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Plasmids_KI

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posted:
11/12/2011
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1. Title: p53-responsive EGFP reporter plasmid



Owner: Klas Wiman



Email: Klas.Wiman@cck.ki.se



Partner ID: KI



PI: Klas Wiman



Date: January 5, 2005







p53-responsive EGFP reporter construct carrying 13 p53 DNA binding motifs in front of

a minimal c-fos promoter and the EGFP coding sequence. EGFP was derived from the

plasmid pEGFP-C1 from BD Bioscience. The construct carries the neomycin/kanamycin

resistance gene.









2. Title: GST-wt p53 plasmid-carrying E. coli



Owner: Klas Wiman



Email: Klas.Wiman@cck.ki.se



Partner ID: KI



PI: Klas Wiman



Date: January 5, 2005







E. coli carrying the GST-wt p53 plasmid, The plasmid contains the coding sequence for

glutathione S-transferase (GST) in fusion with the complete coding sequence for wild

type human p53.

3. Title: GST-mutp53 plasmid-carrying E. coli



Owner: Klas Wiman



Email: Klas.Wiman@cck.ki.se



Partner ID: KI



PI: Klas Wiman



Date: January 5, 2005







E. coli carrying the GST-His175 mutp53 plasmid, The plasmid contains the coding

sequence for glutathione S-transferase (GST) in fusion with the complete coding

sequence for His175 mutant human p53.



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