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Test Name DHEA

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Test Name: DHEA-S

Specimen Required Serum



Sample Volume (Maximum) 1ml



Sample Volume (Minimum) 0.5 ml



Sample Container Red-top tube/gel-barrier tube



Collection Separate the serum within 2 hours of collection.

Ensure complete clot formation has occurred and there

are no fibrin threads. Do not use lysed serum for testing

as it may give very high results.

Do not use contaminated / turbid samples for testing.

Process the sample on the same day.



Storage Instructions (Stability) 7 days at 2–8 °C

6months@ -20°C



Causes for Rejection Icterus

Hemolysis

Lipemia

Biotin

Presence of fibrin in the sample may lead to erroneous

results and hence centrifuge the sample only after

complete clot formation.

Lysed serum specimens may give falsely elevated

values.

Erroneous findings may be obtained from patients

receiving treatment for monoclonal antibodies or have

received them for diagnostic purposes.



Clinical Significance Used as an aid in the diagnosis of hirsutism and virilism.

In addition to a differential diagnosis of hirsutism and

virilism further indications for this parameter are all

forms of androgenisation, hyperprolactinemia, polycystic

ovarian syndrome and the exclusion of an androgen

producing tumor of the adrenal cortex.



Principle/Method ECLIA method.













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