APM003_Gilston Peristaltic Pump

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					                               STANDARD OPERATING PROCEDURE

     SP # APM003             Version: 1.0            Replaces: N/A           Written by:    Maryline Chee Kimling
                             Date:      07/07/10                             Reviewed by: Dana Morgan
     Page 1 of 6             Revise by: 07/07/13                             Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne


                                   USE OF PERISTALTIC PUMP

1.         Introduction

           This SOP discusses the procedure and safety guidelines for the use of Gilson Minipuls 3
           Peristaltic Pump.

           The peristaltic pump can be used to deliver liquids at a controlled flow rate if required. A
           liquid can be transferred to another vessel by allowing the liquid to pass through a tube
           which is in contact with a set of rollers (at the pump head) whose speed can be adjusted.
           The compression of the tube against the moving rollers enables the liquid to move
           through the tube.

           There are four channels available at the pump head. This means that four different
           connections can be made at a time. Internal diameter tubing size can range between
           0.2 mm to 4 mm. The speed control ranges between 1 and 48 rpm.

2.         Scope

           This procedure applies to all staff, students and visitors of the Advanced Porous Materials
           Group in the School of Chemistry at the University of Melbourne that work in the
           laboratory and have the potential to use the Gilson Peristaltic Pump.

3.         Safety

           General safety precautions:
            Electric shock hazards can occur in the event of faulty components.
            Obtain and consult MSDS accordingly when handling chemicals. Spills must be
              contained, if possible by consulting relevant MSDS. Else, help must be sourced from
              trained personnel.
            Replace tubing when it shows signs of wear such as flatness or cracking. (Damaged
              tubes can cause erratic flow as well as increased risks of tube rupture and spills).
            The user must check compatibility of tube with the liquid that he or she is using prior
              to use and appropriate tubings need to be used.
            Only four (4) types of tubing can be fitted on this model and are listed as follows:
                   PVC (tygon);
                   Silicone;
                   Polypropylene; and
                   Isoversinic (Viton).
            Please refer to the following website as a guide:
              www.coleparmer.com/techinfo/chemcomp.asp

4.         Licences and Permits
                               STANDARD OPERATING PROCEDURE

     SP # APM003             Version: 1.0            Replaces: N/A           Written by:    Maryline Chee Kimling
                             Date:      07/07/10                             Reviewed by: Dana Morgan
     Page 2 of 6             Revise by: 07/07/13                             Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne

           N/A

5.         Training and Competency

           New users are required to seek training and approval from an approved trainer (the
           instrument supervisor or any trained member of the Advanced Porous Materials Group)
           prior to the use of the instrument. Competency of the trainee to use the instrument will be
           assessed by close observation by an approved trainer.

6.         Risk Assessments

           Refer to the following risk assessment forms:
            Use of Gilson Minipuls 3 peristaltic pump, assessed 07/07/10.
            Use of electrically operated equipment, assessed 20/06/07.

7.         Equipment and Maintenance / Handling and Storage / Labelling

           The peristaltic pump and accessories are located in Room 404.

           Ensure that the pump is covered with the plastic cover after use to prevent dust particles
           from damaging the pump head.

           Replace tubing when it shows signs of wear such as flatness or cracking. (Damaged tubes
           can cause erratic flow as well as increased risks of tube rupture and spills).

           There is a small Isoversinic drain tube inside the pump, with an outlet at the base of the
           pump. In the event that the tubing at the pump head is ruptured, this will prevent liquid
           from entering the instrument. However in the event of a spill, ensure that the equipment
           is cleaned thoroughly.

           For equipment maintenance:
                  John Morris Scientific Pty. Ltd.
                  C/O Victorian Territory Manager
                  1st Floor, 91 Whitehorse Rd,
                  Balwyn VIC 3103
                  www.johnmorris.com.au
                  Tel: +61 3 9816 6700 or +61 1800 552 724

8.         Operating Procedures

           1. Install tube at the pump head (see Figure 1).
               Only tubings with retaining stops can be used to fit the pump head (the colour of
                  the retaining stops indicates the internal diameter size of the tubing).
               Unlock each channel, if required, by pressing the trigger key (forward RHS arm)
                  towards the rollers.
                               STANDARD OPERATING PROCEDURE

  SP # APM003               Version: 1.0             Replaces: N/A           Written by:    Maryline Chee Kimling
                            Date:      07/07/10                              Reviewed by: Dana Morgan
  Page 3 of 6               Revise by: 07/07/13                              Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne

               Position tubing around the set of rollers. The tube is kept in place with the
                retaining stops.
               Swing compression cam (LHS arm) onto the tubing and close by pressing the
                locking key (back RHS arm)
               The adjustment screw can be used to tighten or loosen tubing against the set of
                rollers as accordingly. NOTE: Degree of tightening influences the flow rate. The
                higher the degree of tightening, the shorter the lifespan of the tube will be.




                                       Figure 1. Pump head schematics

        2. The length of the tube can be adjusted by connecting with the extension tubing
            (without retaining stops). Appropriate connectors should be used according to the
            type of tubings used.
        3. Switch pump on at the power point and then at the rear of the instrument. (A reading
            is displayed when instrument is switched on).
        4. Select the desired speed by pressing (+) to increase the speed or (–) to decrease the
            speed (in rpm, maximum value is 48 rpm).
        5. To start the pump, press         for a clockwise flow (from left to right) or  for an
            anticlockwise flow (from right to left). A (+) sign will indicate a clockwise flow
            while a (–) indicates an anticlockwise flow.
        6. Press the RABBIT key to purge or to prime tubing, which sets to the maximum speed
            of 48 rpm.
        7. Press STOP key to halt the flow/pump.
        8. When experiment is completed, switch off the pump at the rear of the instrument and
            at the power point.
        9. Remove tubing.
             First unscrew the adjustment screw.
             Push the trigger arm towards the rollers (front RHS arm).
             And swing compression cam back (LHS arm).
        10. Wash tubing with copious water. The tubing can be air-dried or dried in an oven at ~
            40–50 ºC.

        NOTE: the smaller the tube diameter, the smaller the pulsations, the faster the head has to
        rotate, shortening the lifespan of the tube.
                               STANDARD OPERATING PROCEDURE

     SP # APM003             Version: 1.0            Replaces: N/A           Written by:    Maryline Chee Kimling
                             Date:      07/07/10                             Reviewed by: Dana Morgan
     Page 4 of 6             Revise by: 07/07/13                             Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne


9.         Controls and Calibrations

           N/A

10.        Waste Disposal

           Liquid Waste Disposal: Dispose of solvents in the appropriate waste container (e.g. water
           soluble waste solvent, water insoluble waste solvent, halogenated waste solvent).

           Solid Waste Disposal: Rinsed tubing and connectors may be disposed of in a waste
           receptacle for collection and disposal in an authorised landfill.

11.        Relevant Documents / References

              Minipuls 3 Peristaltic Pump User’s Guide (LT801121/N).

           For tubing/chemical compatibilities, please check:
            www.coleparmer.com/techinfo/chemcomp.asp

           Risk assessments applicable to the SOP are listed in Section 6.

12.        Signage / Summaries / Templates

           Competency Training Records Form – Attached, see page 5.
            Copies of this form are to be stored and filed and in the Advanced Porous Materials
             Competency Records Folder.

13.        Appended Material

           N/A
                                 STANDARD OPERATING PROCEDURE

  SP # APM003               Version: 1.0             Replaces: N/A           Written by:    Maryline Chee Kimling
                            Date:      07/07/10                              Reviewed by: Dana Morgan
  Page 5 of 6               Revise by: 07/07/13                              Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne

                       ADVANCED POROUS MATERIALS LABORATORY

                 COMPETENCY TRAINING RECORDS –PERISTALTIC PUMP


                                                                                                Competency
Date                Name                 University/Dept.               Trainer
                                                                                             Date    Trainee   Trainer
                                 STANDARD OPERATING PROCEDURE

  SP # APM003               Version: 1.0             Replaces: N/A           Written by:    Maryline Chee Kimling
                            Date:      07/07/10                              Reviewed by: Dana Morgan
  Page 6 of 6               Revise by: 07/07/13                              Authorised by: Rachel A. Caruso
Advanced Porous Materials Laboratory, School of Chemistry, The University of Melbourne

				
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