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Walker
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Emerging Anticoagulants

for VTE Prevention

and Treatment:

Is change upon us?

Amanda C. Walker, PharmD

Clinical Pharmacist

University of Utah

University Thrombosis Service

Objectives



 Describe the pharmacology and mechanism of

action for new and emerging anti-thrombotic

agents

 Discuss and review current randomized

controlled trials for new and emerging anti-

thrombotic agents for VTE prevention and

treatment

 Evaluate the adverse drug reactions and side

effects associated with these new agents

Current Limitations

Limitations Consequences

► Parenteral ► Inconvenient for

UFH ► Unpredictable due to long term use

unspecific binding ► Monitoring of

► Risk of HIT aPTT required

► Parenteral ► Inconvenient and

LMWH ► Risk of HIT expensive for long term

use

► Monitoring of platelets

► Unpredictable ► Regular monitoring and

VKAs ► Slow onset of action dose adjustments

► Narrow therapeutic window ► Risk of adverse events

► Food and drug interactions

(bleeding)

► Parenteral ► Inconvenient and

Fondaparinux

expensive for long term

use

Lassen MR. Vasc Health Risk Manag. 2008;4(6):1373-86.

New and Emerging

Anticoagulants

 Anti – Xa : direct  Anti – IIa

 Rivaroxaban (oral)  Dabigatran (oral)

 Apixaban (oral)  Odiparcil (oral)

 Betrixiban (oral)

 Flovagatran (parenteral)

 Edoxaban (oral)

 Pegmusirudin (parenteral)

 Otamixaban (parenteral)

 Peg Hirudin

 LY – 517717 (oral)

 DU – 176B (oral)  Desiruidin

 DX – 9065a (parenteral)

 PRT054021 (oral)



 Anti – Xa : indirect

 Idraparinux biotinylated

(parenteral)

Site of Action for New

Anti-thrombotic Agents

Intrinsic XII Extrinsic



XI Tissue Factor



IX

VIII VII

Indirect Xa Direct Xa Inhibitors

Inhibitors AT X

“-xaban”

“-parinux”

V

Direct Thrombin

Inhibitors

warfarin II

“-gatran”

Fibrinogen



Fibrin Clot

Factor Xa vs. Factor IIa



 Factor Xa  Factor IIa

 One Xa forms many  Supports feedback

IIa amplification through

 Limited role in Factor V, Factor VIII,

diversity of action and Factor IX

outside of coagulation  Has many cellular

cascade effects

 Clinical effectiveness  inflammation

 Fondaparinux  Clinical effectiveness

 Argatroban

 Hirudins

Direct Factor Xa Inhibitors

Intrinsic XII

XI Extrinsic

TF

IX

VIII VII

Direct Xa Inhibitors

X “-xaban”



V

II

Fibrinogen





Fibrin Clot

Apixaban

 Oral tablet

 Bioavailability: 50%

 Eliminated via

 Peak Plasma Levels multiple pathways

= 3 hrs  No laboratory

 Half-life ~ 12 hours monitoring required

 Metabolized in liver  Manufactured by

via CYP3A4 and Bristol-Myers

CYP independent Squibb/Pfizer

mechanisms  Plan to submit for

U.S. approval in

2009-2010

Apixaban Efficacy

Outcomes in TKR (Phase II)

Incidence of VTE and all-cause death (%)

40



35

30 Duration =

10 -14 days

25

20



15

10



5

0

5 2.5 10 5 20 10 Enox Warf

QDay BID QDay BID QDay BID 30mg BID

(n=152) (n=153)

Apixaban (mg) (n = 933)

Lassen MR, et al. J Thromb Haemost. 2007;5:2368 – 2375.

Apixaban Safety

Outcomes in TKR (Phase II)

Incidence of bleeding events (%)

18

16

14

12

10

8

6

4

2

0

5 2.5 10 5 20 10 Enox Warf

QDay BID QDay BID QDay BID 30mg BID

(n=152) (n=153)

Apixaban (mg) (n = 933)

Apixaban Phase III Trials

Medically

Knee Knee Hip

Ill



# Patients 3058 3058 4022 6524

ADVANCE-1 ADVANCE-2 ADVANCE-3 ADOPT





Est. Completion March

Oct 2008 May 2009 Feb 2009

Date 2009



Apixaban Apixaban

Apixaban 2.5mg BID x

Apixaban 2.5mg BID

2.5mg BID 30 days

2.5mg BID vs vs

Study Arms vs

vs Enox Enox

30mg BID Enox Enox 40mg

40mg 40mg QDay QDay x 6-14

QDay days

ADVANCE – 1: Results of Efficacy

vs. Enoxaparin 30 mg BID

RR: 1.02 (95% CI: 0.78 to 1.32) P=0.06 for non-inferiority

Absolute Difference: 0.1% (95% CI: -2.22 to 2.44) P80%)  No dosage adjustment

 Onset of action 2-4 for gender, age, extreme

hours body weight

 Half-life 9-12 hours  Approved by Europe and

 No observed effects on Canadian agencies, and

agonist-induced platelet under FDA review

aggregation currently

Rivaroxaban in VTE Prevention:

RECORD 3 - TKA

2531 patients

% %6

20 No Difference

18 5

16

RRR 4

14

12 RRR 62%

3

10 49%

8 2

6

4 1

2

0 0

Rivarox 10 Enox 40 Qday Rivarox 10 Enox 40 Qday

Qday x 14 d x 14 days Qday



Composite Major VTE Major Bleed Any Bleed

Rivaroxaban in VTE

Prevention:

3034 patients

RECORD 4 - TKA

Rivarox: RRR % 3.5

% 10 31%; ARR 3.2%

3 Not Significant

8 2.5



6 2



4 1.5



1

2

0.5

0

Rivarox 10 mg Enox 30 mg BID 0

Qday Rivarox 10 mg Enox 30 mg BID

Qday

Composite

Major Bleed Any Bleed

Symptomatic VTE and all-cause mortality

Turpie, et al. Lancet 2009;373:1673 – 80.

Rivaroxaban Ongoing

Phase III Clinical Trials

DVT PE DVT/PE

Einstein-DVT Einstein-PE Einstein-Extension

Rivarox 15mg BID x 3 Rivarox 15mg BID x 3 Rivarox 20mg Qday

wks then 20mg Qday wks then 20mg Qday vs

vs vs Placebo

Enox/VKA Enox/VKA







AF Medically Ill

Rivarox 20 mg Qday

vs Rivarox 10mg Qday x 35 days

Warfarin vs

Enox 40mg Qday x 10 days

Indirect Factor Xa

Inhibitors

Intrinsic XII

XI Extrinsic

TF

IX

VIII VII

Indirect Xa

Inhibitors AT

X

“-parinux” V

II

Fibrinogen





Fibrin Clot

Idraparinux

 Once weekly SC injection

 100% SC bioavailability

 Half-life ~ 96-130 hours

 Renal elimination

 No monitoring required

 Manufactured by

Sanofi-Aventis

 Plan to file for U.S.

approval in 2009

Van Gogh Trials

Idraparinux 2.5 mg SC qweek vs standard therapy (heparin/LMWH + VKA)







DVT Study PE Study VTE Extended Study

Idraparinux vs placebo





2904 patients 2215 patients 1215 patients

↔ 3- and 6-month ↑ 3- and 6-month ↓ recurrent events

recurrence recurrence ↑ bleeding

↓ bleeding at 3 mo ↓ bleeding at 3 and ↔ mortality

↔ bleeding at 6 mo 6 mo

↔ mortality ↑ mortality

Amadeus Trial

4576 patients







 Non-valvular atrial

fibrillation

 Idraparinux 2.5 mg

SC qweek vs VKA

 Trial stopped early

due to excess

clinically relevant

bleeding with

idraparinux

Idraparinux Biotinylated



MeO

N

OSO 3 a

N

OSO 3 a S

NaO 3 O

NaO 3 O

S O

O

NaOOC

O O O

O Idraparinux sodium

O OM e

e

MO O O NaOOC S

NaO 3 O

MeO MeO M eO OM e OM e NaO SO

3

N

OSO 3 a









M eO

O SO 3Na

N

O SO 3 a S

NaO 3 O

N aO 3 O

S O

O O O O

NaO OC O

MO

e O O N aO O C

O

N aO 3 O

S

OM e Idraparinux

O M e N aO SO

Biotinylated

M eO HN M eO OM e 3

N

O SO 3 a



O S





Avidin

H

N NH



HN

O O



Biotin arm with spacer

•No pharmacological effect

•IV injection

•Short half-life (10-16 min)

Phase III Clinical Trials with

Idraparinux Biotinylated

Idraparinux

biotinylated

3 mg weekly

vs

warfarin

in 6-month PE

treatment

(3200 patients)

Idraparinux biotinylated

3 mg weekly

vs

BOREALIS-AF

idraparinux

2.5 mg weekly Idraparinux biotinylated 3 mg weekly

in 6-month DVT tx vs

(700 patients) warfarin

in AF (9600 patients)

Direct Thrombin Inhibitors

Intrinsic XII

XI Extrinsic

TF

IX

VIII VII

X

V

Direct Thrombin

II Inhibitors

“-gatran”



Fibrinogen





Fibrin Clot

COMPANY NEWS; COMPANY NEWS;

F.D.A. PANEL F.D.A. REJECTS

VOTES AGAINST ASTRAZENECA'S

BACKING DRUG BY ANTI-CLOTTING

ASTRAZENECA DRUG

Published: September 11, 2004 Published: October 9, 2004



AstraZeneca failed to win AstraZeneca said yesterday

backing from a federal that federal regulators did not

government panel for its approve its anti-clotting drug,

Exanta blood thinner, a Exanta.

possible first alternative to the

drug Coumadin in more than

50 years.

Dabigatran

 Oral capsule  No dietary/food

 Rapid onset of action interactions

 Half-life 12-17 hours  Brand name Rendix™

or Pradaxa®,

 Renal elimination Boehringer-Ingelheim

 No routine monitoring  Approved in Europe

required March 2008; plans

 P-gp substrate—use are to obtain U.S.

with caution when FDA approval by

administered 2010

concomitantly with P-

gp inhibitors

Dabigatran in TKR:

RE-MODEL (Phase II)

% Total VTE & Death % Adverse Events

41 12

n=1541 patients

40

treated 6-10

days, followed

10

for 3 months

8

39 post-surgery

6

38

4

37 2

36 0

150 mg 220 mg Enox 40

35

Qday Qday Qday

34

Dabigatran

150 mg 220 mg Enox 40

Qday Qday Qday

Major Bleeding Minor Bleeding

Dabigatran

LFT > 3xULN

Dabigatran in THR:

RE-NOVATE (Phase II)

% Total VTE & Mortality % Adverse Events

n=2651 patients

10 treated 28-35 7

days, followed 6

9 for 3 months

post-surgery 5

8 4

7 3

6 2

5 1

4 0

3 150 mg 220 mg Enox 40

2 Qday Qday Qday

1

Dabigatran

0

150 mg 220 mg Enox 40

Qday Qday Qday

Major Bleeding Minor Bleeding

LFT >3xULN

Dabigatran



Eriksson BI et al. Lancet 2007;370:949-56.

Dabigatran: RE-VOLUTION

Trial Program (Phase III)

AF and Secondary

Acute VTE Stroke VTE Secondary VTE

Treatment Prevention Prevention Prevention

RE-COVER RE-MEDY

RE-LY RE-SONATE

# Patients 2554 18000 1800 2004

Est

Completion Dec 2009 June 2009 October 2009 January 2011

Date



Dabigatran

Dabigatran 100 mg BID Dabigatran Dabigatran

150 mg BID and 150 mg 150 mg BID 150 mg BID

Study Arms BID

vs vs vs

warfarin vs placebo warfarin

warfarin

Summary

Time to Market for New Anti-Thrombotic Agents



Apixaban

Dabigatran

Otomaxiban

Idraparinux

Rivaroxaban

biotinylated



2010 2011 2012 2013

Property Rivaroxaban Apixaban Idraparinux Dabigatran

Target Factor Xa Factor Xa Factor Xa Thrombin

(indirect)

ROA Oral Oral Subcutaneous Oral



Prodrug No No Yes Yes



Bioavailability > 80% > 50% 100% 6%



Time to peak 3 3 ___ 2



Half-life 9 hrs 9 – 14 hrs 80 hrs 14 – 17 hrs



Frequency of Qday BID Q Week Qday or BID

Administration

Drug Potent CYP3A4 & Potent CYP3A4 & ___ P-glycoprotein

P-glycoprotein P-glycoprotein inhibitors

Interactions inhibitors inhibitors



Renal 66% 25% Yes 80%

excretion

Safe in No No Unknown No

pregnancy

Antidote No No No No

Adapted from: Gross, PL. Arterioscler Thromb Vasc Biol. 2008; 28:380-386.

Potential Limitations of

New Anticoagulants

 Antidotes

 None of the newer agents has a specific antidote

 Monitoring

 Adverse Drug Events

 Compliance

 Cost

 Clinical Trials vs. Actual Clinical Practice

 Patient populations not even studied (i.e.

Cancer)

Conclusion



 Several oral and parenteral Anti Xa and Anti IIa

drugs are under development at this time

 Rivaroxaban and Dabigatran are approved in

the European Union and Canada for the

prophylaxis of DVT and awaiting FDA

review/approval

 Safety issues are of prime importance in the

development of these drugs and will be

strongly scrutinized upon review

What about RE-LY?

Dabigatran versus Warfarin in Patients with

Atrial Fibrillation

 Non-inferiority trial

 Over 18,000 patients

 Followup = 2 years



Dabigatran 110 mg and 150mg

vs.

Adjusted dose warfarin


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