; Title Differential identification of Mycobacterium tuberculosis
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Title Differential identification of Mycobacterium tuberculosis

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									Title: Differential   identification   of   Mycobacterium   tuberculosis   complex   and
nontuberculous mycobacteria by duplex PCR assay using the RNA polymerase gene
(rpoB)


Abstract: A novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs
of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM),
respectively, with two different sets of primers was used to differentially identify 44
reference strains and 379 clinical isolates of mycobacteria in a single-step assay.
Showing 100% sensitivity and specificity, the duplex PCR method could clearly
differentiate M. tuberculosis complex and NTM strains. In addition, restriction fragment
length polymorphism analysis and direct sequencing of the amplicon of NTM could be
used to supplement species identification.

								
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