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PREDICT Study:

A multicenter study in Patients undergoing

anthRacycline-based chemotherapy to assess the

Effectiveness of using biomarkers to Detect and

Identify Cardiotoxicity and describe Treatment





Daniel Lenihan, MD

MDAnderson Cancer Center

CCOP Annual Meeting 2009

Daniel J. Lenihan, MD

MD Anderson Cancer Center

Houston, TX



I will discuss off label use and/or investigational use of certain

medication.



Research Support: Biosite, Inc.

Consultant: Genentech, Bayer/Onyx

Speakers bureau: None

Why discuss cardiac disease and

cancer? Let’s consider…



• These are by far the two most common disease

conditions in the developed world

• Cardiac disease may pre-exist cancer therapy or

may be caused/exacerbated by it

• Cancer therapy is more effective than ever before

at treating cancer, but has a price..

• Therapeutic choices for both cardiology and

oncology have significant overlap

These are by far the two most common disease

conditions in the developed world….

Women Men

Heart

Disease

No Heart Heart

Disease Disease

No Heart

Disease









•Lifetime risk of developing coronary heart disease at age 40 years (U.S.)





Women Men



Cancer





Cancer No Cancer



No Cancer









•Lifetime risk of developing cancer (U.S.)

American Cancer Society. Cancer facts & figures 2007,

Lancet 1999;353:89-92.

Five-year Relative Survival (%)* during Three Time

Periods By Cancer Site

Site 1975-1977 1984-1986 1996-2002

• All sites 50 53 66

• Breast (female) 75 79 89

• Colon 51 59 65

• Leukemia 35 42 49

• Lung and bronchus 13 13 16

• Melanoma 82 86 92

• Non-Hodgkin lymphoma 48 53 63

• Ovary 37 40 45 †



• Pancreas 2 3 5

• Prostate 69 76 100

• Rectum 49 57 66

• Urinary bladder 73 78 82

*5-year relative survival rates based on follow up of patients through 2003. †Recent changes in classification of ovarian cancer

have affected 1996-2002 survival rates.Source: Surveillance, Epidemiology, and End Results Program, 1975-2003, Division of

Cancer Control and Population Sciences, National Cancer Institute, 2006.

In any patient, heart disease and cancer are likely to overlap









Driver BMJ 2008:337:a2467

Why discuss cardiac disease and

cancer? Let’s consider…

• These are by far the two most common disease

conditions in the developing world

• Cardiac disease may pre-exist cancer therapy

or may be caused or exacerbated by it

• Cancer therapy is more effective than ever before

at treating cancer, but has a price..

• Therapeutic choices for both cardiology and

oncology have significant overlap

In breast cancer patients, heart disease has a

great impact….









JAMA. 2001;285:885-892

Baseline Characteristics of Breast Cancer Cohort

and Chemotherapy Subgroups









Doyle JJ et al. J Clin Oncol. 2005 Dec 1;23(34):8597-605.

Why discuss cardiac disease and

cancer? Let’s consider…



• These are by far the two most common disease

conditions in the world

• Cardiac disease may pre-exist cancer therapy or

may be caused/exacerbated by it

• Cancer therapy is more effective than ever

before at treating cancer, but has a price..

• Therapeutic choices for both cardiology and

oncology have significant overlap

www.msnbc.msn.com. Aug 2005.

Even in early stage breast cancer, cardiac

disease does matter…

• Patients with

early stage

breast cancer

are 4x more

likely to die

of non-cancer

conditions

(up to 45 %

are cardiac in

nature)









Hanrahan, et al. JCO 25: 4952-4960, 2007

Why discuss cardiac disease and

cancer? Let’s consider…



• These are by far the two most common disease

conditions in the world

• Cardiac disease may pre-exist cancer therapy or

may be caused/exacerbated by it

• Cancer therapy is more effective than ever before

at treating cancer, but has a price..

• Therapeutic choices for both cardiology and

oncology have significant overlap

Anti-VEGF Therapy can decrease blood

flow resulting in cancer control









Willitt, JCO 2006

You are only as good as your endothelium (or your

small vessels…)









Kirchmair R. Circulation. 2005 May 24;111(20):2662-70.

Systemic Effects of Anti-VEGF Therapy

Tumor Tissues Normal Tissues

(VEGF upregulated) (VEGF constitutively expressed)









Hypertensive remodeling

Lung cancer (bevacizumab) Microvascular rarefaction

Inhibition of tumor growth, tumor cavitation Cardiomyopathy (sunitinib and sorafenib)









Hepatocellular carcinoma (sorafenib)

1 2 3

Tumor necrosis









Microcirculation: 1. normal arteriole, 2. functional rarefaction

(endothelial dysfunction,vasoconstriction), 3. anatomic rarefaction

Renal cell carcinoma (sunitinib)

Tumor shrinkage, tumor cell necrosis





Thrombotic microangiopathy

Glomerulopathy / glomerulonephritis

Proteinuria

Hypertensive nephropathy

Colorectal cancer (bevacizumab)

Deceleration of tumor growth

efficient chemotherapy delivery

How Accurate is Clinician Reporting

of Chemotherapy Adverse Effects?









Journal of Clinical Oncology, Vol 22, No 17 (September 1), 2004: pp. 3485-3490

How Accurate is Clinician Reporting of

Chemotherapy Adverse Effects?



• Comparative study of patient reporting of

eight symptoms with physician reporting of

same symptoms

• Physician Sensitivity=47%

• Physician Specificity=68%



JCO 2004 22:3485-3490

Classic Triad of Heart Failure



• Dyspnea



• Lower extremity edema



• Fatigue

Difficulties in diagnosing “heart

failure”

• Can be a wide range of presentations

• Many of the symptoms of heart failure

overlap with other disease states such as

COPD, Obesity, Nephrotic Syndrome, Drug

induced Edema, Cirrhosis, Sleep Apnea,

and Cancer

• How to effectively and efficiently

differentiate between these entities?

BNP guided therapy for cardiac disease

(eg. HF) is very useful and appears to change the

outcome….









Kaplan-Meier curves examining time to first event of the primary clinical endpoint showed a clear divergence between the

groups by 6 months (p=0·034) and remained significant when reanalysed to include only heart-failure events or death

(p=0·049).

Troughton et al. Lancet. 2000: 355, 1126-30

Principles for the Management of Cardiac

Disease Benefit Cancer Patients

• Biomarkers used in Cardiology are also used in

Oncology

• Cardiac specific therapy allows for more effective

cancer treatment

There is significant reversibility of LV

dysfunction with trastuzumab-related cardiac

toxicity









Ewer, et al Journ of Clinical Oncology 2005,23;p 7820-6.

Recovery of LV dysfunction with standard

HF therapy









Jensen, et al. Annals of Oncology. 2002. 13:499-709.

Significant Improvement in EF After Optimal HF Therapy





100

100









75

Percent of Patients







55



50









25

14







0

LVEF HF with EF Improved

Decrease Normal EF After Optimal

After Chemo Treatment





Lenihan et al, HFSA 2008

Carvedilol appears protective during adriamycin based

chemotherapy









Data expressed as mean values.

Kalay et al. JACC. Dec 2006. 48:2258-62

ACE Inhibition appears quite important

for prevention of toxicity









Cardinale D et al. Circulation. 2006;114:2474-2481

Troponin I is valuable in detecting Cardiotoxicity









Cardinale et al. Circ. 2004;109:2749-2754

BNP, a marker of volume overload, may also be an

effective marker of subsequent myocardial

damage





No HF Developed HF









Okumura et. al. Acta Haematologica. 2000. 104:158-163.

How do we best detect cardiotoxicity by Echo?









Sa = longitudinal (annular) systolic contraction, E = transmitral E wave velocity, A = transmitral A wave velocity,

Ea = longitudinal (annular) early diastolic relaxation velocity. *P 600 mg/m2 5.4



Doxo bolus > 550 mg/m2 10



Doxo 1000 mg/m2 20



Doxo 500 mg/m2

7

0 5 10 15 20 25

CHF (%)

Hensley ML et al J Clin Oncol 1999; 17(10):3333-

Study Timeline



Cycle 1 2 3 4 5 6

Weeks (approximate) 0 3 6 9 12 15 18 24

BNP x x x x x x x x

TROPONIN x x x x x x x x

EF (by Echo) x x x+

Physical Exam x x x x x x x x

ECG x x x+

MDASI x x x x

Baseline End

+if clinically indicated

TABLE 1. BASELINE DEMOGRAPHICS

Number of patients=109 %

Gender (Male/Female) 48 / 52

Age (years ± std dev) 56 ± 14

Cancer Diagnosis

Breast 10

Sarcoma 55

Lymphoma 32

Other 3

Cardiac Diagnosis

Coronary Artery Disease 10

Prior Myocardial Infarction 4

Risk Factors

Diabetes 14

Family History of Early Heart Disease 20

Hypertension 50

Hyperlipidemia 32

Obesity 35

Smoking 11

Cardiac Medications

Beta Blocker 22

Ace Inhibitor 17

ARB 13

Statins 23

Aspirin 10

Antiplatelets 6

Summary of Cardiac Events (Pilot Data)

Results:





• The only factors significantly associated

with cardiac toxicity included older age,

history of MI and elevated BNP

Factors associated with having a cardiac

event during the study period



Cardiac No Cardiac

Event Event

Label Value Total P



n (%) n (%)



Previous Myocardial Infarction Yes 2 50 2 50 4 0.0500



1 BNP over 100 before event Yes 11 22 40 78 51 0.0001



1 BNP over 150 before event Yes 11 37 19 63 30 100 109 100 (72, 100) 59 (49, 69) 22 (11,35) 100 (94,100)







1 BNP > 150 109 100 (72, 100) 81 (71, 88) 37 (20, 56) 100 (95, 100)







1 BNP > 200 109 91 (59, 100) 90 (82, 95) 50 (27, 73) 99 (94, 100)







EF15% 102 30 (7, 65) 84 (75, 91) 17 (4, 41) 92 (84, 97)







All data expressed as percent (95% Confidence Interval)

Rationale

• Troponin and BNP markers are reliable,

noninvasive and simple to measure.

• Early identification of LV dysfunction

would stratify patients needing adjustments

in their chemotherapy or who may benefit

from concurrent use of cardioprotective

agents (e.g. beta blockers or ACE

inhibitors).

Statistical Considerations

• The study by Cardinale et. al. notes a 15-

20% incidence of cardiotoxicity from high-

dose chemotherapy.

• Our Pilot data shows an incidence of at least

10% for cardiotoxicity from all

anthracycline regimens.

Inclusion Criteria

• Patient age 18-85 years

• Starting a new course of chemotherapy

that includes an anthracycline (does not

have to be first-line therapy and previous

anthracycline use is allowed)

• Has a life expectancy greater than 12

months

Exclusion Criteria

• Unstable angina within the last 3 months

• Myocardial infarction within the last 3

months

• LVEF less than 40%

• Patients receiving concurrent dexrazoxane

• Decompensated HF in the last 3 months

Cardiac Event

• Any new symptomatic cardiac arrhythmia

• Acute coronary syndrome

• Symptomatic HF

• Development of asymptomatic left ventricular

dysfunction (defined as LVEF less than 50 %

with a normal baseline or a decrease of greater

than 15% from baseline)

• Sudden cardiac death (defined as rapid and

unexpected death from cardiac causes with or

without known underlying heart disease)

Univariate logistic regression modeling the

probability of having a cardiac event

(Pilot data)

Summary of Cardiac Events (Pilot Data)

PREDICT Study

Equipment Features for Biomarker

Testing

• Built in quality control features

• Simple one-step testing

• Accurate

• Results in 15 minutes

• Low maintenance

Conclusion

• The ability to predict and identify patients

who develop cardiotoxicity with

chemotherapy needs improvement

• Biomarkers may actually identify those

patients long before heart failure is present

• Establishing a method to easily and reliably

detect cardiotoxicity can have a profound

impact on outcomes

Supported in part by the Lance Armstrong Foundation


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