Identified
Pred No. Coverage phoshorylation Known Phosphorylation sites (from SwissProt, PhosphoSite
IPI Acc. No. Protein name Gel MW MW Pred pI peptides (%) Score Matched peptides (at least 2 shown) sites & PHOSIDA) Function
IPI00431026 Abl interactor 1 isoform 2/Spectrin 65000 48679 6.87 3 8 53/36 ALIESYQNLTR; pY22; pT173; pT177; pS182; pS186; pT190; pY197; pT199; May act in negative regulation of cell growth and transformation by
SH3 domain-binding protein 1 KPIDYTVLDDVGHGVK pY212; pT214; pS215; pS221; pS224; pS230; pY332; pT335; interacting with nonreceptor tyrosine kinases ABL1 and 2; may play role in
pS360; pY454; pY456 regulation of EGF-induced ERK activation; involved in cytoskeletal
reorganization and EGFR signalling; together with EPS8 participates in
transduction of signals from Ras to Rac; interacts with many proteins
including ABL1, MENA, EPS8, SOS1, SOS2, GRB2, NCK1 and NCKAP1
IPI00025416 Actin gamma-2/ACTG2 40000 41877 5.19 3 9 94/36 AGFAGDDAPR; HQGVMVGMGQK pS34; pY54; pY70; pS200; pS324; pT325; pS240 Major cytoskeletal protein
IPI00021439 Actin, cytoplasmic 1/beta-actin 40000 41737 5.15 15 35 277/37 QEYDESGPSIVHR; DSYVGDEAQSK pS33; pY53; pY91; pY166; pY169; pY188; pY198; pT203; pY218; Major cytoskeletal protein
pS232; pS233; pS239; pY240; pY294; pT318; pS323; pT324;
pY362
IPI00023461 Afadin, isoform 4/MLLT4 180000 206806 6.41 11 7 81/37 LAAGDQLLSVDGR; IVQATTLLTMDK pY94; pY202; pS215; pY262; pY374; pS545; pS548; pS549; Adhesion molecule; plays a role with the E-cadherin-catenin system in
pY970; pS1066; pS1090; pS1107; pS1155; pS1156; pS1165; organization of homotypic, interneuronal and heterotypic cell-cell adherens
pS1184; pY1213; pT1215; pS1258; pY1268; pY1480; pY1675; junctions (AJs); multiple interactors including F-actin, E- cadherin, ZO-1 and
pS1679; pS1681; pS1704 occludin
IPI00013508 Alpha-actinin 1 100000 103059 5.07 12 16 180/38 QKDYETATLSEIK; ALDFIASK pY12; pT50; pS140; pY193; pY215; pY246; pS404; pT737 F-actin cross-linking protein which is thought to anchor actin to a variety of
intracellular structures
IPI00013808 Alpha-actinin 4 100000 104855 5.12 10 14 211/38 ALDFIASK; MAPYQGPDAVPGALDYK pS159; pY234; pY265; pS423; pS447; pS461; pS621; pT667; F-actin cross-linking protein which is thought to anchor actin to a variety of
pT682; pT756 intracellular structures
IPI00414320 Annexin A11 65000 54697 7.53 4 14 49/38 GVGTDEACLIEILASR; pY279; pY365; pY482 Ca2+-dependent phospholipid binding
GFGTDEQAIIDCLGSR
IPI00384861 ARF GTPase-activating protein 65000 42294 6.07 6 12 105/38 RPALEPVR; SLSSPTDNLELSLR pS359; S361; pS362; pT364; pS373; pY383; pS385; pS388; ARF GTPase-activating protein; may serve as a scaffold for signalling
GIT1 pT392; pS410; pS413; pS414; pS417; pS508; pS514; pS536; modules controlling vesicle trafficking, adhesion, migration and cytoskeletal
pT537; pY545; pY554; pS570; pS571; pS592; pS596; pY598; organization; interacts with G protein-coupled receptor kinases, paxillin,
pT601; pS700 PTK2, FAK and PAK1
IPI00215637 ATP-dependent RNA helicase 65000 73272 7.2 15 21 287/37 VGSTSENITQK; YLVLDEADR pY52; pS60; pS61; pY68; pS73; pS75; pS77; pS81; pS91; pY103; Belongs to DEAD box RNA helicase family
DDX3X pS124; pS130; pT203; pY242; pY282; pY300; pT322; pY342;
pY461; pS593; pS611; pS613
IPI00179053 Breast cancer antiestrogen 100000 92567 8.02 9 9 152/38 DGDFLVR; LSLTMGGVQAR pS32; pY42; pS78; pS83; pT86; pT130; pY117; pS182; pY212; Adapter protein coupling activated growth factor receptors to a proliferation
resistance 3/BCAR3 pY266; pS290; pT292; pS358; pS363; pS375; pY429 signalling pathway in breast cancer cells; confers anti-estrogen resistance;
may be regulated by adhesion to ECM
IPI00216677 Cadherin-3/P-cadherin 130000 91428 4.37 4 6 52/37 QITICNQSPVR; GLEARPEVVLR - Ca2+-dependent cell-cell adhesion glycoprotein; overexpressed in breast
carcinomas
IPI00021536 Calmodulin-like protein 5 140000 15921 4.05 4 45 139/36 AGLEDLQVAFR; - Ca2+ binding protein; may be involved in terminal differentiation of
AFSAVDTDGNGTINAQELGAALK keratinocytes
IPI00215948 Catenin alpha-1, splice isoform 1 100000 100693 5.95 30 36 598/38 ALKPEVDKLNIMAAK; pS83; pS118; pY177; pY280; pS323; pS361; pT372; pY419; Cell-cell adhesion through cadherins
VIHVVTSEMDNYEPGVYTEK pT541; pY619; pT634; pS641; pT645; pS652; pT654; pS655;
pT658; pS668
IPI00473136 Catenin alpha-1, splice isoform 2 100000 102777 6.25 23 27 403/38 TAVHAGNINFK; HVNPVQALSEFK pS83; pS118; pY177; pY280; pS323; pS361; pT372; pY419; Cell-cell adhesion through cadherins
pT541; pY619; pT634; pS641; pT645; pS652; pT654; pS655;
pT658; pS668
IPI00385055 Catenin alpha-2 100000 100448 5.72 13 27 403/38 WDDSGNDIIVLAK; pT5; pS6; pS132; pT135; pS151; pS652; pT654; pS655 Cell-cell adhesion through cadherins
TSVQTEDDQLIAGQSAR
IPI00017292 Catenin beta-1 100000 85442 5.53 8 12 203/37 LSVELTSSLFR; VAAGVLCELAQDK pY30; pS33; pS37; pT41; pS45; pY86; pY142; pS191; pS196; Adherens junction protein; forms complex with PSEN1-cadherin-catenin
pS246; pY331; pY333; pT393; pT472; pS473; pY489; pT551; alpha to anchor actin cytoskeleton; involved in signal transduction and
pS552; pT556; pS605; pY654; pY670; pS675; pY716 transcriptional regulation through Wnt pathway
IPI00219741 Catenin delta-1/Cadherin- 100000 91221 6.93 39 36 686/37 GSLASLDSLRK; pY228 pS4; pT27; pS38; pS47; pT59; pY96; pS97; pT98; pY112; pT134; Regulates cell adhesion of C- and E-cadherins; inhibits transcriptional
associated Src substrate/p120 HYEDGYPGGSDNpYGSLSR; pT152; pS169; pS171; pY174; pT177; pY193; pT199; pY208; repressor ZBTB33 leading to activation of target genes of Wnt signalling;
catenin VGGSSVDLHR; SLDNNYSTPNER; pY213; pY217; pY221; pS225; pY228; pS230; pS232; pS244; implicated in cell transformation by SRC and in ligand-induced receptor
pY248; pS252; pY257; pS268; pS269; pY280; pS288; pY291; signalling through EGFR, PDGFR, CSF1R & ERBB2 receptors
pY296; pS300; pY302; pT308; pT310; pS312; pS320; pY321;
pY334; pY335; pS346; pS349; pS352; pS539; pS542; pS587;
pY600; pS651; pY656; pS675; pT720; pS731; pT774; pS777;
pS847; pS857; pS861; pS862; pS864; pY865; pS868; pT869;
pS879; pS899; pY904; pT906; pT916; pS920; pT932; pT933;
IPI00005264 Catenin delta-2/Plakophilin 2 100000 97400 9.73 6 8 149/38 IQEQVQQTLAR; YSQNIYIQNR pT80; pS81; pS82; pY86; pY88; pY119; pY130; pS132; pS135; Junctional plaque protein belonging to the beta-catenin family
pS151; pS154; pS155; pY161; pS164; pY166; pY168; pS169;
pS197; pY217; pY261; pS329; pY631; pY659
IPI00029601 Cortactin-Src substrate/CTTN 80000 61636 5.05 47 39 647/37 LPpSSPVpYEDAASFK; pS405; pY421; pT43; pY104; pS113; pS117; pY141; pS150; pY154; pY178; Regulates interactions between components of adherens-type junctions;
TQTPPVpSPAPQPTEER; pS418 pY215; pY265; pS282; pS303; pY334; pT399; pT401; pS405; regulates cytoskeleton and cell adhesion structures of epithelila and
GPVSGTEPEPVYSMEAADYR pT411; pS417; pS418; pY421; pS426; pS432; pY433; pS438; carcinoma cells; aberrant regulation contributes to tumor cell invasion and
pY446; pY453; pY470; pY486 metastasis
IPI00011998 CRK-associated 130000 93363 5.41 19 17 209/37 RPGPGTLpYDVPR; SAVGNAAHTSDR pY12; pY128; pS134; pS139; pY165; pY222; pY224; pY234; Docking protein which coordinaesr tyrosine-kinase-based signalling related
substrate/p130CAS/BCAR1 pY249; pY267; pT269; pY287; pS292; pY306; pY327; pY362; to cell adhesion; implicated in cell migration; overexpression confers
pY372; pY387; pY410; pS639; pY664; pY666 antiestrogen resistance in breast cancer cells; tyrosine-phosphorylated in
cells transformed by v-Crk or v-Src; interacts with FAK, PYK2 and PTP-1B
IPI00290039 CUB domain-containing protein 100000 92875 8.09 4 4 78/38 IDATVVR; LATEEPPPR pS9; pS142; pS164; pT170; pS184; pS210; pS575; pS579; pY707; Transmembrane protein involved in cell-adhesion and cell-matrix
1/CD318 pY734; pY743; pY762; pY806 association; plays a role in the regulation of anchorage versus migration or
proliferation versus differentiation via its phosphorylation; interacts with N-
cadherin, P-cadherin, syndecans-1 and -4, ST14, SRC and PKCgamma
IPI00554788 Cytokeratin 18 50000 47897 5.34 39 57 612/38 AQIFANTVDNAR; pS6; pT7; pS9; pY12; pS14; pS17; pS22; pY23; pS29; pS30; Intermediate filament protein; cytoskeletal component
GLQAQIASSGLTVEVDAPK pS33; pY35; pS41; pS50; pT51; pS52; pS59; pT64; pY128; pT301;
pS322; pS397; pS398; pS400; pT403
IPI00013933 Desmoplakin, isoform 1 140000 331780 6.78 8 2 40/37 DLKDEIVR; SVQNDSQAIAEVLNQLK pS22; pY28; pY41; pY56; pY95; pS165; pS166; pY172; pT173; Involved in organization of desmosomal cadherin-plakoglobin complexes
pS176; pS178; pS246; pY249; pY1139; pY1169; pT1220; pY1542; into discrete plasma membrane domains and in anchoring intermediate
pS1658; pS2024; pS2209; pY2275; pT2337; pY2339; pS2551; filaments to desmosomes; interacts with plakophilin 1 and junction
pT2555; pT2603; pS2606; pS2607; pS2608; pS2610; pT2612; plakoglobin
pS2815; pS2820; pS2821; pS2825; pS2827; pS2829; pS2833;
pS2849; pT2853; pS2856; pS2857; pY2858; pS2867; pS2868
IPI00217182 Desmoplakin, isoform 2 140000 260122 6.94 6 2 50/37 YGDGIQLTR; IEVLEEELR pS22; pY28; pY41; pY56; pY95; pS165; pS166; pY172; pT173; Involved in organization of desmosomal cadherin-plakoglobin complexes
pS176; pS178; pS246; pY249; pY1139; pY1169; pT1220; pY1542; into discrete plasma membrane domains and in anchoring intermediate
pS1609; pS1658; pT1737; pY1739; pS2006; pS2007; pS2008; filaments to desmosomes; interacts with plakophilin 1 and junction
pS2010; pT2012; pS2024; pS2209; pY2275; pS2551; pT2555; plakoglobin
pT2603; pS2606; pS2607; pS2608; pS2610; pS2815; pS2820;
pS2821; pS2825; pS2827; pS2829; pS2833; pS2849; pT2853;
pS2856; pS2857; pY2858; pS2867; pS2868
IPI00025861 E-cadherin 130000 99694 4.38 10 10 252/37 TIFFCER; VTEPLDRER pT211; pT330; pT394; pT395; pT576; pY753; pY754; pS838; Calcium-dependent adhesion protein involved in homotypic cell-cell
pS844; pS847; pS850; pS851; pS853 adhesion; potent suppressor of invasion; ligand for integrin alpha- E/beta-7;
role in cell-typesorting during morphogenesis; regulation of tight and gap
junctions
IPI00001466 Echinoderm microtubule- 130000 108904 6.27 6 7 40/37 DLLLTCAQDR; EIEVPDQYGTIR pS87; pS132; pS134; pS138; pS144; pS146; pY226; pY265; Modifies the assembly and dynamics of microtubules
associated protein-like 4 pY499; pS502; pT889; pS895; pT897; pT899; pS903; pT909;
pS919; pT921; pS934; pS938; pS942; pS946; pS978; pS981
IPI00025447* Elongation factor 1-alpha/EEF1A1 50000 50069 9.59 5 10 85/35 STTTGHLIYK; EVSTYIKK Promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of
protein ribosomes during protein biosynthesis
IPI00472724* Eukaryotic translation elongation 50000 50153 9.15 5 10 85/35 pY29; pY85; pY86; pY141; pY162; pS163; pY233; pY254; pT432
factor 1 alpha-like 3
IPI00639885* Elongation factor 1-alpha 1, 50000 50102 9.24 5 10 85/35
similar protein
IPI00021267 Ephrin type-A receptor 2 140000 108255 6.05 4 4 44/37 VSDFGLSR; VIGAGEFGEVYK pS426; pS433; pT437; pS570; pY575; pS577; pS579; pT587; Mediates cell contact-dependent repulsion; binds to ephrins A1, A3, A4 and
pY588; pT593; pY594; pY628; pT647; pY694; pT771; pY772; A5
pT773; pT774; pS775; pS790; pY791; pS892; pS897; pT898;
pS899; pS901; pY921; pT922; pY930; pT940; pY960; pS961
IPI00018274 Epidermal growth factor receptor 180000 134279 6.67 46 31 819/38 GSTAENAEpYLR; pY1197, pT693 pY270; pY316; pT678; pT693; pS695; pT725; pY727; Receptor tyrosine kinase; control of cell proliferation, differentiation and
ELVEPLpTPSGEAPNQALLR; pS752;pY764; pS768; pY869; pY944; pY978; pS991; pT993; survival; receptor for various EGF-related peptides
MHLPSPTDSNFYR; pS995; pY998; pY1016; pS1025; pS1026; pS1028; pT1029;
LLGAEEKEYHAEGGK pS1030; pS1036; pS1037; pS1039; pT1041; pS1042; pS1045;
pT1046; pS1064; pY1069; pS1070; pS1071; pT1078; pS1081;
pT1085; pY1092; pS1104; pY1110; pY1125; pY1138; pT1141;
pS1162; pS1166; pY1172; pS1190; pT1191; pY1197
IPI00290337 Epidermal growth factor receptor 100000 91882 7.56 11 17 236/38 EQFIPPYVPR; APAPAPPGTVTQVDVR pS661; pS662; pS663; pS664; pS666; pS813; pS817 EGF receptor substrate which enhances EGF-dependent mitogenic
kinase substrate 8 signalling; binds multiple cellular targets
IPI00001477 Epithelial discoidin domain- 140000 101129 6.81 7 7 124/37 HNLGGNLGDPR; NCLVGENFTIK pY484; pY513; pY520; pY703; pY740; pY792; pY796; pY797; Involved in cell-cell interactions and recognition
containing receptor 1/DDR1/CAK pY869; pY881
IPI00014263* Eukaryotic translation initiation 25000 27385 7.37 5 17 44/35 FRDGPPLR; EALTYDGALLGDR; pY11; pY41; pY44; pY85; pY100; pS192; pT216; pS229 Initiation of protein translation; may stimulate the activities of EIF4A, EIF4B
factor 4H, isoform 1 LQLKPR; AYSSFGGGR and EIF4F during mRNA binding
IPI00220894* Eukaryotic translation initiation 25000 25242 6.67 5 17 44/35
factor 4H, isoform 2
IPI00005969 F-actin capping protein alpha- 40000 33073 5.45 7 24 170/36 ESCDSALR; LLLNNDNLLR pS9 F-actin capping protein; binds in a calcium-independent manner to fast
1/CAPZA1 growing ends of actin filaments to block subunit exchange; cannot sever
actin filaments like severin and gelsolin
IPI00007797 Fatty acid-binding protein, 140000 15033 7.05 2 17 94/36 ELGVGIALR; LVVECVMNNVTCTR pT2; pY130 High specificity binding to fatty acids particularly C18 chain length; may be
epidermal involved in keratinocyte differentiation
IPI00644231 FMR1 interacting protein 1, 180000 206806 6.41 27 20 560/37 LGTPQQIAIAR; YSNSEVVTGSGR pY108; pT1068 Unknown function but interacts with the small GTPase Rac1
cytoplasmic
IPI00012885 Focal adhesion kinase 1 130000 119234 6.61 31 22 360/37 GIGQVLPTHLMEER; pT13; pS16; pS17; pT18; pT20; pT24; pS29; pY148; pY155; Non-receptor protein-tyrosine kinase implicated in cell motility, adhesion,
LVNGTSQSFIIRPQK pY347; pT386; pS390; pS392; pT394; pY397; pT406; pY407; proliferation and apoptosis signalling
pY441; pT503; pS517; pS568; pY570; pT575; pY576; pY577;
pS580; pT700; pS705; pS708; pS716; pY720; pS722; pS726;
pS732; pS766; pT805; pS840; pS843; pS850; pY861; pS887;
pS890; pS892; pS893; pS897; pY898; pS910; pT914; pY925;
pY1007
IPI00219221 Galectin 7 140000 14944 7.79 4 28 78/38 GNFHAVYR; LLETECPQYIR - Pro-apoptotic protein that functions intracellularly upstream of JNK
activation and cytochrome c release; involved in cell-cell and/or cell-matrix
interactions
IPI00304925* HSP70-1 65000 70026 5.31 11 18 233/37 ITITNDKGR; AQIHDLVLVGGSTR pY41; pY107; pS153; pT222; pT226; pS362; pY371; pT502; Protein folding, chaperone, stress response
pY525; pY611; pS631; pS633
IPI00514377* HSP70-1A 65000 69995 5.29 11 18 233/37
IPI00643932 HSP70-1B 65000 70267 5.48 6 9 63/36 TTPSYVAFTDTER; LLQDFFNGR - Protein folding, chaperone, stress response
IPI00301277 HSP70-1L 65000 70376 5.82 7 11 196/37 ITITNDKGR; AKIHDIVLVGGSTR - Protein folding, chaperone, stress response
IPI00339269 HSP70-6 65000 71029 6.01 4 6 107/37 ITITNDKGR; TTPSYVAFTDTER - Protein folding, chaperone, stress response
IPI00003865 HSP71 65000 70899 5.16 15 22 345/37 SQIHDIVLVGGSTR; NTTIPTK pY41; pY107; pS153; pT222; pT226; pS362; pY371; pT502; Protein folding, chaperone, stress response
pY525; pY611; pS613; pS631; pS633; pS637; pS638; pT641
IPI00382470 HSP90 alpha2 100000 98114 4.82 2 4 40/38 TLTIVDTGIGMTK; GVVDSEDLPLNISR pS353; pS374; pS385 Protein folding, chaperone, stress response
IPI00003362 HSPA5/GRP78/BiP 65000 72334 4.8 7 13 90/37 VEIIANDQGNR; ITPSYVAFTPEGER pT37; pT203; pT229; pY466 Protein folding; molecular chaperone
IPI00220845* Integrin beta-4A 180000 198522 5.74 6 3 126/37 LVFSALGPTSLR; HVTQEFVSR; pS7; pS21; pS368; pS377; pT383; pS387; pS821; pT829; pT837; Adhesion receptor; receptor for laminin; plays a critical structural role in the
VAPGYYTLTADQDAR; MLLIENLR pY855; pT886; pY920; pY921; pT973; pS1000; pS1084; pY1189; hemidesmosome of epithelial cells
pY1199; pY1207; pT1246; pY1257; pT1297; pT1318; pS1345;
pS1356; pS1360; pS1364; pY1399; pT1401; pS1403; pS1404;
pS1428; pT1434; pS1457; pT1460; pY1469; pS1474; pT1487;
pY1492; pS1494; pY1510; pT1514; pS1515; pS1517; pS1518;
pT1524; pT1530; pY1564; pY1596; pS1653; pT1688; pS1707;
pY1712; pS1762; pT1765; pT1766
IPI00220846* Integrin beta-4B 180000 204230 5.9 6 3 126/37
IPI00027422* Integrin beta-4C 180000 205729 5.74 6 3 126/37
IPI00220847* Integrin beta-4D 180000 197918 5.76 6 3 126/47
IPI00554711 Junction plakoglobin/Desmoplakin 80000 81500 6.33 14 20 189/38 LLNDEDPVVVTK; TMQNTSDLDTAR pY19; pY21; pY73; pY132; pT140; pS181; pT195; pT428; pT461; Junctional plaque protein; plays a central role in the structure and function
III pY478; pY548; pS594; pY642; pY658; pS663 of submembranous plaques; plakoglobin is present in both desmosomes
and intermediate junctions
IPI00219365 Moesin 80000 67690 6.32 10 13 79/37 KAPDFVFYAPR; pY115; pY269; pS383; pS467; pT468; pT557; pS575 Connects major cytoskeletal structures to the plasma membrane
VTTMDAELEFAIQPNTTGK
IPI00019502 Myosin 9 180000 226535 5.29 13 6 101/37 AGVLAHLEEER; YEILTPNSIPK pS1712; pS1713; pS1942 Plays a role in cytokinesis and specialized functions such as secretion and
capping
IPI00031982* Nck-associated protein 1/NCKAP1 140000 128792 6.61 24 15 70/37 ASLGLADHR; LVVENVDVLTQMR pS4; pT18; pT29; pT142; pS148; pS171; pS899; pS1050; pS1065; Part of a lamellipodial complex that controls Rac-dependent actin
pY1116; pY1120 remodelling
IPI00409684* Nck-associated protein 1 isoform 140000 129433 6.23 24 15 70/37
2/ NCKAP1
IPI00045423 Partitioning-defective 3/Par3 180000 141732 7.36 6 5 114/38 GMIQLIVAR; SQEEVVSLLR pS25; pS143; pS144; pS383; pY388; pT481; pS485; pY489; Adapter protein involved in asymmetrical cell division and cell polarization
(isoforms 1-9 equally identified) pT579; pS692; pS695; pS715; pS717; pY719; pS720; pS728; processes; plays a central role in the formation of epithelial tight junctions
pS803; pS809; pS827; pS852; pS873; pS889; pS924; pY1080;
pY1127; pY1177; pY1244; pY1310; pY1321
IPI00220030* Paxillin-alpha 65000 60937 6.32 9 20 244/37 KPIAGQVVTAMGK; CYYCNGPILDK; pY118 pS19; pT29; pY31; pY40; pS83; pS84; pS85; pY88; pS90; pS91; Cytoskeletal protein involved in actin-membrane attachment at sites of cell
ELDELMASLSDFK; pT94; pS95; pS96; pS98; pS106; pS109; pY118; pS119; pS126; adhesion to the extracellular matrix (focal adhesion)
GSLCSGCQKPITGR; pS130; pT132; pT136; pS137; pS143; pS170; pS178; pY181;
VGEEEHVpYSFPNK; pT186; pS188; pT198; pS216; pS219; pS226; pS227; pS230;
pT236; pS244; pS250; pS258; pS261; pT263; pS272; pS274;
pT284; pS287; pS288; pS298; pS306; pT342; pS359; pY375;
pS380; pT396; pT401; pY434; pS455; pS479; pS499; pS502;
pT538
IPI00335634* Paxillin-beta 65000 65974 5.73 9 19 244/37
IPI00220031* Paxillin-gamma 65000 67679 5.94 9 18 244/37
IPI00024919 Peroxiredoxin 3/Thioredoxin- 30000 28017 7.67 7 26 151/36 DYGVLLEGSGLALR; - Redox regulation
dependent peroxide reductase NGGLGHMNIALLSDLTK
IPI00012066 Poly(rC) binding protein 2, 50000 38597 6.33 3 11 43/36 IANPVEGSTDR; IITLAGPTNAIFK pY178; pS183; pS184; pS185 Single-stranded nucleic acid binding protein that binds preferentially to oligo
isoform B /hnRNPE2 dC
IPI00181905 Protein KIAA1914/Actin filament- 100000 91300 4.97 5 7 76/37 AVTPASAPDCTPVNSATTLK; pY54; pY56; pY307; pY383; pY413; pY459 Unknown; contains 2 PH domains
associated protein 1-like 2 ITPMNADVIVLGLQSK
IPI00029702 PYK2/Protein tyrosine kinase 2 130000 115876 6.16 12 11 178/37 LIGSVDDLLPSLPSSSR; WMSPESINFR pS9; pT15; pS332; pS375; pS389; pS392; pS394; pS396; pS399; Involved in calcium-induced regulation of ion channels and activation of
beta pY402; pT409; pY440; pS530; pS571; pY579; pY580; pY683; MAP & JNK kinase signaling pathways; interacts with the SH2 domain of
pY699; pS718; pT751; pS752; pY756; pS758; pS762; pT765; Grb2; activation is highly correlated with stimulation of JNK
pY834; pS839; pT842; pT853; pY881
IPI00107831* Receptor-type protein tyrosine- 180000 211847 6.26 2 1 57/37 HVVDGISR; SDMGVGVFTPTIEAR pY1311 Cell adhesion receptor; possesses an intrinsic protein tyrosine phosphatase
phosphatase F precursor/LAR activity
IPI00465186* Receptor type protein tyrosine 180000 211553 6.02 2 1 57/37
phosphatase F
IPI00449906 Rho guanine nucleotide exchange 80000 73141 7.23 7 12 108/37 GDVIHVTR; MSGFIYQGK; pS71; pS340; pS525; pT526; pS579; pT583 RAC1 guanine nucleotide exchange factor (GEF); can induce membrane
factor 7 isoform 1/ARHGEF7 TDNSNNQLVVR; NAFEISGSMIER; ruffling
ELELQILTEAIR
IPI00007047 S100A8/Calgranulin A 140000 10835 7.07 3 32 82/36 GNFHAVYR; LLETECPQYIR - Ca2+ binding protien involved in cell cycle progression and differentiation;
marker for endometrial carcinoma
IPI00027462 S100A9/Calgranulin B 140000 13242 6.08 5 37 50/37 NIETIINTFHQYSVK; pT113 Ca2+ binding protien involved in cell cycle progression and differentiation;
VIEHIMEDLDTNADK marker for endometrial carcinoma
IPI00298347 SHPTP2 isoform 2/Tyrosine- 65000 68012 7.31 10 19 118/37 MVFQENSR; NILPFDHTR; pS28; pS34; pY62; pY63; pY66; pY279; pY304; pY327; pT415; Acts downstream of various receptor and cytoplasmic protein tyrosine
protein phosphatase non-receptor GVDGSFLARPSK; SGMVQTEAQYR; pY546; pT547; pS552; pY584; pS595 kinases in signal transduction
type 11
IPI00007188 SLC25A5 protein/ADP/ATP 30000 40041 10.2 2 6 46/35 LLLQVQHASK; DFLAGGVAAAISK - Catalyzes the exchange of ADP and ATP across the mitochondrial inner
translocase 2 membrane
IPI00216219 Tight junction protein ZO-1 180000 195841 6.84 23 13 274/37 IVESDVGDSFYIR; VNNVDFTNIIR pS125; pS131; pY132; pS141; pS144; pS151; pS160; pS162; Tight junction assembly and stabilisation
pS166; pS168; pS175; pS178; pS212; pS275; pS277; pS280;
pS284; pS290; pS294; pS297; pS300; pS311; pS323; pS326;
pS329; pS337; pS347; pS353; pT354; pT363; pT379; pS381;
pY389; pS402; pS432; pT476; pY588; pT589; pS617; pS622;
pT648; pT738; pT772; pY830; pT868; pS877; pS878; pS885;
pS886; pT894; pY895; pY898; pS899, pS912; pS926; pS927;
pS933; pT965; pS968; pS973; pT976; pS1025; pY1059; pY1066;
pY1100; pS1102; pS1111; pS1116; pY1165; pY 1191; pY1195;
pY1199; pY1324; pY1346; pY1354; pY1355; pS1366; pY1435;
pS1451; pY1524; pY1532; pS1534; pS1545; pS1577; pS1617;
pS1619; pS1655
IPI00003843 Tight junction protein ZO-2 180000 133973 7.43 33 24 449/38 ETPQSLAFTR; HPDIYAVPIK; pY1118 pS16; pT91; pS130; pS163; pS168; pS170; pS174; pS186; pS192; Plays a role in tight junctions and adherens junctions
GEMVTILAQSR;HPDIpYAVPIK; pS196; pS200; pT212; pS216; pS244; pY257; pY261; pY265;
DGNLHEGDIILK; AFEVMDEFDGR; pS266; pS279; pS284; pS286; pS294; pS296; pT388; pS394;
pS398; pS400; pS415; pY423; pS424; pY426; pY428; pS430;
pS431; pS432; pS440; pS441; pT455; pY506; pY574; pT585;
pS590; pS702; pT707; pS711; pS713; pT760; pS767; pS770;
pT771; pT778; pT831; pS886; pY911; pS913; pS920; pT925;
pY932; pT933; pS961; pS966; pS978; pS986; pY1007; pS1048;
pS1053; pT1054; pS1067; pS1068; pY1118; pT1124; pT1131;
pT1135; pY1157; pS1159; pY1166; pS1171; pS1174
IPI00073096* Tripartite motif protein 29 65000 65807 7.16 3 5 70/37 TSYQPSSPGR; pS21; pS23; pY93; pS104; pY106; pY471; pY474; pT476; pS488; Complements the radiosensitivity defect of an ataxia telangiectasia
alpha/TRIM29 SLGSALKPGEGR;EQDAVDQVK pS489 fibroblast cell line; colocalizes with intermediate filaments
IPI00232492* Tripartite motif protein 29 65000 63803 6.54 3 5 70/37
beta/TRIM29
IPI00007750 Tubulin alpha-1A 50000 49925 4.69 10 23 147/37 LISQIVSSITASLR; NLDIERPTYTNLNR pS48; pT51; pY103; pY210; pT223; pY224; pT225; pT271; pY272; The major constituent of microtubules; GTP binding
pY282; pY319; pS340; pY357; pS439; pY440
IPI00180675* Tubulin alpha-3 50000 50136 4.7 6 13 140/35 EDAANNYAR; EIIDLVLDR pS48; pT51; pY103; pY210; pT223; pT225; pY357 The major constituent of microtubules; GTP binding
IPI00387144* Tubulin alpha-ubiquitous chain 50000 50120 4.94 6 13 140/35 pS48; pT51; pY103; pY210; pT223; pY224; pT225; pY272; pY319;
pT334; pY357; pS379; pY432; pS439; pY451
IPI00218343 Tubulin alpha-6 50000 49896 4.73 13 32 297/37 TIGGGDDSFNTFFSETGAGK; pS48; pT51; pY103; pY210; pT223; pY224; pT225; pY272; pY319; The major constituent of microtubules; GTP binding
AVFVDLEPTVIDEVR pT334; pY357; pS439; pY449
IPI00011654 Tubulin beta-2 50000 49671 4.52 10 22 161/37 IREEYPDR; FPGQLNADLR pY36; pY106; pT107; pT136; pS138; pT143; pS145; pS172; The major constituent of microtubules; GTP binding
pT274; pS275; pS278; pY340; pS382
IPI00179330 Ubiquitin and ribosomal protein 140000 17905 10.22 3 16 59/38 ESTLHLVLR; TITLEVEPSDTIENVK pS65 Ribosome component synthesised as C-terminal extension of ubiquitin
S27a
IPI00022774 VCP/Transitional endoplasmic 100000 89192 4.89 36 26 397/38 VINQILTEMDGMSTK; GDDLSTAILK pS2; pS6; pT13; pT167; pY172; pY243; pS275; pT346; pS351; Involved in the formation of the transitional endoplasmic reticulum required
reticulum ATPase pT435; pS456; pS458; pT508; pS540; pS554; pT605; pT612; for the transfer of membranes from the endoplasmic reticulum to the Golgi
pT622; pT678; pS701; pS704; pS745; pS747; pT760; pS774; apparatus
pS783; pS786; pY795; pT790; pS793; pY795; pT796; pY804
IPI00479359 Villin2/Ezrin 80000 69268 6.17 22 31 371/38 KAPDFVFYAPR; SQEQLAAELAEYTAK - Connects major cytoskeletal structures to the plasma membrane
IPI00472164 Wiskott-Aldrich syndrome protein 65000 54284 5.16 4 7 121/37 VSSLAER; DVVGNDVATILSR pT29; pT32; pT137; pY150; pS482; pS484; pS488; pS489 Downstream effector molecule involved in the transmission of signals from
family member 2/WAVE2 tyrosine kinase receptors and small GTPases to the actin cytoskeleton;
binds actin, Arp2/3 complex, NCKAP1 and BAIAP2.
Supplemental Table 1. Proteins and phosphorylation sites identified in large-scale a-phosphotyrosine IPs from H2O2-treated HB4a cells. H2O2-induced tyrosine phosphorylated proteins were purified and identified by LC-MS/MS. The table shows all identified proteins organized by name with IPI
accession number, approximate gel-based MW, predicted MW, number of peptides matching the database sequence, sequence coverage (%), Mascot database search score (denominator is score at P =0.05), at least two identified peptides, identified phosphorylation sites, known phosphorylation sites from the
Swissprot, PHOSIDA and PhosphoSite databases and molecular function. Gray shaded cells indicate proteins where gel-based and predicted MWs were not in agreement. *Proteins of overlapping sequence identity (e.g. splice isoforms, processed precursors) that could not be discriminated using identified peptides.