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General Microbiology, Biology 251 Fall, 2007 Syllabus Great Basin College Professor: Pete Bagley Office: Lundberg Hall 128B Phone: 753-2229 (wk); 738-1399 (hm) E-mail: email@example.com Office Hours: T & Th, 9:30-12; W, 9:30-10:45; and by appointment. Lecture Reading Assignments and Exam Dates Chapter Topic 1 Introduction 3 Microscopy 4 Functional Anatomy of Cells 5 Microbial Metabolism 6 Microbial Growth Exam I, Monday, Sept 19 7 Control of Microbial Growth 8 Microbial Genetics 9 Recombinant DNA and Biotechnology 10 Classification 11 Prokaryotes 12 Eukaryotic Microbes Exam II, Monday, Oct 15 13 Viruses 14 Disease and Epidemiology 15 Pathogenicity 16 Nonspecific Host Defenses 17 Immune Response 18 Applications of Immunology Exam III, Monday, Nov 7 19 Immune System Disorders 20 Antimicrobial Drugs 21 - 26 Selected Pathogens & Diseases 27 Environmental Microbiology 28 Applied Microbiology Final Exam, Monday, Dec 10 Reading assignments and exam dates are tentative, and may be changed at the discretion of the instructor. Any changes will be announced in class. Text: Microbiology, an Introduction. 8th ed. Tortora, Funke, & Case. Lab Manual: Understanding Microbes. Claus. Grading System and Policies: The grade for this course will be based on two components: performance in lecture; and laboratory. They will be weighted 70% for the lecture, and 30% for the lab. The lecture grade will be based on four, one hour exams, worth 100 points each. These exams are not comprehensive. The lab grade will be based on two practical exams worth 100 points each, and 5 reports worth 20 points each. Also 20 points of the lab total will be based on my technical evaluation of your proficiency in the laboratory. Staining, aseptic technique, use and care of equipment, are some of the factors that will be judged. Excessive tardiness/absences and careless behavior will also be factored into the technical evaluation. Attendance in lab is mandatory, there will be no make up labs. The grading scale is as follows: 90 - 100% = A 80 - 89% = B 70 - 79% = C 60 - 69% = D > 60% = F All exams and lab practicals will be held on the dates listed in the syllabus. Any changes to that schedule will be announced in class well in advance. Missing an exam due to personal reasons or illness must be approved in advance if possible, or by contacting me within 24 hours of the exam in the case of emergency. Make up exams will be allowed and scheduled at my discretion. Lab practicals are difficult to set up. As a result, make up lab practicals will only be scheduled in unusual circumstances. In these cases the format of the exam may change. Do not miss lab practicals! The lab quizzes will be scheduled at random throughout the semester. There will be no make ups for lab quizzes. Attendance will not be taken, but be advised: Regular attendance is the single most important thing you can do to get and maintain a good grade. This syllabus does not in any way represent a contract. It is a reflection of the intent of the instructor, but do recognize that it is an organic construct that may change as the semester progresses. Any changes will be announced in class. Lab Schedule Date__ Exercise Topic Sept 1 1 Principles of Aseptic Technique 2 Aseptic Method of Transfer 3 Microscope 4 Intro to Staining 5 Smear Prep and Simple Stains Sept 7 7 Gram Stain 8 Acid-Fast Stain 10C Bacterial Flagella and Motility 11 Bacterial Capsules Sept 14 Lab Exam I And: 12 Sterilization Principles and Methods 13 Preparing Culture Media 14 Streak Plates 15 Culture Purity 16 Agar Slants and Agar Deeps 17 Broth Cultures Sept 21 21 Selective and Differential Media 23 Effects of Temperature 24 Effects of Elevated Sugar and Salt 25 Effects of Oxygen Sept 28 26 Intro to Extracellular Degradation 27 Degradation of Polysaccharides 28B Degradation of Protein 29 Hydrolysis of Lipids 30 Differential Utilization of Citrate 31 Acid and Gas Production From Fermentation Oct 5 Lab Exam II And: 32 Methyl-Red and Voges-Proskauer Tests 34 Indole Production 35 Litmus milk 36 Catalase Activity Lab Schedule Date Exercise Topic Oct 12 58 Unknown Identification 43 Antiseptics and Disinfectants 44 Antibiotic Evaluation 45 Coagulase 46 Hemolysis of Red Blood Cells Oct 19 20 Counting Viable Cells Handout Effects of Handwashing Nov 2 Lab Exam III And: 53 Detecting Coliforms in Water Handout Bacterial Transformation Nov 9 53 Detecting Coliforms in Water 39 UV Nov 16 Hand out Eukaryotic Microbes Nov 30 Hand out DNA Dec 7 Lab Exam IV Always read the lab exercise ahead of time. Knowing the procedure allows the excise to go more smoothly and quickly. Important: Lab is scheduled on Fridays, but many exercises will require that observations be taken on cultures within 48 hours. This will require your coming into lab on Monday to make those observations. Lab Reports There will be six lab reports to complete for this course. The reports must be typed, double spaced. The reports should follow the following outline: Introduction Give the background to the topic. Why is it important? Briefly explain what you are going to do in the experiment (i.e., state the Objective of the experiment). Procedures Explain what organisms, materials and methods were used in the experiments. How did you do it? Results Present the results. Point out key or unexpected results. Conclusion What was the significance of your results? Explain the importance of the experiment, and whether or not your outcome was inconsistent with what was expected. Tables, graphs, etc. may be helpful. Do not remove pages from your manual. While this is not an English class, bear in mind that correct grammar and spelling are important, and deductions will be made for excessive mistakes in these areas. Note that some of the write ups are based on more than one exercise. This does not mean that you should write a report for each exercise, rather, the exercises chosen are thematically related. So the report should blend the topics into a single report. Assignment Date Due_______ 1 Stains and Staining Friday Sept 23 (Ex 5, 7, & 8) 2 Environmental Effects Wednesday Oct 26 (Ex 23, 24, & 25) 3 Control of Microbial Growth Friday Nov 4 (Ex 43, & 44) 4 Hand Washing Experiment Friday Nov 18 5 Identification of Unknown Friday Dec 9 (Ex 58) Extra Credit Extra credit points are available for anyone with the energy and courage to take advantage of them. Up to 20 points can be added to your lecture score by writing a research paper on one of the following diseases, and then presenting a brief, informal summary to the class. The oral presentation does not affect the grading, but is a means of sharing interesting and curious information. The paper should be approximately 4 pages long, typed, double spaced with no typos or grammatical errors. Dread Diseases: Hepatitis B or C SARS Genital Herpes AIDS/HIV Gonorrhea Syphilis Chlamydia Anthrax Influenza Yellow Fever West Nile Fever Dengue Fever Ebola/Hemorrhagic Fever Tuberculosis E. coli Food Poisoning Brucellosis ‘Mad Cow Disease’/Bovine Spongiform Encephalopathy Hanta virus Small Pox Catalog Description: BIOL 251 General Microbiology (4) A laboratory and lecture course emphasizing taxonomy, morphology, physiology, infectious diseases, and ecology of microorganisms in addition to skills in aseptic procedures, isolation, and identification. Open to all life science majors and allied health majors. Prerequisite: BIOL 190 or equivalent. Course Objectives: To provide a strong basis of microbiological knowledge including: the characteristics and classification of microbes; microbial metabolism; microbes and disease; molecular genetics; and interactions between microbes and the environment. In the laboratory component, students will acquire a working knowledge of sterile techniques, media preparation, staining, microbial metabolism, and the isolation and identification of microbes. This knowledge base will be directly applied to the analysis of a variety of hands Course Outcomes: Students will be able to apply knowledge and skills gained in a variety of situations: use of aseptic technique in clinical and laboratory environments; identification of unknown organisms; evaluation of risks associated with a variety of pathogenic microbes; comprehension of molecular genetics and biotechnology; of the basic factual content of biology, including relevant principles of chemistry, physics, and geology. Beyond this factual base, students will be expected to comprehend this information sufficiently to analyze and evaluate simple biologic situations that have not been directly explicated in lecture or laboratory. Course assessment: Students will be assessed with three distinct tools: multiple choice lecture exams; laboratory practical exams; and a written report on a laboratory experience. The lecture exams are designed to test the students ability to recall matters stated in lecture and the text, define key terms, and use concepts in a meaningful way. Laboratory practicals will asses both visual and textural recall, as well as provide the opportunity for analysis and evaluation of concepts introduced in lab with lecture information. The lab report will asses how well students have comprehended and synthesized a key laboratory experience, as well as their ability to communicate these concepts via the written word.
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