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Algal concentrates in hatchery culture

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Algal concentrates in hatchery culture
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Culture of uni-cellular algae is a pre-requisite for successful operations in hatchery practices for shrimp, crab and most fish.

November | December 2011

Feature title: Algal concentrates in hatchery culture



International Aquafeed is published five times a year by Perendale Publishers Ltd of the United Kingdom.

All data is published in good faith, based on information received, and while every care is taken to prevent inaccuracies,

the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of

information published.

©Copyright 2009 Perendale Publishers Ltd. All rights reserved. No part of this publication may be reproduced in any form

or by any means without prior permission of the copyright owner. Printed by Perendale Publishers Ltd. ISSN: 1464-0058









The International magazine for the aquaculture feed industry

F: Algal concentrates Aquaculture UK 2012 F: Algal concentrates

Such algae can then be concen- 23-24 May 2012 Feeding robots

trated via centrifuge then packaged

and stored prior to use.

Aviemore, Scotland

"Anectodal evidence Single feeders

Such a system is flexible in terms suggests that Zoea 2 Centralised feeding

of algal composition in that formula-

tions for fish and shrimp larvae can Syndrome commonly

be tailored to meet the require-

ments of the larvae.

experienced in

These concentrates are easily many hatcheries is

stored and applied to tanks and can

also be used to enhance and enrich nutritional in origin

living feeds such as rotifers and

Artemia nauplii. Its use reduces

but complicated by

the need for mass culture tanks secondary invasion

which can then be turned over to

larval and nursery rearing and also by bacteria or viruses

reduces demand on labour time and







Algal concentrates

equipment.

(Li. Pers. Comm.)"

Larval quality is improved and

development is accelerated, result- Professional Feeding Technology







in hatchery culture

ing in healthier, stronger larvae • Suitable for many types of fish farms

which can be sold at a premium, • Robot feeding unit for up to 240 tanks

thereby improving returns on • Most accurate feeder ever made

investment. • Centralised pipe feeding system

The UK’s major Aquaculture exhibition and conference • Export/import for production size was five tonne and stocking

Shrimp ZM) as food sources for larvae of group, tank

by John S Clark PhD, Aquatic Animal Health and Nutrition Technical Consultant, Bangkok, Thailand featuring the latest aquaculture products and innovations. white shrimp (Litopenaeus vannamei) in

Materials and methods the management systems density at nauplius was 200/litre. As well

Visit www.aquacultureUK.com for further information or

A trial was run comparing live Thailand. as their conventional feeds the test groups

contact info@aquacultureUK.com

Chaetoceros with a commercially available There were three control our website www.arvotec.fi Shrimp ZM two times/day

See treatments were fed on

algal concentrate (Meridens Phyconmmix and three test treatments in each study to Mysis 3 and then three times/day to







Fish_Farming Int_1/8_107X146.indd 1

Your partner in technology, 17/10/2011 12:45



equipment and plants for animal-









C

Good

and aqua feed and petfood.

ulture of uni-cellular composition; the major expense in algal nutrient requirements would seem a logical Agricultural

algae is a pre-requisite culture comes in the nutrient component. progression. Said species of algae can be Practice

for successful operations Algae can require up to 17 different grown in a sterile, hermetically sealed, V AI C UI U M C O A T IE R

I I I I

New Delhi Mexico City Cape Town Sao Paulo Cairo Warsaw Atlanta Bangkok



in hatchery practices for trace components in their culture medium. bio-secure environment, then harvested at

shrimp, crab and most fish. The cost of production is further pre-determined times during the log phase

Providing proficient tools to achieve cost-effective

increased due to the requirement for of growth to optimize nutrient quality and and sustainable aquaculture practices

Problems with algal culture and inherent specialist technicians. Large-scale culture consistency.

disadvantages are described here, with their increases the likelihood of

advantages in many cases being overcome breaches in bio-security, as

by these disadvantages. The current study pathogens can easily be trans-

describes a novel, industrialised approach mitted from nearby culture

to negate these disadvantages and to place tanks or via inadvertent intro-

hatchery culture methods on a more con- duction of insects etc.

sistent and stable platform. Generally speaking, only one Coming Your Way

This strategy was designed and imple- algal species tends to be cul- Everything you need to know about ECOBIOL Aqua

mented by Meriden Animal Health of the tured per single farmed species, Good Agricultural Practice Certification a highly effective single strain probiotic

UK and is presented in the form of the therefore nutrient composition at a place near you!

Phyconmmix range of products. becomes a critical factor espe- GUSTOR Aqua

cially when algae is entering its Register now on www.tour2011.org a natural growth promoter

The important factors decline and death phases, and

It is estimated that over 40 species of composition can therefore vary

Meet us in one of the following cities:

Would you like to know more about the

BIOMET Aqua

New

 Delhi – India, 1/2 March 2011

uni-cellular algae are in use in the aquacul- widely.  Mexico City – Mexico, 11/12 April 2011 GLOBALG.A.P TOUR 2011? an organic mineral source

ture sector.They are generally recognised as Since single species algae  Town – South Africa, 11/12 May 2011

Cape Then please see www.tour2011.org or contact

 Paulo – Brazil, 6/7 June 2011

Sao Nina Kretschmer: kretschmer@globalgap.org

difficult to grow in mass culture, particularly cannot provide all of the nutri- Follow us on Twitter@GLOBALGAP!

in low light (cloudy) or rainy conditions. ents required by larval shrimp

 Cairo – Egypt, 12/13 July 2011

Wynveen Poland, 14/15 September 2011

Warsaw – International b.v. Tel: +31 (0)26 - 479 06 99 rl

Natuna trition

 u

The most important factors to consider in and fish, careful selection of a  Box USA,

P.O.Atlanta –38 17/18 October 2011 Fax: +31 (0)26 - 479 06 98 NOREL,S.A. • Jesús Aprendiz, 19, 1º A y B • 28007 Madrid (SPAIN)

algal culture are temperature, salinity, pH, range of algal species which Bangkok – Thailand, 22/23

6666 ZG Heteren November 2011

 info@wynveen.com Hosted by: 501 40 41 • Fax +34 91 501 46 44 • www.norel.es

Tel. +34 91

California Aquaculture Association

light intensity, photoperiod and nutrient cover the spectrum of larval The Netherlands www.wynveen.com





30 | InternatIonal AquAFeed | november-December 2011

10 november-December 2011 | InternatIonal AquAFeed | 31

11

AN. REV. 90X132.indd 1 02/08/11 13:13

F: Feature

than a single species diet and this will aid in

the protection of target animals.

The improved water quality using

concentrates may simply be a function

of improved shrimp larval health lead-

ing to improved vigour and appetite; it

was noted feed consumption rates were

higher but were non-detrimental to the

larval environment. In such an environ-

ment and with a much more complete,

nutritionally balanced dietary regimen,

it is not surprising that survival, growth,

feed intake and resistance to stress are all

significantly improved.

The developmental and structural

advantages seen in the harvested post-

larvae are of considerable interest to grow

out farmers. In Plate 1 an examination of

PL15 following the manufacturers feeding (see Figure 2) and significantly heavier (see the carapace by SEM reveals the carapace

instructions. Figure 3) than controls, which results in a to be thin and pliable, and therefore more

Survival, length, weight, length/weight significantly more favourable length/weight prone to damage. By contrast, the carapace

ratio, gut/muscle ratio, feed consumption, ratio (see Figure 4). of animals fed on concentrate appears much

hepatopancreatic Vibrio count, formalin Similarly, feed utilisation seems superior denser and stronger, and would therefore

stress test were all recorded during this in that the gut/muscle ratio favours muscle be capable of resisting much more handling

trial. Scanning Electron Microscope studies in the test groups (see Figure 5) and this is stress. Such considerations are of vital

of the harvested post-larvae were also manifested as an increased Artemia nauplius importance to farmers.

conducted. and flake feed consumption (see Figures 6 Even in the case of the compound eye,

The accumulated data allowed for a and 7). the eye seems incomplete in the controls

return on investment calculation to be The final post-larvae are significantly (Plate 3) and this will of course impact on

stronger and more resistant to for- feeding behavior. It seems in general the

malin stress as evinced by survival concentrate fed animals are more devel-

rates displayed in Figure 8. Scanning oped than controls (Plate 4) and therefore

Electron Microscope studies show more suited to the rigours of pond life.

significant differences in exo-skeletal Advantages are therefore not restricted

structure and strength (Plates 1 and to simple survival and growth; there are

2) as well as in development rate of many subtle advantages that may escape

the compound eye (Plates 3 and 4). direct attention but become evident on

Such differences in stress survival deeper study.

and the structural improvement in This developmental advantage is also

the animals fed concentrates has observed in trials with concentrates in the

significance to the grow-out phase of

farming operations.

Finally, cost and return on invest-

nutrition of larval fish. In a recent study

on larval sea bass (Lates calcarifer) apart

from the advantages in terms of survival

Amino Acids

ment figure prominently in the and growth, significant developmental accel- Headquarters Choosing the right nutrients for your Aquafeed

made between the use of live and concen- vocabulary of any hatchery operator. It can eration was observed in development of Evonik Industries AG

Health & Nutrition

trated algae during larval shrimp culture. be seen from Table 1 that use of concen- dentition (Plate 5), taste bud (Plate 6) and feed additives Our amino acids help to

trates does in fact lead to more profitable buccal microbial flora (Plate 7). Rodenbacher Chaussee 4 •  replace  costly  and  scarce  raw  materials 

Results production of post-larvae and that this, When factors such as ease of storage, 63457 Hanau-Wolfgang, Germany

•  improve  protein  balance  and  production  efficiency 

phone +49 6181 59-2256

By Zoea 2 an obvious size difference was coupled with the many advantages relating ease of use, nutritional consistency, absence fax +49 6181 59-6734 •  reduce  environmental  pollution

noted (Li, pers. Comm.). to ease of use make algal concentrates very of potential pathogens, reduced labour cost

At the same time, controls experienced attractive to hatchery operators. and freeing of tank space are considered Europe & Middle East Africa +49 6181 59-6766

Latin America +49 6181 59-6761

an outbreak of Zoea 2 Syndrome and a alongside the aforementioned performance feed-additives@evonik.com | www.evonik.com/feed-additives

North America +1 678 797-4300

significant mortality occurred. It was note- Discussion superiority, the future of algal concentrates Asia North +86 10 85 27-6400

worthy that water quality in the test tanks Anectodal evidence suggests that Zoea 2 as a significant tool in hatchery culture Asia South +65 6890-6861



was viewed as superior to that observed Syndrome commonly experienced in many seems secure.

in live controls. This has been mirrored in hatcheries is nutritional in origin but com-

other trials (Pota, pers comm.; Somhathai, plicated by secondary invasion by bacteria Acknowledgements

pers.comm). or viruses (Li. Pers. Comm.). This study, in The author would like to thank all

Figure 1 shows mean survival in the which no outbreak was experienced in the concerned for their support during the

three concentrate treatments to be sig- test groups, would tend to support that development of the products and those

nificantly higher than in the live controls. premise. The nutritional profile of a multi- involved in the demonstration of the prod-

Animals are significantly greater in length algal species diet will be more complete uct performance.





18 | InternatIonal AquAFeed | november-December 2011

32 november-December 2011 | InternatIonal AquAFeed | 33

LINKS

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