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Biotechnology International, Vol 3, 2010
       ISSN 0974-1453 Online

Candida albicans Vaccines ; pp. 4- 17
Aditi Grover, B. S. Bhandari, Nishant Rai*, Pramesh C Lakhera

Since most fungal infections occur in immunocompromised patients, the generation of tools relying on
host immunity for effectiveness is a notable challenge. Nevertheless, with improved knowledge of the
host-fungus relation, and the spectacular advances in genome sequencing, genetic engineering, and
proteomics, strong progress in fungal vaccine research has been made. Some vaccines induce the
generation of directly antifungal antibodies; others are protective in animals carrying major risk factors
for fungal infections. Together with demonstrated efficacy of various antibodies in passive vaccination
approaches, there is growing confidence in the future availability of safe and efficacious immunological
tools to combat deadly microbes in a weak host.

Purification and activity of phage induced lysin against mastitogenic strains of
Staphylococcus aureus ; pp.18-25
Abhishek * Mayank Rawat and Anil Mishra

A protein/proteins preparation from a phage lysate of a mastitogenic Staphylococcus aureus isolate was
purified by physico-chemical methods. The protein/s were found to possess a greater lytic
range against different staphylococcal isolates and demonstrated significantly higher lytic
efficacy in presence of raw cow -milk than its/their inducing phage. The results indicate
good potential of the phage lysins to be used as therapeutic agents against mastitis.

Prediction of MHC-I binding epitopes in a gene fragment encoding 183 amino acids of
Mycobacterium bovis Bacillus Calmette-Guerin (BCG) strain ; Pp 29-32.
Ramesh.D., adeesh .E.M1., R . Deb. B. Sailo., V. K. Saxena., V.G Joshi and Yosef .D

In this study, in silico prediction of nanomeric epitopes of Mycobacterium bovis BCG str. Tokyo
172 was explored, and analyzed for CD8+ T cell binding ability based on their predicted peptide
scores for their respective MHC-I specific alleles

Amplification and cloning of streptokinase gene in pTargeT mammalian expression vector
Pp 33-49
Soni Gangwar, Pramesh C Lakhera, Anant Rai

Streptokinase gene of Streptococcus pyogenes was successfully amplified by hot start PCR using
SK gene specific forward and reverse primers. The PCR product was 1353bp long and a clear
compact thick band was observed. When this ligation product was used for transforming E.coli
DH5α, large numbers of white colonies were observed. A total of 13 clones were screened by
colony PCR and clones 1,2,3,5,7,8,10,11 and 12 showed presence of insert. These clones were
also tested in RE analysis and it was found that clone 1 had no insert, clones 2,3,5,7,8 had insert
in wrong orientation and clone 10 was confirmed to have gene in right orientation.
Modeling of streptokinase protein ; pp.53 - 61
Soni Gangwar, Pramesh C Lakhera, Anant Rai

Modeling of SK protein were observed using spdbv software and further analysed
using Rasmol software to visualize the 3-D structure of the protein which generated backbone,
structure color group, ball and stick, space fill, shapely, strand, and wireframe and ribbon model
structure. These observations provided valuable information on 3-D structure, surface domains
and molecular structure of the SK protein. Modeling of SK protein was also done using DNAstar
protein software and different structural details were visualised

Cloning of virulent rabies virus glycoprotein gene
in replicase based vector ; pp. 62 - 70
Soni Gangwar, Anant Rai, Praveen K Gupta, Ankur Saxena, Nishant Rai

Rabies virus virulent strain G gene was released from recombinant pTargeT vector
and recloned in pSinCMV vector at StuI site using blunt end ligation.The clone containing gene
in right orientation was confirmed by restriction enzyme digestion, PCR amplification using
Forward gene specific primer and BGH primer as reverse primer, and sequencing of the cloned
gene using BGH primer.

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