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Office of the Gene Technology Regulator







Risk Assessment and Risk

Management Plan



Application for licence for dealings involving an

intentional release into the environment



DIR 012/2002







Title: Commercial release of Bollgard II® cotton







Applicant: Monsanto Australia Ltd





September 2002

Abbreviations

aad aminoglycoside adenylyltransferase

ANZFA Australia New Zealand Food Authority (now FSANZ)

AQIS Australian Quarantine Inspection Service

Bt Bacillus thuringiensis

B. t.k Bacillus thuringiensis variety kurstaki

CaMv cauliflower mosaic virus

CMoVb caulimovirus figwort mosaic virus

CSD Cotton Seed Distributors Ltd

CSIRO Commonwealth Scientific and Industrial Research Organisation

DIR dealing involving intentional release

DNA deoxyribonucleic acid

DNIR dealing not involving intentional release

ELISA enzyme linked immunosorbent assay

EMBL European Molecular Biology Laboratory

FSANZ Food Standards Australia New Zealand (formerly ANZFA)

EPSPS 5-enolpyruvylshikimate-3-phosphate synthase

GM genetically modified

GMAC Genetic Manipulation Advisory Committee

GMO genetically modified organism

gox glyphosate oxidoreductase

GTTAC Gene Technology Technical Advisory Committee

GUS -glucuronidase

IgE immunoglobulin E

IgG immunoglobulin G

IOGTR Interim Office of the Gene Technology Regulator

IPCS International Program on Chemical Safety

JETACAR Joint Expert Advisory Committee on Antibiotic Resistance

MAFF UK Ministry of Agriculture, Fisheries and Food

MRL maximum residue limit

mRNA messenger ribonucleic acid

NHMRC National Health and Medical Research Council

NICNAS National Industrial Chemicals Notification and Assessment Scheme

NOS nopaline synthase

nptII neomycin phosphotransferase II

NLRD Notifiable Low Risk Dealing

NRA National Registration Authority for Agricultural and Veterinary Chemicals

OGTR Office of the Gene Technology Regulator

ppm parts per million

TGA Therapeutic Goods Administrations

TGAC Technical Grade Active Constituent

US EPA United States Environmental Protection Agency

US FDA United States Food and Drug Administration

WHO World Health Organisation

w/v weight per volume

X-gluc 5-bromo-4-chloro-3-indolyl ß-D-glucuronic acid

μg/g micrograms per gram

TAB LE OF CONTENTS

PREFACE 1



SECTION 1 THE REGULATION OF GENE TECHNOLOGY IN AUSTRALIA ............................................. 1

SECTION 2 THE INITIAL CONSULTATION PROCESSES....................................................................... 1

SECTION 3 THE EVALUATION PROCESS ........................................................................................... 3

SECTION 4 THE STRUCTURE OF THIS DOCUMENT ............................................................................ 5



CHAPTER 1 INTRODUCTION 8



SECTION 1 THE LICENCE APPLICATION ........................................................................................... 8

SECTION 2 SUBMISSIONS ON THE APPLICATION AND RISK ASSESSMENT AND RISK MANAGEMENT

PLAN ............................................................................................................................ 10





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES

13



SECTION 1 THE APPLICATION COMPLIED WITH LEGISLATIVE REQUIREMENTS ............................. 13

SECTION 2 ABOUT THE ORGANISMS TO BE RELEASED .................................................................. 13

SECTION 3 PREVIOUS LIMITED RELEASES OF THESE GMOS IN AUSTRALIA ................................. 14

SECTION 4 RESULTS FROM AUSTRALIAN RELEASES OF BOLLGARD II® AND

BOLLGARD II®/ROUNDUP READY® COTTON ............................................................... 15

SECTION 5 RISK ASSESSMENT AND RISK MANAGEMENT PLANS FOR GENERAL RELEASE OF INGARD®

®

AND ROUNDUP READY COTTON ................................................................................ 17

SECTION 6 APPROVALS FOR GENERAL RELEASE OF INGARD® AND ROUNDUP READY® COTTON AND

ISSUING OF DEEMED LICENCES .................................................................................... 18

SECTION 7 ENVIRONMENTAL MONITORING PROGRAM FOR ROUNDUP READY® AND

ROUNDUP READY®/INGARD® COTTON...................................................................... 20

SECTION 8 APPROVALS FOR INGARD®, ROUNDUP READY® AND BOLLGARD II® COTTON IN OTHER

COUNTRIES .................................................................................................................. 22

SECTION 9 INTERFACE WITH OTHER REGULATORS AND GOVERNMENT BODIES............................ 25

SECTION 9.1 NATIONAL REGISTRATION AUTHORITY FOR AGRICULTURAL AND VETERINARY

CHEMICALS (NRA) .................................................................................................... 25

SECTION 9.2 FOOD STANDARDS AUSTRALIA NEW ZEALAND (FSANZ) ...................................... 26



CHAPTER 3 INFORMATION ABOUT THE GMOS 29



SECTION 1 SUMMARY INFORMATION ABOUT THE GMOS ............................................................. 29

SECTION 2 THE PARENT ORGANISM .............................................................................................. 30

SECTION 3 THE INTRODUCED GENES ............................................................................................. 31

SECTION 3.1 THE CRY1AC GENE ..................................................................................................... 31

SECTION 3.2 THE CRY2AB GENE ..................................................................................................... 32

SECTION 3.3 THE CP4 EPSPS GENE .............................................................................................. 33

SECTION 3.4 THE UIDA REPORTER GENE ....................................................................................... 34

SECTION 3.5 THE NPTII GENE ......................................................................................................... 35

SECTION 3.6 THE AAD GENE ............................................................................................................ 36

SECTION 4 BT TOXINS ................................................................................................................... 36

SECTION 5 METHOD OF GENE TRANSFER ...................................................................................... 39

SECTION 5.2 INGARD® COTTON .................................................................................................... 39

SECTION 5.1 BOLLGARD II® COTTON ............................................................................................. 40

SECTION 5.3 BOLLGARD II®/ROUNDUP READY® COTTON ............................................................ 40

SECTION 6 CHARACTERISATION OF THE INSERTED GENETIC MATERIAL AND STABILITY OF THE

GENETIC MODIFICATION .............................................................................................. 41

SECTION 6.1 BOLLGARD II® COTTON ............................................................................................. 41

SECTION 6.2 BOLLGARD II®/ROUNDUP READY® COTTON ............................................................ 41

SECTION 7 EXPRESSION OF THE INTRODUCED PROTEINS .............................................................. 42

SECTION 7.1 YOUNG LEAVES AND SEEDS ....................................................................................... 43

SECTION 7.2 MATURE COTTON PLANTS ......................................................................................... 44

SECTION 7.3 POLLEN ....................................................................................................................... 45

SECTION 7.4 BOLLGARD II®/ROUNDUP READY® COTTON ............................................................ 49

SECTION 7.3 ROUNDUP READY® COTTON ...................................................................................... 50



CHAPTER 4 RISK ASSESSMENT 51



SECTION 1 THE RISK ANALYSIS FRAMEWORK ............................................................................. 51

SECTION 2 THE RISK ASSESSMENT PROCESS ................................................................................. 51

SECTION 3 SUMMARY OF RISK ASSESSMENT CONCLUSIONS ......................................................... 53

SECTION 3.1 HAZARD IDENTIFICATION ......................................................................................... 53

SECTION 3.2 RISK ASSESSMENT CONCLUSIONS ............................................................................. 54

SECTION 3.3 CONSIDERATION OF RISKS RELATING TO COMBINATION OF THE BOLLGARD II® AND

®

THE ROUNDUP READY TRAITS ................................................................................ 56

SECTION 3.4 IDENTIFICATION OF ISSUES TO BE ADDRESSED FOR COMMERCIAL RELEASE OF

BOLLGARD II® AND BOLLGARD II®/ROUNDUP READY® COTTON .......................... 57

SECTION 4 DECISION TO ISSUE THE LICENCE................................................................................ 58



CHAPTER 5 TOXICITY OR ALLERGENICITY 59







CHAPTER 6 WEEDINESS 85







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 97



SECTION 1 TRANSFER OF INTRODUCED GENES TO OTHER PLANTS ............................................... 97

SECTION 2 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS (MICROORGANISMS AND

ANIMALS) ................................................................................................................... 101





CHAPTER 8 INSECTICIDE RESISTANCE 107

CHAPTER 9 RISK MANAGEMENT PLAN 113



SECTION 1 SUMMARY OF RISK ASSESSMENT CONCLUSIONS ....................................................... 113

SECTION 2 RISK MANAGEMENT PLAN ......................................................................................... 114

SECTION 2.1 RISK OF TOXICITY OR ALLERGENICITY ................................................................. 114

SECTION 2.2 RISKS OF INSECTICIDE RESISTANCE ....................................................................... 114

SECTION 2.3 RISKS OF WEEDINESS OR GENE TRANSFER ............................................................. 115

SECTION 2.4 GENERAL LICENCE CONDITIONS ............................................................................. 116

SECTION 2.5 MONITORING AND ENFORCEMENT OF COMPLIANCE BY THE OGTR ................... 117

SECTION 3 SPECIFIC RISK MANAGEMENT LICENCE CONDITIONS ................................................. 117





CHAPTER 10 CONSIDERATION OF ISSUES RAISED IN PUBLIC SUBMISSIONS 121







REFERENCES 123







APPENDIX 1 SPECIFIC LICENCE CONDITIONS 151







APPENDIX 2 REASONS FOR LICENCE CONDITIONS 171







APPENDIX 3 – PUBLIC SUBMISSION SUMMARY 179

PREFACE



SECTION 1 THE REGULATION OF GENE TECHNOLOGY IN AUSTRALIA



1. The Gene Technology Act 2000 (the Act) took effect on 21 June

2001. The Act, supported by the Gene Technology Regulations 2001,

an inter-governmental agreement and corresponding legislation that is

being enacted in each State and Territory, underpins Australia‘s

nationally consistent regulatory system for gene technology. Its

objective is to protect the health and safety of people, and the

environment, by identifying risks posed by or as a result of gene

technology, and managing those risks by regulating certain dealings

with genetically modified organisms (GMOs). The regulatory system

replaces the former voluntary system overseen by the Genetic

Manipulation Advisory Committee (GMAC).



2. The Act establishes a statutory officer, the Gene Technology

Regulator (the Regulator), to administer the legislation and make

decisions under the legislation. The Regulator is supported by the

Office of the Gene Technology Regulator (OGTR), a Commonwealth

regulatory agency located within the Health and Ageing portfolio.



3. The Act prohibits persons from dealing with GMOs unless the

dealing is exempt, a Notifiable Low Risk Dealing, on the Register of

GMOs, or licensed by the Regulator (see Section 31 of the Act).



4. The requirements under the new legislation for consultation and

for considering and assessing licence applications and preparing risk

assessment and risk management plans are discussed in detail in

Division 4, Part 5 of the Act and Chapter 4 and summarised below.





SECTION 2 THE INITIAL CONSULTATION PROCESSES



5. Under Section 49 of the Act, the Regulator must decide whether

any proposed dealings involving an intentional release into the

environment may pose significant risks to the health and safety of

people or to the environment. If satisfied that at least one of the

dealings proposed to be authorised by a licence may pose a significant

risk, the Regulator must seek written submissions on the application





PREFACE 1

from the public. Regardless of the decision under Section 49, the

Regulator must seek advice on the application from certain prescribed

agencies, and must also seek written submissions from the public and

the prescribed agencies on the risk assessment and risk management

plan prepared in respect of a licence application, in accordance with

Sections 50 and 52 of the Act.



6. In making a decision under section 49 of the Act, section 49(2)

requires that the Regulator must have regard to:

 the properties of the organism to which the dealings relate

before it became, or will become, a GMO;

 the effect, or the expected effect, of genetic modification that

has occurred, or will occur, on the properties of the organism;

 provisions for limiting the dissemination or persistence of the

GMO or its genetic material in the environment;

 the potential for spread or persistence of the GMO or its

genetic material in the environment;

 the extent or scale of the proposed dealings; and

 any likely impacts of the proposed dealings on the health and

safety of people.





7. After taking into account all the matters set out in subsection 49(2),

the Regulator was satisfied that the dealings proposed by these

applications did not pose significant risks to human health, or to the

environment south of latitude 22º South. However, pending the

analysis of additional information supplied by the applicant, she was

unable to determine conclusively from the preliminary assessment

whether or not the proposed releases may pose a significant risk to the

environment north of latitude 22º South. The main reasons for this

were that:

 the insecticidal genes may confer a selective advantage on the

cotton in northern Australia, and this could result in the

GMOs becoming a weed;

 feral cotton populations that outcross with the GMOs may

become a weed.





PREFACE 2

8. Accordingly, the Regulator invited written submissions from the

public on the application under section 49(3) of the Act, on matters

relevant to the preparation of this risk assessment and risk management

plan.



9. As required under Section 50 of the Act, the Regulator also sought

advice on the application to assist the preparation of this risk

assessment and risk management plan from the following prescribed

agencies:

 State and Territory Governments;

 the Gene Technology Technical Advisory Committee

(GTTAC);

 prescribed Commonwealth agencies (Regulation 9 of the Gene

Technology Regulations 2001 (the Regulations) refers);

 the Environment Minister; and

 relevant local council(s).



10. In accordance with Sections 50 and 51 of the Act, the Regulator

has taken account all issues raised in written submissions on the

application in preparing the risk assessment and risk management plan

(see Chapters 1 and 10).



11. All expert groups and key stakeholders, including the public, were

then consulted on the risk assessment and risk management plan in

accordance with Section 52 of the Act.



12. The Regulator has taken into account all issues raised in written

submissions on the risk assessment and risk management plan, relating

to the protection of human health and safety and the environment, in

finalising the risk assessment and risk management plan, and in making

a decision to issue a licence for the proposed release as required under

Section 56 of the Act (see Chapter 9).

SECTION 3 THE EVALUATION PROCESS



13. An assessment of the potential hazards and likely risks associated

with the proposed release was carried out in accordance with the Act,





PREFACE 3

using the Risk Analysis Framework developed by the Regulator (see

Chapter 4, Section 1). A risk assessment and risk management plan,

including proposed licence conditions, was then prepared to address

these risks.



14. In preparing the risk assessment and risk management plan,

information presented by the applicant, the scientific literature,

information from other national regulatory agencies, advice from

scientific experts, as well as submissions from the public and advice

from the Environment Minister, State and Territory Governments,

GTTAC, and Commonwealth agencies (see Regulation 9 of the

Regulations) and local Councils where the release is proposed were

considered and assessed by the Regulator.



15. The legislation requires the Regulator to consider a number of

specific issues in preparing the risk assessment and risk management

plan (see Chapter 4, Section 2). These include: the properties of the

parent organism; the effect of the genetic modification; the potential for

dissemination or persistence of the GMO or its genetic material in the

environment and any provisions for limiting this; the extent or scale of

the proposed dealings; and any likely impacts of the proposed dealings

on the health and safety of people.



16. The legislation also requires the Regulator to consider the potential

of the GMO, in the short and long term, to: be harmful to other

organisms; adversely affect any ecosystems; transfer genetic material to

other organisms; spread or persist in the environment; have a selective

advantage in the environment; and be toxic, allergenic or pathogenic to

other organisms.



17. This document presents the finalised version of the risk assessment

and risk management plan prepared after consideration of all relevant

advice and issues received, in accordance with the legislation, from the

public, interested organisations, States and Territory Governments,

GTTAC, the Environment Minister and the Commonwealth

government agencies, as prescribed by the Act and outlined in

Regulation 9 of the Gene Technology Regulations 2001.



18. Further details about the application can be found in Chapter 3 of

this document. Please note that while copies are available from the





PREFACE 4

OGTR, the application is not available electronically. In the future,

the OGTR hopes that electronic submission of applications will be

possible, enhancing the accessibility of such information for interested

people in the community.





SECTION 4 THE STRUCTURE OF THIS DOCUMENT



19. The document sets out the various matters that were considered by

the Regulator in accordance with Section 51 of the Act in preparing this

risk assessment and risk management plan, and outlines the

consultation processes undertaken under Sections 50 and 52 of the Act.



20. Written submissions sought through these consultation processes

relating to the protection of human health and safety and the

environment have been taken into account by the Regulator in

finalising the risk assessment and risk management plan and were

considered by the Regulator before making a final decision on the

application. This consultation phase was, therefore, an important part

of the decision making process.



21. The structure of the document reflects the matters which the Act

and Regulations require the Regulator to consider in preparing the risk

assessment and risk management plan. This document:

 provides an introduction to the licence application and a

summary of the submissions received on the application and

the risk assessment and risk management plan. Chapter 1

refers.

 summarises the legislative background of the licence

application, and provides background information relating to

previous intentional releases of the GMOs and other related

GMOs. Chapter 2 refers.

 provides detailed information about the parent organism(s),

the GMO(s), and the introduced genes. Chapter 3 refers.

 details the risk assessment undertaken to date in accordance

with the Risk Analysis Framework developed by the

Regulator. Chapter 4 refers.







PREFACE 5

 details the risk assessment on the hazards of toxicity and

allergenicity of Bollgard II® and

Bollgard II®/Roundup Ready® cotton. Chapter 5 refers.

 details the risk assessment on the hazard of weediness of

Bollgard II® and Bollgard II®/Roundup Ready® cotton.

Chapter 6 refers.

 details the risk assessment on the hazard of transfer of

introduced genes to other organisms. Chapter 7 refers.

 details the risk assessment on the hazard of insecticide

resistance. Chapter 8 refers.

 sets out the conclusions reached as a result of the risk

assessment and presents a risk management plan to manage

the identified risks. Conditions included in the licence to give

effect to the risk management plan are also provided.

Chapter 9 refers.

 sets out the specific licence conditions. Appendix 1 refers.

 explains the reasons for specific licence conditions. Appendix

2 refers.

 summarises public submissions on the application.

Chapter 10 and Appendix 3 refer.



22. It should be noted that the OGTR has also produced a reference

document entitled ‗The Biology and Ecology of Cotton

(Gossypium hirsutum L.) in Australia‘(OGTR 2002), which summarises

the current literature and information on cotton, on issues such as

growth and agronomy, outcrossing rates, taxonomy and distribution of

feral cotton and native Australian cotton species and weediness. This

document is referred to throughout the risk assessment and risk

management plan.









PREFACE 6

PREFACE 7

CHAPTER 1 INTRODUCTION



23. The Gene Technology Act 2000 (the Act) and the Gene Technology

Regulations 2001 (the Regulations) set out requirements which the Gene

Technology Regulator (the Regulator) must follow when considering an

application for a licence to intentionally release a genetically modified

organism (GMO) into the environment. Section 51 of the Act requires

the Regulator to prepare a risk assessment and risk management plan

for each licence application. Details of the process which the Regulator

must follow are set out in the Preface.

SECTION 1 THE LICENCE APPLICATION



24. This risk assessment and risk management plan has been prepared

in response to the licence application from Monsanto Australia Ltd

(Monsanto) for the intentional release of genetically modified (GM)

cotton into the environment (DIR 012/2001).



25. Written submissions were sought through the consultation

processes undertaken under Sections 50 and 52 of the Act. These

submissions have been taken into account by the Regulator in finalising

the risk assessment and risk management plan and were considered by

the Regulator before making a final decision on the application.



26. Monsanto applied for a licence to commercially release genetically

modified (GM) insecticidal cotton, registered under the trade name

Bollgard II® cotton, and GM insecticidal/herbicide tolerant cotton,

referred to as Bollgard II®/Roundup Ready® cotton.



27. Bollgard II® cotton is derived from GM insecticidal INGARD®

cotton which contains an insecticidal gene that makes it resistant to the

lepidopteran caterpillar insect pests of cotton. Bollgard II® cotton

contains a second insecticidal gene that increases its insecticidal

activity. Bollgard II®/Roundup Ready® cotton was produced by

conventional breeding of Bollgard II® cotton with GM

Roundup Ready® cotton which contains a gene for tolerance to the

herbicide glyphosate (Roundup®).



28. INGARD® cotton was approved for commercial release in

Australia in 1996, by the National Registration Authority for





CHAPTER 1 INTRODUCTION 8

Agricultural and Veterinary Chemicals (NRA), with advice from the

Genetic Manipulation Advisory Committee (GMAC) which oversaw the

former voluntary system for regulation of gene technology in Australia.

Roundup Ready® cotton was also approved for commercial release in

Australia in 2000, under the voluntary system.



29. These releases were restricted to south of latitude 22 South

because of the concerns about the potential weediness of the cotton in

tropical areas as well as potential for outcrossing to native cotton

species.



30. Monsanto requested approval for the commercial cultivation of

Bollgard II® and Bollgard II®/Roundup Ready® cotton in all Australian

cotton growing areas, including potential cotton growing areas in the

Northern Territory, Western Australia and Queensland, outside the

area previously approved for commercial release of GM cotton.

However, the risk assessment identified a potential risk of weediness in

northern Australia, and the commercial release was not approved for

north of latitude 22º South. The release of GM cotton in the northern

Australia will be restricted to field trials and will be subject to a

number of specific licence conditions to minimise the risk of weediness

by limiting the spread and persistence of the GM cotton in the

environment (see Section 2 of this Chapter and Chapters 6 and 9).



31. Monsanto proposed the phased introduction of the GM

Bollgard II® cotton over three years, associated with the phasing-out of

INGARD® cotton. The total release area for Bollgard II® and

Bollgard II®/Roundup Ready® cotton will be subject to any

requirements that may be imposed by Australian regulatory authorities

for insecticide resistance management (See Chapter 8), but could

eventually encompass a high proportion of the Australian cotton crop

(currently about 484 000 hectares).



32. There have been several limited and controlled releases of

Bollgard II® and Bollgard II®/Roundup Ready® cotton in Australia.

These include trials under the former voluntary system as well as three

licences for limited and controlled releases of Bollgard II® and/or

Bollgard II®/Roundup Ready® cotton issued under the new regulatory

system (see Chapter 2, Section 4).







CHAPTER 1 INTRODUCTION 9

33. It is proposed that the cotton plants and their by-products would

be used in the same manner as conventional cotton, as fibre for the

textile industry as well as in human food and stockfeed. Cotton seed is

processed for oil that is used in a variety of food products. Cotton

linters (a type of short fibre) are used as a cellulose base for several

consumer products, including food.



34. Food Standards Australia New Zealand (FSANZ, formerly the

Australia New Zealand Food Authority, ANZFA), has approved the use

in human food of oil and linters derived from INGARD® cotton and

Roundup Ready® cotton. FSANZ is currently evaluating an

application from Monsanto to approve the use in human food of oil and

linters derived from Bollgard II® cotton. In two previous assessments

of Bollgard II® cotton, FSANZ indicated that it considers products from

Bollgard II® cotton to be as safe for human consumption as those from

conventional cotton (ANZFA 2002).



35. More detailed information about the GMO, the genetic

modification process, the genes that have been introduced and the new

proteins expressed in the GMO is set out in Chapter 3.

SECTION 2 SUBMISSIONS ON THE APPLICATION AND RISK ASSESSMENT AND

RISK MANAGEMENT PLAN



36. Extensive consultation with a range of expert groups and key

stakeholders, including the public, was undertaken in accordance with

sections 50, 51 and 52 of the Act. Issues raised in submissions that

related to public health and safety and the environment were taken into

account in finalising the risk assessment and risk management plan and

the Regulator‘s decision on the application.



37. A summary of the issues follows, along with information on where

they were considered in the risk assessment and risk management plan:

 health concerns including potential toxicity or allergenicity.

These issues are addressed in ‗The Biology and Ecology of

Cotton (Gossypium hirsutum) in Australia‘ (OGTR 2002;

available on the OGTR website) and Chapter 5 of the Risk

Assessment and Risk Management Plan (RARMP);









CHAPTER 1 INTRODUCTION 10

 whether the insecticidal protein in the GM cotton might be

toxic for non-target species. This issue is addressed in

Chapter 5;

 whether the interaction of insecticidal and herbicide-tolerant

traits might be toxic to non-target organisms and soil biota.

This is addressed in Chapter 5.

 whether the GMO may pose a risk as a weed in the

environment, particularly in northern Australia, and whether

herbicide resistant weeds might develop. These issues are

addressed in ‗The Biology and Ecology of Cotton (Gossypium

hirsutum) in Australia‘ (OGTR 2002) and Chapters 6 and 7;

 whether the introduced genes could transfer to other

organisms, including to feral cotton and non-GM cotton crops

and what impacts this might have. These issues are

addressed in ‗The Biology and Ecology of Cotton (Gossypium

hirsutum) in Australia‘ (OGTR 2002) and Chapters 7 and 8



38. Submissions on the risk assessment and risk management plan in

the second round of consultation expressed concerns about river

systems between latitudes 20 and 22 South that can flow towards the

north-west and potentially carry GM material into areas where only

limited and controlled release will be permitted. Based on these

concerns, the risk assessment and risk management plan was revised to

limit the commercial release of the GM cottons to south of latitude

22º South, rather than to 20º South, as proposed in the original risk

assessment and risk management plan.



39. Submissions on the risk assessment and risk management plan

following the second round of consultation confirmed concerns about

the potential weediness of Bollgard II® and

Bollgard II®/Roundup Ready® cotton north of latitude 22º South and

made suggestions regarding their control. Submissions were also

received regarding post-harvest management of the sites including

control of post harvest uses, monitoring of the sites and destruction of

volunteers; transport of GM seed; and whether resistance to the

insecticidal action of the cotton could develop in target pests.









CHAPTER 1 INTRODUCTION 11

40. Submissions also suggested that the following specific issues should

be considered:

 pollen dispersal and buffer zones (see ‗The Biology and

Ecology of Cotton (Gossypium hirsutum) in Australia‘ (OGTR

2002) and Chapters 7 and Chapter 8);

 seed dormancy and seed dispersal (see ‗The Biology and

Ecology of Cotton (Gossypium hirsutum) in Australia‘ (OGTR

2002) and Chapter 6);

 the potential toxicity of the Cry2Ab protein for dipteran

insects (flies and mosquitoes)



41. Accordingly the Licence is subject to a number of specific licence

conditions to minimise the risk of weediness north of latitude 22º South,

by restricting the spread and persistence of the GM cotton in the

environment.



42. Other issues raised in submissions indicated the need for ongoing

monitoring after the release, particularly in relation to the potential for

development of insects resistant to the insecticidal action of the cotton

and herbicide-resistant weeds



43. Submissions from the public are discussed in more detail in

Chapter 10 and Appendix 3.









CHAPTER 1 INTRODUCTION 12

CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS

AND PREVIOUS RELEASES



44. This chapter provides information about the proposed release and

summary information about the GMOs, including information about

previous releases into the environment of relevant GMOs.

SECTION 1 THE APPLICATION COMPLIED WITH LEGISLATIVE REQUIREMENTS



45. The proposal was submitted in accordance with the requirements

of Section 40 of the Act. As required by Schedule 4, Part 2 of the

Regulations, the application included information about:

 the parent organism;

 the GMO;

 the proposed dealing with the GMO;

 interaction between the GMO and the environment;

 risks the GMO may pose to the health and safety of people;

 risk management;

 previous assessments of approvals; and

 the suitability of the applicant.



46. The application also contained:

 additional information required for a GMO that is a plant;

additional information for a GMO that is intended to be used

as food for human or vertebrate animal consumption; and

 supporting information from the Institutional Biosafety

Committee.



47. A copy of the application is available on request from the OGTR.





SECTION 2 ABOUT THE ORGANISMS TO BE RELEASED



48. The organisms to be released are Bollgard II® and

Bollgard II®/Roundup Ready® cotton.









CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 13

49. Bollgard II® cotton, previously known as TWINGARD® cotton,

contains two genes (cry1Ac and cry2Ab) from a soil bacterium, Bacillus

thuringiensis (abbreviated as Bt), that code for insecticidal toxins (Bt

toxins) and make the cotton resistant to lepidopteran caterpillar insect

pests. The cotton also contains bacterial antibiotic resistance genes

(nptII and aad) that were used in the genetic modification process to

select cells containing the desired genes. The nptII gene confers

resistance to kanamycin and neomycin. The aad gene confers

resistance to streptomycin and spectinomycin, but it is not expressed in

plants because the promoter that controls its expression is not active in

plants. A reporter gene (uidA) from Escherichia coli that enables

visual identification of plant tissues in which this gene is being

expressed is also present.



50. Bollgard II®/Roundup Ready® cotton in addition tolerates the

herbicide glyphosate, the active constituent in Roundup® herbicide.

Glyphosate tolerance is conferred by the CP4 EPSPS gene from a soil

bacterium, Agrobacterium strain CP4.



51. Further details about the parent organisms, the genetic

modification process and the introduced genes are provided in

Chapter 3.

SECTION 3 PREVIOUS LIMITED RELEASES OF THESE GMOS IN AUSTRALIA



52. Nineteen limited and controlled releases (field trials) of

Bollgard II® and Bollgard II®/Roundup®Ready have been carried out to

assess the agronomic performance of the GM cotton and its behaviour

in the Australian environment. These releases were carried out under

conditions to minimise potential risks posed by the GM cotton. They

included:

 16 releases approved under the former voluntary system

overseen by GMAC: PR-51X(4), PR-112, PR-112X,

PR-112X(2), PR-118, PR-118X, PR-118X(2), PR-123,

PR-123X, PR-123X(2), PR-131, PR-131X, PR-131X(2),

PR-131X(3), PR-140 and PR-140X; and

 3 releases approved by the Regulator under the new

regulatory system: DIR005/2001, DIR 006/2001, and DIR

009/2001.





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 14

53. The first release of Bollgard II® cotton was in 1999. In the early

releases, Bollgard II® cotton was called TWINGARD® cotton and the

cry2Ab gene was designated as the ‗cryX‘ gene. Each of these releases

was carried out under controlled conditions to limit the spread and

persistence of the GMO in the environment.



54. Bollgard II® and Bollgard II®/Roundup Ready® cotton have been

grown in various Australian locations and conditions, in NSW, QLD,

the NT and WA, to select the best varieties for further development.

Organisations that have been involved in carrying out the releases

include the Western Australia Department of Agriculture, CSIRO and

the Queensland Department of Primary Industries (QDPI), as well as

the cotton seed companies Cotton Seed Distributors Ltd (CSD) and

Deltapine Australia Ltd. In the largest trial, the approved planting

area was 712 hectares.



55. Previous releases conducted under the former voluntary system

were carried out in accordance with GMAC guidelines. To enable

public comment for consideration in the assessment process, each

proposed release was notified in the Gazette, on the GMAC or the

Interim Office of the Gene Technology Regulator (IOGTR) website and

by direct mail to the GMAC or IOGTR mailing list. Relevant local

government councils were also advised directly. Reports were

provided to GMAC or to the OGTR at the conclusion of each release.

No adverse effects on human health and safety or the environment were

reported for any of the previous releases.



56. For releases approved under the regulatory system, consultation

processes were undertaken and risk assessment and risk management

plans prepared in accordance with legislative requirements discussed in

the Preface and Chapter 4. These releases are still in progress, and

reports will be supplied to the OGTR at the end of the release.

SECTION 4 RESULTS FROM AUSTRALIAN RELEASES OF BOLLGARD II® AND

BOLLGARD II®/ROUNDUP READY® COTTON



57. Factors assessed in previous limited and controlled releases (field

trials) of Bollgard II® and Bollgard II®/Roundup Ready® cotton include

agronomic performance, the quality of cotton fibres, possible effects of







CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 15

the Cry1Ac and Cry2Ab toxins on non-target invertebrates, and the

levels of insecticidal activity and herbicide tolerance.



Section 4.1 Agronomic performance



58. Previous field trials in Australia of the Bollgard II® and

Bollgard II®/Roundup Ready® varieties proposed for release have

demonstrated that their growth characteristics, disease resistance, yield

and fibre quality are within the range of current conventional

commercial cotton varieties, and that they have no aberrant

morphological characteristics. Similar studies conducted in the United

States of America (USA) have drawn the same conclusions (data

provided by Monsanto). Effective levels of insecticidal activity and

herbicide tolerance also have been demonstrated under Australian

conditions (Fitt 2000; Greg Constable, Program Leader, Cotton

Improvement and Production, CSIRO Plant Industry, personal

communication).



Section 4.2 Insecticidal activity



59. The insecticidal proteins produced by Bollgard II® cotton target

lepidopteran insects (see Chapter 5), including the major caterpillar

pests of cotton. Studies by CSIRO indicate that Bollgard II® cotton

gives much higher levels of control of the major Helicoverpa caterpillar

species in Australia than INGARD® cotton (Dr G. Fitt, CSIRO

Entomology, CEO Australian Cotton Research Institute, personal

communication). The data suggest that the second protein in

Bollgard II® augments the insecticidal activity of the cotton, relative to

that of INGARD® cotton, by both extending the time over which the

plants express the toxins and by expressing higher levels of toxin (see

Chapter 3, Table1), confirming previous studies undertaken over a

three year period in the USA (Jackson et al. 2000; Akin et al. 2001;

Penn et al. 2001; Stewart et al. 2001; Marchosky et al. 2001).



Section 4.3 Performance of Bollgard II®/Roundup Ready® cotton



60. Field trials in Australia of the Bollgard II®/Roundup Ready®

varieties proposed for release demonstrated that there was no

antagonistic interaction between the insecticidal and herbicide tolerant

traits of Bollgard II®/Roundup Ready® cotton. The level of expression





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 16

of Bt proteins and the insecticidal activity of

Bollgard II®/Roundup Ready® cotton was equivalent to the same indices

of performance of Bollgard II® cotton (data supplied by Monsanto).



Section 4.4 Target range



61. Various researchers have investigated the potential impact of

Bollgard II® cotton on non-target invertebrates, both overseas (Naranjo

and Ellsworth 2002) and in Australia (Addison 2001a; Addison 2001b;

Addison 2001c; see Chapter 5 for details). With the exception of

lepidopteran insects, there were no significant negative effects of

Bollgard II® cotton on the abundance or diversity of non-target

invertebrates.





SECTION 5 RISK ASSESSMENT AND RISK MANAGEMENT PLANS FOR GENERAL

® ®

RELEASE OF INGARD AND ROUNDUP READY COTTON



62. In assessing and establishing the conditions of the initial approvals

for INGARD® (insecticidal) and Roundup Ready® (herbicide tolerant)

cotton, there was extensive co-operation between the NRA, GMAC, the

IOGTR (the OGTR‘s predecessor under the voluntary system),

Commonwealth bodies including Environment Australia, the

Environment Protection Agency and the Australia New Zealand Food

Authority, as well as State and Territory agencies. The NRA and the

IOGTR also undertook public consultation as part of the assessment

process. Responses were received from representatives of consumer,

environmental, farming, cotton industry, and scientific and academic

interests.



63. The potential risks to human health and the environment were

thoroughly assessed by the NRA, GMAC and the IOGTR. They

concluded that risks from both introduced traits to human health were

negligible and that risks to the environment, particularly in the

southern cotton-growing regions of QLD and NSW, were low and could

be managed. The environmental risks identified by GMAC were that

there was a very low risk of outcrossing with native Australian cotton

species, particularly in northern Australia where most of these species

occur. For INGARD® cotton it was considered that there was a low

risk that it might persist as a weed in tropical regions of Australia,





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 17

where naturalised populations of cotton are known to occur, and in

regions where insect resistance might confer a selective advantage (see

Chapter 6, and OGTR 2002).



64. GMAC considered that these risks could be managed by restricting

planting of INGARD® cotton to locations where contact with native

cotton species would be unlikely. GMAC recommended that the

release of both INGARD® and Roundup Ready® cotton be restricted in

location and scale, until further data were available to fully assess the

weediness of the GM cotton, and the likelihood and consequences of

outcrossing with native cotton. The original registration conditions

therefore restricted the general release of the GM cotton to the area

below latitude 26º South and required environmental monitoring to

obtain further information about the potential risks.



65. In 1997, the approved area was extended by the NRA, on the basis

of advice from GMAC, to south of latitude 22º South, as the

cotton-growing industry extended further north in QLD. The

cotton-growing regions of WA and the NT, where the main risks

relating to weediness and outcrossing to native species were considered

to lie, are still excluded.



66. Copies of the licence conditions for INGARD® and

Roundup Ready® cotton are available on the OGTR website. The

licence numbers are GR-3 for INGARD® cotton and GR-9 for

Roundup Ready® cotton. A copy of the risk assessment and risk

management plan for Roundup Ready® cotton (including the risk

assessment and risk management plan for Roundup Ready®/INGARD®

cotton) is also available on the website.

SECTION 6 APPROVALS FOR GENERAL RELEASE OF INGARD® AND

ROUNDUP READY® COTTON AND ISSUING OF DEEMED LICENCES



67. On 5 August 1996, the insecticidal gene present in INGARD®

cotton was registered as an agricultural chemical product by NRA, on

the basis of advice provided by GMAC and other Commonwealth and

State Government Agencies. Commercial planting of INGARD®

cotton initially was limited to 30 000 hectares, but the area has been

extended gradually. The NRA remains responsible for determining the

total planting area each season and for other conditions of registration





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 18

including the implementation of insecticide resistance management

plans (discussed in Chapter 8). In June 2000, the NRA varied the

conditions of registration allowing up to 30% (165 000 hectares) of the

cotton crop to be planted to INGARD® cotton for the 2000-2001 season.



68. Roundup® Ready and Roundup® Ready/INGARD® cotton (GR-9)

were approved for general (commercial) release on 14 September 2000,

by the Minister for Health and Aged Care, the Hon Dr Michael

Wooldridge MP, on the basis of advice from GMAC and the IOGTR.

The Commonwealth Government entered into a legally binding Deed of

Agreement with Monsanto Australia Ltd to ensure compliance with the

agreed conditions. The NRA‘s 30 % cap on INGARD® cotton planting

is still in place and includes Roundup Ready®/INGARD® cotton.

However, no restrictions on the planting area (total hectares) for

Roundup Ready® cotton were imposed. The NRA is responsible for

setting conditions on the new use of Roundup® on cotton crops,

including conditions relating to herbicide resistance management (see

Section 9 of this chapter).



69. Although GMAC concluded from the risk assessment and risk

management plan that led to the approval for general release (Section 5

of this Chapter) that there were no significant risks to the natural

environment arising from the genetic modification of Roundup Ready®

and Roundup Ready®/INGARD® cotton, it recommended that the

release of these GM cottons be reviewed by the OGTR after five years.

Under transitional arrangements set out in Section 190 of the Act,

GMAC‘s advice to proceed with the general release of INGARD®,

Roundup Ready® and Roundup Ready®/INGARD® cotton, issued to

Monsanto, were deemed as licences for the purposes of the Act. The

deemed licences took effect with the commencement of the new

legislation on 21 June 2001. However, the transitional arrangements

under the Gene Technology Act 2000 meant that the approval for

Roundup Ready® and Roundup Ready®/INGARD® cotton could only be

for a maximum of two years after the legislation took effect, on 21 June

2001. As a consequence of these transitional arrangements, the review

of Roundup Ready® cotton is due by June 2003 which is two years

earlier than GMAC‘s recommendation.









CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 19

70. Under the Deed of Agreement, Monsanto was required to conduct

an environmental monitoring program for Roundup Ready® and

Roundup Ready®/INGARD® cotton over three years. This research

program included surveys of:

 the incidence of volunteer Roundup Ready® in

non-agricultural situations (e.g. roadsides);

 the incidence of volunteer Roundup Ready® in non-GM crop

farm situations; and

 any shifts in weed resistance associated with the cultivation of

Roundup Ready® cotton.



71. The progress and the results from this program to date are

summarised below (Section 7 of this chapter).

SECTION 7 ENVIRONMENTAL MONITORING PROGRAM FOR ROUNDUP READY®

® ®

AND ROUNDUP READY /INGARD COTTON



72. The Roundup Ready® and Roundup Ready®/INGARD® cotton

environmental monitoring program was developed through

consultation with GMAC, the cotton industry, scientists, cotton growers

and other interested parties. In addition to the research program

summarised in Section 6 of this Chapter, the consultation established

the following protocols for the management of Roundup Ready® cotton

farming systems:

 integrated weed management guidelines for Australian cotton

production that limit the development of herbicide resistance

in weed species;

 a management plan for controlling Roundup Ready® cotton

volunteers;

 a stewardship program for the Roundup Ready® cotton

technology, comprising a course developed by Monsanto that

each potential grower is required to attend and pass; and

 an annual survey/audit of compliance with the herbicide

resistance management plan that has been ratified by the

herbicide-tolerant subcommittee of the Transgenic and Insect

Management Strategy (TIMS) Committee of the Australian

Cotton Grower‘s Research Association.





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 20

73. The environmental monitoring program for Roundup Ready®

cotton is being conducted by the Australian Cotton Cooperative

Research Centre in the cotton growing area of the Namoi valley, NSW

and Darling Downs in Queensland (Qld). The data gathered to date

were available for this evaluation. The final reports are due by

September 2003. A summary of the results of the research program is

provided in Sections 7.1 – 7.3, below.



Section 7.1 Roundup Ready® cotton in non-agricultural situations

(roadsides)



74. A survey was conducted to determine the incidence of

Roundup Ready® cotton volunteers along roadsides in the lower Namoi

valley. On a total of 700 km of roadside surveyed, an average of 4.3

volunteers/km road were found. However, the volunteers were not

distributed evenly but rather, were clumped in favourable habitats such

as ditches and roadside drains. Evidence from this survey indicates

that control of these volunteers can be managed easily either

mechanically, or with herbicides other than the glyphosate.



Section 7.2 Farm survey for Roundup Ready® cotton volunteers



75. Monsanto provided data for the 2000 - 2001 and 2001 - 2002

seasons relating to surveys of farms in Qld and NSW on which

Roundup Ready® cotton was grown. In the 2000 - 2001 cotton growing

season, about 1,300 hectares of Roundup Ready® cotton were grown

and in the 2001- 2002 season, this increased to 8,600 hectares. The

survey focussed on fields in which Roundup Ready® cotton previously

had been grown, but also included some fields adjacent to the

Roundup Ready® cotton fields. The farm survey showed that 80 – 90

% of the volunteers were Roundup Ready® cotton. In all cases, the

volunteers were controlled easily by cultivation and/or with alternative

herbicides and were not considered to be a problem.



Section 7.3 Survey for shifts in weeds resistant to glyphosate



76. A preliminary report indicates that compliance with an integrated

weed management program and the adoption of Monsanto‘s

stewardship program for Roundup Ready® cotton will significantly

limit the development of weeds resistant to the herbicide, glyphosate.





CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 21

An audit of GM cotton growers indicates that a high proportion of

farmers growing Roundup Ready® cotton comply with Monsanto‘s

technology stewardship program and the herbicide resistance

management plan endorsed by TIMS.



77. The survey of Roundup Ready® cotton volunteers on farms and

roadsides and shifts in the weeds that may develop resistance to

glyphosate is ongoing and will be finalised by September 2003.

SECTION 8 APPROVALS FOR INGARD®, ROUNDUP READY® AND

BOLLGARD II® COTTON IN OTHER COUNTRIES



Section 8.1 Approvals for INGARD® cotton in other countries



78. Countries that have assessed the use of INGARD® cotton include:

 The United States: The US Department of Agriculture and

the Food and Drug Administration approved the commercial

release and use in food of INGARD® cotton in 1995;

 Canada: The Canadian Food Inspection Agency and Health

Canada approved the commercial release and use in food of

INGARD® cotton in 1996;

 Japan: The Japanese Ministries of Agriculture, Forestry and

Fisheries, and Health and Welfare approved the commercial

release and use in food of INGARD® cotton in 1997;

 China: Approved the commercial release and use of

INGARD® cotton in food and feed in 1997;

 Mexico: Approved the commercial release and use of

INGARD® cotton in food and feed in 1997;

 Argentina: Approved the commercial release and use of

INGARD® cotton in food and feed in 1998;

 Indonesia: The National Biosafety Committee approved the

limited commercial release and use in food of INGARD®

cotton in 1999;

 South Africa: The Department of Agriculture approved the

commercial release and use in food of INGARD® cotton in

2000; and







CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 22

 India: Approved the commercial release and use of

INGARD® cotton in food and feed in 2002.



79. Other countries where INGARD® varieties have been approved, or

are pending approval, include Israel, and the European Union.



80. No country has refused an application for the release of INGARD®

cotton.





Section 8.2 Approvals for Roundup Ready® cotton in other countries



81. Countries that have assessed the use of Roundup Ready® and

INGARD® cotton include:

 The United States: The US Department of Agriculture and

the Food and Drug Administration approved the commercial

release and use in food of Roundup Ready® cotton in 1995;



 Canada: The Canadian Food Inspection Agency and Health

Canada approved the commercial release and use in food of

Roundup Ready® cotton in 1996;

 Japan: The Japanese Ministries of Agriculture, Forestry and

Fisheries, and Health and Welfare approved the commercial

release and use in food of Roundup Ready® cotton in 1997.

Its use in animal feed was approved in 1998;

 Argentina: Approved the commercial release of

Roundup Ready® cotton in 1999 and its use in human food

and as stock feed in 2001;



82. In these countries, Roundup Ready®/INGARD® cotton is not

currently regulated separately from the two parental lines. Once the

parental lines were approved for commercial release, the Roundup

Ready®/INGARD® variety was also permitted to be released

commercially.



83. Other countries where both Roundup Ready® varieties have been

approved, or are pending approval, include India, Israel, Mexico, and

the European Union.







CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 23

84. No country has refused an application for the release of Roundup

Ready® cotton.



Section 8.2 Approvals for Bollgard II® cotton in other countries



85. Limited and controlled releases of Bollgard II® cotton have been

approved and carried out in Argentina, Costa Rica, India, Japan,

Mexico, South Africa and the USA. Limited and controlled releases of

Bollgard II®/Roundup Ready®cotton have been approved and carried

out in the USA. An application for limited release of

Bollgard II®/Roundup Ready® cotton in Argentina is under

consideration.



86. Commercial release of Bollgard II® is currently being assessed by

regulators in the USA. Applications seeking approval for the use in

human food of oil and linters from Bollgard II® cotton have been lodged

in the USA and Japan. As noted in Section 1 of this Chapter, FSANZ

(formerly ANZFA) has previously approved the use in human food of

oil and linters from INGARD® and Roundup Ready® cotton and has

indicated that it considers oil and linters from Bollgard II® cotton to be

as safe for human consumption as conventional cotton.



87. No country is known to have refused an application to release

Bollgard II®, Roundup Ready® cotton or Bollgard II®/Roundup Ready®

cotton. No adverse effects on human health and safety or the

environment have been reported in countries in which releases of the

GM cotton have been made.









CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 24

SECTION 9 INTERFACE WITH OTHER REGULATORS AND GOVERNMENT BODIES



88. The OGTR is responsible for assessing the biosafety risks to

human health and the environment associated with development and

use of GMOs. Other government regulatory requirements must also

be met in respect of the release of the GMOs, and the use of products of

the GMO, including the requirements of the NRA and FSANZ.



Section 9.1 National Registration Authority for Agricultural and Veterinary Chemicals

(NRA)



89. Bollgard II® and Bollgard II®/Roundup Ready® cotton fall under

the Agricultural and Veterinary Chemicals Code (1994) definition of an

agricultural chemical product, due to their production of insecticidal

substances, and are therefore subject to regulation by the NRA.



90. As Bollgard II® and Bollgard II®/Roundup Ready® are genetically

modified to resist key insect pests of cotton and, in the case of

Bollgard II®/Roundup Ready® cotton, to tolerate the herbicide

glyphosate, the NRA has imposed conditions in connection with the

insecticidal activity of these cottons to manage the development of

insecticide resistance in the cotton industry as a whole. These are

likely to include specifying maximum areas for release of the GMOs.



91. The NRA has also imposed conditions on the use of the herbicide,

glyphosate (the active ingredient of Roundup®) used in connection with

Roundup Ready® cotton as part of an overall strategy to limit the

development of glyphosate resistant weeds.



92. Therefore, the OGTR has not imposed conditions in relation to

insect resistance management or herbicide tolerance management in

relation to agricultural practices, as these matters will be managed in

the context of the NRA scheme.



93. Further information about the management of insecticide

resistance and on the use and safety of herbicides are available from the

NRA









CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 25

National Registration Authority for Agricultural and Veterinary

Chemicals

PO Box E240

KINGSTON ACT 2604

Phone: (02) 6272 5158

Fax: (02) 6272 4753

Email: nra.contact@nra.gov.au

http://www.nra.gov.au



Section 9.2 Food Standards Australia New Zealand (FSANZ)



94. The safety and labelling of foods derived from genetically modified

plants are the responsibility of FSANZ (formerly ANZFA), rather than

the OGTR.



95. In previous risk assessments of genetically modified INGARD® and

Roundup Ready® cotton, FSANZ found that refined oil and processed

linters derived from these GM cottons are as safe for human

consumption as refined oil and processed linters derived from other

commercial cotton varieties. Similarly, in two previous assessments,

FSANZ has concluded that the oil and linters from Bollgard II® cotton

are as safe for human consumption as those from other conventional

varieties of cotton (ANZFA 2002).



96. Further details of this risk analysis and information about food

labelling are available from ANZFA:



Food Standards Australia New Zealand

PO Box 7186

Canberra Mail Centre ACT 2610

Phone: (02) 6271 2222

Fax: (02) 6271 2278

E-mail: info@foodstandards.gov.au

http://www.foodstandards.gov.au









CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 26

CHAPTER 2 BACKGROUND ON THE APPLICATION, THE GMOS AND PREVIOUS RELEASES 27

CHAPTER 3 INFORMATION ABOUT THE GMOS



97. In preparing the risk assessment and risk management plan, the

Regulator is required under Section 49 (2) of the Act to consider the

properties of the parent organism and the effects of genetic

modification.



98. This part of the document addresses these matters and provides

detailed information about the GMOs for release, the parent organism,

the genetic modification process, the genes that have been introduced

and the new proteins that are expressed in the genetically modified

cotton.



99. Further information and analysis of the properties of the parent

organism are contained in the reference document that was prepared

by the OGTR entitled ‗The Biology and Ecology of Cotton (Gossypium

hirsutum) in Australia‘ (OGTR 2002), available at the OGTR website.





SECTION 1 SUMMARY INFORMATION ABOUT THE GMOS



100. Bollgard II® cotton contains two insecticidal genes, cry1Ac and

cry2Ab, both derived from the common soil bacterium Bacillus

thuringiensis variety kurstaki. The Cry1Ac and Cry2Ab proteins (Bt

toxins) are highly specific insecticidal proteins that are toxic to

lepidopteran caterpillar pests of cotton (Van Rei et al. 1989; Widner &

Whiteley 1989; Van Rie et al. 1990; Dankocsik et al. 1990), including

Helicoverpa armigera (cotton bollworm) and H.punctigera (native

budworm). Further details on the Bt toxins and the cry1Ac and cry2Ab

genes are provided in Sections 3 and 4 of this Chapter.



101. Bollgard II®/Roundup Ready® cotton is also tolerant to the

herbicide glyphosate, the active constituent in Roundup® herbicide.

The herbicide tolerance is conferred by the CP4 EPSPS gene from a soil

bacterium Agrobacterium strain CP4, and was introduced into

Bollgard II® cotton through conventional breeding with GM

herbicide-tolerant Roundup Ready® cotton. Roundup Ready® cotton

was approved for commercial release in 2000 (see Chapter 2, Section 6).









CHAPTER 3 INFORMATION ABOUT THE GMOS 29

102. The modified cotton plants also contain antibiotic resistance genes.

These genes were used as selectable marker genes in the early

laboratory stages of development of the plants, to enable selection of

plant cells or bacteria containing the desired genetic modification.

Potential risks relating to transfer of these genes to bacteria are

discussed in Chapter 7. The antibiotic resistance genes are the

bacterial neomycin phosphotransferaseII (nptII) gene, conferring

resistance to the antibiotics kanamycin and neomycin, and the

aminoglycoside adenylyltransferase (aad) gene. The aad gene confers

spectinomycin and streptomycin resistance and is linked to a bacterial

promoter that does not function in plants, so the protein is not

expressed in Bollgard II® cotton. The antibiotic resistance genes are

discussed in more detail in Section 3 of this Chapter.



103. A gene from Escherichia coli, the uidA gene, which codes for the

bacterial enzyme -glucuronidase (GUS), is also present in the plants.

The GUS protein is a reporter or ‗marker‘ that allows the detection of

genetically modified tissues in the laboratory using a simple

biochemical stain. More information about the uidA gene and GUS

proteins is provided in Section 3 of this Chapter.



104. The cotton lines proposed to be released are backcross progeny of

conventional crosses between Bollgard II® transformation event 15895

and elite Australian cotton cultivars that are suitable for Australian

cotton production areas. In addition, some lines proposed for release

derive from conventional crossing of Bollgard II® cotton and Australian

lines of Roundup Ready® cotton.



105. The methods used to introduce the genes into cotton are discussed

in Section 5 of this Chapter.

SECTION 2 THE PARENT ORGANISM



106. A comprehensive review of the parent organism, Gossypium

hirsutum L. (cultivated cotton), is provided in the document, ‗The

Biology and Ecology of Cotton (Gossypium hirsutum) in Australia‘

(OGTR 2002), available on the OGTR website.









CHAPTER 3 INFORMATION ABOUT THE GMOS 30

SECTION 3 THE INTRODUCED GENES



Section 3.1 The cry1Ac gene



107. The cry1Ac gene in Bollgard II® cotton is in fact a chimeric gene

that combines parts of two genes isolated from Bacillus thuringiensis

variety kurstaki (B.t.k.). Part of the B.t.k cry1Ab gene (nucleotides 1 -

1398, corresponding to amino acids 1 - 466 (Fischhoff et al. 1987)) was

linked to a portion of the native B.t.k cry1Ac gene (nucleotides

1399-3534, corresponding to amino acids 467 - 1178 (Adang et al.

1985)). The cry1Ab region is identical to the analogous region of the

cry1Ac gene with the exception of 6 amino acid substitutions. The

hypervariable region responsible for insecticidal specificity is from the

B.t.k cry1Ac gene. The chimeric gene is therefore referred to in this

document as the cry1Ac gene.



108. Bacterial genes often contain some sequences with potential to act

as polyadenylation sites (often A+T rich), a higher G+C content than is

frequently found in dicotyledonous plant genes, concentrated stretches

of G and C, residues and codons that are not frequently used in

dicotyledonous plant genes.



109. To ensure the bacterial gene was expressed optimally in plants, a

plant-preferred version of the chimeric cry1Ac gene was synthesised

using the strategy described by Perlak et al. (1990; 1991). The amino

acid sequence encoded by the synthetic gene is identical to that of the

native B.t.k protein with the exception that a serine is encoded at

position 766, rather than leucine. This was the result of an

unintentional change that occurred during the synthesis of the

plant-preferred version of the gene. However, the altered amino acid

is not present in the insecticidally-active trypsin-resistant core and will

not change the host range, which is determined by the amino-terminal

portion of the protein (Bietlot et al. 1989). The Cry1Ac protein

expressed in Bollgard II® cotton is 99.4% identical to the B.t.k Cry1Ac

protein (Adang et al. 1985). The properties of the protein are

discussed in Section 4 of this Chapter and Chapter 5.



110. Expression of the cry1Ac gene is driven by a modified 35S

promoter from cauliflower mosaic virus (CaMV; Odell et al. 1985; Kay

et al. 1987). A promoter is a piece of DNA that determines whether or





CHAPTER 3 INFORMATION ABOUT THE GMOS 31

not a gene is expressed, and to what extent. An mRNA termination

region, including a polyadenylation signal, is also required for gene

expression in plants, and is provided by the 3‘ non-translated region of

the soybean alpha subunit of the beta-conglycinin gene (referred to as

the 7S 3‘ termination sequence) (Schuler et al. 1982).



Section 3.2 The cry2Ab gene



111. The cry2Ab gene (also designated as cryIIB, cryB2, P2) in

Bollgard II® cotton was also isolated from B.t.k (Donovan et al. 1988b;

Dankocsik et al. 1990). Very little Cry2Ab protein is produced by

Bacillus thuringiensis due to an inefficient promoter (Dankocsik et al.

1990).



112. There are two very closely related B.t.k cry2Ab genes, cry2Ab1 and

cry2Ab2. They encode identical proteins of 633 amino acids. The

cry2Ab gene in Bollgard II® cotton is the cry2Ab2 gene.



113. The sequence of the cry2Ab gene is 89% identical to another closely

related B.t.k gene, the cry2Aa gene. This represents 88% amino acid

identity (Widner & Whiteley 1989; Dankocsik et al. 1990).



114. A plant preferred version of the cry2Ab gene was constructed as

discussed above for the cry1Ac gene.



115. Expression of the cry2Ab gene is also driven by the modified CaMV

35S promoter (Odell et al. 1985; Kay et al. 1987). This is linked to the

PetHSP70 5‘untranslated leader sequence, from the Petunia hybrida

heat shock protein 70 gene, and the chloroplast transit peptide (CTP)

coding sequence, from the Arabidopsis thaliana EPSPS gene (see below).

The mRNA termination region is provided by the 3‘ non-translated

region of the nopaline synthase (NOS) gene from A. tumefaciens

(Depicker et al. 1982).



116. The chloroplast transit peptide (CTP) coding sequence from the

Arabidopsis thaliana EPSPS gene targets the Cry2Ab protein to the

chloroplast. In plants, EPSPS is synthesised as a preprotein (ie.

containing the CTP) by free cytoplasmic ribosomes. The precursor is

transported into the chloroplast stroma and proteolytically processed to

yield the mature enzyme (della-Cioppa et al. 1986). Once cleaved,

chloroplast transit peptides are rapidly degraded (Bartlett et al. 1982;



CHAPTER 3 INFORMATION ABOUT THE GMOS 32

della-Cioppa et al. 1986). Information provided by Monsanto indicates

that the Cry2Ab protein expressed in Bollgard II® cotton is predicted to

contain three additional amino acids due to processing of the CTP.



117. The Cry2Ab protein expressed in Bollgard II® cotton also contains

a single amino acid addition at the N-terminus, compared to the wild

type amino acid sequence, due to the creation of a restriction enzyme

cleavage site introduced for cloning purposes.



118. The properties of the Cry2Ab protein are discussed in Section 4 of

this Chapter and Chapter 5.



Section 3.3 The CP4 EPSPS gene



119. The gene for CP4 EPSPS, which confers tolerance to glyphosate

(N-(phosphonomethyl) glycine), the active ingredient of Roundup®

herbicide, was isolated from Agrobacterium sp. strain CP4. EPSPS

(5-enolpyruvylshkikimate-3-phosphate synthase) is a critical enzyme in

aromatic amino acid biosynthesis, catalysing the addition of the

enolpyruvyl moiety of phosphoenolpyruvate to shikimate-3-phosphate.

This enzyme is the target of the herbicide glyphosate. Inhibition of

EPSPS by glyphosate prevents the synthesis of chorismate-derived

aromatic amino acids and secondary metabolites (Steinrucken &

Amrhein 1980). CP4 EPSPS naturally resists inhibition by glyphosate

(Padgette et al. 1993).



120. A plant-preferred version of the CP4 EPSPS gene was synthesised

by site-directed mutagenesis (Padgette et al. 1993) and used in the

vector for transformation of cotton plants. Although the gene

sequence has been altered, the protein produced from the

plant-preferred gene has exactly the same sequence as the

Agrobacterium protein. The plant-preferred coding sequence has been

expressed in E. coli from a PRecA-gene 10L vector (Olins et al. 1988).

The proponent states that the activity of the EPSPS protein was

unaltered when compared with the native CP4 EPSPS gene.



121. The CP4 EPSPS gene is driven by the CMoVb promoter (34S

promoter of the caulimovirus figwort mosaic virus) (Richins et al. 1987;

Gowda et al. 1989; Sanger et al. 1990). This is linked to the CTP coding

region from the Arabidopsis thaliana EPSPS gene (Klee et al. 1987). As





CHAPTER 3 INFORMATION ABOUT THE GMOS 33

discussed above for the cry2Ab2 gene, this targets the CP4 EPSPS to the

chloroplast (the site of aromatic amino acid biosynthesis). The mRNA

termination region is from the 3‘ non-translated region of the NOS gene

from Agrobacterium tumefaciens.



122. CP4 EPSPS was detected by Western blot analysis of protein

extracts of Roundup Ready® cotton seed (Barry et al. 1993). An

antibody specific for CP4 EPSPS reacted with a protein of 48 kD. This

is the expected molecular weight for the protein minus the CTP,

confirming that this peptide is cleaved during transport into the

chloroplast.



Section 3.4 The uidA reporter gene



123. The uidA or gusA gene, encoding the enzyme ß-glucuronidase

(GUS), is the most widely used reporter gene in GM plants (Jefferson et

al. 1986; Gilissen et al. 1998). Reporter genes encode enzymes that are

easily assayed and, therefore, are used to ‗report‘ the expression of a

gene or promoter of interest. In this case, it can be used as a simple

biochemical tag to enable visual identification of GM plant tissues.



124. The GUS enzyme cleaves the chromogenic substrate X-gluc

(5-bromo-4-chloro-3-indolyl ß-D-glucuronic acid), resulting in the

production of an insoluble blue colour in those plant cells expressing

GUS activity. Non-GM plant cells do not in general contain any GUS

activity, although there are unconfirmed reports of a GUS-like activity

in some plant tissues. Therefore, the production of a blue colour in a

particular cell after staining with X-gluc indicates that the cell has been

successfully genetically modified to contain the uidA gene. Thus the

uidA gene serves as a ‗marker‘ gene, since its expression marks tissue in

which other co-introduced genes are also present and likely to be

expressed (Jefferson et al. 1987; Jefferson & Wilson 1991).



125. Particle bombardment is not particularly efficient at introducing

genes into plants, and screening for the uidA reporter gene facilitates

identification and recovery of plant material containing the genetic

modification. Tight linkage between the uidA gene and the cry2Ab

genes (see Section 6.1 of this Chapter) has the additional advantage that

GUS staining can be used to follow the segregation of Bollgard II®







CHAPTER 3 INFORMATION ABOUT THE GMOS 34

cotton‘s two insecticidal genes in segregating populations in backcross

breeding programs.



126. The uidA gene is from the bacterium Escherichia coli. This

bacterium has evolved to survive in the mammalian intestine, and the

enzyme encoded by the uidA gene enables it to utilise as its sole carbon

source -glucuronides excreted in mammalian guts as by-products of

the detoxification of compounds including certain antibiotics and

hormones.



127. Escherichia coli GUS protein, a monomer with a molecular weight

of about 68200 daltons, is probably active in a tetrameric form. GUS is

an exo-hydrolase; it will not cleave glucuronides in internal positions

within polymers. The enzyme is specific for -D-glucuronides, with

some tolerance for -galacturonides. It is inactive against -glucosides,

-galactosides, -mannosides, or glycosides in the alpha configuration.



128. Expression of the uidA gene in Bollgard II® cotton is controlled by

the CaMV 35S promoter (Odell et al. 1985; Kay et al. 1987). The

mRNA termination region is provided by the 3‘non-translated region of

the NOS gene from A.tumefaciens (Depicker et al. 1982).



129. Monsanto has recently provided DNA sequence analysis of the

uidA gene in Bollgard II®. These analyses demonstrate that the

plant-expressed version of the uidA gene encodes a single amino acid

substitution at position 377 in the GUS protein, relative to the GUS

protein produced in E.coli. This substitution predicts the replacement

of a glutamic acid (E) with a lysine (K) at this position. Modelling

studies and amino acid and protein structure comparisons of

-glucuronidases from a variety of sources indicate that the amino acid

substitution is not likely to confer any significant structural or

functional changes in the GUS protein produced in Bollgard II® cotton.

The amino acid substitution was present in the original Bollgard II®

cotton varieties used to obtain the experimental data provided to the

OGTR and other regulatory agencies for safety assessments.



Section 3.5 The nptII gene



130. The nptII gene was isolated from the bacterial Tn5 transposon

(Beck et al. 1982). It encodes the enzyme neomycin





CHAPTER 3 INFORMATION ABOUT THE GMOS 35

phosphotransferase type II (NPTII) which confers resistance to

aminoglycoside antibiotics such as kanamycin and neomycin.



131. The NPTII enzyme uses ATP to phosphorylate neomycin, and the

related kanamycin, thereby inactivating the antibiotic and preventing it

from killing the NPTII producing cell. The nptII gene functions as a

selectable marker in the initial laboratory stages of cotton plant cell

selection following transformation (Horsch et al. 1984; De Block et al.

1984), allowing transformed cells to grow while effectively inhibiting

the growth of non-transformed cells. It is also expressed in the

Bollgard II® and Bollgard II®/Roundup Ready® cotton plants.



132. The gene is controlled by the CaMV 35S promoter (Odell et al.

1985; Kay et al. 1987). The mRNA termination region of the gene is

from the 3‘ non-translated region of the NOS gene from A. tumefaciens

(Rogers et al. 1985).



Section 3.6 The aad gene



133. The aad gene was isolated from the bacterial Tn7 transposon and is

under the control of its own bacterial promoter. This gene encodes an

enzyme, 3‘(9)-O-aminoglycoside adenylyltransferase (AAD), which

adenylates either the 3‘-hydroxy on the amino-hexose III ring of

streptomycin or the 9-hydroxyl on the actinamine ring of spectinomycin

(Davies & Benveniste 1974), allowing growth of bacteria on medium

containing these antibiotics. The gene was used in the laboratory,

prior to the production of the genetically modified plants, to select for

bacteria containing the desired modified DNA. The gene is not

expressed in the Bollgard II® or Bollgard II®/Roundup Ready® cotton

because the bacterial promoter is not active in plants. The nucleotide

sequence of this gene in the Tn7 transposon has been determined (Fling

et al. 1985).

SECTION 4 BT TOXINS



134. Bacillus thuringiensis is a gram-positive bacterium found in soil

and other habitats throughout the world. During sporulation, B.

thuringiensis produces a parasporal crystal composed of proteins

known as -endotoxins or insecticidal crystal proteins. The crystal

proteins are often specifically toxic to certain insect species.





CHAPTER 3 INFORMATION ABOUT THE GMOS 36

135. Cry1Ac and Cry2Ab are two of a diverse family of insecticidal

proteins (Bt proteins or Bt toxins) found in the bacterium Bacillus

thuringiensis. The Bt proteins have been classified depending on the

target specificity and the degree of amino acid homology (Hofte &

Whiteley 1989). The current classification is defined by the latter

criterion (Crickmore et al. 1998).



136. Cry1Ac toxins are highly specific to lepidopteran insects (moths

and butterflies) (Widner & Whiteley 1989; Dankocsik et al. 1990;

Macintosh et al. 1990). Cry2A toxins are also specific to lepidoptera,

but certain subclasses of Cry2A proteins may also be toxic to particular

groups of Diptera (flies and mosquitoes; Sims 1997) (see Chapter 5).



137. The Cry2Ab proteins were previously referred to as CryX,

Cry2Ab1, Cry2Ab2, CryIIAb, CryIIB or CryB2 (Widner & Whiteley

1989; Widner & Whiteley 1990; Liang & Dean 1994; Crickmore et al.

1998).



138. The native crystal proteins found in B. thuringiensis are protoxins

that must be digested by the insect to become activated. In susceptible

insects, during ingestion, the Bt protein crystal dissolves in the alkaline

environment of the larval insect gut. In many cases, activation of the

toxin by cleavage with specific proteases in the gut is required,

particularly for the larger protoxins, such as Cry1Ac, of 130-140 kDa

(Gill et al. 1992). Proteases cleave the carboxy-terminal domain of the

Cry1Ac protein and approximately 28 amino acids from the

amino-terminal end of the protein, leaving an active protease-resistant

core of approximately 600 amino acids (Bietlot et al. 1989; Chroma &

Kaplan 1990).



139. The Cry2 proteins are smaller than the other Cry protein classes

(Cry2Aa and Cry2Ab are both 633 amino acids) and may not require

protease cleavage for activation (Gill et al. 1992; English et al. 1994;

Karim et al. 2000).



140. The active Bt toxins diffuse through the midgut membrane of the

target lepidopteran insects and bind to specific high affinity receptors

on the midgut epithelium surface (Hofmann et al. 1988; Van Rei et al.

1989; Van Rie et al. 1990; Karim et al. 2000). Non-target insects,







CHAPTER 3 INFORMATION ABOUT THE GMOS 37

mammals, birds and fish do not possess these receptors and therefore

are not susceptible to the toxic effects of these insecticidal proteins.



141. Binding of activated Bt toxins to the epithelial gut cell receptors

leads to formation of pores in the cell membrane, allowing leakage of

intracellular contents (for example potassium ions) into the gut lumen

and water into the cell (Sacchi et al. 1986; English & Slatin 1992;

Knowles & Dow 1993). The larval gut epithelial cells swell due to

osmotic pressure and lyse. The gut becomes paralysed because of

changes in the electrolyte and pH balance and the insects stop eating

and die (Goldburg et al. 1990).



142. Competition studies indicate that Cry1Ac and Cry2Aa bind to

different receptors in target insects (Morse et al. 2001). The mode of

action of Cry2A has also been studied and compared to that of Cry1Ac

(English et al. 1994). The two proteins exhibit differences in solubility,

binding capacity and ion channel formation. Unlike Cry1Ac, Cry2A

did not exibit saturable binding on the midgut brush border membrane

of Helicoverpa zea. Cry2A did not block Cry1Ac binding but Cry1Ac

did block the initial binding of Cry2A to the brush border, suggesting

that some component of binding must be shared between the two toxins.

Although Cry2A was found to be less soluble than Cry1Ac, Cry2A

arrested the feeding of third instar H. zea as rapidly as did Cry1Ac.

Larvae fed on Cry1Ac tended to regurgitate more rapidly than those

fed on Cry2A. Insects fed with Cry2A were not seen to recover from

the intoxication, as did some insects fed with Cry1Ac, when removed

from the toxin-laced diet. It was suggested that the differences in the

mode of action between the two toxins made Cry2A a more rapidly

toxic agent.



143. The Cry1Ac protein expressed in INGARD® cotton was compared

by Western blot analysis with commercially available microbial

pesticides containing Bt toxin (Berberich & Fuchs 1992). This study

showed that the protein expressed by INGARD® cotton is of similar

molecular weight and immunological reactivity to one or more proteins

contained in the commercial Bt products Dipel® (Abbott Laboratories)

and Thuricide® (Sandoz Inc.). Further, it has been demonstrated that

the biological activity and species-specificity of the full-length Cry1Ac







CHAPTER 3 INFORMATION ABOUT THE GMOS 38

protoxin expressed in INGARD® cotton is equivalent to that of the

active B.t.k Cry1Ac core toxin (Sims 1994a).

SECTION 5 METHOD OF GENE TRANSFER



Section 5.2 INGARD® cotton



144. Bollgard II® cotton is derived from INGARD® cotton (known as

Bollgard® cotton in the USA). INGARD® cotton contains the cry1Ac,

nptII and aad genes, originally inserted into the genomic DNA of the

Coker 312 cotton variety (transformation event 531). Coker 312 was

released in 1974 by the Coker Pedigree Seed company. The Coker 312

cultivar was used because of its positive response to the tissue culture

system used to produce transgenic plants. However, it is now grown

commercially on a very limited basis, if at all.



145. The genes were inserted by Agrobacterium-mediated DNA

transformation (Zambryski 1992). The plasmid vector that was used,

PV-GHBK04, is a binary, single-border transformation vector. The

plasmid contains well-characterised DNA segments required for

selection and replication of the plasmid in bacteria as well as

Agrobacterium sequences. The above characteristics are essential for

DNA transfer from Agrobacterium and integration in the plant cell

genome (Bevan 1984; Wang et al. 1984)



146. Agrobacterium tumefaciens is a common gram-negative soil

bacterium that causes crown gall disease in a wide variety of plants.

Plants can be genetically transformed by the transfer of DNA (T-DNA,

located between specific border sequences) from A. tumefaciens,

through the mediation of genes from the vir (virulence)7 region of Ti

plasmids. Disarmed Agrobacterium strains have been constructed

specifically for plant transformation. The disarmed strains do not

contain the genes (iaaM, iaaH and ipt) responsible for the

overproduction of auxin and cytokinin, which are required for tumour

induction and rapid callus growth (Klee & Rogers 1989). A useful

feature of the Ti plasmid is the flexibility of the vir region to act in

either cis or trans configurations to the T-DNA. This has allowed the

development of two types of transformation systems:









CHAPTER 3 INFORMATION ABOUT THE GMOS 39

 co-integration vectors that join the T-DNA that is to be

inserted into the plant and the vir region in a single plasmid

(Stachel & Nester 1986); and

 binary vectors that have the T-DNA and vir regions

segregated on two plasmids (Bevan 1984).



147. Both provide functionally equivalent transformation systems.

Agrobacterium-mediated transformation has been widely used in

Australia and overseas for introducing new genes into plants without

causing any biosafety problems.



Section 5.1 Bollgard II® cotton



148. Bollgard II® cotton was produced by inserting the cry2Ab and uidA

genes into the genomic DNA of INGARD® cotton variety DP50B.

Isolated DNA fragments were delivered into the cotton meristematic

cells by projectile bombardment (transformation event15985) (McCabe

& Martinell 1993).



149. Projectile bombardment is a physical delivery system whereby

minute gold or tungsten beads coated with DNA are shot into cells that

have the capacity to develop or differentiate into shoots or whole plants.

The uidA gene is used as a marker to identify plant tissue that is

containing the introduced gene of interest so that the transformed tissue

or seed can be selected and recovered.



Section 5.3 Bollgard II®/Roundup Ready® cotton



150. Bollgard II®/Roundup Ready® cotton was produced by

conventional cross breeding of Bollgard II® cotton and

Roundup Ready® cotton. Roundup Ready® cotton was produced by

inserting the CP4 EPSPS, nptII and aad genes on a plasmid vector

carried by disarmed strain of A.tumefaciens into the genomic DNA of

cotton variety Coker 312. The method used to insert the genes was the

same as that described above for generating INGARD® cotton, that is

via Agrobacterium-mediated transfer, using a binary, single-border

transformation vector, plasmid vector PV-GHGT07 (Bevan 1984;

Wang et al. 1984).









CHAPTER 3 INFORMATION ABOUT THE GMOS 40

SECTION 6 CHARACTERISATION OF THE INSERTED GENETIC MATERIAL AND

STABILITY OF THE GENETIC MODIFICATION



Section 6.1 Bollgard II® cotton



151. Southern blot analysis of Bollgard II® DNA shows that one

full-length copy of each of the cry2Ab and uidA (GUS) genes is present

(Doherty et al. 2000a; Doherty et al. 2000b). Recent PCR and sequence

analyses provided by Monsanto demonstrate the linkage of elements

contained within the insert that contains the cry2Ab and uidA genes

(Pineda et al. 2002). The cry1Ac and cry2Ab genes have been

maintained as single dominant Mendelian traits over many generations

of crossing and backcrossing, with no significant variation from

expected segregation ratios for the two insecticidal genes (Shappley

2002b). These data demonstrate that cry1Ac and cry2Ab segregate

independently of one another, and have therefore as expected been

inserted at regions of the plant genome distant from each other.



152. The stability of the DNA insert and expression of the Cry2Ab

protein in Bollgard II® cotton across five generations has been

confirmed by data from Southern blot, ELISA and Western blot

analysis (Bookout et al. 2001; Pineda et al. 2002).



Section 6.2 Bollgard II®/Roundup Ready® cotton



153. As noted above, Bollgard II®/Roundup Ready® was produced by

conventional breeding of Bollgard II® cotton and Roundup Ready®

cotton. The genes introduced into Roundup Ready® cotton are also

present, therefore, in Bollgard II®/Roundup Ready® cotton.



154. Southern blot analysis was used to demonstrate that a single copy

of the nptII, CP4 EPSPS and aad genes has been inserted in

Roundup Ready® cotton. The insert was maintained in a stable

condition within the cotton genome for three generations (data

supplied by Monsanto Australia Ltd). The gox gene, from the

bacterium Ochromobacterium anthropii (encoding the glyphosate

oxidoreductase enzyme (GOX)), although present in the intermediate

plasmid vector, was not transferred to the plant genome. The T-DNA

region of the insert was truncated before the gox gene began (this is not







CHAPTER 3 INFORMATION ABOUT THE GMOS 41

uncommon, see for example Bakkeren et al. (1989) and De Block et al.

(1984)).



155. No data have been presented for Bollgard II®/Roundup Ready®

cotton in relation to the stability of the genetic modifications.

However, Monsanto have supplied data from analyses of

INGARD®/Roundup Ready® cotton demonstrating that segregation

ratios for the cry1Ac and CP4 EPSPS genes in

INGARD®/Roundup Ready® cotton do not differ significantly from

those that are expected based on Mendelian genetics (Shappley 2002a).

The data also demonstrate that the cry1Ac and CP4 EPSPS genes are

stably integrated at separate loci in the genome of

INGARD®/Roundup Ready® cotton (Shappley 2002a).



156. The combination of Bollgard II® and Roundup Ready® traits in the

one GMO was achieved by conventional breeding, and the stability of

the genetic modifications in Bollgard II®/Roundup Ready® cotton can

be inferred from the stability of Bollgard II® and Roundup Ready®

cotton over several generations. This is also supported by the stability

and segregation data presented for INGARD®/Roundup Ready® cotton.



157. More direct evidence for the stability of the genetic modifications

in the Bollgard II®/Roundup Ready® cotton comes from glasshouse and

field studies that have demonstrated continued efficacy of the

Bollgard II® insecticidal and Roundup Ready® herbicide tolerance

traits, in releases carried out since 1999 (Dr D.Llewellyn, Senior

Principal Research Scientist, CSIRO Plant Industry, personal

communication).

SECTION 7 EXPRESSION OF THE INTRODUCED PROTEINS



158. Expression levels of Cry2Ab, Cry1Ac, GUS and AAD proteins

were estimated in samples Bollgard II®, INGARD® and conventional

cotton. Samples of young leaves, whole plants, seed and pollen were

collected and analysed using an enzyme-linked immunosorbent assay

(ELISA) (information provided by Monsanto Australia Ltd). Tissue

samples were collected from plants grown in eight of the major cotton

production regions of the USA and comprised regions representing a

variety of environmental conditions. Samples were collected in both

the 1998 and 1999 cotton-growing seasons.





CHAPTER 3 INFORMATION ABOUT THE GMOS 42

159. Additionally, variation in the expression of Cry2Ab was examined

in young leaves sampled every four weeks from two sites, to investigate

variation in the level of protein expression within the growing season.

Such seasonal variation was also examined in both 1998 and 1999.



Section 7.1 Young leaves and seeds



160. Mean expression of Cry2Ab in young leaves was about 6g/gand

24g/gof tissue (equivalent to ppm, i.e. parts per million), in 1998 and

1999, respectively (Table 1), and was consistent across all sampled

regions. Variation in protein levels between years probably reflects

differing environmental conditions, notably extreme rainfall in the 1998

American cotton-growing season (data provided by Monsanto). As the

levels of protein are measured per fresh weight of tissue, it is likely that

variation in plant moisture between years contributed to the observed

differences. Significantly higher rainfall during the 1998 season would

have ensured that plants received comparatively more moisture and

that the leaves would have been more turgid. In 1998, the amount of

protein as a proportion of fresh leaf weight would, therefore, have been

comparatively low.



161. Within the cotton-growing season, expression of Cry2Ab protein in

young leaves peaked at 55 and 85 days after planting in 1998 (40g/g)

and 1999 (17g/g), respectively. Expression continued at high levels

for at least 108 days (Table 2). As noted, differing rainfall between

years probably accounts for the variation in peak expression levels. As

expected, Cry2Ab protein was undetectable in young leaves of both

INGARD® and conventional cotton (Table1).



162. Cry2Ab protein levels in seeds of Bollgard II® cotton were higher

than in young leaves — about 43g/g in 1998 and 57g/g in 1999,

respectively (Table 1). Again, Cry2Ab was undetectable in the seeds of

either the INGARD® or conventional cotton controls for this protein.



163. Mean expression levels of Cry1Ac were consistently lower than

those of Cry2Ab in all sampled tissues of both Bollgard II® and

INGARD® cotton. Expression of Cry1Ac in Bollgard II® was similar to

that in INGARD® in both 1998 and 1999 (Table 1). The results

indicated that the expression of Cry1Ac proteins is not altered by the

additional genetic modification. Independent data also indicate that





CHAPTER 3 INFORMATION ABOUT THE GMOS 43

expression of Cry2Ab in Bollgard II® cotton has no significant effect on

expression of Cry1Ac, compared to the levels of Cry1Ac in INGARD®

cotton (Greenplate et al. 2000).



164. In 1998 levels of Cry1Ac in young leaves and seeds (≤ 3.35g/g)

were marginally higher than in 1999 (≤ 2.64g/g). In contrast to the

within-season levels of Cry2Ab, levels of Cry1Ac declined significantly

to negligible levels at the end of the season, after peaking about

half-way through the season in both 1998 and 1999 (Table 2).



165. In a separate study, Cry1Ac levels in the fruit and terminals of

transgenic cotton have also been reported to decline steadily as the

growing season progresses (Greenplate 1999). Cry1Ac protein levels in

the fruiting positions dropped from 10-15 g/g at 40 days after planting

to 1-2 g/g at 120 days. Similarly, Cry1Ac levels in terminal foliage

declined from approximately 20 g/g to 5g/g between 40 and 120 days

after planting.



166. Expression of NPTII protein in Bollgard II® cotton was similar to

that in INGARD® cotton (ca. 4.6 - 16.6 g/g and 4.3 – 15.2 g/g, for

Bollgard II® and INGARD® cotton, respectively; Table 1). The results

indicated that as for Cry1Ac, expression of the NPTII protein in seed

and leaf tissues is unaffected by the insertion of the cry2Ab gene.



167. GUS protein was detected at high levels in both young leaves (≤

120g/g) and seeds (≤ 104g/g) in 1998 and 1999, respectively (Table 1).

As expected, GUS could not be detected in either the INGARD® or

conventional cotton controls for this assay.



168. AAD protein was not detected in the leaf or seed tissue of any of

the cotton lines that were assayed. This outcome was expected since

the gene is under the control of a bacterial promoter that is not active in

plants.



Section 7.2 Mature cotton plants



169. The level of Cry2Ab in mature Bollgard II® plants was generally

lower than in leaf and seed tissue, with averages of 8.8g/g and 20.8g/g

in 1998 and 1999, respectively (Table 1). Cry2Ab was not detected in

whole plant samples of the control lines, as expected.





CHAPTER 3 INFORMATION ABOUT THE GMOS 44

170. Within a season, Cry1Ac was detected at similar levels in both

Bollgard II® and INGARD® cotton (Table 1). Seasonal variation in

expression of Cry1Ac probably reflects different environmental

conditions, as outlined above for variation in expression of Cry2Ab. In

Bollgard II® cotton, levels of Cry1Ac were much lower than levels of

Cry2Ab (Table 1). GUS and NPTII protein levels were not analysed in

whole cotton plants.



Section 7.3 Pollen



171. To provide information on the potential for non-target insect

exposure to the toxin, the levels of Cry proteins in pollen were

estimated. In both 1998 and 1999, negligible amounts (≤ 0.32 g/g) of

protein encoded by the inserted genes were detected in pollen from both

Bollgard II® and INGARD® cotton (Table1).



172. Quantitative bioassays (Greenplate 1999) with Heliothis virescens

incorporating plant tissues in synthetic diets compared to known

concentrations of purified Cry1Ac, were used to evaluate the overall

insecticidal activity of different tissues to lepidoptera. The mean

insecticidal activity was expressed as g Cry1Ac equivalents/g dry

weight (Penn et al. 2001). In Bollgard II® plants this represents the

combined insecticidal activity of the Cry1Ac and Cry2Ab proteins. In

INGARD® cotton insecticidal activity was highest in growing tips (24 g

Cry1Ac equivalents/g tissue), slightly lower in flower buds (20 g

Cry1Ac equivalents/g tissue) and lower still in large leaves (18 g

Cry1Acequivalents/g tissue). Bollgard II® had consistently higher

mean lepidopteran activity with 81, 90 and 50 g Cry1Ac equivalents/g

dry weight in growing tips, flower buds and large leaves, respectively.



173. Averaged over all sites, tissues and seasons Bollgard II® cotton was

3.9 times more effective in controlling H. virescens than the

corresponding INGARD® cotton line. The relatively high expression

level of the Cry2Ab compensates for its lower insecticidal activity

against many of the target insect pests. These data support the

observed higher efficacy of insect control of Bollgard II® towards a

range of important lepidopteran pests (Akin et al. 2001; Stewart et al.

2001).







CHAPTER 3 INFORMATION ABOUT THE GMOS 45

174. The bioassays described above were also used to measure the mean

insecticidal activity of small flower buds from Bollgard II® and

INGARD® cotton over an eight-week period following planting (Penn et

al. 2001). The insecticidal activity of Bollgard II® cotton dropped from

107 g Cry1Ac equivalents/g tissue at 2 weeks to 52g Cry1Ac

equivalents/g tissue by eight weeks, but was still 2.9 fold higher than the

single gene INGARD® cotton that had declined from 25 to 18 g

Cry1Ac equivalents/g tissue in the same period. Similar observations

have been reported by (Stewart et al. 2001) and highlight the relatively

low efficacy of INGARD® cotton at the end of the growing season.

Further, they suggest that Bollgard II® cotton should have

comparatively high insecticidal efficiency late in the growing season.









CHAPTER 3 INFORMATION ABOUT THE GMOS 46

Table 1. Mean protein expression levels* in various tissues of Bollgard II® (event 15985), INGARD® (line DP50B)

and conventional cotton (line DP50), as detected by ELISA, ± standard deviation.



Protein Cotton type Leaf Seed Whole plant Pollen

1998 1999 1998 1999 1998 1999 1998 1999

®

Cry2A Bollgard II 23.8 ± 6.3 6.4 ± 1.443.2 ± 5.7 57.4 ± 8.8 ± 1.2 20.8 ± 300. Six of these sites were particularly productive,

producing a total of ≥20 bolls, with estimates suggesting that individual plants

produced comparable numbers of bolls to those produced by cultivated cotton

plants. Significantly, these were disturbed sites (drain and cattleyard

habitats), with high levels of soil nutrients and/or moisture. A sample of 3

bolls produced by INGARD® cotton at one of the less productive sites

suggested that both seed production and seed viability was low. These

characteristics of seeds produced during the experiment were not investigated

in either Bollgard II® or conventional cotton at any of the other sites. They

are likely to vary by site, associated with variation in the maternal

environment (Mazer & Gorchov 1996).



318. Formal analyses of total site fruit production was not possible for all sites

because of low numbers. However, Monsanto reported detailed statistical

analyses for three of the productive disturbed sites. At two of these,

Bollgard II® cotton produced significantly more fruit than conventional

cotton by the end of the first season, although for one of the sites, the impact

was statistically significant (P < 0.01) only in plots that were sown with seeds

at high density. After two years, the advantage apparently conferred by the

insecticidal genes remained significant at only one of the two sites. The

analyses have not yet been finalised, but it appears that the productivity of

Bollgard II® cotton may have been marginally lower than that of conventional

cotton at the second site, in the second year. The third productive site was

established one year after the other sites, so boll production was scored for 12

months only. At the end of the growing season boll production did not differ

significantly between Bollgard II® and conventional cotton at this third site.







CHAPTER 6 WEEDINESS

319. Preliminary analyses of experiments to test whether enhanced nutrient

availability is responsible for the increased boll production suggest that this

may not be the case (Rowena Eastick, personal communication). The results

of these experiments have not yet been provided to the OGTR for evaluation.

In their absence, it is concluded that these potentially productive sites, which

are most likely to be in areas immediately associated with agricultural

production rather than in the natural environment, the risk of Bollgard II®

cotton establishing could be managed relatively easily by monitoring for, and

removing, potential volunteers.



Invasiveness



320. To provide a broad indication of potential invasiveness, Monsanto

compared net population growth rate (‗invasiveness‘) of Bollgard II®,

INGARD® and conventional cotton at each study site. Calculating this index

requires considering any deaths that may occur, as well as any new recruits

into the population. The term therefore integrates elements of germination,

survival, and reproduction (fecundity), as detailed above. Invasiveness

values greater than one imply that the population is growing, and that it will

continue to grow until some resource(s) becomes limiting, as occurs under

normal environmental conditions. Invasiveness values less than one imply

that the population is in decline. The function is therefore similar to the

population growth rate function (λ) discussed by several authors (see Crawley

et al. 1993; Parker & Kareiva 1996). Generally, Monsanto‘s data suggest

that cotton is rarely invasive in northern Australia, irrespective of inserted

insecticidal genes, because at most study sites invasiveness values were

considerably less than one. However, at the three disturbed, nutrient-rich

sites at which boll production was high (see above), indices of invasiveness

approached, or exceeded one. At two of these sites, the index was greater

than one for Bollgard II® cotton, but marginally less than one for

conventional cotton, implying that if the habitat is suitable, Bollgard II®

cotton may be more invasive than conventional cotton. Suitable habitats are

likely to be nutrient-rich, with an adequate supply of fresh water and

protected from other limiting environmental variables such as fire.



321. The risk of Bollgard II® cotton spreading as a problematic weed in the

natural environment is low because soil moisture and/or nutrients,

particularly phosphorous, are likely to limit germination, growth and fruit

production. Even in potentially suitable habitats within the natural





CHAPTER 6 WEEDINESS

environment such as creek or river banks, Bollgard II® cotton is unlikely to be

more problematic than conventional cotton, or existing feral cotton

populations, unless nutrient levels are increased significantly. Naturally,

most Australian soils, including those of northern Australia, have low levels

of key nutrients, particularly phosphorous.



322. It should be noted that while indices of invasiveness can be derived, the

ability of a plant to invade a community is a function of both its ‗invasiveness‘

and the community‘s ‗invasibility‘ (Lonsdale 1999), which itself may be

affected by community diversity, disturbance and various other

environmental variables. Monsanto‘s index of invasiveness does not account

for community invasibility, since study plots were cleared of competing

vegetation. The invasiveness of Bollgard II® cotton in specific, high-nutrient,

low-competition habitats, is therefore likely to reflect a ‗worst case scenario‘.

Moreover, it underscores the conclusion of several similar studies with

genetically modified insecticidal plants, that the selective advantage of these

plants is only manifested at sites characterised by particular combinations of

plant competition, herbivory or growth-limiting resources such as soil

nutrients or space (Crawley et al. 1993; Bergelson 1994; Stewart et al. 1997).



HERBICIDE RESISTANCE



323. There is a significant likelihood that selection of glyphosate-resistant

weeds in Bollgard II®/Roundup Ready® cotton crops could eventually occur.

Glyphosate-resistant weeds have already been identified, including Lolium

rigidum (annual ryegrass) in Australia and California and Eleusine indica

(goose-grass) in Malaysia. These weeds arose after repeated annual

application of glyphosate on conventional crops over many years.



324. The likelihood of this occurring would be minimised by the

implementation of effective farm management plans that avoid overuse of

glyphosate, and ensure ongoing monitoring and destruction of any

glyphosate-resistant weeds that do occur.



325. The potential for eventual unintended ‗stacking‘ of different herbicide

tolerances in a single plant, or for overuse of glyphosate if other

Roundup Ready® crops were used in rotation, would also be minimised if

appropriate management practices for the crops were in place. This is not a

problem that needs to be addressed for the current proposal, since no other

varieties of herbicide-tolerant cotton or other Roundup Ready® crop varieties





CHAPTER 6 WEEDINESS

are commercially available in Australia. It is however a factor that would

need to be considered for any future proposed releases of Roundup Ready®

crops or cotton with tolerance to herbicides other than Roundup.



326. The NRA sets limits on the new use of herbicides to manage the risk of

developing herbicide-resistant weeds through inappropriate use of herbicides

(see Chapter 8). In addition, users of Monsanto‘s Roundup Ready® and

Bollgard II®/Roundup Ready® cotton must comply with the company‘s

Technology User Agreement, which requires the adoption of an integrated

weed management strategy, to limit the development of herbicide resistance.

Integrated weed management in Monsanto‘s GM glyphosate resistant cottons

will significantly reduce the likelihood of developing glyphosate resistance in

weed species (Roberts and Charles, in press).



C: Conclusions regarding weediness



327. It is concluded that the risk of Bollgard II® or Bollgard II®/

Roundup Ready® cotton establishing as a weed in the southern

cotton-growing regions of NSW and Queensland is low, and not likely to be

greater than that of conventional cotton GM insecticidal INGARD® cotton, or

Roundup Ready® cotton.



328. The risk of Bollgard II® or Bollgard II®/ Roundup Ready® cotton

establishing as a weed in the cotton-growing regions of northern WA and the

NT is also likely to be low, however further information is required before

this can be determined conclusively. Nevertheless, it is considered that the

risks of cotton spreading as a weed could be managed to an acceptable level

by implementing various strategies to minimise the spread and persistence of

the GM cotton in the environment in northern WA and the NT.



329. The Regulator has imposed licence conditions to limit the planting of the

GM cotton in northern Australia, and to ensure that the GM cotton does not

spread from the release site, or persist at the site after harvest, thus reducing

the potential for the GM cotton to establish as a weed outside the release site

in northern Australia (see Chapter 9, Section 3).



330. Provision of additional information relating to potential environmental

risks in northern Australia will also be a condition of the licence and this

information would be required before commercial release could be extended

to the cotton-growing regions of WA and the NT (see Chapter 9 for details).





CHAPTER 6 WEEDINESS

331. There is some potential for development of herbicide-resistant weeds if

the crop-herbicide combination is used inappropriately. A herbicide

resistance management plan is required to minimise this risk. Prior to the

commercial release of Roundup Ready® cotton in 2000, a crop management

plan was developed in consultation with the Transgenic and Insect

Management Strategy (TIMS) Committee of the Australian Cotton Growers

Research Association. The NRA has responsibility for setting registration

conditions for the use of glyphosate on cotton crops, including herbicide

resistant management conditions.



332. There are also potential adverse effects on agricultural systems that

could arise from the development of insects resistant to Bollgard II® cotton.

Implementation of an insect resistance management plan (see Chapter 8) will,

therefore, be required as part of the licence. The possibility of interference

between the insect resistance and herbicide resistance management plans was

considered, however it has been concluded the management issues for the two

traits are independent of each other.









CHAPTER 6 WEEDINESS

CHAPTER 6 WEEDINESS

CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER

ORGANISMS



333. In general terms, the types of hazards that have been considered here are

whether the transfer of the genes introduced into INGARD®, Bollgard II® or

Bollgard II®/Roundup Ready® cotton to other organisms could result in the

production of insecticidal or herbicide-tolerant weeds. If this was to occur,

there may be potential for such weeds to compete with native flora, thereby

reducing biodiversity. The possibility of whether gene transfer might lead to

antibiotic-resistant pathogens, with potential to harm human or animal

health, is also considered.



334. The potential hazards are addressed in the following sections, with

respect to:

 other plants (Section 1 of this Chapter); and

 other organisms (Section 2 of this Chapter).





SECTION 1 TRANSFER OF INTRODUCED GENES TO OTHER PLANTS



A: Nature of the gene transfer hazard



TRANSFER OF GENES TO OTHER CULTIVATED OR FERAL COTTON



335. Transfer of the introduced genes to other cotton plants would present the

same hazards and have the same potential impacts as the presence of the

genes in Bollgard II® or Bollgard II®/Roundup Ready® cotton (see Chapters

5, 6 and 8). However, if transfer occurred to other cultivated or feral cotton,

this would further increase the possibility that the genes could spread in the

environment, with flow-on impacts depending on the nature of the gene and

the species to which it transfers.





TRANSFER OF GENES TO OTHER PLANT SPECIES



336. Transfer of the introduced genes into other plant species, in particular to

native flora, may have adverse effects on biodiversity. Other potential

hazards specific to the transferred gene sequences that were considered are:









CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 97

 Insecticidal genes:

whether plants could become resistant to lepidopteran insects. This

could confer a fitness advantage on plants normally controlled by

these insects, and could result in increased weediness. There could

also be impacts on the lepidopteran insect populations, or specialist

predators and parasitoids that feed on them.

 Antibiotic resistance marker genes:

whether plants could become resistant to the antibiotics. This

would only have an impact if the antibiotics were used on the plants.

 uidA (GUS) marker gene:

whether plants would produce the GUS protein.



 Herbicide tolerance gene:

whether plants could become resistant to glyphosate. This would

have an impact only if the plant is controlled by glyphosate, on the

farm or as a weed in the environment.

 CaMV 35S promoter and other regulatory sequences:

If gene transfer did occur, there could be unintended or unexpected

effects if the introduced regulatory sequences altered the expression

of endogenous plant genes. If such perturbation of normal plant

gene expression did occur, the impact would depend on the

phenotype.



Some of these sequences are derived from plant pathogens

(cauliflower mosaic virus, figwort mosaic virus, Agrobacterium

tumefaciens). The possibility should also be considered that they

might have pathogenic properties.



B: Likelihood of the gene transfer hazard occurring



TRANSFER OF GENES TO OTHER CULTIVATED OR FERAL COTTON



337. The most likely means by which the inserted genes could be transferred

to other cotton plants is by a GM cotton plant cross-pollinating (outcrossing

to) another cotton plant. For a detailed consideration of the likelihood of this

occurring, including an overview of the pollination biology of cotton, see the

accompanying document, ‗The Biology and Ecology of Cotton







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 98

(Gossypium hirsutum) in Australia‘ (OGTR 2002), available on the OGTR

website.



338. In summary, a low level of transfer to other cotton in a cropping

situation would be likely. As is currently the case for commercially released

GM INGARD and Roundup Ready cotton, no measures are proposed to

limit outcrossing and no specific segregation measures will be in place, other

than standard measures for seed production. Conventional farming

practices, such as the use of certified (pure) seed will ensure any

contamination is kept to a minimum;



339. Transfer to feral naturalised cotton populations is unlikely, because of

the geographic distances between these feral populations and the

cotton-growing regions, particularly in the cotton-growing regions of NSW

and QLD. In northern Australia measures will be imposed to limit gene flow

(see Chapter 9).



TRANSFER OF GENES TO OTHER PLANT SPECIES



340. There is effectively zero probability of transfer to any other plant

species, even for the most closely related native Australian Gossypium species

(OGTR 2002). This is due both to genetic incompatibility and, for most of

the native Gossypium species, their limited geographic distribution relative to

the cotton-growing regions.



C: Conclusions regarding gene transfer to other plants

In summary:

 The likelihood of some gene transfer from the GM cotton to

cultivated cotton is high, but the overall frequency of out-crossing

would be very low. This would not pose any risks additional to the

negligible risks posed by Bollgard II® or

Bollgard II®/Roundup Ready® cotton itself, covered in detail in this

document. Conventional farming practices, such as the use of

certified (pure) seed will ensure any contamination is kept to a

minimum;

 The potential for transfer of the introduced genes to wild or native

cotton is functionally zero because of the geographical isolation and

genetic incompatibility with the native species; and







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 99

341. The conclusions with respect to the specific transferred gene sequences

are as follows:

 Insecticidal genes:

It is possible that if these genes were transferred to feral or

cultivated cotton, the plants might have a survival advantage in

regions where lepidopteran insect predation limits their growth or

regulates their populations. Consideration of data on the potential

weediness of Bollgard II® and Bollgard II®/Roundup Ready® cotton

(see Chapter 6) suggests, however, that in most circumstances,

including in natural ecosystems, this is unlikely. The distribution

of cotton is determined largely by soil type and soil moisture, more

so than by insect pressure. In specific high-nutrient habitats where

weedy volunteers may occur, these will be managed by removing the

plants or by spraying with an appropriate herbicide.



 Antibiotic resistance genes:

There would be no adverse impacts even if gene transfer occurred,

since antibiotics are not generally used on plants outside of the

laboratory. Streptomycin is used in some other countries to control

fire blight, a bacterial disease of fruit trees. However, fire blight

does not occur in Australia, is not a disease of cotton, and plants in

Australia are therefore not treated with streptomycin.



 uidA GUS marker gene:

There would be no adverse consequences even if gene transfer

occurred. GUS is not likely to be toxic or allergenic to other

organisms, or to increase the weediness of the cotton (Chapters 5

and 6).

 Herbicide tolerance gene:

There would be no adverse consequences even if outcrossing to

cotton occurred, since cotton species are not regarded as weeds in

Australia and are not controlled by glyphosate on the farm or in the

natural environment. As noted above, the likelihood of transfer to

plants other than cotton that might be controlled by glyphosate is

effectively zero.







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 100

 CaMV 35S promoter and other regulatory sequences:

The probability of a hazard arising due to outcrossing of these

sequences to other plants is remote. Plants are already exposed in

nature to the bacteria and viruses from which these sequences are

derived.



Although some of the regulatory sequences transferred to the plants

are derived from plant pathogens, they only represent a very small

proportion of the pathogen genome. The sequences are not, in

themselves, infectious or pathogenic. It should be noted that CaMV

is already ubiquitous in the environment and in the human diet

(Hodgson 2000a).



SECTION 2 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS

(MICROORGANISMS AND ANIMALS)



A: Nature of the gene transfer hazard



342. The following potential hazards, with respect to the transfer of specific

gene sequences, were considered:

 Insecticidal genes:

This would not present a hazard to human health or the

environment.



 Antibiotic resistance genes:

Transfer of the genes to animals (including humans) or

microorganisms other than bacteria (such as viruses) would not

present a hazard to human health or the environment. However,

bacteria that acquired the antibiotic resistance gene(s) could become

resistant to those antibiotics. The consequences of this would

depend on:



 the pathogenicity of the microorganism;



 the use and significance of the antibiotic(s) in clinical

and/or veterinary practice;







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 101

 whether resistance to the antibiotic(s) is already widespread

in the microbial population.



 uidA marker gene:

Transfer of the gene to animals (including humans) or

microorganisms other than bacteria (such as viruses) would not

present a hazard to human health or the environment.



 Herbicide tolerance gene:

This would not present a hazard to human health or the

environment.



 CaMV 35S promoter and other regulatory sequences:

If gene transfer occurred, there could be unintended or unexpected

effects if the introduced regulatory sequences alter the expression of

endogenous genes. If such perturbation of normal gene expression

occurred, the impact would depend on the resultant phenotype.



Some of these sequences are derived from plant pathogens

(cauliflower mosaic virus, figwort mosaic virus, Agrobacterium

tumefaciens). The possibility should be considered that they might

have pathogenic properties.



The possibility that the regulatory sequences could recombine with

the genome of another virus infecting the plants to create a novel

recombinant virus should also be considered.



B: Likelihood of the gene transfer hazard occurring



343. The likelihood of genes transferring from cotton to other organisms has

been considered in detail in the accompanying document, ‗The Biology and

Ecology of Cotton (Gossypium hirsutum) in Australia‘ (OGTR 2002),

available on the OGTR website. In summary, the transfer of the introduced

genes from INGARD®, Bollgard II® or Bollgard II®/Roundup Ready cotton

to humans or other animals, or microorganisms including bacteria and

viruses is extremely unlikely.







CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 102

C: Conclusions regarding gene transfer to other organisms



344. In summary, the likelihood of transfer of the introduced genes to other

organisms is negligible, but even if such transfer occurred would be unlikely

to pose any hazard to human health and safety or the environment.



345. Horizontal gene transfer from plants to animals (including humans) or

microorganisms is extremely unlikely. It should be noted that the cry1Ac

and cry2Ab insecticidal genes are already widespread in the environment

(they were originally isolated from a common soil bacterium, Bacillus

thuringiensis). The nptII, uidA and aad genes are also prevalent in naturally

occurring bacteria found in soil and in animal and human digestive systems.

The nptII and aad genes occur naturally on transmissible genetic elements

(transposons and plasmids) that are readily transferable between bacterial

species (Flavell et al. 1992; Langridge 1997; Pittard 1997; US FDA 1998;

Draft GuidAnce Document on Use of Antibiotic Resistance Marker Genes in

Transgenic Plants, 1998). Transfer of the genes from these naturally

occurring bacteria, through well documented mechanisms for horizontal

transfer between bacteria (Nielsen et al. 1998; Nielsen KM 1998;

Doblhoff-Dier et al. 2000), is far more likely than transfer of the same genes

from GM cotton.



346. The conclusions with respect to the specific gene sequences are as

follows:

 Insecticidal genes:

There would be no adverse consequences even if gene transfer

occurred.



 Antibiotic resistance genes:

Transfer of these genes to organisms other than bacteria would not present a

hazard, since the antibiotics in question are only used to treat or prevent

bacterial infections. Horizontal transfer to bacteria is also extremely

unlikely and is considered to pose negligible risks to human health or the

environment for the following reasons.



Bollgard II® and Bollgard II®/Roundup Ready® cotton contain genes that

confer resistance to neomycin, kanamycin, streptomycin and spectinomycin.

None of these antibiotics are extensively used in clinical medicine.





CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 103

Streptomycin was formerly used in the treatment of tuberculosis, but is not

routinely used today because of its toxicity and the relatively high frequency

at which streptomycin-resistant mutants emerge. Only neomycin and

kanamycin are used in veterinary practice, and alternative antibiotics are

readily available.



The use of antibiotic-resistance markers in genetically modified plants and

microorganisms to be released into the environment has been researched and

reviewed extensively. It has been concluded that the presence of

kanamycin-resistance genes in genetically modified plants represents no

significant risk to biosafety (Flavell et al. 1992; Langridge 1997; Pittard 1997;

FDA 1998b; JETACAR 1999). Flavell et al. (1992) note that the human

health analyses need to be viewed against the knowledge that humans

continually ingest kanamycin-resistant microorganisms. The diet, especially

raw salad, is the major source: at a conservative estimate, each human ingests

1.2 x 106 kanamycin-resistant microorganisms daily. Previous concerns that

the NPTII protein may, itself be toxic or active in human or other animal

digestive systems have been effectively eliminated by the work Fuchs et al.

(1993b; 1993c).



The existence of the streptomycin/spectinomycin resistance gene on

transposons and plasmids found in both gram positive and gram negative

bacteria indicates its extensive distribution through the microbial world

(Shaw et al. 1993). Although this particular mechanism of resistance does

not occur in mycobacteria, resistance to streptomycin and spectinomycin as a

result of spontaneous mutations in genes encoding ribosomal RNA occurs at a

relatively high frequency because, unlike the enteric microorganisms,

mycobacteria contain only a single copy of such genes.



In summary, the incidence of naturally occurring bacterial strains resistant to

the antibiotics in question is already very high, and the antibiotic resistance

genes in these bacteria are often located on transmissible genetic elements

that are readily transferable between bacterial species. So, in the unlikely

event that the aad or nptII genes were transferred from INGARD®,

Bollgard II® or Bollgard II®/ Roundup Ready® cotton to a bacterium, this

would be unlikely to have any detectable impact on the existing level of

resistance in microbial populations. Furthermore, the antibiotics in question

are not of major clinical or veterinary significance.





CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 104

 uidA GUS marker gene:

There would be no adverse consequences even if gene transfer

occurred.



 Herbicide tolerance gene:

There would be no adverse consequences even if gene transfer

occurred.



 CaMV 35S promoter and other regulatory sequences:

There would be no adverse consequences even if gene transfer

occurred.



While Ho et al. (2000) have postulated that there are risks posed

through recombination of the CaMV 35S promoter with the

genomes of other viruses infecting the plants to create new viruses,

or of integration of the CaMV35S promoter into other species

causing mutations, cancer or reactivation of dormant viruses, these

claims have been challenged in the scientific literature (eg Hull et al.

2000; Morel & Tepfer 2000; Hodgson 2000b; Hodgson 2000c;

Tepfer 2002).



Although some of the regulatory sequences transferred to the plants

are derived from pathogens, the pathogens only infect plants. In

any case, the regulatory sequences only represent a very small

proportion of the pathogen genome and are not, in themselves,

infectious or pathogenic. It should be noted that CaMV is already

ubiquitous in the environment and in the human diet (Hodgson

2000a).









CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 105

CHAPTER 7 TRANSFER OF INTRODUCED GENES TO OTHER ORGANISMS 106

CHAPTER 8 INSECTICIDE RESISTANCE



A: Nature of the insecticide resistance hazard



347. Extensive cultivation of Bollgard II® cotton could potentially result in the

emergence of resistance to the Cry1Ac and Cry2Ab proteins in the target

species (Helicoverpa armigera and H. punctigera) and other susceptible

lepidopteran species feeding on cotton. This would result in a reduction of

the efficacy of Bollgard II® and Bollgard II®/Roundup Ready® cotton for

control of insect pests, and could also have impacts on the use of Bt microbial

sprays to control insects in other agricultural systems. Potential adverse

effects include attenuation of the benefits to the environment, and possibly

human health of growing Bollgard II® or Bollgard II®/Roundup Ready®

cotton or using Bt sprays. These benefits include the use of less chemical

insecticide on crops.



348. It should be noted that similar risks are also posed by the use of Bt

sprays or chemical insecticides in agricultural systems and, as such, are not

unique to the risk associated with cultivation of GM Bollgard II® cotton.

Although the Regulator has given detailed consideration to these risks,

ultimate responsibility for oversight of insect resistance management

requirements for GM insecticidal cotton currently lies with the National

Registration Authority (see part C of this Chapter).



B: Likelihood of the insecticide resistance hazard occurring



349. The emergence of insects resistant to the Cry1Ac and/or Cry2Ab protein

would almost certainly occur eventually if Bollgard II® or

Bollgard II®/Roundup Ready® cotton were grown widely without taking any

steps to deal with the problem. However, Bollgard II® cotton was developed

with the specific intention of reducing this risk (Gould 1998; Roush 1998b;

Benedect & Altman 2001).



350. The expression of two insecticidal proteins (‗pyramiding‘) in

Bollgard II® cotton is expected to delay selection of insects resistant to the

insecticidal proteins by a factor of 10 compared to INGARD® cotton (Roush

1994; Roush 1998a).



351. The two insecticidal genes are expressed over the duration of the

growing cycle. The increased insecticidal activity of the Bollgard II® cotton,





CHAPTER 8 INSECTICIDE RESISTANCE

relative to INGARD® cotton, especially during the later stages of the

cotton-growing season (see Chapter 2, section 5.2), is also expected to delay

the emergence of resistant insects (‗high dose strategy‘; Roush 1998a).



OCCURRENCE OF RESISTANCE IN INSECTS



352. Several studies have shown that resistance to Bt toxins can be selected in

the laboratory by repeated exposure of insects to the toxins (Peferoen 1997;

Tabashnik et al. 2000b). For example, two laboratory strains of Heliothis

virescens were selected to become resistant to Cry1Ac and other Bt derived

toxins (Gould et al. 1995a), and Akhurst et al. (2000) have isolated a

laboratory strain of the Australian Helicoverpa armigera that is resistant to

Cry1Ac. Moar et al. (1995) have selected strains of Spodoptera exigua

resistant to Cry1C and these insects were cross-resistant to Cry1Ab, Cry9C

and Cry2A as well as to a recombinant Cry1E-Cry1C fusion protein.

Recent studies have found evidence of cross-resistance between Cry1Ac and

Cry2A proteins in Cry1Ac-resistant strains of Pectinophora gossypiella,

Helicoverpa zea and H. armigera (Burd et al. 2000; Tabashnik et al. 2000a;

Akhurst 2001).



353. Bt resistance has also been studied in field populations of the

diamondback moth, Plutella xylostella (Tabashnik et al. 1990). The

diamondback moth, a major pest of cruciferous vegetables around the world,

receives frequent exposure to insecticides and shows extensive resistance to

most chemical insecticides in many growing areas. High levels of resistance

to Cry1A toxins have been found in populations of the diamondback moth

from the Philippines, Hawaii, Florida and Asia (Tabashnik et al. 1990;

Tabashnik 1994).



354. Resistance to Bt insecticide appears to be due to one (Tang et al. 1997a)

or at most a few genes (Tabashnik et al. 1992; Ferre et al. 1995; Gould et al.

1995a; Tabashnik et al. 1998). The mechanisms of insecticidal activity

include reduced binding of the toxin to the midgut (Tabashnik et al. 1994b;

Gould et al. 1995b; Tang et al. 1997b), slower interaction of gut proteinases

with the protoxin, or the absence of a major gut protein (Oppert et al. 1997).



355. Genetic crosses of the laboratory-selected insecticidal strains of

Heliothis virescens demonstrated that a major portion of the resistance in this

case was encoded by a single gene (or a set of linked genes) with mostly

recessive inheritance (Gould et al. 1995a; Gould et al. 1997; Tabashnik et al.





CHAPTER 8 INSECTICIDE RESISTANCE

1997a). Studies of resistance in insects from field populations suggest that

the common mode of resistance is characterised by a high level of resistance

(over 500-fold), reduced toxin binding and a recessive mutation. However,

there appear to be other modes of resistance which are not recessive and

which are not associated with reduced toxin binding (Moar et al. 1995;

Tabashnik et al. 1997b).



356. Gould et al. (1997) estimated the frequency of alleles for resistance in

field populations of H.virescens as 1.5 x 10-3. Genetic models indicate that a

recessive allele present at this frequency could lead to rapid evolution of

resistant populations if Bt toxin-producing cotton is grown without adequate

refuges for toxin-susceptible larvae (Roush 1994; Gould et al. 1997). A

recessive allele that confers resistance to the Cry1Ac toxin in pink bollworm

in Arizona cotton fields has been reported at frequencies roughly 100-fold

higher than those reported by Gould et al. (1997) for resistance to H.

virescens. However, the frequency of this allele did not increase significantly

between 1997 and 1999, even though the Bt cotton was grown in over half the

100 000 hectares planted to cotton. Moreover, the efficacy of the Bt cotton

against the target pests remained extremely high (Tabashnik et al. 2000b).



357. Reversal of resistance in laboratory strains of diamondback moth

derived from resistant field populations has been observed when exposure to

Bt insecticide was discontinued over many generations. Reversal of

resistance was associated with restoration of binding of Cry1Ac to

brush-border membrane vesicles (Tabashnik et al. 1994a).



RESULTS FROM MONITORING FOR RESISTANT INSECTS IN AUSTRALIA



358. No monitoring has yet been conducted to investigate the incidence of

resistance to the Cry2Ab protein in Australian Helicoverpa populations

because there has as yet been only very limited exposure to this protein, in

trials of Bollgard II® cotton. In commercial Bt pest control products such as

Dipel® and Crymax®, Cry2Ab protein is not expressed due to an inefficient

expression of the Cry2Ab gene (Dankocsik et al. 1990). The only exposure of

Helicoverpa species in Australia to the Cry2Ab protein has been through

limited field trials Bollgard II® cotton conducted since 1999.



359. Monitoring for resistance to Bt microbial sprays containing the Cry1Ac

toxin in field populations of Helicoverpa armigera and H. punctigera has been

carried out by the NSW Department of Agriculture and the Australian





CHAPTER 8 INSECTICIDE RESISTANCE

Cotton Research Institute since 1993. Larvae are collected from the field

and fed a diet containing Bt toxins at a level (the discriminatory dose) that is

calibrated to kill most of the population. Increased survival at the

discriminatory dose would indicate increased resistance in the insect

population. No changes in susceptibility to discriminating doses of

commercial Bt sprays have been recorded in any of the Australian field

populations of H. armigera and H. punctigera collected from cotton between

1993 and 2000 (Holloway & Dang 2000). These results indicate that there

has been no shift towards insect resistance in INGARD® crops since the

commercial release of INGARD® cotton in 1996, and provide evidence for the

efficacy of the resistance management plans currently in place for INGARD®

cotton (see below).



360. During the 2000 - 2001 season the NSW Department of Agriculture

recorded an increase in resistance (from 7.1 to 9.2 %) of H. armigera larvae to

Cry1Ac. However, this apparent increase coincided with a change in the

bioassay method. The significance of this result is currently being

investigated by Monsanto and the NSW Department of Agriculture.

Preliminary analyses suggest that the differences in susceptibility are within

the limits of natural variation and therefore not significant.





C: Conclusions regarding insecticide resistance



361. Even though the Bollgard II® cotton is expected to significantly delay the

development of insecticide resistance compared with INGARD cotton (see

Part B of this Chapter), emergence of insects resistant to the Cry1Ac and/or

Cry2Ab protein would almost certainly occur unless preventative strategies

are implemented. Given the potentially unrestricted scale of the proposed

release, the likelihood of emergence of insects resistant to the Cry1Ac and/or

Cry2Ab proteins, as a result of the release, is considered significant.

Therefore, the licence will require implementation of insecticide resistance

management strategies, in accordance with the insecticide resistant

management requirements of the NRA, including obtaining approval from

the NRA before planting can proceed.



362. As a condition of registration by the NRA of INGARD® cotton, growers

are required to implement insecticide resistance management plans developed

by the Transgenic and Insect Management Strategy (TIMS) Committee of the

Australian Cotton Growers‘ Research Association. The plans are designed



CHAPTER 8 INSECTICIDE RESISTANCE

to minimise resistance development, and require growers to employ a number

of measures (see below).



363. An insecticide resistance management plan for Bollgard II® and

Bollgard II®/Roundup Ready® cotton is being developed currently by the

TIMS Committee of the Australian Cotton Growers‘ Research Association in

consultation with the NRA, but the plan is yet to be finalised. If it approves

the registration of Bollgard II® cotton, the NRA will require implementation

of the plan as a condition of registration. It is likely that different insecticide

resistance management plans will be required for releases in the NT and WA,

because the climate of these potential cotton-growing regions differs from

that in the southern cotton-growing areas. This is likely to affect the number

of generations of insect pests that are produced each year, with associated

implications for the potential development of resistance.



INSECTICIDE RESISTANCE MANAGEMENT PLAN



364. The insecticide resistance management plans are designed to minimise

resistance development, and require growers to employ a number of

measures. These are likely to include requirements to:

 limit the total area planted to the GM cotton;

 plant refuges of non-GM unsprayed cotton or other plants where

the insect pests can breed freely (this provides a population of

susceptible insects to dilute out resistance genes if these develop in

the insect pests);

 plant by a certain date;

 control volunteer cotton;

 after harvest, to cultivate soil to prevent Helicoverpa larvae in the

soil surviving to adulthood (‗pupae-busting‘, see OGTR 2002).









CHAPTER 8 INSECTICIDE RESISTANCE

CHAPTER 9 RISK MANAGEMENT PLAN



365. This part of the document recaps the main conclusions from the risk

assessment relating to risks to human health and safety or the environment,

and details the risk management plan developed by the Regulator to manage

these risks.





SECTION 1 SUMMARY OF RISK ASSESSMENT CONCLUSIONS



366. It has been concluded that the proposed release of Bollgard II® and

Bollgard II®/Roundup Ready® cotton in NSW and QLD will not pose any

additional risks to human health and safety or to the environment in

cotton-growing areas of NSW and QLD. However, based on the available

evidence it has not been possible to determine conclusively that the proposed

release would not pose an environmental risk in cotton-growing areas of NT

and WA. The main conclusions from the risk assessment are that:

 Bollgard II® and Bollgard II®/Roundup Ready® cotton are not likely

to prove more toxic or allergenic to humans or other organisms

(other than lepidopteran insects including the target pests) than

conventional cotton.

 the risk of Bollgard II® or Bollgard II®/ Roundup Ready® cotton

establishing as a weed in southern Australia is low, and not likely to

be greater than that of conventional cotton. In northern Australia,

the risk of Bollgard II® or Bollgard II®/Roundup Ready® cotton

establishing as a weed is also likely to be low, however further

information is required before this can be determined conclusively;

 there is some potential for development of herbicide-resistant weeds

if the crop-herbicide combination is used inappropriately;

 the likelihood of some gene transfer from the GM cotton to

cultivated cotton is high, but the overall frequency of out-crossing

would be very low. This would not pose any risks additional to the

negligible risks posed by Bollgard II® or

Bollgard II®/Roundup Ready® cotton itself, covered in detail in this

document. Conventional farming practices, such as the use of

certified (pure) seed will ensure any contamination is kept to a

minimum;







CHAPTER 9 RISK MANAGEMENT PLAN

 the potential for transfer of the introduced genes to wild or native

cotton is functionally zero because of the geographical isolation and

genetic incompatibility with the native species;

 the likelihood of transfer of the introduced genes to other organisms

is negligible, but even if such transfer occurred it would be unlikely

to pose any hazard to human health and safety or the environment;

and

 the risk of development of target insects resistant to the insecticidal

proteins is very low, due to the presence of two insecticidal proteins

and the development of insecticide resistance management plans

that are tailored to different cotton-growing environments.

SECTION 2 RISK MANAGEMENT PLAN



Section 2.1 Risk of toxicity or allergenicity



367. It is not considered necessary to include any specific management

strategies in the risk management plan in relation to the potential toxicity or

allergenicity of the cotton, since the risks are negligible. However, the

licence contains a general condition requiring the licence holder to notify the

Regulator of any adverse effects on human health and safety (for example,

allergic reactions to the cotton through occupational exposure), or to the

environment. It should be noted that FSANZ (formerly ANZFA) has

previously approved the use in food of oil and linters from Roundup Ready

and INGARD cotton, and has concluded in two previous assessments that

products from Bollgard II® cotton are as safe as those from conventional

cotton. Final approval from FSANZ would be required before oil and linters

from Bollgard II® or Bollgard II®/Roundup Ready® cotton would be

permitted for use in human food.



Section 2.2 Risks of insecticide resistance



368. There is some potential for the development of insects resistant to the

insecticidal proteins present in the GM cotton. However, the NRA sets limits

on the total area (hectares) that may be planted to GM insecticidal cottons,

and other conditions in relation to compliance with insecticide resistance and

herbicide use management plans.









CHAPTER 9 RISK MANAGEMENT PLAN

Section 2.3 Risks of weediness or gene transfer



369. The risk of transfer of genes from the GM cotton to organisms other than

cotton, including the risk of transfer to native Australian cottons, is

considered to be negligible. The risk of it spreading as a weed in southern

Australia is low and no management strategies are considered necessary to

manage this risk for releases in southern Australia. Release of Bollgard II®

and Bollgard II®/Roundup Ready cotton without specific management

conditions (i.e. general release) will be permitted south of latitude 20º South,

covering all current cotton-growing regions in NSW and Queensland. This

represents an extension above the area approved for general release of

INGARD and Roundup Ready cotton, i.e. south of latitude 22º South (see

Chapter 2).



370. The initial general release of INGARD cotton was restricted to south of

latitude 26º South, and this was later extended to accommodate expansion of

the cotton industry in Queensland. The extension of the area approved for

general release of Bollgard II® and Bollgard II®/Roundup Ready cotton is to

cover subsequent expansions. However, the cotton-growing regions of WA

and the NT, where it is considered that the main risks relating to weediness

and out-crossing to native species occur, are still excluded.



371. Extension to latitude 20º South does not increase the potential for

exposure of native Gossypium species or feral cotton populations to the GM

cotton (data from Australia‘s Virtual Herbarium:

http://www.cpbr.gov.au/avh.html and the Northern Territory Herbarium).



372. Licence conditions will be imposed for releases above latitude 20º South

to manage the potential weediness of the GM cotton in northern Australia, by

limiting gene flow and the spread and persistence of the GM cotton in the

environment. This will include requirements to:

 isolate the GM cotton crop from other cotton by at least 50 metres

or to surround the release sites with 20 metre buffer rows of

conventional cotton;

 prohibit the cultivation of the GM cotton within 100 metres of

known feral cotton populations;









CHAPTER 9 RISK MANAGEMENT PLAN

 after the release, destroy any viable material not required for

subsequent trials (which would require separate licences), unless

exported or used for stockfeed; and

 after the release, monitor for and destroy any cotton plants

(volunteers) that germinate or regrow on the release site for a

period of at least 12 months, during which no cotton could be grown

on the site; and

 where viable seed is used for stockfeed north of latitude 20º South,

to monitor stockyards and relevant transport routes for volunteers

and destroy them.



373. The licence will also contain other conditions relating to the harvest,

cleaning of equipment and locations, transport and post-harvest use of the

sites, including management practices specifically designed to reduce the seed

bank of the GMO. The conditions also include a requirement for the

applicant to have in place contingency plans to deal with any unintended

release of the GMOs outside the release sites in northern Australia.



374. The NRA sets limits on the new use of herbicides to manage the risk of

developing herbicide-resistant weeds through inappropriate use of herbicides.

TIMS committee of the Australian cotton Growers‘ association annually

review and audit Roundup Ready® cotton growers for any shifts in herbicide

resistance. In addition, users of Monsanto‘s Roundup Ready® cotton must

comply with the company‘s Technology User Agreement. This agreement

requires the adoption of an integrated weed management strategy, which

effectively limits the development of glyphosate resistant weeds (Roberts and

Charles, in press).



375. The proposed licence conditions, and the reason behind them, are set out

in detail in Section 3 of this Chapter and Appendix 1.



Section 2.4 General licence conditions



376. In addition to the specific risk management conditions discussed in

Section 3 of this Chapter, the licence issued by the Regulator contains a

number of general conditions including statutory conditions relating to

requirements under Sections 61 to 65 of the Act. These conditions apply to

all licences issued by the Regulator, and may also be relevant to risk

management. For example, there are conditions that will:





CHAPTER 9 RISK MANAGEMENT PLAN

 identify the persons or classes of person covered by the licence;

 specify the authorised dealings; and

 require the applicant to:

 inform people covered by the licence of their obligations under the

licence;

 allow access to the release sites by the Regulator, or persons

authorised by the Regulator for the purposes of monitoring or

auditing;

 inform the Regulator if the applicant becomes aware of any

additional information about risks to human health or safety or to

the environment, any unintended effects of the release, or any

contraventions of the licence conditions; and

 ensure appropriate training for persons covered by the licence.





Section 2.5 Monitoring and enforcement of compliance by the OGTR



377. It should be noted that, as well as imposing licence conditions, the

Regulator has additional options for risk management. The Regulator has

the legislative capacity to enforce compliance with licence conditions, and

indeed, to direct a licence holder to take any steps the Regulator deems

necessary to protect the health and safety of people or the environment. The

OGTR also independently monitors trial sites to determine whether the

licence holder is complying with the licence conditions, or whether there are

any unforseen problems.



378. The OGTR undertakes risk profiling during the planning of monitoring

activities. For these releases, factors which may represent higher risk,

including times of planting, flowering and harvest of the GMO, and the likely

times of germination of cotton volunteers will be taken into account in the

timing of monitoring visits.





SECTION 3 SPECIFIC RISK MANAGEMENT LICENCE CONDITIONS



379. The licence conditions set out in Appendix 1 are intended to manage the

identified risks, largely through preventing dissemination in northern

Australia of the GMOs or their genetic material into habitats in which the





CHAPTER 9 RISK MANAGEMENT PLAN

potential weediness of Bollgard II®, Bollgard II®/Roundup Ready® and feral

cotton may be increased.



380. To ensure that any future limited and controlled releases of GM cotton

can be conducted under appropriate levels of containment, the conditions

include a requirement to develop an agreed research program to collect data

relating to the effectiveness of containment measures, as part of the release.

The proponent will also be required to develop a research program to provide

information on potential environmental impacts of the GM cotton,

particularly in northern Australia.



381. The proposed conditions also include a requirement for the applicant to

have in place contingency plans to deal with any unintended release of the

GMOs outside the release sites in northern Australia.



382. Detailed reasons for the individual licence conditions are set out in

Appendix 2.



383. Monsanto will be required, under licence conditions, to be proactive in

reviewing and assessing any new information that comes to light about the

risks and the efficacy of the proposed management strategies during the

course of the release. Any licence is able to be varied at any time to add new

conditions, for instance to manage any new risks that are identified, or to

improve the existing management strategies.



384. The Regulator will also be proactive in reviewing any new information

about risks of the proposed release and may amend licence conditions on the

basis of this. Under section 68 of the Act the Regulator may suspend or

cancel a licence if a condition of the licence has been breached or if the

Regulator becomes aware of risks that the licence holder is not adequately

managing.



385. Finally, it should be noted that, the Regulator is reviewing all licence

conditions for licences carried over from the voluntary system under the

transitional arrangements set out in the Act. If as a result of this review, new

information becomes available about risks relevant to the proposed release,

any licence issued to Monsanto would be amended if necessary.









CHAPTER 9 RISK MANAGEMENT PLAN

CHAPTER 9 RISK MANAGEMENT PLAN

CHAPTER 10 CONSIDERATION OF ISSUES RAISED IN PUBLIC

SUBMISSIONS



386. Comments received in written submissions on the application and risk

assessment and risk management plan were very important in shaping the

final risk assessment and risk management plan and in informing the

Regulator‘s final decision on the application.



387. The OGTR received 48 submissions from the public, which are

summarised in Appendix 3 along with an indication of where identified risks

to human health and safety and the environment were considered in the risk

assessment and risk management plan. In summary, the submissions

suggested that the following issues should be addressed:

 toxicity and allergenicity of the GM cotton, for humans and other

organisms (see Chapter 5);

 weediness of the GM cotton, particularly in northern Australia, and

the potential for development of herbicide-resistant weeds (see

Chapter 6);

 potential of transfer of the introduced genes, to other plants

(particularly native Gossypium species in northern Australia) and

other organisms and the subsequent impact (see Chapter 7); and

 the potential for development in target pests resistance to the

insecticidal toxins in the cotton (see Chapter 8);



388. It is important to note that the legislation requires the Regulator to base

the licence decision on whether risks posed by the proposed dealings are able

to be managed so as to protect human health and safety and the environment.

Matters in submissions that do not address these issues and/or concern

broader issues outside the objective of the legislation can not be considered in

the assessment process. In most instances, as determined in the extensive

consultation process that led to the development of the legislation, they fall

within the responsibilities of other authorities.



389. For example, many submissions raised issues that related to matters that

are the responsibility of other regulatory authorities, in particular, labelling

and safety of foods derived from GMOs, and the use and safety of herbicides.

These are issues that are dealt with by Food Standards Australia New

Zealand (FSANZ, formerly the Australia New Zealand Food Authority) and





CHAPTER 10 CONSIDERATION OF ISSUES RAISED IN PUBLIC SUBMISSIONS 121

the National Registration Authority for Agricultural and Veterinary

Chemicals (NRA) respectively. Further information about food safety

assessments and food labelling and the use and safety of herbicides are

available from FSANZ and the NRA:

Food Standards Australia New Zealand

PO Box 7186

Canberra Mail Centre ACT 2610

Phone: (02) 6271 2222

Fax: (02) 6271 2278

E-mail: info@foodstandards.gov.au

http://www.foodstandards.gov.au





National Registration Authority for Agricultural and Veterinary Chemicals

PO Box E240

KINGSTON ACT 2604

Phone: (02) 6272 5158

Fax: (02) 6272 4753

Email: nra.contact@nra.gov.au

http://www.nra.gov.au



390. In addition, issues such as marketability and trade implications posed by

the commercialisation of GM crops in Australia do not fall within the scope of

the evaluations. These matters are being actively considered by the

Commonwealth, State and Territory Governments (both individually and

through forums such as the Primary Industries Ministerial Council and its

Plant Industries Committee); Agriculture, Fisheries and Forestry Australia

(AFFA) through its Supply Chain Management of GM Products project; and

by industry through groups such as the Gene Technology Grains Committee.



391. The issues raised in relation to the proposed release and risks to human

health and safety and the environment in the submissions were considered

carefully, and weighed against the body of current scientific information, in

reaching the conclusions set out in this document. A summary of public

submissions and an indication of where such issues were taken into account is

provided at Appendix 3.









CHAPTER 10 CONSIDERATION OF ISSUES RAISED IN PUBLIC SUBMISSIONS 122

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APPENDIX 1 SPECIFIC LICENCE CONDITIONS





Note in relation to herbicide and insect resistance



The Gene Technology (Consequential Amendments) Act (2000) requires the

National Registration Authority for Agricultural and Veterinary Chemicals

(NRA) to consult the Gene Technology Regulator for the purposes of making

certain decisions regarding registration or issuing a permit for a chemical

product that is or contains a genetically modified product.



The genetically modified organisms referred to in this licence fall into the

Agricultural and Veterinary Chemicals Code (1994) definition of an

agricultural chemical product, due to their production of insecticidal

substances, and are therefore subject to regulation by the NRA.



As some of these organisms are genetically modified to be resistant to a

herbicide, the NRA will assess the new use pattern of the herbicide used in

connection with these organisms.



The NRA may impose conditions in connection with the insecticidal activity of

these genetically modified organisms, including specifying maximum areas

for release and for the purpose of managing weeds resistant to the herbicide

used.



The OGTR has not, in this licence, imposed conditions in relation to insect

resistance management or herbicide resistance management in relation to

agricultural practices. These matters are expected to be satisfactorily

managed in the context of the NRA scheme.









PART 1

This instrument, including its attachments, is a licence authorising dealings

involving the intentional release of GMOs into the environment. It is issued

by the Gene Technology Regulator (the Regulator) pursuant to the Gene







APPENDIX 1

Technology Act 2000 (Cth).



Holder of licence

1.1 The holder of this licence (‗the licence holder‘) is Monsanto Australia

Ltd



Project Supervisor

2.1 The Project Supervisor in respect of this licence is the person identified

at ‗Project Supervisor‘ at Attachment A.

Persons covered by licence

3.1 The persons covered by this licence are the licence holder and

employees, agents or contractors of the licence holder and other persons who

are, or have been, engaged to undertake any activity in connection with a

GMO grown in a Location pursuant to this Licence (including growing,

harvesting, ginning or transportation of the GMO, and persons who feed

ginned GM cotton to stock).



(Explanatory Note: Each person covered by this licence is a ‘person covered by

a GMO licence’ for the purposes of the Gene Technology Act 2000 (Cth)).



Description of GMO covered

4.1 The GMO covered by this licence (‗the GMO‘) is identified and

described at ‗GMO Description‘ at Attachment B.



Dealings authorised by licence

5.1 This licence authorises the licence holder and persons covered by the

licence to conduct certain limited dealings with the GMO subject to the

limitations on dealing with the GMO that are contained elsewhere in the

conditions in this licence.



Period covered by licence

6.1 This licence remains in force until it is cancelled or surrendered. No

dealings with the GMO are authorised during any period of suspension.









APPENDIX 1

(Note: If adverse effects are reported by the Licence holder or detected through

the research program, these must be reported to the Gene Technology Regulator

immediately, who will then vary the Licence conditions to protect the health and

safety of people and the environment)









APPENDIX 1

PART 2



Interpretation and Definitions



Words and phrases used in this licence have the same meanings as they do in

the Gene Technology Act 2000 (the Act) and the Gene Technology Regulations

2001.



Words importing a gender include any other gender.



Words in the singular include the plural and words in the plural include the

singular.



Words importing persons include a partnership and a body whether

corporate or otherwise.



References to any statute or other legislation (whether primary or

subordinate) is to a statute or other legislation of the Commonwealth of

Australia as amended or replaced from time to time unless the contrary

intention appears.



Where any word or phrase is given a defined meaning, any other part of

speech or other grammatical form in respect of that word or phrase has a

corresponding meaning.



In this licence:



‘Clean’ (or ‘Cleaned’ or ‘Cleaning’) means, as the case requires:

(a) in relation to a Location or an area, the destruction of the GMO,

viable Material from the GMO, Pollen Trap plants or viable

Material from Pollen Trap plants in that Location or area, to the

reasonable satisfaction of the Regulator; or

(b) in relation to Equipment, the removal and destruction of the

GMO and viable Material from the GMO, Pollen Trap plants or

viable Material from Pollen Trap plants from the Equipment, to

the reasonable satisfaction of the Regulator.



‘Cotton’ means plants of the species Gossypium hirsutum L.







APPENDIX 1

‘Covered Vehicles’ means vehicles that use tight fitting covers to prevent

spillage of the seed transported in them (for example, vehicles that contain

seed in steel or aluminium bulk bins covered with tight, well fitting

weather-proof tarpaulins or similar).



‘Destroy’, (or ‘Destroyed’ or ‘Destruction’) means, as the case requires, killed

by one or more of the following methods:

(a) stalk pulling; or

(b) uprooting by ploughing; or

(c) burning; or

(d) treatment with herbicide; or

(e) hand weeding.



Note: ‘As the case requires’ has the effect that, depending on the circumstances,

one or more of these techniques may not be appropriate. For example, in the

case of killing the remains of harvest of the GMO, treatment of post harvest

remains by herbicide would not be a sufficient mechanism.



‘Equipment’ includes harvesters, seeders, storage equipment, transport

equipment (eg bags, containers, trucks), ginning facilities, clothing and tools.



‘Feral cotton’ means naturalised, self-perpetuating populations of

Gossypium hirsutum L. and/or Gossypium barbadense L.



‘GM’ means genetically modified.



‘GMO’ means Bollgard II® and Bollgard II®/Roundup Ready® cotton, which

are genetically modified cotton, as described at ‗GMO Description‘ at

Attachment B.



‘Isolation Zone’ means an area of land, extending outwards 50 metres in all

directions from the outer edge of a Location.



‘Location’ means an area of land where the GMO is planted and grown.



Note: In this licence, before the GMO is planted and grown in a field or other

area, this licence refers to that field or area as an area or place. Once the GMO

is planted in a field or place, while it is being grown and thereafter, this licence

refers to that field or place as a Location



APPENDIX 1

‘Material from Pollen Trap plants’ means seed, stubble, pollen or any GM

material (including parts of a plant) that is derived from or produced by

cotton from a Pollen Trap.



‘Material from the GMO’ means GM seed, stubble, pollen or any other GM

material (including part of GMO) that is derived from or produced by the

GMO, but does not include cotton lint derived from ginning.



Note: cotton lint derived from ginning is not intended to be controlled by any

licence conditions in this licence.



‘Natural Waterways’ means waterway other than irrigation channel, holding

dam or storage pond used to collect water runoff from irrigated areas.



‗NRA‘ means the National Registration Authority for Agricultural and

Veterinary Chemicals.



‘OGTR’ means the Office of the Gene Technology Regulator.



‘Pollen Trap’ means an area of land, extending at least 20 metres in all

directions from the outer edge of a Location, containing non-genetically

modified cotton that is grown in such a way as to reasonably promote a dense

and vigorous growth and flowering of the non-genetically modified cotton at

the same time as the GMO.



‘Pollen Trap plant’ means a cotton plant from a Pollen Trap.



‘Restricted Zone’ means north of latitude 22º South in NT, QLD and WA.



‘Volunteer plant’ means progeny of the GMO or of a Pollen Trap plant.









APPENDIX 1

PART 3

Conditions of licence

The licence holder and persons covered by this licence must comply with the

conditions of this licence.



SECTION 1 GENERAL CONDITIONS



Informing people of their obligations

1.1 The licence holder must inform each person covered by this licence of the

obligations imposed on them as a result of the conditions in this licence.

1.2 The licence holder must provide the Regulator, on the Regulator‘s

written request, a signed statement from each person covered by this

licence that the licence holder has informed the person of the conditions

of this licence that apply to that person.

1.3 It is a condition of a licence that the licence holder inform the Regulator

if the licence holder:

(a) becomes aware of additional information as to any risks to the

health and safety of people, or to the environment, associated

with the dealings authorised by the licence; or

(b) becomes aware of any contraventions of the licence by a person

covered by the licence; or

(c) becomes aware of any unintended effects of the dealings

authorised by the licence.

Material Changes in circumstances

2.1 The licence holder must immediately, by notice in writing, inform the

Regulator of:

(a) any relevant conviction of the licence holder occurring after the

commencement of this licence;

(b) any revocation or suspension of a licence or permit held by the

licence holder under a law of the Commonwealth, a State or a

foreign country, being a law relating to the health and safety of

people or the environment;

(c) any event or circumstances occurring after the commencement of

this licence that would affect the capacity of the holder of his

licence to meet the conditions in it.









APPENDIX 1

Remaining an Accredited organisation

3.1 The licence holder must, at all times, remain an accredited organisation

and comply with any conditions of accreditation set out in the Guidelines for

Accreditation of Organisations.

Changes to details

4.1 The licence holder must immediately notify the Regulator in writing if

any of the contact details of the Project Supervisor change.



SECTION 2 SPECIFIC CONDITIONS IN CONNECTION WITH ANY RELEASE OF THE GMO

IN AUSTRALIA SOUTH OF LATITUDE 22º SOUTH (OUTSIDE THE

RESTRICTED ZONE)

1. The licence holder and persons covered by this licence must not deal

with the GMO South of latitude 22 degrees South (i.e., outside the Restricted

Zone) except as expressly authorised or contemplated by this licence.



Note: The conditions in this Section 2 apply to both Bollgard II ® and Bollgard

II®/Roundup Ready® cotton.



Locations and size of Release

2. Dealings with the GMO may be conducted outside the Restricted Zone.

Research on gene flow and environmental impacts

3. The licence holder must, in consultation with the OGTR, survey farms

to seek to determine the incidence of Volunteer plants on those farms and

their management by farmers. The survey results must be included in the

written report that must be submitted to the Regulator within 90 days of each

anniversary of this licence.



Note: A condition below requires annual written reporting to the Regulator.

See below.



4. The licence holder must, in consultation with the OGTR, develop an

agreed research program to ensure the ongoing effectiveness of management

actions and to monitor the environmental impacts of the GMO. This must

include (but need not be limited to) collecting information on:

(a) the effects of the GMO on key pests and beneficial insects

(including Diptera) and soil microorganisms; and



APPENDIX 1

(b) the potential development of pests resistant to the insecticidal

activity of the GMO.

5. The licence holder must, in consultation with the OGTR, survey for

shifts in weed resistance associated with growing Bollgard II/Roundup Ready

cotton. The survey results must be included in the written report that must

be submitted to the Regulator within 90 days of each anniversary of this

licence.



Note: A condition below requires annual written reporting to the Regulator.

See below.



Testing methodology

6. The licence holder must provide a written instrument to the Regulator

describing an experimental method that is capable of reliably detecting the

presence of the GMO and any transferred genetically modified material that

might be present in a recipient organism. The instrument must be provided

within 30 days of planting of the GMO.

Reporting

7. The licence holder must provide the Regulator with a written report

within 90 days of each anniversary of this licence, in accordance with any

Guidelines issued by the Regulator in relation to annual reporting.



SECTION 3 SPECIFIC CONDITIONS IN CONNECTION WITH ANY RELEASE OF THE GMO

IN AUSTRALIA WITHIN THE RESTRICTED ZONE



GMO not permitted in Restricted Zone except as contemplated by this licence

8. The GMO and Material from the GMO are not permitted in the

Restricted Zone (including the growing of the GMO and the transportation of

the GMO into or within the Restricted Zone), except in accordance with the

following specific conditions.



Note: The conditions in this Section 3 apply to both Bollgard II and Bollgard

II/Roundup ready cotton.









APPENDIX 1

Locations and size of release

9. The GMO may be planted and grown at up to 20 (and not more than

20) Locations at any one point in time.



No single Location may exceed 200 hectares in area.



The maximum area of all Locations under all Licences planted to the GMO at

any one point in time must not exceed 800 hectares.

10. The GMO must not be planted within 50 metres of a Natural

Waterway.

Notification of Locations

11. Prior to planting the GMO at an area, the area‘s GPS coordinates and

either a street address or other directions to the area must be provided to the

Regulator by notice in writing. The notice must identify the GMO proposed

to be grown at the area, by reference to its ‗GMO details‘ as set out at

Attachment B. If more than one of the GMOs identified at ‗GMO details‘ at

Attachment B is proposed to be grown at the area, the notice must provide

additional details about where, within the area, the different GMOs are

proposed to be grown.

Licence holder must be able to access and control places used in connection with

this licence

12. The licence holder must be able to access and control a Location or

other area used in connection with this licence, other than areas where stock

are fed GM cotton seed and areas where stock fed with GM cotton seed are

subsequently grazed, to the extent necessary to comply with this licence, for

the duration of the life of the licence.

Notification of planting of the GMO

13. The licence holder must provide notices in writing to the Regulator in

respect of each of the following:

(a) the short term forecasted date or dates of commencement of

planting of the GMO at each area proposed to be planted (and

Pollen Trap in respect of each area, if any) ('the short term

forecast planting date notice'). This notice must be provided at

least 7 days, and not more than 20 days, prior to the forecasted

date or dates of commencement of planting set out in the notice;







APPENDIX 1

(b) the actual date or dates of commencement of planting of the

GMO at each Location (and Pollen Trap in respect of the

Location, if any) (‗the actual planting date notice‘). This notice

must be provided within 7 days of commencement of planting of

the GMO at the Location.

Notification of commencement of seed set of the GMO

14. The licence holder must provide notices in writing to the Regulator in

respect of each of the following:

(a) the short term forecasted date or dates of commencement of seed

set of the GMO at each Location (and Pollen Trap in respect of

each Location, if any) ('the short term forecast seed set date

notice'). This notice must be provided at least 7 days, and not

more than 20 days, prior to the forecasted date or dates of

commencement of seed set, as set out in the notice;

(b) the actual date or dates of commencement of seed set of the GMO

at each Location (and Pollen Trap in respect of each Location, if

any) ('the actual seed set date notice'). This notice must be

provided within 7 days of commencement of seed set of the GMO

at the Location.

Notification of commencement of harvest of GMO

15. The licence holder must provide notices in writing to the Regulator in

respect of each of the following:

(a) the short term forecasted date or dates of commencement of

harvesting of the GMO at each Location (and Pollen Trap in

respect of each Location, if any) ('the short term forecast harvest

date notice'). This notice must be provided at least 7 days, and

not more than 20 days, prior to the forecasted date or dates of

commencement of harvesting set out in the notice;

(b) the actual date or dates of commencement of harvesting of the

GMO at each Location (and Pollen Trap in respect of each

Location, if any) ('the actual harvest date notice'). This notice

must be provided within 7 days of commencement of harvesting

of the GMO at the Location.

Measures to manage gene flow – Locations must be surrounded by Pollen Traps or

Isolation Zones

16. Each Location must be surrounded by either:





APPENDIX 1

(a) a Pollen Trap; or

(b) an Isolation Zone.

Conditions relating to Pollen Traps

17. If a Pollen Trap is planted, the plants in it (Pollen Trap plants) must be

handled and controlled as if they are the GMO (ie subject to other applicable

conditions elsewhere in this licence), and Material from Pollen Trap plants

must be handled and controlled as if it is Material from the GMO (ie subject

to applicable conditions elsewhere in this licence).



18. A Pollen Trap must be able to be accessed and controlled by the licence

holder to an extent that is commensurate with the licence holder‘s rights to

access the Location within it.



Note: Specific conditions about Cleaning Pollen Traps occur elsewhere in this

licence.



Conditions relating to Isolation Zones

19. No cotton of any kind may be grown in an Isolation Zone while the

GMO is being grown at the Location within it.



20. An Isolation Zone must be able to be accessed and controlled by the

licence holder to an extent that is commensurate with the licence holder‘s

rights to access the Location within it.

Harvest and post-harvest procedures

21. If the GMO or Pollen Trap plants are harvested, they must be

harvested separately from any other cotton.



22. If seed cotton harvested from the GMO or from Pollen Trap plants is

ginned, it must be ginned separately from any other cotton.



23. Following ginning, seed from the GMO and Pollen Trap plants must be:

(a) stored in a sealed container that is signed so as to indicate that it

contains GM cotton seed, within a locked facility that is signed so

as to indicate that GM cotton seed is stored within the facility; or

(b) exported; or

(c) transported south of latitude 22º South; or







APPENDIX 1

(d) used for stockfeed in accordance with the conditions in this

licence (see below); or

(e) rendered non-viable by roller crushing or extrusion; or

(f) incinerated.



24. Any cotton seed obtained from harvest may only be transported to the

extent necessary to gin it and then to comply with this licence.



Note: This licence contains other conditions relating more specifically to

transportation of the GMO and GM Material from the GMO.



The GMO and GM Material may be used as animal feed

25. Following ginning, the GMO and Material from the GMO may be fed

to stock in the Restricted Zone only if feeding takes place inside stockyards,

feedlots or dairies.



26. The licence holder must inform:

(a) cotton gins and persons using the GM cotton seed as stockfeed, of

the licence conditions below relating to monitoring of areas where

stock are fed the GMO and destruction of Volunteer plants; and

(b) encourage recipients of ginned GM cotton seed to adopt the

conditions as farm practices.



27. If stock are fed the GMO or viable Material from the GMO, the licence

holder must take reasonable steps to monitor the following areas for the

existence of Volunteer plants:

(a) All areas where the stock were fed;

(b) All areas where stock were grazed within, 25 days of feeding the

GMO; and

(c) All areas where the stock were subsequently housed, within 25

days of feeding the GMO.



28. The Licence holder must ensure that monitoring is performed by a

person who is able to recognise Volunteer plants and that reasonable steps

are taken to destroy any Volunteer plants detected during monitoring before

they set seed.









APPENDIX 1

Cleaning – post harvest and generally

29. Where Equipment (including harvesters, storage equipment, transport

equipment, ginning facilities and clothing), a Location or other area is used

pursuant to this licence in respect of the GMO, viable Material from GMO,

Pollen Trap plants or viable Material from Pollen Trap plants, it must be

Cleaned.



30. For each Location, either within 14 days of harvest of the GMO or 9

months after planting, whichever occurs first, the Location must be Cleaned.



31. Within 14 days of either harvest or Cleaning of the GMO at a Location,

whichever occurs first, the area containing the Pollen Trap in respect of that

Location, if any, must be harvested or Cleaned.



32. When Equipment is Cleaned, the area in which the Equipment is

Cleaned must also be Cleaned.



Note: For the sake of clarity, it is not necessary for Equipment to be Cleaned

only at a Location.



33. Cleaning must occur immediately or as soon as practicable after the use

and before use for any other purpose.



Note: For example, if seed is harvested with a mechanical harvester, the

harvester must be Cleaned immediately following its use and before any other

cotton is harvested.





Post-harvest monitoring

34. Following Cleaning of each Location, the following places must be

monitored for the existence of Volunteer plants:

(a) the Location;

(b) the Pollen Trap in respect of the Location, if any;

(c) any areas used to Clean Equipment used in connection with the

GMO or to destroy the GMO, viable Material from the GMO,

Pollen Trap plants or viable Material from Pollen Trap plants;

and







APPENDIX 1

(d) irrigation channels or drains through which water used on the

Location (or Pollen Trap, if any) flows.



35. Monitoring must be performed by a person who is able to recognise

Volunteer plants.



36. Any Volunteer plants detected during monitoring must be Destroyed

before setting seed.



37. All the places required to be monitored must be monitored at least once

every 60 days for at least:

(a) 12 months from the last day of Cleaning of the Location; or

(b) if a cotton crop is planted at the Location in the growing season

following the growth of the GMO, until such time as the

subsequent cotton crop is planted.



Note: This licence allows a single crop of cotton (either GM cotton or non-GM

cotton) to be planted in the growing season after the GMO is grown, in certain,

limited situations (see the conditions in connection with this below). If a

second cotton crop is grown at a Location, the condition above requires

monitoring of the Location to take place between the time of Cleaning of the

GM cotton at the Location and the planting of the second cotton crop. After

harvest or Cleaning of the second cotton crop, monitoring of the Location must

then be recommenced for a further 12-month period.



38. The results of monitoring activities must be reported to the Regulator

in writing within 30 days of any day on which monitoring occurs. Reporting

must include:

(a) details of the areas monitored;

(b) details of the date of monitoring;

(c) the names of the person or persons who undertook the monitoring

and details of the experience, training or qualification that

enabled them to recognise Volunteer plants;

(d) the number of Volunteer plants observed, if any;

(e) details of whether the Volunteer plants observed, if any, occurred

in the Location, the Pollen trap, areas used to Clean Equipment

or in irrigation channels or drains;

(f) details of the development stages reached by the Volunteer plants,

if any;





APPENDIX 1

(g) details of methods used to Destroy Volunteer plants identified, if

any; and

(h) details of the date on which Volunteer plants were Destroyed.

General conditions on use of Locations post-harvest

39. These general conditions on the use of Locations post-harvest

immediately below are subject to special conditions in connection with

subsequent growing of a single cotton crop that appear below these general

conditions.



40. If the GMO is grown at a Location, no other cotton plant of any kind

may be grown at the Location, or Pollen Trap in respect of the Location, if

any, after harvest of the GMO or Pollen Trap plants, until monitoring

obligations are completed.



41. If the GMO is grown at a Location, no plants may be planted at the

Location, or Pollen Trap in respect of the Location, if any, until monitoring

obligations are completed unless:

(a) the plants are grasses (grass pastures), cereals (cereal crops); or

(b) the plants are plants agreed to in writing by the Regulator; and

(c) the Regulator is satisfied that monitoring and Destruction of

Volunteer plants prior to setting seed will not be adversely

affected by the planting.



Special conditions on use of Locations post-harvest - single crop of cotton may be

planted in growing season immediately following GMO growing season in limited

situations

42. If no cotton of any kind was grown at a Location in the 12 months

preceding the growing of the GMO at the Location, then a single crop of

non-GM cotton may be grown at the Location and pollen Trap in the growing

season immediately following the growing of the GMO. If non GM-cotton is

grown at the Location in the growing season immediately following the

growing of the GMO, the cotton shall be taken to be the GMO for the

purposes of this Licence.



Note: This means that the conditions in this licence, as they apply to the GMO

(for example as they apply to areas and Equipment used in connection with the

GMO) are applicable to the subsequent non-GM cotton crop. These conditions





APPENDIX 1

are intended to allow a single crop of non-GM cotton to be planted at a Location

in the growing season immediately following the season in which the GMO is

grown at the Location.



If a subsequent cotton crop is grown, the conditions above are intended to have

the effect that regardless of whether the subsequent cotton crop is actually GM

or not, that cotton must be regarded as, and handled and controlled under this

licence as if it is GM cotton. It is intended that the licence conditions in this

licence will be applicable to that subsequent cotton crop, regardless of the fact

that the cotton may not be GM. For example, where a second cotton crop is

grown, it is intended that the cotton will form part of the 800 hectare limit in

that period, that the cotton will be surrounded by an Isolation Zone or Pollen

Trap, that the Location will be harvested, Cleaned and monitored and the cotton

transported in accordance with the other licence conditions in this licence.



Transportation of the GMO, Material from the GMO, Pollen Trap plants and

Material from Pollen Trap plants

43. Subject to the other transport conditions below, the GMO, Material

from the GMO, Pollen Trap plants and Material from Pollen Trap plants

must not be transported within or into the Restricted Zone unless contained

within a primary, sealed container that is packed in a secondary, unbreakable

container.



Note: Cotton lint derived from ginning is not subject to these transportation

conditions.



44. Cotton modules may be used to transport the GMO, Material from the

GMO, Pollen Trap plants and Material from Pollen Trap plants, if they are

covered with a tarpaulin, then wrapped securely in shadecloth in such a way

as to prevent dissemination of cotton seed, and then placed inside a sealed

chain-bed truck.



45. Seeds from the GMO or Pollen Trap plants that have been ginned may

only be transported for export in sealed, airtight shipping containers.



46. Seeds from the GMO or Pollen Trap plants that have been ginned may

be transported to stockyards, feedlots or dairies (for use as stockfeed) in







APPENDIX 1

Covered Vehicles.



47. Every container used to transport the GMO, viable Material from the

GMO, Pollen Trap plants or viable Material from Pollen Trap plants must be

labeled:

(a) to indicate that it contains genetically modified cotton; and

(b) with telephone contact numbers for the licence holder and

instructions to contact the licence holder in the event that the

container is broken or misdirected.



48. The licence holder must have in place accounting procedures to verify

whether the same quantity of GMO, viable Material from the GMO, Pollen

Trap Plants or Material from Pollen Trap plants sent is delivered. Routes,

methods and procedures used for transportation of the GMO, viable Material

from the GMO, Pollen Trap plants and viable Material from Pollen Trap

plants must be documented.

Contingency Plan

49. Within 30 days of the date of the commencement of this licence, a

written Contingency Plan must be submitted to the Regulator detailing

measures to be taken in the event of the unintended presence of the GMO,

viable Material from the GMO, Pollen Trap plants or viable Material from

Pollen Trap plants outside a Location, Pollen trap in respect of a Location or

other area that must be monitored.



50. The Contingency Plan must include details of procedures to:

(a) ensure the Regulator is notified immediately if the licence holder

becomes aware of an event;

(b) destroy any of the GMO, viable Material from the GMO, Pollen

Trap plants or viable Material from Pollen Trap plants; and

(c) monitor for and destroy any Volunteer plants that may exist as a

result of the event.



51. The Contingency Plan must be implemented in the event that the

unintended presence of the GMO, viable Material from the GMO, Pollen

Trap plants or viable Material from Pollen Trap plants is discovered outside

an area that must be monitored.









APPENDIX 1

Compliance Management Plan

52. Prior to planting the GMO, a written Compliance Management Plan

must be provided to the Regulator. The Compliance Management Plan must

describe in detail how the licence holder intends to ensure compliance with

these conditions and to document that compliance.

Research on gene flow and environmental impacts

53. The licence holder must, in consultation with the OGTR, conduct an

annual survey of:

(a) areas where the stock were fed; and

(b) areas where stock subsequently moved after feeding;



in order to seek to determine the prevalence of Volunteer plants in these

places. The survey results must be included in the written report that must

be submitted to the Regulator within 90 days of each anniversary of this

licence.



Note: A condition below requires annual written reporting to the Regulator.

See below.



54. The licence holder must, in consultation with the OGTR, develop an

agreed research program to ensure the ongoing effectiveness of management

actions and to monitor the environmental impacts of the GMO. This must

include (but need not be limited to) collecting information on:

(a) the potential weediness of GM cotton in northern Australia;

(b) the effects of the GMO on key pests and beneficial insects

(including Diptera) and soil microorganisms; and

(c) the potential development of pests resistant to the insecticidal

activity of the GMO.



The licence holder is encouraged to conduct research in connection with the

following:



(a) the distribution of Feral cotton in northern Australia;

(b) the effectiveness of any Pollen Trap in preventing gene flow from

the GMO to non-genetically modified cotton, including Feral

cotton;









APPENDIX 1

(c) the effectiveness of any Isolation Zone in preventing gene flow

from the GMO to non-genetically modified cotton, including

Feral cotton;

(d) the relative prevalence and impact on Feral cotton in northern

Australia of lepidopteran and other arthropod herbivores;

(e) integrated pest management strategies for Bollgard II® and

Bollgard II®/Roundup Ready® cotton, particularly in northern

Australia;

(f) the distribution of volunteer cotton along transport routes in

northern Australia.



Note: The OGTR will review licence conditions, including approval for further

planting, in light of results from the research program after two years from the

date of issue of this licence (or earlier).





Testing methodology

55. The licence holder must provide a written instrument to the Regulator

describing an experimental method that is capable of reliably detecting the

presence of the GMO and any transferred genetically modified material that

might be present in a recipient organism. The instrument must be provided

within 30 days of planting of the GMO.

Reporting

56. The licence holder must provide the Regulator with a written report

within 90 days of each anniversary of this licence, in accordance with any

Guidelines issued by the Regulator in relation to annual reporting.









APPENDIX 1

APPENDIX 2 REASONS FOR LICENCE CONDITIONS



The reasons for inclusion of the specific licence conditions follow (with

reference to the numbering of the conditions in the licence). The object of

most of the conditions is to limit the potential for spread and persistence of

the GM cotton in the environment outside release sites in the Restricted Zone

(north of latitude 22South in QLD, NT and WA) in order to reduce the

potential for risks to the environment.



Note: Conditions 8 to 56 only apply to releases north of latitude 22South.

Conditions 1, 2 and 9 to 11 Location and size of release

These conditions limit the dealings that may be undertaken by the licence

holder to those that have been sought by the licence holder and considered

and assessed by the Regulator. The Regulator requires detailed information

about release site locations north of latitude 22South prior to

commencement of any release in order to maintain the GMO Record, as well

as to plan OGTR monitoring of the Locations. Dealings other than those

authorised in the licence are prohibited and would require separate

assessment by the Regulator.

Conditions 3 to 5, 53 and 54 Research

This condition requires information to be collected and provided to the

Regulator on the environmental impact of the GM cotton and on the efficacy

of management actions, to assist the Regulator in future risk assessments.

Research must be planned in consultation with the OGTR, so that the OGTR

can assess whether any study is sufficiently rigorous to provide meaningful

data. The research will include an annual survey of the prevalence of GM

cotton volunteers associated with the use of using GM cotton seed as a

stockfeed in northern Australia.



Conditions 6 and 55 Testing methodology

This condition addresses the risk of persistence or dissemination of the GM

cotton or its genetic material in the environment going unidentified. The

condition requires the licence holder to provide a method that will detect the

GM cotton or its genetic material, for example if transferred to other cotton

plants. The ability to test for the presence or absence of the GMO or its

genetic material will enable the Regulator to be satisfied that risks are being





APPENDIX 3

adequately managed, or alternatively that additional measures may need to

be imposed to manage risks.

Conditions 7 and 56 Reporting

This condition requires the licence holder to provide an annual report to the

Regulator, thereby satisfying the Regulator that risks are being adequately

managed by the licence holder.

Condition 8 Dealings in the Restricted Zone

This condition requires further specific conditions to apply if the GMO or

viable material from the GMO is grown or transported north of latitude

22South in QLD, NT or WA (the Restricted Zone).



Conditions 9 to 11

See ‗Conditions 1, 2 and 9 to 11‘ above.



Condition 12 Access to Locations

This condition requires that the licence holder or persons covered by this

licence have access to any Location, Pollen Trap, Isolation zone or other area

used in connection with this licence, to enable them to comply with other

conditions of this licence.



Condition 13 to 15 Notification of the planting, seed set and harvesting of the

GMO

This condition ensures that minimum advance information about the likely

higher risk times of releases north of latitude 22South (namely the likely

planting, flowering and harvest dates) is provided to the OGTR to ensure

proper planning of the OGTR monitoring of the Locations. The OGTR

monitoring strategy is an integral component of the overall management

strategy for the proposed release north of latitude 22South. The Regulator

considers that these conditions are necessary and adequate to ensure

management of the risks posed north of latitude 22South at the higher risk

times of planting, flowering and harvest.









APPENDIX 3

Conditions 16 to 20 Measures to manage gene flow (Pollen Traps, Isolation

Zones)

These conditions are intended to minimise the risk of gene flow from the GM

cotton to other cotton crops or to feral cotton north of latitude 22South by

physical separation. Pollen Traps and Isolation Zones serve as barriers to

pollen movement, by either wind or insect pollinators, to plants outside the

Location. Access to the these areas by the licence holder or persons covered

by the licence ensures the licence holder can continue to meet the obligations

of the licence. The Regulator considers that these conditions are necessary

and adequate to manage the risk of gene flow north of latitude 22South.

Conditions 21 to 24 Harvest and post-harvest procedures

These conditions seek to reduce the risk of the GMO being disseminated or

persisting in the environment north of latitude 22 South through spillage or

contamination of seed at harvest or during ginning. The Regulator considers

that these conditions are necessary to adequately minimise this risk.

Harvesting and ginning of the GM cotton separately from any other cotton

reduces the risk of contamination of other crops. Immediate storage of seed

in sealed, clearly labelled containers, export of seed or destruction of seed

after ginning, coupled with minimum transportation of the GMO, is

necessary to ensure minimum risk of the GMO disseminating into the

environment.

Conditions 25 to 28 Use as animal feed

These conditions are to limit, monitor for and control the spread of the GMO

as a result of its use as animal feed north of latitude 22South. The

Regulator considers that these conditions are necessary and adequate to

manage the risks of persistence and dissemination of the GMO north of

latitude 22South as a result of its use as animal feed.

Conditions 29 to 33 Cleaning – post harvest and generally

These conditions describe the cleaning requirements for equipment,

Locations and other areas used in releases north of latitude 22 South, in

order to minimise the risk of spread or persistence of the GMO outside the

release site.



Cleaning of equipment used in connection with the GM cotton is considered

necessary to adequately minimise the risk of dissemination of the GMO, for







APPENDIX 3

example through the spread of GM cotton seeds during subsequent use of

equipment.



Cleaning of the Location and Pollen Trap ensures that the GM cotton and

material from the GM cotton are destroyed, and is considered necessary to

adequately minimise the risk of the GMO persisting at or spreading beyond

the Location.



Cleaning as soon as possible after the specified activity is considered

necessary to further reduce the risk of dissemination and persistence of the

GMO.

Conditions 34 to 38 Monitoring – post harvest and generally

These conditions are considered necessary to minimise the risks of persistence

and dissemination of the GMO or its genetic material after harvest for

releases north of latitude 22 South arising from volunteer cotton germinating

and setting seed. These conditions are also considered necessary because the

Cleaning conditions are not considered to be sufficient, of themselves, to

adequately manage these risks.



The Regulator considers that regular monitoring north of latitude 22 South,

and destruction of volunteer cotton prior to seed set on the release site, any

areas used for cleaning equipment, and irrigation channels and drains

through which water used at the release site flows is necessary and adequate

to minimise and manage the risks.



The Regulator considers that requiring monitoring for at least 1 year is

necessary and adequate to manage the risks of persistence and dissemination

of the GMO or it genetic material. If a Location is to be used in the

subsequent (second) season for growth of GM cotton, monitoring for 6

months between harvest and replanting is considered adequate to manage the

risks, as the Location will be subject to monitoring after the second season

harvest.



These conditions require regular reporting to the Regulator of the results of

monitoring activities, including the incidence, developmental stage and

methods of destruction of volunteers. This condition is considered necessary

to demonstrate that the licence holder is managing the risks and complying

with the conditions of the licence, and to identify whether any additional





APPENDIX 3

management actions might need to be implemented to adequately manage the

risks.

Conditions 39 to 42 Use of Locations post-harvest

These conditions are considered necessary and adequate to manage the risk of

persistence or dissemination of the GM cotton or its genetic material in the

environment north of latitude 22 South during subsequent use of the

Location.



The condition permitting the planting of cereals and grass pastures is

considered compatible with management of the risks of persistence and

dissemination of the GMO because these crops enable the ready identification

and destruction of volunteer cotton. The growth habits of these crops also

suppress the development of volunteer cotton seedlings. The planting of

these crops is considered to reduce the risk of persistence or dissemination of

the GM cotton.



The condition requiring approval in writing from the Regulator for the

planting of alternative crops is considered necessary to manage the risks of

persistence and dissemination of the GMO, allowing the Regulator to assess

whether the risks posed by the emergence of volunteer cotton can be

adequately addressed through the ready identified and destruction of

volunteer cotton in those crops.



The condition permitting the growing of non-GM cotton (to be treated as if it

were GM cotton) at a Location in the season following the GM cotton crop is

considered compatible with management of the risks of persistence and

dissemination of the GMO. Any GMO persisting after the first season will

continue to be dealt with in accordance with OGTR licence conditions,

including monitoring following harvest of the second season crop.



Conditions 43 to 48 Transport

This condition addresses the risks of persistence or dissemination of the GM

cotton in the environment north of latitude 22 South posed by transport,

especially unintentional release through spillage or contamination. These

risks are managed by this condition by requiring all material from GM cotton

to be double contained during transport north of latitude 22 South, that it be







APPENDIX 3

clearly labelled, and that all viable material from GM cotton is accounted for

during transport.

Conditions 49 to 51 Contingency plans

This condition addresses the risks posed through unintended presence of the

GMO or its genetic material north of latitude 22 South. The condition

requires the licence holder to supply the Regulator with a documented

contingency plan that will be enacted in the event of unintended presence of

the GMO north of latitude 22 South. This will enable the Regulator to be

satisfied that the licence holder will be able to implement measures which will

effectively manage any risks posed by such an eventuality. It also enables the

Regulator to revise the contingency plan or impose licence conditions to

require any other measures that might be necessary to prevent the continued

spread or persistence of the GMO. This condition also requires that the plan

must have procedures to ensure immediate notification of the Regulator in the

event of an unintentional presence of the GMO north of latitude 22 South, so

that the Regulator can take any actions necessary to protect the environment.

Condition 52 Compliance Management Plan

This condition requires the licence holder to document to the Regulator that

they can comply with the conditions of the licence. This enables the

Regulator to be satisfied that the risks posed by the dealing with the GMO

can be adequately managed through compliance with the management

measures set out in the licence.

Conditions 53 and 54

See ‗Conditions 3 to 5, 53 and 54‘ above.

Condition 55

See ‗Conditions 6 and 55‘ above.

Condition 56

See ‗Conditions 7 and 56‘ above.









APPENDIX 3

APPENDIX 3

APPENDIX 3 PUBLIC SUBMISSIONS ON THE APPLICATION AND RISK

ASSESSMENT AND RISK MANAGEMENT PLAN



a

Submission from: A: agricultural organisation; I: individual; E: environmental organisation;

F: food interest organisation; C: consumer/public interest organisation

b

Refers to Ch (Chapter), OSA: outside scope of the assessment; NR: No specific response; CBD:

‗The Biology and Ecology of Cotton (Gossypium hirsutum) in Australia‘, available online at

www.ogtr.gov.au.

Sub. Consideration

TypeA

No:

Summary of issues raised of issueB

1 I  …summary documentation…by GTR…makes a Ch 9

convincing case to reject application…for extension to

area…where GM cotton can be grown

 …applicant provides no scientific evidence that earlier Ch2 & Ch 6

decision…was wrong…[to] restrict the release GM

cotton be restricted to areas south of 22º South

 ….Act requires that decisions by Regulator be based on Noted

scientific evidence…if at all possible by scientific

experimentation

 …application based on opinions not experimentation Noted

 ….Regulator should require that experiments be done Ch 9 &

to test implied assertion of the applicant that earlier Appendix 1

restrictions were incorrect

 …should not approve the application until those Noted

experiments have been done and assessed.

 [Gives examples of types of experiments that needs to Ch 9 &

be conducted] Appendix 1

2 I  ….genetically modified products and inventions…are Ch 5

not good for humans…like any other artificial things

that come in the market they all poses health hazards

to human life

 …fact of life natural products are best…why…include Ch 5

artificial things if …ruin our health

 …profit motivates those who invent this artificial OSA

resources…[which] only create more problems…it

contradicts nature which is the source we produce and

consume. We drawn our resources from it as a means

of our existence so why replace it with flawed

alternatives.









APPENDIX 3

 …genetically modified technology not good to be Ch 5

released commercially, nor…large scale trial…once

released…bad effects that it could cause can never be

remedied…why…try something…not good for

humans…what benefits…if we are already exposed…to

the negative effects this genetically modified products

brought in our lives

 …only people…benefits…GM products are those who OSA

created them through wealth from profits….at the

expense of all human beings.

 different kinds of illnesses existed…might be caused Ch 5

through the GM products …people are consuming

without their knowledge.

 …because of the profit motives as the driving force of OSA, Ch 5

GM technology founders they will do anything to

succeed at the expense of the peoples health just to

acquire wealth.

 …final decision depends on the intuition of the OSA

regulators of this technology, whether they will listen to

their conscience or to the wealth it will create for them.

3 A  …vehemently protest planting of GM cotton (in fact Noted

ANY GM CROP) in Katherine (or ANYWHERE in

Australia)

 …well known that Monsanto…DISMAL record of Ch 9

controlling trialed releases of GM crops.

 …no one can control GM organisms…they will Ch 7

contaminate not only organic farms but conventional CBD

farms

 …Monsanto original makers of DDT and Agent OSA

Orange and of course glyphosate containing herbicide

– still promoted as safe, soft option (despite the state of

New York successfully suing Monsanto for its claim

that glyphosate containing herbicide is both

bio-degradable and safe).

 …cotton is a notorious user of precious water, OSA

Insecticides, and Herbicides (glyphosate).

 …The Pope has just spoken out against GM, so has the Noted

Prince of Wales – many scientists (not lined to

Bio-Tech companies) have also spoken out against GM.

4 I [NB: this is an independent submission, despite its Noted

similarity to that of similar submissions including, for

example, submission no. 3]



 vehemently protest planting of GM cotton (in fact ANY

GM CROP) in Katherine (or ANYWHERE in

Australia)

 …well known that Monsanto…DISMAL record of Ch 9

controlling trialed releases of GM crops.







APPENDIX 3

 …no one can control GM organisms…they will Ch 7

contaminate not only organic farms but conventional CBD

farms

 …Monsanto original makers of DDT and Agent OSA

Orange and of course glyphosate containing herbicide

– still promoted as safe, soft option (despite the state of

New York successfully suing Monsanto for its claim

that glyphosate containing herbicide is both

bio-degradable and safe).

 …cotton is a notorious user of precious water, OSA

Insecticides, and Herbicides (glyphosate).

 …The Pope has just spoken out against GM, so has the Noted

Prince of Wales – many scientists (not lined to

Bio-Tech companies) have also spoken out against GM.

5 I  …strongly oppose use of GM seeds Noted

 …no controlling of a live form that can be blown miles Ch 7 & CBD

by the wind, carried by birds, moved on car tyres – to

cross pollinate other crops and natives

 …support [letter from Bio-Dynamic (Farming & Noted

Gardening) Network NT sub No. 3 above]

6 I  …apposed to Monsanto‘s application to release Noted

genetically modified cotton in the region of Katherine

(NT)

 ...Monsanto has not yet made their case Noted

 …recent New Zealand elections shows how uneasy OSA

many people feel about the rush to adopt GM

technology…Australia would be well advised to take

the careful NZ approach which will make our clean

green produce more and more desirable overseas

7 I [NB: this is an independent submission, despite its Noted

similarity to that of similar submissions including, for

example, submission no. 3]



 …our bodies are designed to be healthy, and heal

themselves in the natural environment that was created

for them – DON‘T MESS WITH IT – IT MAKES US

SICK!!!!

 …vehemently protest planting of GM cotton (in fact Noted

ANY GM CROP) in Katherine (or ANYWHERE in

Australia)

 …well known that Monsanto…DISMAL record of Noted

controlling trialed releases of GM crops.

 …no one can control GM organisms…they will Ch 7

contaminate not only organic farms but conventional CBD

farms









APPENDIX 3

 …Monsanto original makers of DDT and Agent OSA

Orange and of course glyphosate containing herbicide

– still promoted as safe, soft option (despite the state of

New York successfully suing Monsanto for its claim

that glyphosate containing herbicide is both

bio-degradable and safe).

 …cotton is a notorious user of precious water, OSA

Insecticides, and Herbicides (glyphosate).

 …The Pope has just spoken out against GM, so has the Noted

Prince of Wales – many scientists (not lined to

Bio-Tech companies) have also spoken out against GM.

 …would you grow this crop in your backyard and let OSA

your children play in it

8 A  no issues with the information supplied…supportive of Noted

the introduction of Bollgard II

 …positive contribution Ingard and Bollgard II have Noted

and will make to integrated pest management…this too

is part of risk assessment in the environment.

 …our experience in the field has seen a significant Noted

reduction in pesticide use through use of

Ingard…expecting Bollgard II to provide even greater

benefits

 …has allowed improved use of natural predatory Noted

insects or ‗beneficials‘ which have greatly

enhanced…ability to operate more robust and stable

integrated pest management systems at a commercial

level

 ...from a human safety prospective…technologies like Noted

Bollgard II further reduce chemical application

activities

9 I  …dismay and disapproval…Monsanto...getting Noted

permission to release GM crops anywhere in Australia.

 Australia has opportunity…world leader of clean, OSA

chemical free products…up to us to seize this chance as

more and more of worlds people are demanding this

type of product…to present ourselves this way…the

integrity has to be there and organics and other

products must be GM free

 …if we allow GM products …don‘t know what we are Ch 5, Ch 6

doing to nature nor the whole cycle Ch 7, Ch 8

CBD

 …Public Health, the environment and the security of Ch 5, Ch 6

our farmers…is at stake Ch 7, Ch 8

CBD

10 I  …express concern regarding the release of genetically Noted

modified seed in the Katherine region (and elsewhere

in Australia)… not to approved







APPENDIX 3

 …feel that genetic modification of our food is strongly Ch 5

contributing to the constantly rising rate of cancers

and other serious health issues experienced by Western

Cultures…isolated communities who do not have

access to GM foods, pesticides etc and who have clean

and uncontaminated water live healthy lives well into

their 90‘s and even their 100‘s

 …wish for myself, my children, their children etc to Ch 5, Ch 6

live long and healthy lives in a healthy environment Ch 7, Ch 8

which will not be possible if we continue to CBD

contaminate our bodies and our planet with artificial

toxins

 ...how will GM seed be prevented from contaminating Ch 7

other crops in region…would be an impossibility

 …ecosystem of the Northern Territory cannot sustain OSA

an activity such as cotton growing…waterways, native

flora and fauna will be destroyed and then what?

 Murray/Darling river systems and surrounding OSA

countryside has been decimated by cotton

growing…we don‘t want a repeat here

11 I  …register opposition to the general release of Bollgard Noted

II cotton as set out in the licence application

 The recent report (J. App. Ecol. vol. 39)…of Ch 7

cross-breeding of weeds from genetically modified

sugar beet crops underlies the likelihood of

unanticipated and irreversible changes which can

follow such releases

 …similar results from Ohio State University relating to Noted

sunflowers support the view that much more

investigation should proceed general release

12 C  …express opposition to the possible production of Noted

[GM] cotton in the Katherine/Daly areas

 …effects on water quality through land clearing, OSA

chemical runoff and water usage…will impact on both

Katherine and Daly Rivers

 …no environmental impact study has been done and Noted, but see

the time frame for the licence to be granted has grave Chapters 5-8

concerns for local and tourists that wish to use these

rivers

13 C  …lodge our objections to the Monsanto Noted

application…for commercial release of BT II or

Bollgard II / Roundup Ready cotton

 …insufficient research has been completed for Chapters 5-7

conditions associated with agriculture for cotton in

tropical Australia…premature approval of genetically

modified varieties is not the way to overcome the array

of unique idiosyncrasies surrounding the Top End of

Australia





APPENDIX 3

 …recognise that tropical savannahs are most OSA

depauperate and least understood landscapes…soils

are severely leached nutrients and require huge input

to achieve even modest gains…this fact alone raises

serious alarm bells for us on ecological grounds

 …most of the research with the BT 2 variety has OSA

probably carried out under conditions associated with

the traditional areas in the southern states…aware of

small trial plots in Katherine…however this is not

considered to be adequate in our view in the

understanding of the long term ecological and

economic viability of large scale broad-acre

agribusiness in tropical savannah regions

 …insufficient information and understanding is not the Noted

way forward…cost of reparation verses cost of

protection…is estimated to be at least 100:1

 …concerned that the trial assessing the economic and OSA

environmental sustainability, being undertaken…is not

completed

 researchers…have found that…toxin from BT corn is Ch 5

released into the soil from roots….scientists say more

research is needed to determine whether this exuded

toxin has a good, bad, or neutral effect organisms in

soil…situation where beneficial insects, and all insects

have a place in the environment, are at a possible

threat, because of excessive build up of these toxins

through genetic engineering, then the research and

understanding is not at a level where community

confidence will support continuation or further release

of genetically modified organisms

 Broad acre agriculture of any crop does irreparable OSA

damage to the environment…we have grave

reservations about the environmental consequences

from large scale farming as a whole in tropical

savannah areas

 …release of BT2 may be the only trigger needed for OSA

wholesale clearing of woodlands and subsequent water

harvesting on a massive scale that will…lead

to…environmental disaster for the rivers in question

[and gives examples]

 …what will happen to rivers when demand by OSA

irrigators for water is high and river level is

low…examples of rivers in NSW and central southern

Queensland… we call on you to look at the big picture

in your assessment









APPENDIX 3

 …although the press statements and reports regarding Ch 2, Ch 6 &

‗super weeds‘ may be emotive and selective CBD,

quoting…there may be some truth to these Ch 7

reports…the reports are yet to be scientifically

reviewed by peers of the authors…whether the findings

are confirmed or over turned is yet to be determined

however their research or preliminary results should at

least raise the issue and draw the attention to the

possibility of this gene transfer occurring. We must

wait further analysis and research

 …view of the Amateur Fishermen‘s Association of the Noted

Northern Territory is one of precaution…must not let

the destruction of rivers occur through water

harvesting, wholesale land clearing and ecological

modification by short term unsustainable agribusiness

14 I  …cotton grower… past 15 years…have been growing Noted

GMO cotton crops (Ingard) [since 1996]…found the

crop…extremely beneficial…in terms of profitability,

yield, input costs and sustainability

 …during this time [since 1996] …experienced a Noted

reduction in chemical usage…Bollgard II with its

enhanced efficacy on Heliothis will be of even greater

benefit to my farming operations…and… the

environment

 …support the release Bollgard II…[it] will minimise Noted

the risk of developing populations of resistant insects,

due to the presence of two, rather than one, insecticide

genes.

15 A  …major concern is risk of cotton becoming a weed in Chapter 6-9 &

northern areas of Australia…especially as application CBD

has stated that the risk of gene transfer from GM

cotton to cultivated cotton is high

 …outcrossing…would become a more serious problem Ch 7

were the plant become a weed

 …taking this into consideration, Monsanto should not Noted

be permitted to use testing sites in northern Australia

for two reasons…

 …firstly if there is a risk of the GM cotton becoming a Ch 6 & CBD,

weed during the trial phase one doesn‘t believe the Ch 9

plant should be used in a commercial capacity in this

area and thus there is no reason to undertake trials

here…

 …secondly, Monsanto have access to sites in southern Noted

Australia for trials where there is minimal risk of the

plant becoming a weed

16 I  …concerns that the size of the release cannot be Ch 6 & CBD

justified because of unaddressed issues such as

‗weediness in Northern Australia‘







APPENDIX 3

 …lack of acknowledgment in the application about Ch 5

possible future risks…needs to be a requirement for Ch 6 & CBD

periodic risk assessments to enable identification of Ch 7, Ch 8

unforseen health and environment outcomes

 …regulators should insist on the presence of traceable Appendix 1

genetic markers for tracking the transmission of such

release

 …the possible transfer to livestock or plants for human Ch 7

consumption is poorly addressed…more limited and

controlled releases needed

 …potential long term impacts on natural biodiversity Ch 6 & CBD

are not addressed adequately in the

application…possible setup of ‗seed banks‘ by

commercial release applicants to preserve natural

biodiversities

 …weediness of GM cotton in Northern Australia has Ch 6 & CBD

not been addressed in this application…factors such as

‗water availability‘ as stated by the applicant should be

tested in these environments

 …commercial releases should be first approved in the Noted

applicant‘s country…Australia should not be a ‗testing

ground‘ for future commercial releases of GM crops

 …data for risk assessments completed needs to be Noted

peer-reviewed, weight of evidence material and not

selectively chosen or referenced…use of testing

organisations such as CSIRO by applicants which

would raise credibility and easier licence approvals

17 C  …Monsanto have been in some of the world‘s worst Noted

environmental and human catastrophes…no respect

for the safety of humans and the environment

 …concerns about human health after exposure to Ch 5

Roundup

 …risk of genetic transfer with native relative Ch 7

species…concerns about the ‗Terminator technology‘

with the suicide mechanism which would allow

Monsanto the ability to self destruct great tracts of

plants worldwide

 …environmental uncertainty due to insufficient data Noted

available for our tropical woodlands of Northern

Australia

 …concerns about insect resistance, particularly from Ch 5

pesticides released through the roots into the

soil…effects of pollen etc on the health of insects such

as bees and butterflies

 …lack of clear, unbiased non profit based data to the Noted

community and govts.









APPENDIX 3

 …risk that natural varieties will become lost or OSA

replaced of natural diversity, by engineered varieties of

low or zero diversity

18 I  …environmental concerns regarding consequences of OSA

farming cotton and the effects it will have in the

Katherine region such as degradation of rivers and

wetlands, rising salinity etc

 …uncertainties associated with the issues of Ch 7 & CBD

cross-pollination and pest resistance in Bollgard II

Cotton

 …undeniable evidence in other parts of the world that Ch 5

these types of proposals can lead to irreversible Ch 6 & CBD

damage Ch 7, Ch 8

19 I  …should be a referendum ‗Does Australia want GM Noted

farming‘…claims serious risks ahead, now and in the

future for no apparent benefit

20 I  …any technology or system that can help address the OSA

cost/price squeeze, urbanisation, reducing the number

of chemicals used & pressure from the environmental

sectors in the community would be considered a

desirable benefit

21 I  …advantages such as reduced spraying from 16 down OSA

to 2 or 3, less chance of chemical drift onto houses and

neighbourhoods, increased profitability

22 C  …concerns about the potential weediness problem as Ch 6 & CBD

there has not been enough research carried out

 …risk of contamination [by Bt] during the wet season Ch 5, Ch 7

threatening aquatic life

 …believe strong public opinion should also be Noted

considered as well as scientific evidence

23 I  …concerns about farms being able to claim GM free OSA

status

 …concerns that the GM crop will generate resistance Ch 7

to natural bacteria and that this will transfer to natural

vegetation

 …contamination by a herbicide resistant crop will lead Noted

to more extreme herbicides to stop the

spreading…neighbouring fields will not be able to

claim the clean healthy label, leading to a loss of

exports

 …health concerns that 450 scientists signed a letter Ch 5

saying the risks of GE foods were acute toxic shock,

delayed immunological reactions, auto-immune

reactions, cancer and the spread of antibiotic resistant

bacteria

 …concerns about the trustworthiness of Monsanto Noted

with a bad track record







APPENDIX 3

24 E  …highlighted the Regulator‘s concerns about her Noted

inability to conclusively exclude a significant risk to the

environment north of 22 degrees South

 …lack of supporting evidence as not all approved Noted

limited and controlled release trials were carried out

 …insect resistance would occur sooner rather than Ch 8 & Ch 9

later unless preventative measures were carried out

 …overall lack of information for the regulator to Noted

approve this application

25 I  …concerns regarding soil micro organisms Ch 5, Ch 7,

Appendix 1

 …claims of contradictions in the RARMP Noted

26 I  …will see a decrease of reliance on the conventional Noted

methods of insect control…no downside to this

technology

27 I  …reduce insecticide use; reduce contamination of Noted

towns, river etc; allow areas previously unable to grow

cotton to be able to grow it; increase productivity; and

allow for a more competitive industry

28 I  …reduction of insecticides allowing it to be planted in Noted

environmentaly sensitive areas

29 E  …claims there is insufficient evidence to conclusively Ch 5

show that there is any risk to human health and the

environment…some information provided by

Monsanto has been biased and not available to the

public

 …only small areas of trials in Northern Australia Noted

providing limited results

 …no commercial releases worldwide for Bollard II OSA

have been approved and inference that it is ‗safe‘

because no application for commercial release has been

refused is misleading

 …no long-term work has been done on the long-term Ch 6 & CBD

impacts on native species Ch 7

 [quoted the Regulator]… that ‗based on available Noted

evidence it has not been possible to determine

conclusively that the proposed release would not pose

an environmental risk‘

 …concerns regarding the implications of extending the Noted, but see

area of application from 22 degrees to 20 degrees Ch 2, Ch 6 &

Ch 9

 …inadequate buffer zones and concerns regarding Ch 6 & CBD

contamination, particularly due to the wet season Ch 7

 …insect resistance would occur sooner rather than Ch 9

later, particularly insects in the soil as a result of

continued exposure









APPENDIX 3

 …the applicant should be required to collect data, Ch 9 &

prior to the commercial release as this information is Appendix 1

crucial to assessing the risks…data should be collected

on potential for weediness and non-target species and

should also include marsupial, mammals, fish etc

30 E  …concerns regarding the extension of the area to 20 Ch 1, Ch 6

degrees south

 …protection from the natural predator will increase Ch 6 & CBD

weediness and survival rate in wild conditions

 …against the use of unpublished data and considers Noted

data that isn‘t open to peer review or public scrutiny as

suspect

 …Gene Technology Act 2000 is fundamentally flawed Noted

 …dispersal and spread of seeds by natural processes, Ch 6 & CBD

particularly waterways Ch 7

 …status of QLD in relation to the release of GMOs Noted

31 C  …opposes the release prior to the conclusion of the Noted

research program currently underway in Katherine by

the NT Govt., CSIRO and the Australian Cotton

CRC…expansion of trials to incorporate issues such as

weediness, effects of hybridisation on natives &

impacts on the environment and human health

 …overuse of herbicides such as glyphosate and effects Ch 5

on ground water, soil and watercourses

 …concern about the lack of publicly available Noted

information

 …lack of field trials on potential weediness of cotton in Ch 6 & CBD

Northern Australia …further trials relating to

invasiveness much also be conducted

 …concern about the potential to hybridise with native Ch 7

Gossypium species which may create another weed

species or loss of biodiversity

 …inadequate buffer zones, particularly for the high CBD

rainfall north

32 E  …potential creation of ‗superweeds‘ in the NT Ch 6 & CBD

 …significant increased levels of Roundup into the Ch 2, Ch 5,

environment Ch 6 & CBD

 …potential negative impacts on people, native insects, Ch 6 & CBD

flora and fauna Ch 5, Ch 7

 …no identification of specific NT locations, nor the size Ch 9, Appendix 1

of the proposed GM crops

 …water issues: allocation, monitoring and lack of flood OSA

mitigation

 …lack of public consultation Noted

33 A  …enhancing sustainability of small rural areas that Noted

rely on the cotton industry to provide employment









APPENDIX 3

 …reduction in chemical usage…thus a reduction in Noted

production costs without affecting employment

34 I [NB: this is an independent submission, despite its OSA

similarity to that of similar submissions including, for

example, submission no. 33]



 enhancing sustainability of small rural areas that rely

on the cotton industry to provide employment

 …reduction in chemical usage…thus a reduction in Noted

production costs without affecting employment

35 C  …effects of water quality through land clearing, OSA

chemical runoff and high levels of water usage will

impact both Katherine and Daly Rivers

 …no environmental impact study has been OSA

done…grave concerns for locals and tourists who wish

to use these rivers

36 A  …supports on-going commitment to the development Noted

of appropriate resistance management plans

 …supports management plans tailored for local areas Noted

 …assumed difference of generations of insect pests Ch 9

does not appear to take into account the proposed

winter cropping for cotton, which would mean the

number of generations exposed would be similar to

that in the southern regions...therefore on its own does

not justify a different resistance management plan

37 A  …cotton CRC is committed to R&D…ensures the Noted

ongoing sustainability of Australian cotton

industry…other than this we have no vested interested

in the proposal

 …cotton CRC is supportive of the approval for Noted

commercial use of Bollard II cotton particularly in

existing cotton areas

 …Bollard II varieties hove demonstrated higher Noted

efficacy than INGARD and so offer sound prospects

for further reductions in pesticide applications

 …with two independent insecticidal proteins…greatly Noted

reduce the risk of Bt resistance evolving in key target

pests…maximise environmental benefits

 …INGARD cottons…important part of Integrated Pest Noted

Management (IPM) systems being widely adopted in

cotton industry…data shows higher abundance of

beneficial insects in INGARD cotton...these play a key

role in IPM…Bollard II will… be compatible with IPM

systems

 …we accept that all human health and environmental Noted

risks have been addressed in the RARMP…note that

no impediments for commercial release in southern

production regions are identified.





APPENDIX 3

 …regard to…areas north of 22º South…no particular Noted

position on commercial release…accept that only

…Ord river region of WA could contemplate a

commercial cotton industry in near future…Cotton

CRC policy no commercial production could be

contemplated with anything less than Bollard II or

equivalent two gene types

 …Northern Australia…further information may be Ch 6, CBD &

required on potential weediness of GM Appendix 1

cotton…although…risk of weediness is…likely to be

low

 …see less urgency in a commercial approval for Noted

Northern Australia

 …provided on specific comment on certain clauses in Noted

the RARMP where there appear to be technical errors

or misunderstandings or where the basis for a clause is

unclear

38 A  supported OGTR‘s recommendations provided that Noted

there are no substantive additional risks to public

health and safety, or to the environment…the release of

Bollard II cotton without specific management

conditions be permitted below latitude 20 degrees south

39 C  …signed by 22 people from the Mildura region Noted

(Victoria) against the Commercial release

 …reasons include a release would violate the Ch 5

precautionary principle, violate the principle of Ch 6 & CBD

inter-generational equity, scientific uncertainty, lack of Ch 7

independent reliable public research data to verify

claims by Monsanto, enhancement of Bt resistance in

the insect population and possible spread of resistance

to closely related species, become a troublesome weed,

human health relating to bacterial antibiotic resistance

& lack of research into redesigning agricultural

methods so that genetically modified crops are not

needed

40 C  …signed by 23 people from the Katherine region Noted

against the Commercial Release…same as the

Katherine Town Council‘s letter

 …GMO trials are far from complete and suitability of Noted

the GM cotton is inconclusive to viability and

environmental sustainability

 …the weediness potential has not been established Ch 6, CBD & Ch

inconclusively 9



 …no evidence that the introduction will have no impact Ch 6

on the resistance to Roundup

 …no evidence that the gene traits of species will not Ch 7

spread to other non-target insects or plant species





APPENDIX 3

 …only research being trialed is based on agronomic OSA

aspects, not on the human health and environmental

sustainability aspects

 …research on the effect of this industry on water OSA

quality and tropical riverine environments has not

taken place

 …potential hazards of the GMO in the tropical north Ch 9

have not been fully addressed

41 C  …signed by 80 people from the Katherine region Noted

against the Commercial Release

 …aerial spraying of chemicals poses a risk to OSA

neighbouring flora, non-GM species and commercial

interests such as the cattle industry

 …no research in the public domain to show that GM Ch 6 & CBD

cotton poses no risk through weediness in northern

Australia

 …trials are focussing on agronomic aspects, not Ch 5

researching the potential impact to human health and Ch 6 & CBD

the environmental issues…specifically for this region Ch 7, Ch 8

 …Public Health Association of Australia has raised Ch 5

serious concerns about the effects of GMO‘s in humans

and questions research on the lacking of longitudinal

studies and long term survey of patient dietary habits

42 C [NB: this is an independent submission, despite its Noted

similarity to that of similar submissions including, for

example, submission no. 41]



 signed by 81 people from the Katherine region against

the Commercial Release

 …aerial spraying of chemicals poses a risk to OSA

neighbouring flora, non-GM species and commercial

interests such as the cattle industry

 …no research in the public domain to show that GM Ch 6 & CBD

cotton poses no risk through weediness in northern

Australia

 …trials are focussing on agronomic aspects, not Ch 5

researching the potential impact to human health and Ch 6 & CBD

the environmental issues…specifically for this region Ch 7, Ch 8

 …Public Health Association of Australia has raised Ch 5

serious concerns about the effects of GMO‘s in humans

and questions research on the lacking of longitudinal

studies and long term survey of patient dietary habits

43 A  …industry will be mindful of the social, economic and Noted

environmental management demands of the

marketplace and will have to work to have appropriate

responses / partnerships implemented to respond to

such demands…as long as it complies with legislation

and is fully researched





APPENDIX 3

 …supports the need to actively seek both internal and Noted

external stakeholder advice on how to use

biotechnology effectively

 …supports the need to consult with both internal and Noted

external stakeholders about research and ensure that

the information is made available to the public in a

balanced way regarding food, seed and fibre products

 …supports the need to collaborate with members of the Noted

wider agricultural community, local communities, end

users and environmental group stakeholders, develop a

set of measures to assess the effectiveness of the

application throughout the industry…preventing cross

contamination of other types of cotton, weed and insect

resistance, yield losses, increase in overall pesticide

usage, loss of crop or product quality etc.

 …supports the idea of promoting the adoption of a Noted

wider IPM system throughout the industry and the use

of this GMO…if it can be demonstrated not to have

negative effects

 …supports the work of our research bodies ensuring Noted

that the environmental and resistance issues are

carefully addressed and are confident of the result

presented

 …recognises the industry‘s commitment to proactive Noted

management of resistance and the importance it places

on compliance with resistance management strategies

required by the OGTR and NRA

 …supports the use of biotechnology where the benefits Noted

to growers, environment, human health and the end

user are significant

 …believes that members of the cotton industry should Noted

have the right to choose from a range of technologies

and production management systems that will

contribute to enhanced sustainability and lead to the

minimisation of environmental impact

44 C  …concerns regarding food…and the lack of Ch 5

information in the RARMP that actually deals with

public health and safety

 …claims Bt can cause allergy and there is no testing Ch 5

offered to show this is not the case with this

crop…other health matters such as carcinogenicity are

never addressed

 …concerns of losing some of Northern Australia‘s Ch 5

insect species (ie Butterflies) due to glyphosate‘s

affinity for these insects









APPENDIX 3

 …concerns regarding the link between Acrylamide and Ch 5

Glyphosate (a separate paper was attached)…and the

lack of investigation into a possible public health and

safety link by OGTR, NRA & FSANZ

 …raised the issue of weediness and gene transfer…and Ch 6 & CBD

should these genes transfer to other plants, the Ch 7

consequences or demise of beneficial or non-pest

insects

 …refers to a British Royal Society report (Sept 1998) Ch 5

on the call for ending the use of antibiotic resistance

marker genes in engineered food products…and that it

is a completely unacceptable risk, however slight, to

human health

 …herbicide tolerance gene transfers…the muddying of Ch 5

the gene pool cannot be good for either biodiversity or Ch 6 & CBD

the environment Ch 7

 …horizontal gene transfer has not been given enough Ch 7

consideration and is too lightly dismissed

 …due to the fact that genetically altered DNA has been Ch 5, Ch 7

found to able to transfer to the gut of other species,

council is of the opinion material from this trial should

not be used for either human or animal food

 …the call for all intentional commercial releases be Ch 9

monitored at least twice annually

45 I  …express our support and approval for the release of Noted

Bollard II for this planting season

 …growing INGARD cotton for five years… enormous Noted

reduction in use of pesticides…80%…trial data

suggests Bollard II…even more efficacious

 …has benefits in area of workplace health and safety Noted

and the environment

 …increased profitability and enhance sustainability Noted

46 C [NB: this is an independent submission, despite its Noted

similarity to that of similar submissions including, for

example, submission no. 41]



 …signed by 82 people from the Katherine region

against the Commercial Release

 …aerial spraying of chemicals poses a risk to OSA

neighbouring flora, non-GM species and commercial

interests such as the cattle industry

 …no research in the public domain to show that GM Ch 6 & CBD

cotton poses no risk through weediness in northern

Australia

 …trials are focussing on agronomic aspects, not Ch 5

researching the potential impact to human health and Ch 6 & CBD

the environmental issues…specifically for this region Ch 7, Ch 8







APPENDIX 3

 …Public Health Association of Australia has raised Ch 5

serious concerns about the effects of GMO‘s in humans

and questions research on the lacking of longitudinal

studies and long term survey of patient dietary habits

47 C  …supports the submission made by …National Council Noted

of Women [submission number 44]

48 I  …concern that there is still potential for adverse Ch 5

impact on humans due to potential

toxicity/allergenicity…even if potential is very low,

there is still a likelihood of occurrence

 …have studies been carried out over generations and Noted

across broad sections of all communities or has

company research been only recent….less than one

generation

 …even if potential for weediness is low in Roundup Ch 2, Ch 6 &

species [cotton]…a risk still exists, making it CBD

potentially unsafe to the environment

 ...if risk of gene transfer is high…potential for harm Ch 7

to environment since this is a trial and long term

effects are unknown

 …if there is even a low risk of Bollard II…becoming a Ch 6, CBD, Ch 9

weed in northern Australia…should not be released in & Appendix 1

this area…commercially or trialed due to

environment risk…would be a huge community

problem of containing and ‗weediness‘ and gene

transfer especially where licence conditions to limit

spread are not carried out stringently.

 …what will happen if herbicide tolerance is Ch 2, Ch 6 &

transferred to already noxious weeds or plants which CBD & Ch 9

have potential to become weeds?

 ……how do we foresee that insecticide resistance Ch 8

management strategies will not be breached by

unscrupulous persons or companies

 …larger danger to environment due to potential for Ch 2, Ch 6 &

herbicide resistant weeds developing where crop CBD & Ch 9

herbicide combination is used inappropriately.

 …whole point of ‗Roundup ready cotton‘…spray more Ch 5

heavily with herbicide…poses potential health risks to Ch 6 & CBD

humans and to environment if overspraying occurs Ch 7, Ch 8

 [Leading to] ...increased levels of herbicide in the plant Ch 2 & Ch 5

will be transferred to the food chain with increased

human consumption of herbicide

 [Leading to] ...more herbicide washed into the Ch 2 & Ch 5

environment – do we know the long term effects of this

chemical over 50 years or more

 …glyphosate…is not used for control of cotton plants OSA

in agriculture or the natural environment…why do we

need Roundup ready cotton at all?





APPENDIX 3

 …[jeopardise] huge potential billion dollar export OSA

market for GE free produce









APPENDIX 3


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