Biology Homework – Week 8
Review of last week’s assignments:
1. “Environmental Challenges to Biodiversity” Power point project is due Friday, October 28. Rubric is on the weblog.
2. Read text pp. 217-219 to review DNA replication. Be sure to analyze the pictures on the pages too. Write the
questions and answers for “Check your Progress” on p. 219. Submit your Q&A Monday by 10:45 in Rm. 134
3. Read about PCR and perform a virtual lab using PCR - http://learn.genetics.utah.edu/content/labs/pcr/ - Complete the
response sheet (see below). Be sure to read the additional article and to view the additional animation – Completed
response sheet is due in class Monday.
4. Study and Review – DNA Quiz on Wednesday, 10/26, covering Ch.3 (sec. 3.5), Ch. 12 (section 12.1 to 12.2), PCR
virtual lab, DNA Isolation & Electrophoresis) and supplemental (video and websites).
Monday 1. Review this lab animation in review/preparation for gel electrophoresis procedure in
10/24/11 class:
http://www.sumanasinc.com/webcontent/anisamples/majorsbiology/gelelectrophoresis.
html
2. Study and Review – DNA Quiz on Wednesday Thursday covering Ch.3, Ch. 12
(section 12.1 to 12.2), 9 (section 9.1, 9.3,9.4), labs (PCR virtual lab, DNA Isolation &
Electrophoresis) and supplemental (video and websites).
Tuesday 3. Complete Lab questions corresponding to the DNA isolation techniques performed in
10/25/11 class.
4. Study and Review – DNA Quiz on Thursday covering Ch.3, Ch. 12 (section 12.1 to
12.2), 9 (section 9.1, 9.3,9.4), labs (PCR virtual lab, DNA Isolation & Electrophoresis)
and supplemental (video and websites).
Wednesday 1. Study and Review.
10/26/11 2. Complete laboratory questions regarding procedure. Analysis of gel results will be
tomorrow. The following site will help you answer the lab follow-up questions:
Gel Electrophoresis of DNA (University of Illinois):
http://www.life.uiuc.edu/molbio/geldigest/electro.html
Principles of Gel Electrophoresis:
http://www.vivo.colostate.edu/hbooks/genetics/biotech/gels/principles.html
Agarose Gel Electrophoresis of
DNA:http://www.vivo.colostate.edu/hbooks/genetics/biotech/gels/agardna.html
Thursday 1. Study and Review.
10/27/11
Friday 1. Study and Review
10/28/11 2. Compete the Stem Cell Information Search Packet (see below)
3. Review lecture: http://learn.genetics.utah.edu/content/tech/stemcells/scintro/
4. Read this on-line article: “What are the risks of cloning?”
http://learn.genetics.utah.edu/content/tech/cloning/cloningrisks/index.html
Name:____________________________________________________________
**An interactive tutorial on stem cells. http://www.umich.edu/stemcell/tutorial/
Additional resource: “The Nature of Stem Cells”
http://learn.genetics.utah.edu/content/tech/stemcells/scintro/
**As you view the interactive tutorial on stem cells answer the following questions:
I. Stem Cells Defined:
What is a stem cell?
a.
b.
Identify three types of stem cells:
Type of Stem Cell Where does this What is this Notes:
stem cell come stem cell
from? capable of
doing?
Embryonic Stem
Cell
Umbilical Cord
Stem Cell
Adult Stem Cell
II. Embryonic Stem Cells
_______________ and _______________ undergo fertilization to become a ZYGOTE
ZYGOTE undergoes cell division (aka MITOSIS) for 3-5 days and forms a ___________________
Before CELL DIFFERENTIATION occurs the individual cells of the blastocyst must be separated
from each other.
What is a KEY Difference between Embryonic Stem Cells and Adult Stem Cells?
III. Cell Specialization:
What does it mean for a cell to become “differentiated”?
How many chromosomes are in every cell of a human? (count them)____________
What has to occur for cells to become specialized?
Is specialization a permanent or temporary process? Explain.
IV. Adult Stem Cells:
Why are adult stem cells less versatile than embryonic stem cells?
Identify two examples of adult stem cells and state what they will differentiate into.
V. Nuclear Transfer
What is the benefit of a person using his or her own adult stem cells for medical treatments?
What is therapeutic cloning?
What are the benefits of therapeutic cloning?
Explain the steps involved in nuclear transplantation.
State/Explain a MAJOR BIOETHICAL concern with therapeutic cloning.
VI. Drug Testing:
How can stem cell research be incorporated into drug research?
Provide two examples of stem cell use for drug research.
a._______________________________________________
b._______________________________________________
VII. Place the following terms in order from MOST VERSATILE to LEAST VERSATILE.
Define and provide a cell-related example for each:
Multipotent Totipotent Differentiated Pluripotent
___________________ _________________ __________________ ___________________
most versatile cell Least versatile cell
COSI Experts Series – Thought Challenge
Stem Cells and Regenerative Medicine
(By Michael Gilkey and Debra Grega)
What Would Dr. Zoidberg Do?
To prepare for the stem cell challenge go to at least two of the following websites so you can help
Dr. Zoidberg fix his fellow Futurama friends.
http://stemcells.nih.gov/info/basics/basics4.asp
From the NIH webpage, this gives a good overview of adult stem cells.
http://www.pbs.org/wgbh/nova/sciencenow/3209/04.html
A NOVA segment on stem cells that includes video, text and animations about stem cells.
http://lifesciences.umich.edu/research/featured/tutorial.html
An interactive tutorial on stem cells.
Hopefully you now know as much as Dr. Zoidberg about stem cells, so now let’s go to your first
patient….
#1 Your first patient comes to you with a very
important problem; he had to wrestle Robot Paul
Bunyan and now has an amputation problem.
Yep, you guessed it, we are going to need another
leg. It is a good thing it is the year 3000 because
all you have to do to assist Dr. Zoidberg is come
up with the necessary stem cells to grow a new
leg. From what you have read, what specific types
of stem cells do you think Dr. Zoidberg could use
and why would you use them?
Dr. Zoidberg wants you to remember that
there are a lot of different tissues in the
leg including bone, muscle and nerve so
make sure you have all the necessary
kinds of stem cells present.
#2 There’s been another tragic accident and Philip
J. Fry has been pierced through the chest by a
huge bee. Fortunately for him, the stinger missed
his heart and swerved around most of his
intestines. However, he has damage to his spinal
cord. Without your help he will be paralyzed from
the waist down and thereby confined to a hover
wheelchair for the rest of his life. We are going to
need to repair his spinal cord. What types of stem
cells would you like to use and how do you think
they will repair the injury?
Dr. Zoidberg remembered something
from some old history books about there
being two main kinds of stem cells; adult
and embryonic. Make sure to consider
both when treating the patients.
#3 You have your last patient for the day; Professor Hubert J. Farnsworth is really, really, really old
and has been having trouble drinking his morning coffee due to excessive shaking. It turns out he has
been diagnosed with Parkinson’s disease and lacks the necessary dopamine producing neurons to keep
him functioning normally. What types of stem cells do you think could be injected so that he could
recover and resume his passion for java?