Chapter 5 Study Questions by IB9A1L8


									                       Chapter 5 Study Questions
These will be due on Thursday before your exam. Use them to help study for the test.

 1. Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate
    roughly the comparative electrophoretic mobility’s of DNAs with 150, 600, and
    1200 bp (25pts).
 2. Compare and contrast SDS-PAGE and two-dimensional gel electrophoresis of
    proteins (50pts).
 3. What is SDS? What are its functions in SDS-PAGE (10pts)?
 4. Compare and contrast the two types of ion exchange chromatography (30pts).
 5. Describe gel filtration chromatography (50pts).
 6. Describe the principles involved in DNA fingerprinting (50pts).

To top