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Guidelines
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Vector control issues and

new technologies

Dr Pierre Guillet

Global Malaria Programme

Vector Control and Prevention





6th Intercountry meeting of National Malaria

Programme Managers

Cairo, 3 – 6 July 2006

Vector control and prevention objectives



 Vector control to be expanded using ITNs and IRS

 Long-Lasting Insecticidal Nets to be used

– Obviate the need for re-treatment campaigns

– Cheaper than conventionally treated nets

 Targeting full coverage as an immediate objective to

protect all populations at risk of malaria

– 1 LLIN for 2 persons at risk of malaria

– Simpler to achieve

– Plans for replacement of LLINs once exhausted





Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

2 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

What is a LLIN?

 WHO definition

– 20 standard washes

– 3 years



 2 recommended products

– Olyset & PermaNet 2.0



 Life-time of a LLIN

– Polyester: 3 years

– Polyethylene: 5 years





Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

3 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Olyset efficacy



 Several trials concluded to good efficacy for 5 up to 7 years.

 On going Tanzania multi-village trial: almost complete interruption

of transmission with both Olyset & PermaNet for more than 18

consecutive months



 Efficacy against leishmaniasis vectors confirmed

 CDC results: rapid loss of efficacy based on WHO test cone and

slow regeneration after washing.

– Need for standardized test methods and procedures

– Difficulty in testing efficacy of permethrin treated materials



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

4 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

WHO test cones (3 minutes exposure, forced contact)









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

5 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Tunnel test (12 hours, free flying mosquitoes)









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

6 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

WHO test tubes (3 minutes, forced exposure)









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

7 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Test method & efficacy of treated nettings



Deltamethrin 25 mg/m2 Permethrin 500 mg/m2



100 100

90 90

80 80

70 70

% mortality









% mortality

60 Cone 60 Cone

50 Tube 50 Tube

40 Tunnel 40 Tunnel

30 30

20 20

10 10

0 0

0 1 2 3 4 6 8 10 12 0 1 2 3 4 6 8 10 12

Washes Washes









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

8 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New LLINs under WHOPES evaluation



 8 new LLINs under WHOPES testing

– Expected dates for WHO recommendation:

• Dec 06: 1 (BASF Germany)

• June 07: 4 (Tana & Netto, Thailand; Yorkool & Genfont, China)

• Dec 07 : 3 (Clark USA, Syngenta Switzerland, IIC France)

 Current production capacity: 5.3 million/month with

2 producers (63.6 millions/year). Need for planning orders.

 Average FOB price: 4.5 to 5.5 US $/net X-family size (1.6 W x 1.8 L x 1.5 H)



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

9 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Expected production capacity

(based on products already in WHOPES)



25

Million LLINs per month









20



15



10



5



0

Dec-06









Dec-07









Dec-08

Jun-06









Jun-07









Jun-08

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

10 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Costs for LLINs: procurement & distribution



LLINs* Price Delivery cost Programme cost

Mozambique, Olyset (5 years) US$ 5.5 US$ 1.28 US$ 0.68/pp/year

Niger, PermaNet (3 years) US$ 4.16 US$ 1.5 US$ 1

Webster et al., 2006 US$ 5.2 US$ 2.7 (4) US$ 0.8–1.32 (1 – 1.5)



* IFRC Joint LLIN/EPI campaigns, Hoyer 2006, LSHTM





Interesting developments in Africa linking ITN distribution with •

EPI activities (routine and campaigns) & ANC services

Opportunity for achieving full LLIN coverage •

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

11 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

All mosquito nets to be treated



 Treatment of conventional nets (new or in use)

– Development of long-lasting treatment kits (LLT kit)

– 2 LLT kits under WHOPES evaluation (expected Dec 06 Bayer &

Dec 07 Syngenta)



 Achieving full insecticide coverage

– Public sector to purchase LLINs only

– National program to treat existing conventional nets with LLT kits

– Nets locally made or sold through retail market to be bundled

with LLT kits





Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

12 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

African Network for Vector Resistance (ANVR)

Permethrin resistance in An. gambiae s.s.







Kdr mutation East

Kdr mutation West







Kdr frequency in CAR Kdr frequency in Uganda

Data 2000 - 2005

Susceptible

West

Resistance to be confirmed

Resistant East



Highly resistant



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

13 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Selection of resistance, a fast evolving process



 West Africa

– Rapid progression of kdr mutation Northward in An. gambiae M

form (central Côte d'Ivoire: from less 1 % AF in 1995 to more

than 40 % in 2003). Now present in Burkina Faso

– Rapid increase in frequency of kdr in An. arabiensis currently

observed throughout West and East Africa



 Introgression of resistance genes in sibling species

 Dispersal of vectors, population dynamics and gene flow,

are major factors conditioning evolution of insecticide

resistance

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

14 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Need for improved resistance monitoring



 Resistance monitoring to be part of routine operational

activities. Need for:

– strengthening capacity of MNCP to implement monitoring

– national reference laboratory

– specific funding (GFATM, bilateral…)

– regional network for resistance monitoring & management



 Operational impact of resistance to be assessed

 Resistance management tactics (e.g. rotations) to be locally

adapted, especially for residual spraying & larval control

programs

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

15 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Threats to large scale ITN implementation



 Availability of LLINs

 Acceptability

 Mechanisms for rapid scale-up

 Pyrethroid resistance: in case of kdr, no immediate threat to

implementation but concerns in Benin









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

16 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

ITNs: loss of efficacy in Southern Benin*

(experimental huts, An. gambiae)



% MORTALITY ITN: susceptible area 98



ITN: resistant area 30



Untreated net: resistant area 14







% BLOOD-FED ITN: susceptible area 3



ITN: resistant area 82



Untreated net: resistant area 82



0 20 40 60 80 100



* Data M. Rowland, LSHTM

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

17 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

How to address resistance with ITNs?



 Short term response:

– using non-pyrethroids to restore efficacy against resistant

vectors (Ops, carbamates?)

– tentative combination of insecticides on nets to manage

resistance



 Medium term: combine ITNs (as well as IRS) with other

interventions in the context of integrated vector management



 Longer term solution: development of a new insecticide

alternative to pyrethroids





Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

18 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

IRS: loss of efficacy in Southern Benin*

(experimental huts, An. gambiae)



% MORTALITY IRS: susceptible area 69



IRS: resistant area 31



Untreated hut: resistant area 12







% BLOOD-FED IRS: susceptible area 66



IRS: resistant area 73



Untreated hut: resistant area 88



0 20 40 60 80 100



* Data M. Rowland, LSHTM

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

19 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Insecticides for IRS: no miracle expected



 DDT (residual efficacy 6 to 12 months)

– still the cheapest and easiest to use (price, residual efficacy)

– no longer used in agriculture (resistance)

– to be maintained as long as a suitable alternative is available



 Pyrethroids (3 to 6 months)

– six products but equivalent to 1 as far as resistance is concerned

– not an alternative to DDT in kdr areas



 Ops (2 to 3 months) & Carbamates (3 to 6 months)

– share a common resistance mechanism (modified AChE)



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

20 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New products for IRS?



 Longer-lasting formulations (IVCC Gates Foundation)

– microcapsuled Chlorpyrifos-methyl (Reldan)

– bendiocarb resin based formulation (?)



 Already existing contact insecticide: chlorfenapyr (pyrrole)

– different mode of action (no cross resistance with pyrethroids)

– low mammalian toxicity

– on going testing in experimental huts (ITNs & IRS)



 Development of new contact insecticides (NIH, NIAID,

Gates Foundation (Grand Challenge, IVCC)



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

21 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Restoring IRS efficacy:

chlorpyrifos methyl* (experimental huts, An. gambiae)



% MORTALITY Untreated hut 12



DDT 50



lambdacyhalothrin 31



chlorpyrifos methyl 95







% BLOOD-FED Untreated hut 88



DDT 81



lambdacyhalothrin 74



chlorpyrifos methyl 85



0 10 20 30 40 50 60 70 80 90 100



* Data M. Rowland, LSHTM

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

22 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

Larvicides: a range of new products

 Temephos (Abate) to disappear (EU Biocide)



 B. thuringiensis H 14

– Vectobac WDG & tablets for drinking water (dry formulations)

 Pyriproxifen (IGR)

– Sumilarve granules (5 to 10 grams/ha, > 6 weeks efficacy)

– Slow release chips: 20 ppb, > 6 months efficacy in tanks/cisterns

 Spinosins (under WHOPES evaluation)

– Spinosad, 1ppm, 3 months efficacy in closed breeding sites

(alternative to temephos?).



 Novaluron (IGR) (10 to 100 grams/ha), Dinetofuran…and others

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

23 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New approaches: fumigant insecticide



 Metofluthrin (SumiOne, Sumitomo)

– evaporation at room temperature

– efficacy from 1 night to 8 weeks…or more (up to 6 months?)

– safe alternative to coils. Alternative to IRS or ITNs?

– need for field testing efficacy against pyrethroid resistant strains



SumiOne matrix: 8 weeks testing in West Africa (data IRD, Benin)

Tested Mortality Feeding inhibition

Control 1177 1.1

Coil 408 84.6 96.3

Metofluthrin 534 85.6 77.6

Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

24 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New products for specific applications



 Insecticide incorporated

plastic sheeting (ITPS,

80 gsm, HDPE) for refugee

settings and emergencies

– 2 tarpaulins under

development:

• 1 commercial (ZeroFly

deltamethrin, Vestergaard)

• 1 experimental

(permethrin, Sumitomo)

• tents



Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

25 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New products for specific applications





 Insecticide incorporated plastic film

(24 gsm HDPE, 2 % permethrin)

for indoor use (wall and/or roof lining)

– On going testing in Benin (experimental

huts) and Iran (Baluchistan, village trial)

for efficacy, feasibility and acceptability.

Minimum 5 years efficacy targeted.









Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

26 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006

New products for specific applications



 Long lasting insecticide treated curtains

– Protection against domestic pests, malaria, dengue, Chikungunya



 Long-lasting treated blankets for refugee settings

 Wide range of possibilities & opportunities

 Need for program managers to express their needs and

expectations. WHO to stimulate industry to get the right

technology for the right application.





Vector control & new technologies GLOBAL

6th Intercountry meeting of MNCP managers

27 MALARIA PROGRAMME

Cairo, 3 – 6 July 2006


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