Marine based protein hydrolysate improve the performance of feeds containing a low level of fish meal

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					                                     November | December 2011
Feature title: Marine based protein hydrolysate improve the performance of feeds
                         containing a low level of fish meal
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        The International magazine for the aquaculture feed industry
 F: Protein hydrolysate                                                                                                                            Table 1: Feed formulations
                                                                                                                                                                                                                            FM5 +
                                                                                                                                                                                                                                                                 F: Protein hydrolysate
                                                                                                                                                             Raw materials           FM5          FM10         FM20
                                                                                                                                                                                                                                    the feed containing the
                                                                                                                                                                                                                                                                        Amino acids profile of a Marine
                                                                                                                                                                                                                                    highest level of fish meal
                                                                                                                                                                                                                                                                        hydrolysate and a Fishmeal LT
                                                                                                                                                                 Fish meal LT         5.00        10.00         20.00      5.00
Marine based protein hydrolysate
                                                                                                                                                                                                                                    (FM20).                             (% of product)
                                                                                                                                                         Marine hydrolysate           0.00          0.00         0.00      5.00         This gain is explained
                                                                                                                                                           Corn gluten meal         17.00         15.00         12.00    15.00      by an improvement of the
improve the performance of feeds containing a low level of fish meal                                                                                     Wheat gluten meal          19.71         18.92         13.00    18.92
                                                                                                                                                                                                                                    feed utilisation compared
                                                                                                                                                                                                                                    to the treatments with no
                                                                                                                                                             Rapeseed meal          11.15           9.00         7.00      9.00
                                                                                                                                                                                                                                    hydrolysate (FM5). In the
by V Fournier, M Herault, M Hervy, Aquativ, ZA du Gohélis
                                                                                                                                                       Soyben meal (48 CP)          17.00         15.00         13.00    15.00      same way, the substitution
56250 Elven, France, Email:
                                                                                                                                                                Wheat meal          10.00         12.83         17.68    12.83      of five percent fishmeal

                                                                                                                                                              DL methionine           0.57          0.53         0.47      0.53     by five percent of hydro-
              uring the last 20 years,            Materials and methods                                                                                           Lysine HCL          1.58          1.44         1.09      1.44
                                                                                                                                                                                                                                    lysate in the treatment
              fishmeal and fish oil                   Three diets have been formulated                                                                                                                                              FM10 resulted in a better
                                                                                                                                                    Monocalcium phosphate             2.48          2.08         1.19      2.08
              replacement has become              to contain a graded level of fish meal                                                                                                                                            feed utilisation but not
              a major concern for the             (five percent, 10 percent and 20                                                                                     Fish oil     13.20         12.88         12.26    12.88      significant.
aquafeed industry to anticipate the               percent, corresponding to diets FM5,           Figure 1: Feed intake in European seabass                             Premix         2.31          2.31         2.31      2.31         If the marine hydro-
                                                                                                 fed the dietary treatments for 56 days
exceptional aquaculture growth and                FM10 and FM20 respectively) while                                                                                                                                                 lysate and fishmeal have
the lack of marine raw materials                  meeting all the dietary requirements                                                               intake was not significantly influenced by                   very closed amino acid profiles (see Figure
availability forecasted for the coming            for European seabass (see Table 1). A                                                              the feed treatments (see Figure1).                           3), the analysis of the protein quality
years.                                            fourth diet (FM5 + 5%MH) was formu-                                                                    After 56 days of trial, the growth per-                  of both raw materials showed that the
                                                  lated from the diet FM10, five percent                                                             formance was significantly decreased (see                    soluble protein and peptide contribution           Conclusions
    The dietary level of fishmeal remains a       powder marine hydrolysate replacing                                                                Figure 2) in fish fed the feed containing the                are higher for the marine hydrolysate (see            Protein hydrolysates represent key
key driver of the feed performance, affect-       five percent fishmeal, both raw materi-                                                            lowest level of fishmeal (FM5).                              Figure 4).                                         ingredients to sustain the development
ing feed palatability and feed utilization.       als having the same nutritional profile                                                                This result is mainly explained by a signifi-               The dietary inclusion of a high level of        of highly substituted fish meal feeds. It
    Year after year, the level of fishmeal        (CP: 65 percent protein; crude fat: 12                                                             cant deterioration of the feed utilisation while             peptide had a clear positive effect on the         could enhance significantly the feed nutri-
in feeds designed for carnivorous species         percent). All the feeds had the following                                                          the dietary fishmeal decreased (see Figure 3).               feed utilization by the animal, demonstrat-        tional value, providing a high level of
tends to decrease and is closed to reach a        specifications: crude protein: 46±0.5                                                              Interestingly, the application of five percent               ing the importance of the protein molecu-          essential small soluble compounds not
critical level for growth and feed utilisation.   percent, crude fat: 16±0.5 percent,                                                                marine hydrolysate allowed restoration of the                lar weight profile in the performance of           found in most of the non marine origin
Plant and land-based feedstuffs are now           crude energy: 4900±50kcal/kg.                  Figure 2: Growth performance in European            growth performance at the same level than                    raw materials and finished feeds                   raw materials.
commonly used in aquafeed formulations                The trial was conducted in the             seabass fed the dietary treatments for 56 days
with success as an alternative to fishmeal        experimental flow-through facilities
but many studies have shown that balancing        of IFREMER, centre de Brest, France.
the dietary amino acid profile of plant           Seawater (salinity: 35g/l) was filtered
rich diets to meet the fish amino acid            (high pressure sand filter) and ther-
requirements is not enough efficient to get       moregulated (water temperature: 20
satisfying fish and feed performances.            ± 1°C). Triplicate groups of 40 juvenile                                                                                                                                                          Improvement
    Fish are highly responsive to the low         European seabass (initial mean body
molecular weight nitrogen compounds               weight: 7.6±0.1g) were reared in 24                                                                                                                                                                 by nature
coming from marine raw materials. They            tanks of 80 one capacity (flow rate:                                                                       Natural (hydrolyzed) proteins
stimulate feed intake, growth and immune          three litres per minute; photoperiod:
system.                                           12 hours light: 12 hours dark).                Figure 3: Feed conversion ratio (FCR)                      for aqua feed
                                                                                                 in European seabass fed the dietary
    Plant-based and land-based raw materi-            Three tanks were allotted at ran-
                                                                                                 treatments for 56 days
als are poor in these highly soluble com-         dom to each diet.                                                                                         Whether you’re looking for a natural EU and USDA approved pellet binder for your
pounds which could explain their lack of              The diets were distributed in                                                                         production of stable pellets or nature’s best alternatives for fish meal, you can contact Sonac.
performance when used at a high level of          excess to the fish, by automatic feeder                                                                   Sonac produces valuable and essential ingredients for the production of aqua feed.
incorporation in diets. A solution to balance     (Arvotech, Finland), 10 times a day, for
the level of soluble compounds and the feed       56 days.                                                                                                  • Pro-Bind Plus a nutritional, gelatin based pellet binder, especially for pelleted (shrimp) feed.
protein molecular weight profile would be             Uneaten feed were collected every                                                                     • Gelko an ideal combination of hydrolyzed proteins and fat (68% protein, 18% fat).
the incorporation of an adequate level of         day by home-made feed waste collectors,
                                                                                                                                                            • MucoPro high content of hydrolyzed proteins, amino acids and peptides (>70% protein).
marine based protein hydrolysate.                 pooled and kept frozen till the end of the
                                                                                                                                                            • Hemoglobin Powder high protein content and good digestibility, for better feed conversion.
    Aquativ is specialised in the production      trial. Fish were counted and weighted
of protein hydrolysate from raw materials         at the beginning of the trial, every four       Figure 4: Level of soluble protein and
from different origin through controlled          weeks and at the end of the trial. Before       protein molecular weight profile (MW,
enzymatic hydrolysis. The core expertise of       every weighing, fish were fasted for 24         % of soluble protein) of fishmeal and
Aquativ makes possible to closely control         hours. Survival was daily checked. Dead         marine hydrolysate
the size of peptides generated during the         fish were counted and weighed.
process to reach the highest performance.             Over the growth period, the fol-             test. Differences were considered significant
    Many studies have been conducted by           lowing parameters were followed and              at P<0.05.
Aquativ to demonstrate the effectiveness          calculated: survival, specific growth rate,
of the application of hydrolysate at graded       voluntary feed intake and feed efficiency.       Results and discussion
dietary level of fish meal. Some results are          All data were analyzed by one-way analy-       No significant difference in fish survival
presented below.                                  sis of variance (ANOVA) followed by Fisher       was noticed at the end of the trial. Feed

                                            20 InternatIonal AquAFeed november-December 2011
                                            20 || InternatIonal AquAFeed || november-December 2011                                                                                                          november-December 2011 InternatIonal AquAFeed 21
                                                                                                                                                                                                            november-December 2011 || InternatIonal AquAFeed || 21
                                                                                                                                                        Photograph © Fred Jonny Hammeroe
Welcome to ISFNF 2012                                                                            in Molde, Norway

          4-7. June 2012
                                                       Welcome to Molde in 2012 for the 15th consecutive
                                                       International Symposium of Fish Nutrition and Feeding

  Key areas:

  ■ Sustainable resources

  ■ Health, welfare and ethics

  ■ New methods and working-tools

                                                                                                                                                                                                                                          Amino Acids
  ■ Feed technology and feeding regime

  ■ Nutrigenomics

  ■ ‘omics’ data and system biology models                                                                                                                                                 Headquarters                                   Choosing the right nutrients for your Aquafeed
                                                                                                                                                                                           Evonik Industries AG
  ■ Nutritoxicology                                                                                                                                                                        Health & Nutrition
                                                                                                                                                                                           feed additives                                 Our amino acids help to
                                                                                                                                                                                           Rodenbacher Chaussee 4                         •  replace  costly  and  scarce  raw  materials 
  ■ Nutrition in whole life cycle
                                                                                                                                                                                           63457 Hanau-Wolfgang, Germany
                                                                                                                                                                                           phone +49 6181 59-2256                         •  improve  protein  balance  and  production  efficiency 
                                                                                                                                                                                           fax +49 6181 59-6734                           •  reduce  environmental  pollution
                                                                                                                                                                                           Europe & Middle East Africa +49 6181 59-6766
                                                                                                                                                                                           Latin America +49 6181 59-6761
                                                                                                                                                         Photograph © Terje Rakke

 Some conference ingredients:                                                                                                                                                                                                    |
                                                                                                                                                                                           North America +1 678 797-4300
                                                                                                                                                                                           Asia North +86 10 85 27-6400
 ■ Get updated on the latest research on fish nutrition and                                                                                                                                 Asia South +65 6890-6861

 ■ Mingle with the 500 leading fish nutritionists in the world

 ■ Pre symposium tour to visit industry

 ■ 4 post symposium sight seeing tours in the beautiful                                             Nofima will host the event along with the Aquaculture
   coastal fjords                                                                                   Protein Centre (APC), the National Institute of Nutrition
                                                                                                    and Seafood Research (NIFES), the Norwegian
                                                                                                    School of Veterinary Science (NVH) and the
                                                                                                    Norwegian University of Life Sciences (UMB).

Please submit your abstract at by 20th January 2012
                                          18 | InternatIonal AquAFeed | november-December 2011
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Description: During the last 20 years, fishmeal and fish oil replacement has become a major concern for the aquafeed industry to anticipate the exceptional aquaculture growth and the lack of marine raw materials availability forecasted for the coming years.