Lunch and Learn
TRANSGENIC 101 RODENT HUSBANDRY
Presented by: Jerome Donohoe RLAT
2007 National AALAS Annual Meeting in Charlotte NC
The Set Up
Bottom / top / wire lid / filtered top
Static / ventilated
Bedding / food / water
Nesting material / structure / nothing added
Investigate all products for supportive factors impacting research
Cage bottoms to top
Size – density - height
Plastic cages can release compounds disrupting research (polycarb)
Bottoms, bottles, structures ……
Static or Ventilated caging
Protection, intra-cage environment ……
Bedding type can harbor hidden unwanted bio-burdens
Corn-cob, some paper products ……
Feed type appropriate for the model being used
High fat, low salt, purified, sterile ……
Feed delivery method
Pellet, powdered, liquid ……
Water type appropriate for use
Acidified, hypo-clorinated, tap ……
Water delivery method
Automated, bottle, pouch, gel ……
Nesting material for breeders / enrichment
Material, litter / hiding / climbing ……
The purpose is to provide support to the animal and research mission with clean, dry and
sanitary conditions appropriate for the science at hand …..
Housing densities and cage change frequencies
Increase production, minimize cannibalization, and maintain phenotype
Single housed animals can go out to two weeks between changes
Males, females, stud ……
Multiple housed animals might make it
2 to 5 per cage, age, genetic pre-disposition ……
Breeders pose the challenge
Gestation, age of litter(s), post partum ……
Weanlings will need lots of caging and space on time!!!
Reductions can be accomplished by typing and managing colonies.
Peak production / mice
80 lines x 10 breeders per line = 800 breeder cages
Average litter size (6) x 250 litters / week = 1500 mice
1500 mice weaned = possibility of minimum 300 extra cages / week
Peak production / rats
59 lines x 12 breeders per line = 720 breeder cages
Average litter size (10) x 240 litters / week = 2400 rats
Age Breeder pair 1
3 weeks of age
8 weeks pregnant
11 wean 5m / 5f to research
12 litter two born
14 Age Breeder pair 2
15 weaned set up breeder 2 3 weeks
16 litter 3 born 4
19 litter 3 weaned to research 7
20 litter 4 born 8 weeks pregnant
23 litter 4 weaned to research 11 wean 5m / 5f to research
24 litter 5 born 12 litter two born
27 litter 5 weaned to research 15 weaned set up pair 3
28 litter 6 born 16 litter 3 born
31 litter 6 weaned to research retire? 19 litter 3 weaned to research
20 litter 4 born
23 litter 4 weaned to research
24 litter 5 born
27 litter 5 weaned to research
28 litter 6 born
31 litter 6 weaned to research?
Job Opportunities and Qualifiers
A private contractor with a Government Agency is looking for a Breeding Specialist to perform
Transgenic technical duties as a member of a contract team providing animal husbandry
support in an AALAC accredited facility at Research Triangle Park, Durham NC.
Responsibilities include, but are not limited to:
· Provide oversight of Transgenic, Isolator, and Production activities
· Train, coordinate and oversee technicians providing technical support.
· Prepare protocols and coordinate with investigators and facility directors to ensure the
proper maintenance and efficiency of the colonies.
· Provide weekly and monthly reports.
· Comply with all NIEHS, SOPs, USDA Animal Welfare Act Regulations, and 'The Guide for the
Care and Use of Laboratory Animal.'
· Assist in creating and maintaining local SOPs, spreadsheets, databases, and computer
software in support of position requirements and to facilitate accurate record keeping.
· Attend and participate in periodic and ad hoc meetings as directed by the Manager.
· Participate in Supervisor weekend/holiday on call rotation schedule.
Qualified candidates should apply to Maureen Trogdon by sending resumes to
Candidates may also contact Maureen by telephone (919) 541-0557 or Fax (919) 316.4572.
LATG Certification or ability to obtain certification within one year of hire.
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