Chapter 5 Study Questions by IB9A1L8

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									                       Chapter 5 Study Questions
These will be due on Thursday before your exam. Use them to help study for the test.

 1. Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate
    roughly the comparative electrophoretic mobility’s of DNAs with 150, 600, and
    1200 bp (25pts).
 2. Compare and contrast SDS-PAGE and two-dimensional gel electrophoresis of
    proteins (50pts).
 3. What is SDS? What are its functions in SDS-PAGE (10pts)?
 4. Compare and contrast the two types of ion exchange chromatography (30pts).
 5. Describe gel filtration chromatography (50pts).
 6. Describe the principles involved in DNA fingerprinting (50pts).

								
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