Introduction to the Microscope _Virtual Lab_

							       Introduction to the Microscope (Virtual Lab)                                             Name __________________________
Part Instructions: you will be looking at virtual microscopes and pictures to complete the questions below.
A
       1. Go to the E-Lab page and examine the microscope pictured. http://www.biologycorner.com/worksheets/e-lab_online_page.html

       The magnification written on the ocular lens (eyepiece) is ______________________
       The magnification written on the scanning objective is _____ low power ____ high power ______

       2. The total magnification using the lenses can be determined by multiplying the objective lens with the ocular lens. What is the
       total magnification of an item viewed with the:

       low power objective ____________ high power objective __________ scanning objective ____________

       3. There are two places on the microscope where light can be adjusted. The light (which can be turned on or off) and the
       ___________________________ which can be rotated to make specimens brighter or darker.

Part   4. In class, we looked at a slide with newsprint of the letter "e". Examine the images of the e slides and draw them to scale. If we
       place the e on the slide right side up, you should note that the microscope reverses the image.
B
                     SCANNING                                     LOW                                               HIGH




Part   5. Common Things - we also looked at slides marked "common things". Basically, these were preserved slides that showed
       specimens like dust, insect parts, salt crystals..etc...
C
               Specimen 1 _______________________                                 Specimen 2 _________________________




Part   6. Answer true or false to each of the statements
D
       _____ The diaphragm determines how much light shines on the specimen.
       _____ The low power objective has a greater magnification than the scanning objective.
       _____ Images viewed in the microscope will appear upside down.
       _____ The type of microscope you are using is a scanning microscope.
       _____ For viewing, microscope slides should be placed on the objective.
       _____ The three objectives are attached directly to the stage.
       _____ The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.
Conclusion Questions

1. State 2 procedures that should be used to safely handle a light microscope.




    2. Why must the specimen be centered in the field of view on low power before going to high power.




    3. A microscope has a 20 X ocular (eyepiece) and two objectives of 10 X and 43 X respectively:


         a.) Calculate the low power magnification of this microscope. Show your work.




         b.) Calculate the high power magnification of this microscope. Show your work.




    4. How does the procedure for using the microscope differ under high power as opposed to low power? Why is
         this difference important?




    5. If you are looking through a microscope at a freshly prepared wet mount and you see several perfect circles
         that are completely clear surrounding you specimen, what is the most likely explanation?