Initial Dilution
Substance nanomoles micromoles desired concentration (uM) Volume to add (L)
U1C 31.9 0.0319 200 0.00015950
M13 17.9 0.0179 200 0.00008950
Ultramer 20 0.02 20 0.00100000
[0]0 35 0.035 200 0.00017500
[0]21 28.8 0.0288 200 0.00014400
[0]42 33.5 0.0335 200 0.00016750
[0]63 37.3 0.0373 200 0.00018650
[0]84 32.7 0.0327 200 0.00016350
[0]105 30.2 0.0302 200 0.00015100
[0]126 31.8 0.0318 200 0.00015900
[0]147 33.4 0.0334 200 0.00016700
[0]168 32.5 0.0325 200 0.00016250
Nanodrop Measurements
Volume to add (uL) (ng/mL) 1 (ng/mL) 2
159.5 260.8 259.1
89.5 3167.2 3117.9
1000 1228.7 1214.1
175 2322.6 2334.4
144 2227.3 2208.6
167.5 2195.7 2220
186.5 2238.9 2224.7
163.5 2248.2 2212.6
151 2256.2 2274.3
159 2258.8 2353.9
167 2087 1904.7
162.5 2352.9 2347.8
Avg. (ng/mL) MW (g/moles) uL removed concentration (uM)
259.95 4626.1 3 56.19204081
3142.55 17122.3 13.5 183.5355063
1221.4 58204.8 3 20.98452361
2328.5 12825.4 3 181.5537917
2217.95 12909.4 3 171.8089144
2207.85 12864.3 3 171.6261281
2231.8 12826.3 3 174.0018556
2230.4 12779.3 3 174.5322514
2265.25 12906.4 3 175.5136986
2306.35 12786.3 3 180.3766531
1995.85 12808.3 3 155.8247386
2350.35 12884.4 3 182.4182733
Second Dilution
current umoles total volume (uL) for 100 uM volume to add (uL) Substance
0.008794054 no second dilution done - additional 3 uL removed U1C
0.013948698 139.4869848 63.48698481 M13
0.02092157 2092.157004 1095.157004 Ultramer
0.031227252 312.2725217 140.2725217 [0]0
0.024225057 242.2505694 101.2505694 [0]21
0.028232498 282.3249808 117.8249808 [0]42
0.03192934 319.293405 135.793405 [0]63
0.028012426 280.1242635 119.6242635 [0]84
0.025976027 259.760274 111.760274 [0]105
0.028138758 281.3875789 125.3875789 [0]126
0.025555257 255.5525714 91.55257138 [0]147
0.029095715 290.9571459 131.4571459 [0]168
2nd Nanodrop For nanoparticle chain ladder (100 uL reaction volu
(ng/mL) concentration (uM) uL removed Volume needed (L)
1765 103.0819458 1.5
625.7 10.74997251 1.5 9.30235E-07
1311 102.2190341 1.5 9.78291E-07
1328.4 102.9017615 1.5 9.71801E-07
1343.2 104.4129879 1.5 9.57735E-07
1329.4 103.646414 1.5 9.64819E-07
1308.5 102.3921498 1.5 9.76637E-07
1311.6 101.6240005 1.5 9.8402E-07
1298.8 101.5774696 1.5 9.8447E-07
1388.6 108.4140752 1.5 9.22389E-07
1330.7 103.2799354 1.5 9.68242E-07
Reaction
1
2
3
4
5
6
7
8
9
10
oparticle chain ladder (100 uL reaction volume, 100 nM ultramer, 1 uM staples)
Volume needed (uL) Substance
U1C
M13
0.930234937 Ultramer
0.978291381 [0]0
0.971800662 [0]21
0.957735259 [0]42
0.964818715 [0]63
0.976637371 [0]84
0.984019518 [0]105
0.98447028 [0]126
0.922389457 [0]147
0.968242279 [0]168
Contents Volume Solution Needed (uL) Volume Buffer (uL)
Ultramer 99.06976506 50
Ultramer, 0 98.09147368 50
Ultramer, 0, 21 97.11967302 50
Ultramer, 0, 21, 42 96.16193776 50
Ultramer, 0, 21, 42, 63 95.19711905 50
Ultramer, 0, 21, 42, 63, 84 94.22048167 50
Ultramer, 0, 21, 42, 63, 84, 105 93.23646216 50
Ultramer, 0, 21, 42, 63, 84, 105, 126 92.25199188 50
Ultramer, 0, 21, 42, 63, 84, 105, 126, 147 91.32960242 50
Ultramer, 0, 21, 42, 63, 84, 105, 126, 147, 168 90.36136014 50
Volume Water (uL)
49.0697651
48.0914737
47.119673
46.1619378
45.197119
44.2204817
43.2364622
42.2519919
41.3296024
40.3613601