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1 Biotechnology International, Vol 3, 2010 ISSN 0974-1453 Online www.bti.org.in Candida albicans Vaccines ; pp. 4- 17 Aditi Grover, B. S. Bhandari, Nishant Rai*, Pramesh C Lakhera Since most fungal infections occur in immunocompromised patients, the generation of tools relying on host immunity for effectiveness is a notable challenge. Nevertheless, with improved knowledge of the host-fungus relation, and the spectacular advances in genome sequencing, genetic engineering, and proteomics, strong progress in fungal vaccine research has been made. Some vaccines induce the generation of directly antifungal antibodies; others are protective in animals carrying major risk factors for fungal infections. Together with demonstrated efficacy of various antibodies in passive vaccination approaches, there is growing confidence in the future availability of safe and efficacious immunological tools to combat deadly microbes in a weak host. Purification and activity of phage induced lysin against mastitogenic strains of Staphylococcus aureus ; pp.18-25 Abhishek * Mayank Rawat and Anil Mishra A protein/proteins preparation from a phage lysate of a mastitogenic Staphylococcus aureus isolate was purified by physico-chemical methods. The protein/s were found to possess a greater lytic range against different staphylococcal isolates and demonstrated significantly higher lytic efficacy in presence of raw cow -milk than its/their inducing phage. The results indicate good potential of the phage lysins to be used as therapeutic agents against mastitis. Prediction of MHC-I binding epitopes in a gene fragment encoding 183 amino acids of Mycobacterium bovis Bacillus Calmette-Guerin (BCG) strain ; Pp 29-32. Ramesh.D., adeesh .E.M1., R . Deb. B. Sailo., V. K. Saxena., V.G Joshi and Yosef .D In this study, in silico prediction of nanomeric epitopes of Mycobacterium bovis BCG str. Tokyo 172 was explored, and analyzed for CD8+ T cell binding ability based on their predicted peptide scores for their respective MHC-I specific alleles Amplification and cloning of streptokinase gene in pTargeT mammalian expression vector Pp 33-49 Soni Gangwar, Pramesh C Lakhera, Anant Rai 2 Streptokinase gene of Streptococcus pyogenes was successfully amplified by hot start PCR using SK gene specific forward and reverse primers. The PCR product was 1353bp long and a clear compact thick band was observed. When this ligation product was used for transforming E.coli DH5α, large numbers of white colonies were observed. A total of 13 clones were screened by colony PCR and clones 1,2,3,5,7,8,10,11 and 12 showed presence of insert. These clones were also tested in RE analysis and it was found that clone 1 had no insert, clones 2,3,5,7,8 had insert in wrong orientation and clone 10 was confirmed to have gene in right orientation. Modeling of streptokinase protein ; pp.53 - 61 Soni Gangwar, Pramesh C Lakhera, Anant Rai Modeling of SK protein were observed using spdbv software and further analysed using Rasmol software to visualize the 3-D structure of the protein which generated backbone, structure color group, ball and stick, space fill, shapely, strand, and wireframe and ribbon model structure. These observations provided valuable information on 3-D structure, surface domains and molecular structure of the SK protein. Modeling of SK protein was also done using DNAstar protein software and different structural details were visualised Cloning of virulent rabies virus glycoprotein gene in replicase based vector ; pp. 62 - 70 Soni Gangwar, Anant Rai, Praveen K Gupta, Ankur Saxena, Nishant Rai Rabies virus virulent strain G gene was released from recombinant pTargeT vector and recloned in pSinCMV vector at StuI site using blunt end ligation.The clone containing gene in right orientation was confirmed by restriction enzyme digestion, PCR amplification using Forward gene specific primer and BGH primer as reverse primer, and sequencing of the cloned gene using BGH primer.
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