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Chapter 5 Study Questions These will be due on Thursday before your exam. Use them to help study for the test. 1. Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the comparative electrophoretic mobility’s of DNAs with 150, 600, and 1200 bp (25pts). 2. Compare and contrast SDS-PAGE and two-dimensional gel electrophoresis of proteins (50pts). 3. What is SDS? What are its functions in SDS-PAGE (10pts)? 4. Compare and contrast the two types of ion exchange chromatography (30pts). 5. Describe gel filtration chromatography (50pts). 6. Describe the principles involved in DNA fingerprinting (50pts).
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