diagnostic_detectives_urinalysis_lesson_plan by thMXdAqM


									Urinalysis Lesson Plan - “It’s Not Just Water “

                                     Urinalysis Lesson Plan
                                     “It’s Not Just Water “

Grade Levels(s):       9,10,11,12, Occupational/Technical Health

Subject:               Science / Biology / Occupational Health

National Science Content Standards:
 Life Science, Cells – compare and contrast ways in which selected cells are specialized to
   carry out particular life functions (e.g., classifications by cell types such as red blood cells,
   white blood cells, muscle cells, etc.; specialized cell functions such as transport,
   photosynthesis, etc.)
 Life Science, Organization of Living Things – explain how living things maintain a stable
   internal environment (e.g., related systems/cells/chemicals – excretory system, circulatory
   system, endocrine system; factors/mechanisms under control – disease/infection,
 Life Science, Organization of Living Things – describe technology used in the prevention,
   diagnosis, and treatment of diseases (e.g., biochemistry; health monitoring activities)
 Physical Science, Changes in Matter – explain chemical changes in terms of breaking of
   bonds and the rearrangement of atoms to form new substances.
 Constructing New Scientific Knowledge, Design and Conduct Investigations using
   Appropriate Methodology and Technology – recognize and explain limitations of measuring
   devices; gather and synthesize information from books and other sources of information

1. To review the structure and function of the kidney.
2. To demonstrate to students the importance of Urinalysis as it relates to patient care.
3. To demonstrate to students how chemical and microscopic cell analyses relate to
4. To introduce students to various urine abnormalities via case study presentations.
5. To introduce students to the roles of various clinical laboratory professionals.

Student Objectives:
Upon completion of the lecture presentation and laboratory analysis, students will be able to:
1. Describe the major functions of the kidney
2. List the major structures of the kidney involved in urine formation.
3. Describe the importance of performing Urinalysis.
4. Observe the various urine elements detected via dipstick chemical analysis.
5. Observe the basic cell types found in the microscopic analysis of urine sediment.
6. Name the clinical laboratory professionals who perform urinalysis testing and explain the
    education requirements of each.

Materials Needed:
1. Test tubes (plastic) and test tube rack
1. Mock urine samples (see Recipes section below)
2. Urine test strips (Bayer MULTISTIX® 10SG Reagent Strips for Urinalysis and product
    insert - available at local pharmacies or medical supply stores)
3. Handouts (provided in the Urinalysis Lesson section of the Teacher’s Toolkit):
     Urinalysis Laboratory Worksheets (includes answers and grading rubric)
     MULTISTIX 10SG Urinalysis Reagent Strip Information Sheet
5. Computer for “It’s Not Just Water” PowerPoint presentation and microscopic images.

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Urinalysis Lesson Plan - “It’s Not Just Water “

Pre-requisites / Background Information:
1. Information presented in the lecture presentation titled “It’s Not Just Water”.
2. Teacher should review information in MULTISTIX product insert before Day 2 lab

Procedures – Day 1 – Lecture :
1. Lecture: Complete the pre-requisite background information presentation “It’s Not Just
    Pee”. (Approximately 45 minutes.)

Procedures – Day 2 – Chemical and Microscopic Analysis of Urine:
1. Divide students into groups of 2-4.
2. Distribute urine samples #1-4 and MULTISTIX® dipsticks to each group.
3. Distribute:
    a) MULTISTIX® 10SG product insert containing dipstick procedure and
    b) MULTISTIX® 10SG Test Strip Information handout.
4. Have students perform visual dipstick analysis on each urine sample and record results
    on their worksheet.
    NOTE: Stress the importance of proper read times for the different test areas.
5. Have students view microscopic images from each sample on the computer and record
    their observations on their worksheet. (NOTE: Microscopic images are provided at the
    end of the “It’s Not Just Water” presentation.)

Grading Rubric:
1. Correct performance of urine dipstick analysis                              30 pts
2. Neat completion of dipstick analysis worksheet for all five samples         30 pts
3. Correct results for each urine sample (N=4)                                 20 pts
4. Correct identification of microscopic cells for each sample.                20 pts
5. EXTRA CREDIT: State possible clinical conditions for each sample.           (10 pts)
6. Grade = Total Points / 100

Mock Urine Recipes (can be made up the day before and refrigerated):
1. Sample #1: 14 year old girl with thirst and fatigue - Mountain Dew (flat)
2. Sample #2: 85 year old man with kidney stone – 100 mL of chicken broth ( or one cup
    cooled chicken bouillon made with a bouillon cube or powder) with just a few drops of
    blood from raw ground beef or turkey added
3. Sample #3: 18 year old female with urine frequency – 5 mL (1 teaspoon) of ½ percent
    milk added to 45 mL water or bouillon.
4. Sample # 4: 16 year old male undergoing sports physical – chicken bouillon

1. Urinalysis and Body Fluids – A ColorText and Atlas, Ringsrud, K and Linne, J, Mosby-
    Year Book, Inc., St. Louis, 1996
2. BAYER Diagnostics MULTISTIX® SG10 product insert AN30516C, Bayer Corporation,
     Revised April, 1999
3. Encyclopedia of Urinalysis, CD-ROM – version 2.2, BAYER Diagnostics, 2002
4. MTS Lab Training Library CD-ROM, University of Washington, Department of
    Laboratory Medicine, 2003.
5. Medline Medical Encyclopedia - Urinalysis

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Urinalysis Lesson Plan - “It’s Not Just Water “

Supplemental Activities:
1. Using the product insert, have students write a description of the chemical reaction
    occurring on each reagent test area of the urinalysis strip.
2. Web Quest: Listed below are various web sites with web-based case studies, tutorials,
    activities or additional educational information about Urinalysis.
       Urinalysis Cases and Images – continually developed cases from the University of
            Delaware Medical Technology Department
       Web Path / Med Web – Urine Tutorial
       Lab on a Stick - an article about the development of the urinalysis test strip by
            researcher Helen Free, including review activities in the Teacher's Guide. From the
            American Chemical Society October, 2004 ChemMatters newsletter for high school
       Urine: Your Own Chemistry - an interesting article about the function,
            composition and analysis of urine. From the American Chemical Society October,
            2002 ChemMatters newsletter for high school students.
       TutorGig – site of comprehensive urinalysis tutorial links and urinalysis product
            ordering information.
       University of Hawaii – MedTechTutor – numerous tutorials on Urinalysis as well as
            General Lab, Hematology and Hemostasis.
       Lehigh Career and Technical Institute – collection of lesson plans; see pages 32-34
            of pdf document for Urinalysis lesson plan
       The Nephron Information Center – How the Kidney Works – excellent interactive
            simulation of how the kidney filters blood.
       The Kidney Quiz – simple quiz provided by the National Kidney Foundation

3. Toxicology Case Studies located in the Clinical Pathology-Chemistry lessons in the
   Teacher Tools portion of this CD/web site. The cases discuss how urine specimens are
   analyzed in the Toxicology laboratory for the detection of therapeutic and illegal drugs.

4. Kidney Word Jumble and Urinalysis Crossword (located in Handout Masters section)

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