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Lymphocytes They come from the bone marrow Found only in ...

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Lymphocytes

- They come from the bone marrow

- Found only in vertebrates immune systems

- Part of the adaptive immune system

- All lymphocytes originate, during haematopoiesis from a common

lymphoid progenitor before differentiating into their distinct lymphocyte

types.

- Migrate to parts of the lymphatic system such as the lymph nodes, spleen,

thymus, tonsils, peyers patches (in intestine)

- two main types of lymphatic cells, T cells and B cells also Natural killer

cells (more important in the innate immune system)

- lymphatic system also involves a transportation system - lymph vessels -

for transportation and storage of lymphocyte cells within the body

- The lymphatic system feeds cells into the body and filters out dead cells

and invading organisms such as bacteria.

- The lymphocyte cells travel through your body until they find an antigen

of the right size and shape to match their specific receptors



How do lymphocytes differ from Phagocytic cells?

1. Lymphocytes re-circulate through the body looking for antigens while

phagocytic cells are stimulated to migrate towards the antigens along a

chemotactic gradient

2. Lymphocytes are antigen specific due to their specific surface receptors;

phagocytes eat anything foreign that they find

3. Recognition of specific antigens causes proliferation (To grow or multiply by

rapidly) or cell activity e.g. The secretion of cytokines

4. Lymphocytes maintain a memory of the antigen allowing faster second degree

response; phagocytes do not have any recognition thus they have no memory.

Therefore, their secondary response would be no quicker if they worked alone.



Natural killer T cells (NKT cells)



 A special kind of lymphocyte that bridges the adaptive immune system with the innate

immune system.

 NKT cells recognize glycolipid antigen presented by a molecule called CD1d.

 Once activated, these cells can perform functions ascribed to both Th and Tc cells (i.e.,

cytokine production and release of cytolytic/cell killing molecules).



The B cells

 B cells migrate to the spleen and mature into B lymphocytes

 When a B cell is in resting stage it has IgM and IgD on the cell wall

 The B lymphocyte cell searches for antigen matching its receptors. If it finds such antigen it

connects to it, and inside the B cell a triggering signal is set off

 When the B cell is stimulated an Ig class switch takes place and replaces IgM and IgD with

IgG as the new surface receptor

 B cell now needs proteins produced by helper T cells to become fully activated.

 When this happens, the B cell starts to divide to produce clones of itself. (This is the reason

that lymph nodes swell during an infection – B cells are splitting faster than they are leaving)

 During this process, two new cell types are created, plasma cells and B memory cells.









o The plasma cell is specialized in producing a specific protein, called an antibody that will

respond to the same antigen that matched the B cell receptor.

o Antibodies are released from the plasma cell so that they can seek out intruders and help

destroy them. Plasma cells produce antibodies at an amazing rate and can release 100

000/min for up to 5 days.

o When the Y-shaped antibody finds a matching antigen, it attaches to it.

o Opsonisation occurs

 The attached antibodies serve as an appetizing coating for eater cells such as the

macrophage.

 Antibodies also neutralize toxins and incapacitate viruses, preventing them from infecting

new cells.

 Bacteria and other pathogens covered with antibodies are also more likely to be attacked

by the proteins from the complement system.

o Memory Cells are the second cell type produced by the division of B cells. These cells have

a prolonged life span and can thereby "remember" specific intruders.

o They have IgG on their surface

 Not having to go through the class switch helps to make the secondary

response faster

T cells

o T cells are white blood cells known as lymphocytes

o All T cells originate from hematopoietic stem cell in the bone marrow.

o Play a central role in cell mediated immunity (adaptive immune system)

o T cells can also produce memory cells with an even longer life span than B memory cells.

o T cells migrate to and mature in a distinct organ, called the thymus.

o Following maturation, the lymphocytes enter the circulation and peripheral lymphoid organs

(e.g. the spleen and lymph nodes) where they survey for invading pathogens and/or tumour

cells.

o They can be distinguished from other lymphocyte types, such as B cells and natural killer

cells by the presence of a special receptor on their cell surface called the T cell receptor

(TCR).



Cytotoxic T cells (TC cells, CD8 T cells, or CTLs)

 NOTE: All nucleated cells have MHCI

 Destroy virally infected cells and tumour cells, and are also implicated in transplant

rejection.

 These cells are also known as CD8 T cells, since they express the CD8 glycoprotein at

their surface.

 associated with MHC class 1 They can look into a cell and decide to kill it or not



CD4 T Cells With MHC Class II

NOTE: Only B cells, macrophages and dendritic cells have MHC II

o In resting stage they are called TH0

 Helper T cells (TH cells)

o Once activated, they divide rapidly and secrete small proteins called cytokines

that regulate or "help" the immune response.

o Depending on the cytokine signals received, these cells differentiate into TH1 or

TH2

 TH1 produces INF gamma to activate macrophages to mature

 TH2 produces IL4 to stimulate B cells to divide and produce antibodies

o Associated with MHC class II.

 Note: if one form is activated the other form is turned off

Memory T cells

o A subset of antigen-specific T cells that persist long-term after an infection has

resolved.

o They quickly expand to large numbers of effector T cells upon re-exposure to

their cognate antigen, thus providing the immune system with "memory" against

past infections.

o Memory T cells comprise two subtypes: central memory T cells (TCM cells) and

effector memory T cells (TEM cells).

o Memory cells may be either CD4 or CD8.



Regulatory T cells (Treg cells)

o formerly known as suppressor T cells

o Crucial for the maintenance of immunological tolerance.

o Their major role is to shut down T cell-mediated immunity toward the end of an

immune reaction and to suppress auto-reactive T cells that escaped the process of

negative selection in the thymus.

o Main cytokine is IL10

o Two major classes of CD4 regulatory T cells have been described, including the

naturally occurring Treg cells and the adaptive Treg cells.



 The binding of CD 8 to MHC 1 and CD4 to MHC II ensures that Tc and TH cells interact

the way they should and perform the proper functions.



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