Figures legends: Supplemental S1, S2
Figure S1
CrtI expression levels and total carotenoid content of transgenic BY2 cells.
A: The CrtI expression levels of eight transgenic lines (o1, o2, o3, o5, o6-o8, o10) were
determined using TaqMan Real-Time-PCR with RNA preparations form cells harvested 4
days after subculture as detailed in the Method section. Each bar represents a pool of six RNA
preparations, from 6 separate harvests. The line with the strongest CrtI expression was set to
1.
B: Total carotenoid content of eight transgenic lines and wild-type cells was determined using
HPLC. Each bar respresents the mean of 6 measurements.
Figure S2
Expression levels measured by TaqMan Real-Time-PCR of selected intrinsic tobacco
carotenoid biosynthesis genes in BY2 cells. The expression levels of phytoene-synthase
(PSY), phytoene-desaturase (PDS), -carotene-desaturase (ZDS), lycopene-cis-trans-
isomerase (ISO) and lycopene--cyclase (LCY) were assayed using RNA preparations from
cells harvested 4 days after subculture as detailed in the Method section. Each bar represents a
pool of six RNA preparations, from 6 separate harvests. White bars, wild-type; light grey
bars, line o1; dark grey bars, line o2; black bars, line o3.