2
IPI00008240 MARS Methionyl-tRNA synthetase, cytoplasmic
IPI00306290 XPOT Exportin-T
IPI00005511 PHF5A PHD finger-like domain-containing protein 5A
IPI00304935 SAAL1 Protein SAAL1
IPI00009104 RUVBL2 RuvB-like 2
IPI00302850 SNRPD1 Small nuclear ribonucleoprotein Sm D1
RPN1 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
IPI00025874 precursor
IPI00064765 RPL10L 60S ribosomal protein L10-like
IPI00025491 EIF4A1 Eukaryotic initiation factor 4A-I
IPI00024650 SLC16A1 Monocarboxylate transporter 1
IPI00641950 GNB2L1 Lung cancer oncogene 7
IPI00478834 ARGLU1 Isoform 1 of Arginine and glutamate-rich protein 1
IPI00008454 DNAJB11 DnaJ homolog subfamily B member 11 precursor
IPI00031370 TUBB2B Tubulin beta-2B chain
IPI00012074 HNRNPR Heterogeneous nuclear ribonucleoprotein R
IPI00019472 SLC1A5 Neutral amino acid transporter B
IPI00100160 CAND1 Isoform 1 of Cullin-associated NEDD8-dissociated protein 1
IPI00021263 YWHAZ 14-3-3 protein zeta/delta
IPI00006980 C14orf166 Protein C14orf166
IPI00220528 SNRPF Small nuclear ribonucleoprotein F
IPI00024364 TNPO1 transportin 1 isoform 1
IPI00031820 FARSA Phenylalanyl-tRNA synthetase alpha chain
IPI00413755 TAF4 Transcription initiation factor TFIID subunit 4
IPI00176706 RBM17 Splicing factor 45
DBT Lipoamide acyltransferase component of branched-chain alpha-keto acid
IPI00003944 dehydrogenase complex, mi
IPI00010841 RHOD Rho-related GTP-binding protein RhoD precursor
IPI00018627 NAT13 Isoform 1 of N-acetyltransferase 13
IPI00386010 MRPL12 5C5
LTBP4 Isoform 4 of Latent-transforming growth factor beta-binding protein 4
IPI00395783 precursor
IPI00640703 XPO5 Isoform 1 of Exportin-5
IPI00021304 KRT2 Keratin, type II cytoskeletal 2 epidermal
IPI00218414 CA2 Carbonic anhydrase 2
IPI00012535 DNAJA1 DnaJ homolog subfamily A member 1
IPI00002790 SEL1L Isoform 1 of Protein sel-1 homolog 1 precursor
IPI00029628 RCN2 Reticulocalbin-2 precursor
IPI00414604 E2F7 Isoform 1 of Transcription factor E2F7
IPI00374260 LOC284393 similar to QM protein isoform 1
IPI00219729 SLC25A11 Mitochondrial 2-oxoglutarate/malate carrier protein
IPI00293464 DDB1 DNA damage-binding protein 1
MAP3K7IP2 Isoform 1 of Mitogen-activated protein kinase kinase kinase 7-interacting
IPI00419504 protein 2
IPI00004968 PRPF19 Pre-mRNA-processing factor 19
IPI00016610 PCBP1 Poly(rC)-binding protein 1
IPI00025050 WARS2 Tryptophanyl-tRNA synthetase, mitochondrial precursor
IPI00020134 SOS2 Son of sevenless homolog 2
IPI00299904 MCM7 Isoform 1 of DNA replication licensing factor MCM7
IPI00029744 SSBP1 Single-stranded DNA-binding protein, mitochondrial precursor
IPI00022334 OAT Ornithine aminotransferase, mitochondrial precursor
IPI00290142 CTPS CTP synthase 1
IPI00107745 CROP Isoform 1 of Cisplatin resistance-associated overexpressed protein
IPI00045921 ATAD3B TOB3
IPI00007208 DDX41 Probable ATP-dependent RNA helicase DDX41
IPI00219740 MCM7 Isoform 2 of DNA replication licensing factor MCM7
IPI00030274 CCDC55 Coiled-coil domain-containing protein 55
IPI00025273 GART Isoform Long of Trifunctional purine biosynthetic protein adenosine-3
IPI00019906 BSG Isoform 2 of Basigin precursor
IPI00013415 RPS7 40S ribosomal protein S7
IPI00005589 hCG_18290 Uncharacterized protein ENSP00000275524
IPI00418248 HCCA2 Isoform 1 of Mps one binder kinase activator-like 2
IPI00012998 CPSF6 Isoform 1 of Cleavage and polyadenylation specificity factor subunit 6
PPP1CC Isoform Gamma-1 of Serine/threonine-protein phosphatase PP1-gamma
IPI00005705 catalytic subunit
IPI00163505 RBM39 Isoform 1 of RNA-binding protein 39
IPI00219160 RPL34 60S ribosomal protein L34
IPI00853278 SLAIN2 SLAIN motif-containing protein 2
IPI00017451 SF3A1 Splicing factor 3 subunit 1
OGT Isoform 3 of UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase
IPI00005780 110 kDa subunit
IPI00001636 ATXN10 Ataxin-10
IPI00009867 KRT5 Keratin, type II cytoskeletal 5
IPI00007084 SLC25A13 Mitochondrial aspartate-glutamate carrier protein
CHCHD3 Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial
IPI00015833 precursor
IPI00016613 CSNK2A1 CSNK2A1 protein
IPI00018873 PBEF1 Isoform 1 of Nicotinamide phosphoribosyltransferase
IPI00019385 SSR4 Translocon-associated protein subunit delta precursor
IPI00022774 VCP Transitional endoplasmic reticulum ATPase
IPI00106567 WDR33 WD repeat-containing protein 33
IPI00217966 LDHA Isoform 1 of L-lactate dehydrogenase A chain
IPI00218493 HPRT1 Hypoxanthine-guanine phosphoribosyltransferase
IPI00219330 ILF3 Isoform 5 of Interleukin enhancer-binding factor 3
IPI00219616 PRPS1 Ribose-phosphate pyrophosphokinase 1
IPI00294159 SLC25A1 Tricarboxylate transport protein, mitochondrial precursor
IPI00295940 UNC84B Protein unc-84 homolog B
IPI00300127 NAT10 N-acetyltransferase 10
IPI00301503 SFRS10 Isoform 1 of Splicing factor, arginine/serine-rich 10
IPI00306369 NSUN2 tRNA
IPI00337315 RBBP6 Isoform 1 of Retinoblastoma-binding protein 6
IPI00374732 PPIAP19 similar to peptidylprolyl isomerase A-like
IPI00401264 TXNDC4 Thioredoxin domain-containing protein 4 precursor
IPI00419373 HNRPA3 Isoform 1 of Heterogeneous nuclear ribonucleoprotein A3
IPI00783313 PYGL Glycogen phosphorylase, liver form
IPI00440688 POLDIP3 Isoform 1 of Polymerase delta-interacting protein 3
IPI00031420 UGDH UDP-glucose 6-dehydrogenase
IPI00328319 RBBP4 Histone-binding protein RBBP4
IPI00007334 ACIN1 Isoform 1 of Apoptotic chromatin condensation inducer in the nucleus
IPI00024919 PRDX3 Thioredoxin-dependent peroxide reductase, mitochondrial precursor
IPI00303753 C1orf163 Hcp beta-lactamase-like protein C1orf163
IPI00008449 FIP1L1 Isoform 3 of Pre-mRNA 3-end-processing factor FIP1
IPI00017341 SF3A2 SF3A2 protein (Fragment)
IPI00002349 NUFIP2 Nuclear fragile X mental retardation-interacting protein 2
IPI00027834 HNRNPL heterogeneous nuclear ribonucleoprotein L isoform a
IPI00301395 CPVL Probable serine carboxypeptidase CPVL precursor
IPI00009960 IMMT Isoform 1 of Mitochondrial inner membrane protein
IPI00298961 XPO1 Exportin-1
IPI00026216 NPEPPS Puromycin-sensitive aminopeptidase
IPI00005745 SPTLC1 Serine palmitoyltransferase 1
IPI00022744 CSE1L Isoform 1 of Exportin-2
IPI00301434 BOLA2B;BOLA2 BolA-like protein 2
NDUFS3 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
IPI00025796 precursor
IPI00395887 TXNDC1 Thioredoxin domain-containing protein 1 precursor
IPI00305383 UQCRC2 Cytochrome b-c1 complex subunit 2, mitochondrial precursor
IPI00024067 CLTC Isoform 1 of Clathrin heavy chain 1
IPI00024976 TOMM22 Mitochondrial import receptor subunit TOM22 homolog
IPI00008998 PTPLAD1 Protein tyrosine phosphatase-like protein PTPLAD1
IPI00179964 PTBP1 Isoform 1 of Polypyrimidine tract-binding protein 1
IPI00456898 LOC440055, UNCHARACTERIZED PROTEIN
IPI00182757 KIAA1967 Isoform 1 of Protein KIAA1967
IPI00175151 TET2 Isoform 1 of Uncharacterized protein KIAA1546
PPP2R1A cDNA FLJ34068 fis, clone FCBBF3001918, highly similar to
IPI00168184 SERINE/THREONINE PROTEIN PHOSPHATA
IPI00019359 KRT9 Keratin, type I cytoskeletal 9
IPI00029267 SNRPB2 U2 small nuclear ribonucleoprotein B
(Cont) and after (EGFR) EGF stimulation, and EGFR mutants (Y998F and S991A) after EGF stimulation. Proteins were identified by LTQ-O
bability, 2) more than 95% protein identification probability, 3) more than 2 unique peptides per protein in either samples. The total assi
ng to the assigned peptide number in EGFR sample. Assigned peptide number were total peptides obtained by Scaffold analysis from 4
of unique spectra and protein identification probability were from one of 4 experiments when that protein had the highest percentage seq
Spectral Counts (normalized to EGFR) Protein Identification
Percentage Number of
sequence unique
MW Cont EGFR Y998F S991A coverage peptides
134245 892 892 892 892 71.40% 72
(1131) (892) (1127) (1284)
32 69 57 51
50583 281 354 295 254 45.00% 17
70036 172 186 158 152 58.30% 28
99631 42 172 95 54 44.20% 30
49653 153 151 165 161 72.70% 22
71304 138 141 163 146 51.60% 29
25189 77 139 104 75 71.90% 15
69209 90 136 121 102 36.20% 23
152451 107 133 68 37 51.40% 60
98147 107 117 105 95 33.80% 26
41720 79 111 64 59 64.80% 14
48101 108 108 84 67 66.70% 24
108651 84 105 141 109 30.80% 30
110322 70 101 85 67 28.70% 23
54174 74 98 89 78 50.80% 18
36705 95 97 125 102 79.20% 17
44579 88 93 72 50 43.20% 17
25366 70 78 69 48 52.70% 9
33404 75 76 60 51 63.20% 16
13165 50 75 49 39 16.50% 2
62876 27 74 46 23 43.50% 17
83249 71 74 61 52 36.60% 16
22093 63 73 65 50 69.80% 13
49907 78 71 83 75 46.20% 15
270602 57 69 64 60 18.40% 30
70882 64 68 72 61 42.00% 20
37518 70 67 64 50 43.10% 14
50961 59 66 57 48 40.60% 16
65946 50 63 36 25 30.70% 15
34902 65 62 45 36 61.40% 13
36892 50 60 43 38 47.70% 13
20666 51 60 47 37 59.90% 12
40451 68 60 58 45 45.60% 15
24897 58 58 50 41 55.60% 10
33518 58 57 48 37 42.80% 12
50348 51 56 49 36 42.60% 15
26671 47 55 33 24 44.00% 13
52624 37 55 25 10 57.60% 12
29561 52 55 40 32 47.20% 15
35906 54 54 38 32 60.50% 11
20675 48 52 44 30 69.10% 10
38615 57 51 45 39 44.00% 12
23613 51 51 40 34 50.00% 11
35333 58 50 47 33 52.30% 14
20033 48 50 39 41 61.70% 12
30167 46 50 39 30 37.80% 8
54626 39 49 41 30 43.20% 8
23624 44 49 39 30 24.70% 19
103694 46 49 22 9 43.30% 14
28432 37 48 32 24 37.10% 8
279991 61 48 101 98 23.00% 11
88962 38 48 39 30 26.10% 48
27988 37 47 28 30 54.10% 10
47852 54 47 50 50 42.60% 12
41019 34 47 37 31 51.70% 13
55439 40 45 39 32 41.50% 13
65566 33 45 47 47 33.60% 16
21330 36 44 30 25 47.00% 7
106107 49 43 71 58 38.40% 19
29467 34 43 27 20 27.60% 22
73663 44 43 50 39 52.10% 10
17701 25 42 24 20 44.10% 8
30140 50 41 32 27 46.10% 10
22167 41 41 37 32 38.80% 8
97017 12 41 7 3 24.00% 18
23142 41 40 29 23 53.60% 10
124027 28 39 34 32 17.60% 13
64212 32 38 31 21 28.50% 12
17425 37 37 26 22 40.90% 7
24474 38 37 32 23 41.70% 8
76132 32 36 33 31 31.60% 10
49212 25 36 36 32 35.50% 14
54214 41 36 39 34 24.20% 11
72406 50 35 40 34 47.50% 23
18307 49 35 35 27 67.10% 9
100211 24 34 28 27 18.30% 12
59734 40 33 34 32 32.90% 15
67552 22 32 32 26 23.80% 11
59592 27 32 17 9 20.40% 11
33283 36 31 26 19 36.40% 8
44450 25 31 30 27 30.70% 8
23918 26 30 21 16 26.40% 5
17763 29 30 27 23 40.50% 7
58045 30 30 13 10 34.50% 14
36725 28 29 30 28 47.50% 6
28657 29 29 24 21 29.50% 6
32879 32 29 33 30 42.60% 11
15365 27 29 23 21 62.80% 6
20558 25 28 24 15 30.60% 5
31688 30 28 26 23 50.20% 11
29583 25 28 21 20 48.10% 8
16428 21 28 17 13 41.80% 7
53987 18 26 25 25 35.60% 12
49858 38 26 35 35 28.00% 9
26197 15 25 26 20 41.20% 6
16055 20 25 18 17 72.30% 6
12089 26 25 18 18 72.60% 5
49813 23 25 25 26 44.30% 6
90917 17 24 20 17 39.00% 11
42917 29 24 12 10 52.40% 9
30290 36 24 16 17 36.50% 9
29581 26 24 23 19 28.70% 9
57209 24 24 27 20 11.70% 8
17678 20 23 16 14 50.00% 6
145802 19 23 34 29 42.30% 6
19180 26 23 22 14 41.80% 10
22575 18 23 19 15 17.90% 16
469078 49 22 46 28 12.60% 43
36035 21 22 24 19 38.20% 8
22896 20 22 19 13 22.20% 4
29927 19 22 12 8 28.40% 7
24244 17 21 13 10 42.30% 7
24188 20 21 20 16 18.50% 8
60327 17 21 6 7 34.10% 7
11949 14 21 20 17 65.00% 5
21348 14 20 13 8 41.40% 5
16043 9 20 16 12 38.60% 5
15930 18 20 9 12 37.70% 5
14848 18 20 20 18 20.20% 6
16255 14 20 13 10 36.60% 6
18885 22 19 17 12 29.30% 4
60446 30 19 18 16 28.10% 12
25018 21 19 18 11 38.40% 7
13725 11 17 12 7 24.10% 4
21821 16 17 13 11 22.30% 3
16604 18 17 14 14 37.00% 7
23755 12 17 12 11 21.20% 5
31307 13 17 11 9 17.90% 10
109435 8 17 26 17 36.30% 6
17762 14 17 9 8 24.00% 4
29978 6 16 7 5 41.50% 2
17948 11 16 8 8 47.50% 9
13646 18 16 15 13 21.80% 9
18413 17 16 15 14 22.10% 9
63438 21 16 7 9 25.00% 2
54152 21 16 11 11 11.50% 10
10147 13 16 8 10 26.70% 6
135561 19 16 21 14 36.50% 4
66201 21 15 5 3 22.20% 8
14445 10 15 10 10 15.70% 7
17205 15 15 9 9 30.10% 12
57908 14 15 11 5 51.50% 6
61038 20 15 11 9 18.40% 5
56543 23 15 18 21 48.60% 3
14822 13 15 12 9 34.60% 3
23449 13 15 11 8 25.00% 9
15802 13 15 13 10 32.80% 4
12241 17 15 20 14 33.10% 5
59654 24 15 17 15 14.30% 6
17238 13 15 8 7 24.10% 4
40432 10 14 8 8 30.20% 2
24633 24 14 6 10 70.50% 12
11719 16 14 19 12 57.10% 6
15078 18 14 9 10 22.70% 3
222149 13 14 2 2 8.78% 14
27747 19 14 12 11 28.60% 7
17801 9 13 8 7 34.10% 5
116188 5 13 5 6 48.80% 5
40071 10 13 10 6 37.60% 4
18485 12 13 9 8 6.26% 6
133264 10 12 16 21 9.71% 8
38417 16 12 13 12 24.50% 7
30683 13 12 10 8 22.70% 4
9443 11 12 13 12 39.30% 3
22331 10 12 9 10 19.80% 4
15406 7 12 7 8 28.60% 3
81608 0 12 3 2 17.20% 9
13061 10 11 10 10 19.60% 3
12236 9 11 7 3 10.60% 5
17023 9 11 6 6 17.40% 5
21379 13 11 7 9 13.30% 2
59461 10 11 7 6 28.60% 3
32711 10 11 5 6 40.70% 3
118278 11 10 17 10 10.40% 5
59534 13 10 1 1 18.00% 5
38205 12 10 9 6 18.80% 6
50101 13 10 10 6 21.60% 12
72679 10 10 17 10 6.81% 5
90965 9 10 7 8 26.90% 2
14118 8 10 8 6 21.50% 6
38828 13 10 9 5 11.60% 9
14535 7 9 6 5 8.10% 9
26540 2 9 5 5 38.50% 2
164873 4 9 17 17 7.67% 9
7602 6 9 8 6 50.00% 3
170632 17 9 2 2 22.70% 2
8201 9 9 7 8 6.90% 3
13355 9 9 6 3 10.40% 5
58112 6 9 6 3 23.80% 3
73228 12 9 13 6 31.40% 6
15790 10 9 9 8 6.78% 4
34762 6 9 9 10 26.00% 3
85680 0 9 0 1 13.50% 7
82601 6 9 2 0 7.29% 2
64955 27 8 1 1 27.10% 11
21497 7 8 6 5 13.30% 3
47003 10 8 2 1 11.90% 4
50448 12 8 6 8 11.20% 4
70653 13 8 8 5 12.60% 6
22859 9 8 7 6 26.50% 4
13509 9 8 13 10 40.70% 4
14378 5 8 5 4 26.00% 3
14769 6 7 6 4 4.77% 8
29447 6 7 5 6 21.40% 5
29157 6 7 9 7 11.90% 9
6659 3 7 6 3 36.70% 3
44369 4 7 6 4 10.70% 7
32905 0 7 2 1 40.00% 3
282132 5 7 16 13 29.80% 7
27350 7 7 11 6 20.90% 2
112882 13 7 12 14 24.90% 3
57920 6 7 2 1 10.90% 3
97153 10 7 12 12 17.40% 3
15533 8 7 7 6 18.80% 2
27727 13 7 16 11 16.60% 3
12983 5 7 4 2 23.90% 3
41992 8 7 8 5 26.80% 2
38643 3 7 5 5 13.00% 3
20235 7 7 6 5 15.00% 3
33759 4 7 2 0 29.40% 7
30823 5 6 4 3 5.71% 2
43643 2 6 2 2 7.82% 7
37597 6 6 2 3 23.60% 3
109675 7 6 7 8 23.80% 9
12451 6 6 4 3 3.44% 5
40078 6 6 10 7 13.70% 5
274451 4 6 8 10 9.91% 4
55369 10 6 9 8 14.70% 5
57472 7 6 8 4 38.80% 4
58727 4 6 14 11 5.84% 3
11350 6 6 3 5 11.10% 6
58007 5 6 3 3 9.51% 2
87328 5 6 4 4 21.70% 4
28664 6 6 4 4 22.60% 4
15730 6 6 4 3 23.00% 3
18880 8 6 8 6 10.50% 2
28007 4 6 2 1 14.20% 4
34256 5 6 3 3 9.87% 3
59494 0 6 14 9 27.50% 15
83583 1 6 0 1 9.81% 5
133928 0 6 11 7 3.40% 2
10257 3 5 2 1 1.26% 2
48618 0 5 1 1 14.60% 3
28386 2 5 3 1 17.60% 2
72188 2 5 5 5 17.40% 8
193650 6 5 6 6 52.80% 4
29618 8 5 4 2 37.60% 2
14568 1 5 2 1 15.90% 5
56633 10 5 17 14 8.83% 4
16643 11 5 6 5 38.60% 5
32849 6 5 6 7 43.00% 3
45182 8 5 4 9 15.90% 4
69586 9 5 2 3 33.70% 6
20153 10 5 8 7 10.80% 2
10118 5 5 4 5 15.90% 4
44948 5 5 6 4 40.90% 3
28751 8 5 12 13 5.56% 2
23272 6 5 2 3 4.05% 3
42444 9 5 8 3 8.52% 3
12767 4 5 5 3 24.60% 2
47699 5 5 3 2 17.80% 3
119960 3 5 5 5 10.60% 2
39403 6 5 3 6 28.30% 2
13899 4 5 9 7 2.37% 4
18640 4 5 2 2 35.50% 3
54389 4 5 1 0 5.31% 2
274595 1 5 7 5 12.20% 2
69136 3 5 5 5 8.00% 3
118024 2 5 1 1 1.75% 2
122749 0 5 2 2 3.36% 3
11647 1 4 2 1 3.85% 2
39578 1 4 0 0 3.70% 2
11266 0 4 0 0 20.50% 2
72732 3 4 2 0 27.30% 2
50934 2 4 4 1 13.30% 4
100184 3 4 3 1 7.12% 2
10346 6 4 6 6 15.20% 4
24593 5 4 10 8 19.70% 2
32726 2 4 2 2 16.90% 3
32865 7 4 5 3 21.30% 4
21874 5 4 3 3 6.03% 3
28977 6 4 4 3 14.50% 5
24405 9 4 9 7 22.30% 6
69132 4 4 2 1 3.95% 2
50211 2 4 9 8 20.60% 2
50543 7 4 6 4 10.90% 3
84856 4 4 0 2 45.20% 2
18547 4 4 2 1 26.60% 16
40719 6 4 6 3 7.10% 2
66001 0 4 22 10 20.00% 2
23334 4 3 1 1 9.44% 3
103556 4 3 6 3 6.86% 4
113355 2 3 2 3 7.25% 2
46497 5 3 4 4 10.40% 6
71687 13 3 3 3 27.00% 4
54401 2 3 4 3 10.20% 3
66884 7 3 6 1 1.27% 2
31345 8 3 11 9 4.71% 3
49643 8 3 9 8 9.95% 2
273382 5 3 2 1 25.50% 2
132806 2 3 1 0 7.34% 5
12320 3 3 1 1 4.88% 2
95322 10 3 6 7 7.05% 2
49662 4 3 2 2 7.37% 2
50653 5 3 6 3 5.46% 2
37412 2 3 2 1 10.40% 5
78635 3 3 6 3 4.36% 2
42125 2 3 2 0 6.05% 3
50537 1 3 7 4 46.40% 3
7823 4 3 9 5 12.50% 2
15780 4 3 1 1 16.20% 2
29787 2 3 2 1 4.56% 3
13527 0 3 6 3 23.80% 2
13903 2 2 2 1 19.00% 2
127444 0 2 0 1 3.70% 2
49168 0 2 1 0 6.14% 2
182280 2 2 2 1 8.26% 4
134734 2 2 4 2 3.52% 2
71712 0 2 4 5 2.56% 3
17995 2 2 2 3 21.20% 2
59350 6 2 7 6 8.84% 3
70688 8 2 3 1 6.02% 3
242965 2 2 3 2 1.03% 2
25548 4 2 0 0 8.81% 2
9352 3 2 4 2 27.20% 2
38076 1 2 2 1 9.22% 2
101100 6 2 3 6 3.67% 3
109949 4 2 1 1 3.22% 2
12387 4 2 6 3 32.70% 3
53638 5 2 1 0 7.58% 3
51140 5 2 5 5 8.21% 3
13264 2 2 5 4 27.70% 2
72762 2 2 2 3 5.73% 2
24501 6 2 1 2 9.81% 2
46137 3 2 2 1 5.67% 2
53942 8 2 6 8 10.80% 5
37871 7 2 1 1 13.30% 3
33199 3 2 4 1 5.86% 2
40497 3 2 9 9 14.20% 4
49935 8 2 2 1 54.40% 2
70926 2 2 2 0 7.11% 2
56582 3 2 2 2 7.58% 3
136363 1 2 2 3 2.36% 3
27728 3 2 4 2 14.70% 2
28051 1 2 4 3 18.90% 3
9707 2 2 2 3 24.40% 2
102341 2 2 2 2 3.67% 3
57547 5 2 3 3 4.92% 2
110096 1 2 2 2 2.40% 2
44944 2 2 6 3 16.70% 3
53471 2 1 2 1 5.04% 2
23447 2 1 1 0 7.26% 3
19381 2 1 0 0 28.60% 2
19527 2 1 1 3 18.10% 4
142530 0 1 2 2 13.60% 2
136297 2 1 1 1 22.80% 11
65848 0 1 9 3 17.00% 3
29229 1 1 2 1 8.46% 2
44851 2 1 2 4 19.60% 2
88739 0 1 2 3 24.70% 2
36860 2 1 4 3 4.64% 2
99828 2 1 8 5 6.81% 5
24610 3 1 2 0 1.66% 2
34045 4 1 4 4 7.64% 2
126952 6 1 0 0 6.84% 7
76476 2 1 0 0 6.49% 2
55163 3 1 0 1 5.75% 2
37480 5 1 4 2 10.70% 2
40129 2 1 5 3 10.30% 3
153019 5 1 5 3 11.00% 6
81291 1 1 0 3 7.65% 2
17242 1 1 3 1 22.30% 2
48518 3 1 6 5 10.50% 3
66673 3 1 6 5 8.80% 4
51449 2 1 3 2 6.71% 2
65099 3 1 2 1 6.75% 2
69822 3 1 2 4 6.43% 3
44630 3 1 3 4 10.30% 2
66373 3 1 5 3 6.45% 3
107750 3 1 9 8 6.73% 4
29203 4 1 2 5 15.20% 3
22110 5 1 8 5 27.30% 4
15733 4 1 1 1 17.00% 2
26909 2 1 2 2 7.59% 2
59193 0 1 4 1 5.44% 2
36968 3 1 5 2 8.05% 2
59363 6 1 7 8 11.90% 5
13275 2 1 2 1 15.40% 2
62527 1 1 2 0 5.34% 2
88868 0 1 11 3 11.00% 7
116910 1 1 2 3 2.49% 2
53473 0 0 0 3 7.16% 2
62362 0 0 2 0 3.88% 2
74287 0 0 2 1 5.33% 3
26135 1 0 1 1 2.91% 3
45892 2 0 2 0 6.15% 2
55505 0 0 2 1 10.80% 2
20195 0 0 2 2 7.49% 2
89307 0 0 2 1 9.82% 2
145903 2 0 2 1 4.57% 2
36671 1 0 0 1 7.13% 2
24562 2 0 2 1 16.30% 2
74590 2 0 0 1 2.85% 2
34817 0 0 3 1 8.98% 2
33995 0 0 2 1 3.85% 2
80294 0 0 3 1 2.17% 2
115690 2 0 0 0 6.63% 2
33649 0 0 3 0 10.60% 2
86455 2 0 0 1 3.04% 2
201551 0 0 0 1 13.50% 3
23972 2 0 2 0 7.40% 2
46955 0 0 2 0 2.79% 2
39577 2 0 0 0 4.68% 2
97134 0 0 2 2 12.20% 2
46072 0 0 7 1 9.96% 2
55007 1 0 0 2 3.78% 2
47638 1 0 2 0 5.65% 2
151870 1 0 6 3 1.51% 2
27675 0 0 2 1 11.00% 2
25719 4 0 1 1 8.62% 2
58358 0 0 3 0 7.14% 2
51457 0 0 2 2 11.90% 3
76103 1 0 2 0 4.96% 3
64115 5 0 4 3 8.15% 4
54148 0 0 2 0 4.83% 2
83661 4 0 4 3 6.07% 3
123371 6 0 9 6 4.48% 3
103261 2 0 1 4 2.72% 2
52727 3 0 1 0 4.86% 2
110404 2 0 0 2 1.85% 2
16914 3 0 7 5 28.90% 3
30224 0 0 2 0 12.10% 2
31774 1 0 3 1 11.80% 3
48425 3 0 1 3 10.60% 3
191601 2 0 2 1 1.25% 2
15504 2 0 2 3 16.20% 2
44251 2 0 1 3 8.60% 2
57205 5 0 1 1 9.60% 3
14580 9 0 7 3 45.50% 4
102885 2 0 0 0 2.82% 2
223800 1 0 3 3 2.35% 2
56832 0 0 0 2 3.54% 2
62113 2 0 6 3 12.80% 6
25470 0 0 2 0 8.44% 2
oteins were identified by LTQ-Orbitrap
n either samples. The total assigned
d by Scaffold analysis from 4
had the highest percentage sequence
Protein Identification
Number of Protein
unique identification
spectra probability
110 100.00%
25 100.00%
40 100.00%
35 100.00%
28 100.00%
41 100.00%
20 100.00%
32 100.00%
71 100.00%
29 100.00%
16 100.00%
29 100.00%
39 100.00%
25 100.00%
25 100.00%
23 100.00%
21 100.00%
13 100.00%
18 100.00%
2 99.00%
19 100.00%
19 100.00%
15 100.00%
23 100.00%
31 100.00%
25 100.00%
18 100.00%
16 100.00%
19 100.00%
18 100.00%
15 100.00%
12 100.00%
16 100.00%
11 100.00%
14 100.00%
17 100.00%
14 100.00%
12 100.00%
16 100.00%
11 100.00%
13 100.00%
15 100.00%
13 100.00%
16 100.00%
13 100.00%
11 100.00%
8 100.00%
19 100.00%
14 100.00%
8 100.00%
11 100.00%
48 100.00%
11 100.00%
14 100.00%
15 100.00%
16 100.00%
18 100.00%
8 100.00%
20 100.00%
26 100.00%
13 100.00%
8 100.00%
12 100.00%
10 100.00%
19 100.00%
12 100.00%
13 100.00%
12 100.00%
8 100.00%
11 100.00%
12 100.00%
14 100.00%
12 100.00%
25 100.00%
11 100.00%
12 100.00%
15 100.00%
11 100.00%
13 100.00%
8 100.00%
10 100.00%
7 100.00%
8 100.00%
14 100.00%
7 100.00%
7 100.00%
11 100.00%
7 100.00%
6 100.00%
12 100.00%
9 100.00%
8 100.00%
12 100.00%
10 100.00%
7 100.00%
8 100.00%
6 100.00%
6 100.00%
11 100.00%
11 100.00%
9 100.00%
9 100.00%
8 100.00%
6 100.00%
6 100.00%
10 100.00%
17 100.00%
44 100.00%
8 100.00%
5 100.00%
8 100.00%
7 100.00%
8 100.00%
8 100.00%
5 100.00%
6 100.00%
5 100.00%
5 100.00%
7 100.00%
6 100.00%
7 100.00%
12 100.00%
8 100.00%
5 100.00%
3 99.90%
7 100.00%
5 100.00%
10 100.00%
6 100.00%
4 100.00%
3 99.00%
9 100.00%
9 100.00%
10 100.00%
2 99.40%
10 100.00%
6 100.00%
4 100.00%
8 100.00%
7 100.00%
13 100.00%
6 100.00%
5 100.00%
3 99.90%
3 99.90%
9 100.00%
5 100.00%
5 100.00%
6 100.00%
4 100.00%
2 99.00%
16 100.00%
6 100.00%
4 99.90%
14 100.00%
7 100.00%
5 100.00%
5 100.00%
4 100.00%
6 100.00%
8 100.00%
8 100.00%
4 100.00%
3 99.90%
4 100.00%
3 99.90%
9 100.00%
4 99.90%
5 100.00%
5 100.00%
3 99.00%
3 99.90%
3 99.90%
5 100.00%
5 100.00%
6 100.00%
12 100.00%
5 100.00%
3 99.00%
6 100.00%
9 100.00%
9 100.00%
3 99.00%
9 100.00%
3 99.90%
2 99.00%
3 99.90%
5 100.00%
3 99.90%
7 100.00%
4 100.00%
3 99.90%
8 100.00%
2 99.00%
12 100.00%
3 100.00%
4 100.00%
4 100.00%
6 100.00%
4 100.00%
4 100.00%
3 99.90%
8 100.00%
5 100.00%
9 100.00%
3 99.90%
8 100.00%
4 99.90%
8 100.00%
2 99.00%
4 99.90%
4 99.90%
3 99.90%
2 99.00%
4 99.90%
3 99.90%
2 99.00%
3 99.90%
3 99.90%
7 100.00%
2 99.00%
7 100.00%
3 99.90%
9 100.00%
5 100.00%
5 100.00%
4 100.00%
5 100.00%
4 100.00%
3 99.90%
6 100.00%
2 99.00%
4 100.00%
4 100.00%
3 99.90%
2 99.50%
4 100.00%
3 99.90%
16 100.00%
5 100.00%
2 99.00%
2 99.00%
3 99.90%
2 99.00%
8 100.00%
5 100.00%
2 99.00%
5 100.00%
4 100.00%
5 100.00%
3 99.90%
4 100.00%
6 100.00%
2 99.00%
4 100.00%
3 99.90%
2 99.00%
3 99.90%
3 99.90%
2 99.00%
3 99.90%
2 99.40%
2 99.00%
4 100.00%
4 99.90%
2 99.00%
2 99.00%
3 99.90%
2 99.00%
3 100.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
4 100.00%
2 99.00%
4 100.00%
2 99.00%
3 99.90%
4 100.00%
3 99.90%
5 100.00%
6 100.00%
2 99.00%
2 99.70%
3 99.90%
2 99.00%
17 100.00%
2 99.00%
2 99.00%
3 99.90%
5 100.00%
2 99.00%
6 100.00%
5 100.00%
3 99.90%
2 99.00%
3 99.90%
2 99.00%
2 99.00%
5 100.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
6 100.00%
2 99.00%
3 100.00%
3 99.90%
2 99.00%
2 99.00%
3 99.90%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
4 100.00%
2 99.00%
3 99.90%
2 99.00%
3 99.90%
3 99.90%
2 99.00%
2 99.40%
2 99.00%
2 99.80%
3 99.90%
2 99.00%
3 99.90%
3 99.90%
3 99.90%
2 99.00%
2 99.30%
2 99.00%
2 99.00%
5 100.00%
4 99.90%
2 99.00%
4 100.00%
2 99.60%
2 99.00%
3 99.90%
3 100.00%
2 99.00%
3 99.90%
2 99.00%
3 99.90%
2 99.00%
2 99.70%
3 100.00%
2 99.00%
3 99.90%
2 99.00%
4 100.00%
2 99.00%
11 100.00%
3 99.90%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
5 100.00%
2 99.00%
2 99.00%
7 100.00%
2 99.00%
2 99.00%
2 99.00%
3 100.00%
6 100.00%
2 99.00%
2 99.00%
3 99.90%
4 100.00%
2 99.00%
2 99.00%
3 99.90%
2 99.00%
3 99.90%
4 100.00%
3 99.90%
4 100.00%
2 99.70%
2 99.00%
2 99.00%
2 99.00%
5 100.00%
2 99.90%
2 99.00%
7 100.00%
2 99.80%
2 99.00%
2 99.00%
3 99.90%
3 99.90%
2 99.00%
2 99.80%
2 99.00%
2 99.00%
2 99.00%
2 99.60%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
3 99.90%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
2 99.00%
3 99.90%
3 99.90%
4 100.00%
2 99.00%
3 100.00%
3 99.90%
2 99.00%
2 99.00%
2 99.00%
4 99.90%
2 99.00%
3 99.90%
3 99.90%
2 99.00%
2 99.00%
2 99.00%
3 99.90%
5 100.00%
2 99.00%
2 99.00%
2 99.00%
6 100.00%
2 99.00%
Supplementary Table 1. 32 validated transitions used in this study for quantitative analysis.
Retensio
Protin n time
Description Protein ID peptide sequence Precusor m/z Fragment m/z (min)
EGFR IPI00018274 YSFGATCVK 516.745 782.4 17.9
EGFR IPI00018274 YSFGATCVK 516.745 635.3 17.9
CBL IPI00027269 GTEPIVVDPFDPR 721.37 631.3 24.3
CBL IPI00027269 GTEPIVVDPFDPR 721.37 746.347 24.3
GRB2 IPI00021327 FNSLNELVDYHR 753.87 590.268 22.0
GRB2 IPI00021327 FNSLNELVDYHR 753.87 802.42 22.0
SOS1 IPI00020131 GLLVPALK 405.776 428.286 22.7
SOS1 IPI00020131 GLLVPALK 405.776 640.439 22.7
SHC1 IPI00021326 HLLLVDPEGVVR 673.893 656.372 22.8
SHC1 IPI00021326 HLLLVDPEGVVR 673.893 870.5 22.8
VAV2 IPI00004977 SHASGTYLIR 552.79 809.5 16.4
VAV2 IPI00004977 SHASGTYLIR 552.79 880.5 16.4
Ubiquitin IPI00179330 TITLEVEPSDTIENVK 894.47 1002.5 22.5
Ubiquitin IPI00179330 TITLEVEPSDTIENVK 894.47 1131.6 22.5
TPLLSSLpS1039ATSNNSTVACI
EGFR IPI00018274 DR 1144.04 1095.05 22.8
TPLLSSLpS1039ATSNNSTVACI
EGFR IPI00018274 DR 1144.04 634.3 22.8
TPLLSSLSApT1041SNNSTVACI
EGFR IPI00018274 DR 1144.04 1095.05 23.3
TPLLSSLSApT1041SNNSTVACI
EGFR IPI00018274 DR 1144.04 634.3 23.3
EGFR IPI00018274 MHLPSPTDSNFYR 822.85 773.86 20.3
EGFR IPI00018274 MHLPSPTDSNFYR 822.85 1165.5 20.3
Fragment
Collision Precurso Fragment
charge
Energy r charge series Fragment Nr
21 +2 y 7 +1
21 +2 y 6 +1
27.8 +2 y 5 +1
27.8 +2 y 6 +1
28.9 +2 y 4 +1
28.9 +2 y 6 +1
17.1 +2 y 4 +1
17.1 +2 y 6 +1
26.2 +2 y 6 +1
26.2 +2 y 8 +1
22.1 +2 y 7 +1
22.1 +2 y 8 +1
33.7 +2 y 11 +1
33.7 +2 y 10 +1
+2 y precursor-98 +2
38
+2 y +1
42 5
+2 y precursor-98 +2
38
+2 y +1
42 5
31 +2 y precursor-98 +2
31 +2 y y10-98 +1
Supplementary table 3. Information related to EGFR phospho-peptides. Peptide identification probabilities were more th
specified by the Peptide Prophet algorithm {Keller, 2002 #61}.
Fig. X! Tandem
List EGFR peptide sequnce Residue No. -log(e) Charge (+)
A (R)ELVEPLpTPSGEAPNQALLR(I) pT693 6.24 2
B (R)MHLPpSPTDSNFYR(A) (EGFR) pS991 3.89 2
C (R)MHLPpSPTDSNFFR(A) (Y998F) pS991 5.55 2
D (R)ALMDEEDMDDVVDADEYLIPQQGFFSpSPSTSR(T) pS1026 5.36 3
E (R)TPLLSpSLSATSNNSTVAcIDR(N) pS1037 6.11 2
F (R)TPLLSSLpSATSNNSTVAcIDR(N) pS1039 7.18 2
G (R)TPLLSSLSApTSNNSTVAcIDR(N) pT1041 7.48 2
H (R)TPLLSSLSATpSNNSTVAcIDR(N) pS1042 9.74 2
I (R)pYSSDPTGALTEDSIDDTFLPVPEYINQSVPK(R) pY1069 7.59 3
J (R)YSSDPTGALTEDpSIDDTFLPVPEYINQSVPK(R) pS1081 5.00 3
K (R)YSSDPTGALTEDSIDDpTFLPVPEYINQSVPK(R) pT1085 6.55 3
L (R)YSSDPTGALTEDSIDDTFLPVPEpYINQSVPK(R) pY1092 10.33 3
M (R)YSSDPTGALTEDSIDDTFLPVPEYINQpSVPK(R) pS1096 6.43 3
N (K)RPAGpSVQNPVYHNQPLNPAPSR(D) pS1104 4.82 3
O (K)RPAGSVQNPVpYHNQPLNPAPSR(D) pY1110 7.00 3
P (R)DPHYQDPHSTAVGNPEpYLNTVQPTcVNSTFDSPAHWAQK(G)pY1138 6.96 4
Q (K)GSHQIpSLDNPDYQQDFFPK(E) pS1166 6.57 2
R (K)GSHQISLDNPDpYQQDFFPK(E) pY1172 8.66 2
S (K)GSHQIpSLDNPDpYQQDFFPK(E) pS1166,pY1172 3.96 3
T (K)GSTAENAEpYLR(V) pY1197 7.02 2
probabilities were more than 95% as
}.
Observed
m/z Delta PPM
1057.53 -0.02
822.85 3.17
814.85 0.20
1229.86 5.33
1144.04 2.44
1144.04 6.39
1144.04 1.59
1144.04 3.29
1160.20 -3.16
1160.20 3.69
1160.20 3.37
1160.20 3.79
1160.21 6.32
827.07 1.16
827.07 0.72
1123.00 1.48
1158.50 1.78
1158.50 0.09
799.33 0.69
645.77 -0.22
SRM intensities normalized to internal EGFR peptide, and associated statistical calculations
Normalized SRM intensites (x0.001)
Rep 1 Rep 2 Rep 3 Mean CV SE Rep 1 Rep 2 Rep 3 Mean CV SE TTEST *p=0.05 **p=0.01
WT-EGF WT+EGF
CBL 21.32 4.24 8.28 11.28 0.646 4.2067 CBL 85.61 51.33 51.79 62.91 0.26 9.3 5.07 ***
Fold 1.00 1.00 1.00 1.00 4.02 12.11 6.25 7.46 0.46 2.0 3.28
GRB2 16.52 28.05 28.97 24.51 0.2311 3.2706 GRB2 59.47 105.00 61.43 75.30 0.28 12.1 4.04 **
Fold 1.00 1.00 1.00 1.00 3.60 3.74 2.12 3.15 0.23 0.4 5.09 Data Analysis and Statistical
SHC1 22.77 43.31 34.83 33.64 0.2506 4.8671 SHC1 48.86 100.33 #### 97.35 0.39 22.2 2.8 * Calculations:
Fold 1.00 1.00 1.00 1.00 2.15 2.32 4.10 2.85 0.31 0.5 3.63 1. Normalize SRM intensities by
Vav2 22.54 5.81 5.79 11.38 0.6932 4.5564 Vav2 24.81 7.77 10.14 14.24 0.53 4.4 0.45 using intrinsic EGFR peptide.
Fold 1.00 1.00 1.00 1.00 1.10 1.34 1.75 1.40 0.19 0.2 2.55 2. Calculate EGF-induced change
Ubiq 1.64 1.12 1.34 1.37 0.1554 0.1227 Ubiq 7.92 2.82 3.35 4.70 0.49 1.3 2.51 * in SRM intensity; express as fold
Fold 1.00 1.00 1.00 1.00 4.83 2.52 2.50 3.28 0.33 0.6 3.62 relative to control unstimulated.
SOS1 57.14 29.04 13.31 33.16 0.5467 10.468 SOS1 146.02 36.00 25.89 69.31 0.78 31.4 1.09 3. Calculate the mean difference
Fold 1.00 1.00 1.00 1.00 2.56 1.24 1.95 1.91 0.28 0.3 2.94 (n=3), CV, SE.
Y998F-EGF Y998F+EGF 4. Apply one-tailed (since
CBL 8.53 10.57 9.41 9.50 0.0883 0.4844 CBL 63.68 25.26 26.15 38.36 0.47 10.3 2.79 * reasonable expectation that EGF
Fold 1.00 1.00 1.00 1.00 7.47 2.39 2.78 4.21 0.55 1.3 2.41 causes increased association)
GRB2 20.78 13.88 13.39 16.02 0.2108 1.949 GRB2 50.24 70.15 24.47 48.28 0.39 10.8 2.94 * Student's t-test):
Fold 1.00 1.00 1.00 1.00 2.42 5.06 1.83 3.10 0.45 0.8 2.59 t = ∆Means/√*SE 2+SE2 (4df)
SHC1 19.72 30.05 99.15 49.64 0.7104 20.36 SHC1 30.19 88.73 #### 76.53 0.44 19.6 0.95
t crit: *p=0.05 2.132
Fold 1.00 1.00 1.00 1.00 1.53 2.95 1.12 1.87 0.42 0.5 1.91
**p=0.01 3.747
Vav2 10.85 4.07 3.22 6.05 0.5649 1.9721 Vav2 20.52 4.80 4.66 9.99 0.74 4.3 0.83
***p=0.005 4.604
Fold 1.00 1.00 1.00 1.00 1.89 1.18 1.45 1.51 0.19 0.2 2.99
Ubiq 1.24 0.81 0.48 0.84 0.3719 0.1807 Ubiq 4.17 2.31 2.46 2.98 0.28 0.5 4.13 **
Fold 1.00 1.00 1.00 1.00 3.36 2.86 5.18 3.80 0.26 0.6 4.87
SOS1 64.12 24.88 10.17 33.06 0.6889 13.147 SOS1 93.63 31.11 22.29 49.01 0.65 18.3 0.71
Fold 1.00 1.00 1.00 1.00 1.46 1.25 2.19 1.63 0.25 0.2 2.72
S991A-EGF S991A+EGF
CBL 19.92 10.26 4.21 11.46 0.5645 3.7356 CBL 50.72 45.84 31.78 42.78 0.19 4.6 5.26 ***
Fold 1.00 1.00 1.00 1.00 2.55 4.47 7.55 4.86 0.42 1.2 3.24
GRB2 22.36 14.13 19.45 18.65 0.1828 1.9674 GRB2 41.37 60.18 55.56 52.37 0.15 4.6 6.71 ***
Fold 1.00 1.00 1.00 1.00 1.85 4.26 2.86 2.99 0.33 0.6 3.49
SHC1 24.39 22.04 55.71 34.05 0.4508 8.8616 SHC1 24.46 88.35 89.56 67.46 0.45 17.6 1.7
Fold 1.00 1.00 1.00 1.00 1.00 4.01 1.61 2.21 0.59 0.7 1.61
Vav2 19.84 3.57 4.57 9.33 0.7982 4.2978 Vav2 20.14 3.48 9.76 11.13 0.62 4.0 0.31
Fold 1.00 1.00 1.00 1.00 1.02 0.98 2.14 1.38 0.39 0.3 1.21
Ubiq 1.87 1.00 0.77 1.21 0.3931 0.2752 Ubiq 6.62 1.32 2.01 3.32 0.71 1.4 1.52
Fold 1.00 1.00 1.00 1.00 3.53 1.32 2.62 2.49 0.36 0.5 2.85
SOS1 101.63 9.54 21.04 44.07 0.9297 23.654 SOS1 105.40 23.05 23.91 50.78 0.76 22.3 0.21
Fold 1.00 1.00 1.00 1.00 1.04 2.42 1.14 1.53 0.41 0.4 1.46
10
WT Y998F S991A
CBL 7 4 5
9
GRB2 3 3 3
SHC1 3 2 2
8
VAV2 1 2 1
EGF-Stimulated Association (Fold)
UBB 3 4 2 7
SOS1 2 2 2
6 WT
WT SE Y998F SE S991A SE
CBL 1.97 1.33 1.19 5
GRB2 0.42 0.81 0.57 Y998
SHC1 0.51 0.45 0.75 4
VAV2 0.16 0.17 0.31
F
UBB 0.63 0.57 0.52 3
SOS1 0.31 0.23 0.36
2
1
0
CBL GRB2 SHC1 VAV2 UBB SOS1
Associated Protein
Comparing Absolute Level of Activation
WT v. Y998F t calc TTEST t calc
Rep 1 Rep 2 Rep 3 Mean CV SE Mean∆/SE p=0.01 p ∆Mean/√[SE2+SE2]
CBL WT 85.6061 51.3333 51.7857 62.908 9.27
Y998F 63.6792 25.261 26.145 38.362 10.34
∆ 21.9268 26.0724 25.6407 24.547 0.0758 1.07 22.85 ** 0.222 1.7681
GRB2 WT 59.4697 105 61.4286 75.299 12.13
Y998F 50.2358 70.1461 24.4656 48.283 10.80
∆ 9.23385 34.8539 36.9629 27.017 0.4665 7.28 3.71 0.246 1.6634
SHC1 WT 48.8636 100.333 142.857 97.351 22.19
Y998F 30.1887 88.7265 110.687 76.534 19.62
∆ 18.675 11.6068 32.1701 20.817 0.4098 4.93 4.23 0.597 0.7029
Data Analysis and Statistical
VAV2 WT 24.8106 7.76667 10.1429 14.24 4.35 Calculations:
Y998F 20.5189 4.80167 4.65649 9.9923 4.30 1. Normalize SRM intensities by using
∆ 4.29174 2.965 5.48637 4.2477 0.2424 0.59 7.14 0.601 0.6945 intrinsic EGFR peptide.
2. Calculate EGF-induced change in
UBB WT 7.91667 2.82 3.35 4.6956 1.32 SRM intensity.
Y998F 4.16981 2.31315 2.46183 2.9816 0.49 3. Determine difference between WT
∆ 3.74686 0.50685 0.88817 1.714 0.8436 0.83 2.05 0.376 1.2176 and variants for n=3 independent
experiments (and CV, SE).
SOS1 WT 146.023 36 25.8929 69.305 31.41 4. Apply Student's t-test. Two-tailed
Y998F 93.6321 31.1065 22.2901 49.01 18.34 (since cannot predict if protein
∆ 52.3907 4.89353 3.60278 20.296 1.1185 13.11 1.55 0.672 0.558 association should be more or less in
WT v. S991A variants compared to WT):
CBL WT 85.6061 51.3333 51.7857 62.908 9.27 t = Mean ∆/SE (2df)
S991A 50.7194 45.8387 31.7845 42.781 4.63 t crit: **p=0.01 9.925
∆ 34.8866 5.49466 20.0012 20.128 0.5962 6.93 2.91 0.188 1.9426
GRB2 WT 59.4697 105 61.4286 75.299 12.13
S991A 41.3669 60.1793 55.5556 52.367 4.62
∆ 18.1028 44.8207 5.87302 22.932 0.7092 9.39 2.44 0.223 1.7662
SHC1 WT 48.8636 100.333 142.857 97.351 22.19
S991A 24.4604 88.3483 89.5623 67.457 17.56
∆ 24.4032 11.9851 53.2949 29.894 0.5789 9.99 2.99 0.437 1.0566
VAV2 WT 24.8106 7.76667 10.1429 14.24 4.35
S991A 20.1439 3.48271 9.76431 11.13 3.97
∆ 4.66672 4.28395 0.37855 3.1097 0.6231 1.12 2.78 0.689 0.5281
UBB WT 7.91667 2.82 3.35 4.6956 1.32
S991A 6.61871 1.31882 2.01347 3.317 1.36
∆ 1.29796 1.50118 1.33653 1.3786 0.0639 0.05 27.10 ** 0.584 0.7277
SOS1 WT 146.023 36 25.8929 69.305 31.41
S991A 105.396 23.0474 23.9057 50.783 22.30
∆ 40.627 12.9526 1.98713 18.522 0.8778 9.39 1.97 0.715 0.4809