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carcass and meat quality

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					research activities and methods used by Nguyen Van Hau, [pigs]

1. Reproductive performance of Mong Cai and Ban sows
1.1 data recording by farmers and data recording and crosschecking by researchers
1.2 data storage in PigChamp software (Version 2.0, University of Minnesota, St Paul, MN, 1985)
- comparison of reproductive performance between Mong Cai and Ban sows
- reproductive performance of Mong Cai sows
- factors influencing reproductive performance of Mong Cai sows
- reproductive performance of Ban sows

2. Growth performance of Mong Cai and Ban pigs
2.1 bi-monthly individual weighing by electronic weight scale
2.2 data recording of weight, age, year, parity, and length of measurement period (interval between two measurements) of each p
- factors influencing growth performance
- growth performance of Mong Cai and Ban pigs

3. Carcass characteristics of Mong Cai and Ban pigs
3.1 weighing prior to slaughter by weighing scale
3.2 backfat thickness measurement by ultrasound lean meter (Renco, USA)
3.3 weighing and recording of whole carcass
3.4 removing and weighing of leaf-fat of the kidney
3.5 weighing and recording of hot carcass
3.6 weighing and recording of dissected main parts (ham, shoulder, loin, and belly) and parts of lower value (head, legs, tail and kid
3.7 weighing and recording of bones, muscular tissue (lean meat), fatty tissue and skin of each part
- factors influencing carcass characteristics
- carcass characteristics of Mong Cai and Ban pigs

4. Meat quality of Mong Cai and Ban pigs
4.1 drip loss determination of fresh meat by the bag method
4.2 assessment of muscle pH of meat at 45 minutes (pH45) and 24 hours (pH24) post-mortem with pH meter (Matthäus, Pöttmes,
4.3 measurement of electric conductivity of meat by LF-Star (Matthäus,Pöttmes, Germany)
4.4 meat colour determination by Opto-Star measuring instrument (Matthäus, Pöttmes, Germany)
- factors influencing meat quality
- meat quality of Mong Cai and Ban pigs

5. Relationship of selected genes with reproductive performance of Mong Cai and Ban sows
5.1 collection of ear tissue samples
5.2 DNA extraction and purification according to the standard protocol using protein kinase digestion followed by phenol/chlorofo
5.3 measurement of DNA dilution in spectrophotometer UV/visible light and calculation of DNA concentration
5.4 polymerase chain reaction (PCR) to amplify selected loci (ESR, OPN, COX2, EGF, PRLR)
5.5 detection of restriction fragment length polymorphisms (RFLPs) of ESR, OPN, COX2, EGF and PRLR by electrophoresis
- genotyping
- allele frequency
- genotype frequency
- effect of selected loci on reproductive performance of Mong Cai and Ban sows
 (interval between two measurements) of each pig




 ) and parts of lower value (head, legs, tail and kidneys) according to the EU reference dissection method




post-mortem with pH meter (Matthäus, Pöttmes, Germany)




ein kinase digestion followed by phenol/chloroform extraction and precipitation with isopropanol
ulation of DNA concentration

COX2, EGF and PRLR by electrophoresis
Research activities by Nguyen Van Hau, [sows]
Son La and Mai Son district, Son La Province

sample size reproductive data
System                                      Demand driven                     In transition
Village                            Ban Bo               Ban Buon   Ot Luong    Na Huong
               Mong Cai sows         27                    29          -            21
Breed
               Ban sows               -                     -          -            17
totalvillage                         27                    29          -            38
In transition                   Resource driven
 Na Huong       Bo Duoi   Pa Dong             Tong Tai A   totalbreed   NoLitters
      21          15         -                    -           92         281
      17          34         -                    -           51         114
      38          49         -                    -          143         395
Research activities by Nguyen Van Hau, [fatteners]
Son La and Mai Son district, Son La Province

sample size growth performance determination
System                              Demand driven    In transition   Resource driven
           Mong Cai fatteners           93                177               -
Breed
           Ban fatteners                 -                n.a.             49
totalbreed   Noweight
    270         492
    178         407
Research activities by Nguyen Van Hau, [barrows]
Son La and Mai Son district, Son La Province

sample size castrated pigs
for carcass character-istics
and meat quality
determination                           Variables determined
            breed                       Carcass characteristics
  Mong Cai          Ban                     Carcass length (cm)
      10            10                      Hot carcass weight (kg)
                                            Hot carcass dressing (%)
                                            Cold carcass weight (%)
                                            Cold carcass dressing (%)
                                            Backfat thickness (mm)
                                            Loin eye area (cm2)
                                            Lean meat content (%)
                                            Fat content (%)
                                            Shoulder yield (kg)
                                            Ham yield (kg)
                                            Loin yield (kg)
                                            Belly yield (kg)
                                        Meat quality traits
                                            drip loss1 (%)
                                                 1
                                            pH45
                                                      1
                                               pH24
                                               LF451
                                               LF241
                                                      1
                                               OP24
                                        1
                                            at longissimus dorsi
Research activities by Nguyen Van Hau, [sows]
Son La and Mai Son district, Son La Province

sample size of ear tissue samples of sows for candidate gene determination and reproductive data
System                               Demand driven                             In transition
Village                         Ban Bo         Ban Buon         Ot Luong        Na Huong           Bo Duoi
Breed          Mong Cai           24              22               11               21               11
               Ban                 -               -               20               11               16
totalvillage                      24              22               31               32               27
      Resource driven
Pa Dong         Tong Tai A   totalbreed   NoLitters
    -                -          89         341
   18               12          77         172
   18               12         166         n.a.

				
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posted:11/18/2011
language:English
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