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Full-scale y-axis 149 mm



NormFact NormFact determined

Detector AngDeg Millimeters Scale Signal by Wyatt

7 44.5 90 0.5 0.302 0.967 0.97

8 54 100 0.5 0.336 0.870 0.869

9 64.9 97.4 0.5 0.327 0.893 0.888

11 90 87 0.5 0.292 1.000 1

12 102.9 93.1 0.5 0.312 0.934 0.921

13 115.1 109 0.5 0.366 0.798 0.791

14 126 119.9 0.5 0.402 0.726 0.722

15 135.5 51.8 1 0.348 0.840 0.831

16 143.9 78 1 0.523 0.558 0.548





Divide intensity by

intensity at 90

intensity by

intensity at 90

Full-scale Y-axis



c1 c2

Norm X

Detector AngDeg q2/cm-2 Norm DAWN Scale/Voltsmm

7 44.5 1.00E+10 0.967 1.483E-05 1 62.5 70

8 54 1.44E+10 0.870 1.335E-05 1 68.4 76

9 64.9 2.01E+10 0.893 1.370E-05 1 60.6 95

11 90 3.49E+10 1.000 1.534E-05 1 58.2 66

12 102.9 4.27E+10 0.934 1.433E-05 1 63.3 71.2

13 115.1 4.97E+10 0.798 1.224E-05 1 73.8 83

14 126 5.54E+10 0.726 1.113E-05 1.25 64 72.4

15 135.5 5.98E+10 0.840 1.288E-05 1.5 46.2 52.5

16 143.9 6.31E+10 0.558 8.555E-06 2 53.5 60



dn/dc in cm^3/g 0.198

With the exception of a few points, this looks OK. The polymer i

Opt.Const mol-cm2-g-2 2.836E-07

too small to size by SLS.

ScaleConst 0.000E+00

1.4E-04





Get intercepts visually! 1.2E-04

conc q=0 intrcpts

0.00103 4.7

0.00129 5.3 1.0E-04

0.00167 6.15

0.00209 7

Kc/R (mol/g)









0.00247 7.65 8.0E-05

0.00489 12.79



6.0E-05

Conc Dependence

14 y = 2081.4x + 2.6027

4.0E-05

12 c=0 guide lines q=0 guidelines

x

2.0E-05

10 1.00E+10 0 ybottom 0 0.00

1.00E+10 1.00E-04 ytop 0 1.00E-04

Kc/R x 10^5









8 1.44E+10

0.0E+00 0 ybottom 0 0.00

1.44E+10

0.00E+00 ytop

1.00E-04 2.00E+10 0 1.00E-04

4.00E+10

6 2.01E+10 0 etc 0.00

0 +kscale*c (both in cm^

q2

2.01E+10 1.00E-04 0 1.00E-04

4 3.49E+10 0 0 0.00

3.49E+10 1.00E-04 0 1.00E-04

2 4.27E+10 0 0 0.00

4.27E+10 1.00E-04 0 1.00E-04

0 4.97E+10 0 0 0.00

-0.001 0 0.001 0.002 0.003 0.004 0.005 0.006

-0.001 0 0.001 0.002 0.003 0.004 0.005

4.97E+10 0.006

1.00E-04 0 1.00E-04

5.54E+10 0

5.54E+10 1.00E-04

5.98E+10 0

5.98E+10 1.00E-04

6.31E+10 0

6.31E+10 1.00E-04

149 mm DAWN const : 1.534E-05



c3 c4 c5 c6 c1 g/mL c2 g/mL c3 g/mL c4 g/mL c5 g/mL c6 g/mL

0.00103 0.00129 0.00167 0.00209 0.00247 0.00489

Rayleigh factor

76.9 86 92 108 6.220E-06 6.966E-06 7.653E-06 8.559E-06 9.156E-06 1.075E-05

85 95 102 119 6.127E-06 6.807E-06 7.613E-06 8.509E-06 9.091E-06 1.066E-05

93.5 93 100 118 5.573E-06 8.736E-06 8.598E-06 8.552E-06 9.196E-06 1.081E-05

73.7 82.3 89 105 5.992E-06 6.795E-06 7.588E-06 8.473E-06 9.163E-06 1.083E-05

79.4 89 96 114 6.090E-06 6.850E-06 7.639E-06 8.562E-06 9.236E-06 1.097E-05

93.2 104 112 134 6.064E-06 6.820E-06 7.659E-06 8.546E-06 9.203E-06 1.099E-05

81 91 98 118 5.976E-06 6.761E-06 7.564E-06 8.497E-06 9.151E-06 1.097E-05

59 66 71 85 5.991E-06 6.808E-06 7.651E-06 8.559E-06 9.247E-06 1.102E-05

67 76 82 98 6.144E-06 6.890E-06 7.694E-06 8.727E-06 9.416E-06 1.125E-05



Kc/R

nts, this looks OK. The polymer is

4.696E-05 5.252E-05 6.189E-05 6.926E-05 7.651E-05 1.290E-04

4.768E-05 5.375E-05 6.221E-05 6.966E-05 7.706E-05 1.301E-04

5.242E-05 4.188E-05 5.509E-05 6.931E-05 7.618E-05 1.284E-04

4.875E-05 5.384E-05 6.242E-05 6.996E-05 7.645E-05 1.281E-04

4.797E-05 5.341E-05 6.200E-05 6.923E-05 7.585E-05 1.265E-04

4.817E-05 5.364E-05 6.184E-05 6.936E-05 7.612E-05 1.261E-04

4.888E-05 5.412E-05 6.262E-05 6.976E-05 7.655E-05 1.264E-04

4.876E-05 5.374E-05 6.190E-05 6.925E-05 7.576E-05 1.258E-04

4.755E-05 5.310E-05 6.156E-05 6.792E-05 7.440E-05 1.232E-04



q2+kscale*c c1

1.00E+10 1.00E+10 1.00E+10

c2 1.00E+10 1.00E+10 1.00E+10

1.44E+10 1.44E+10 1.44E+10

c3 1.44E+10 1.44E+10 1.44E+10

2.01E+10 2.01E+10 2.01E+10 2.01E+10 2.01E+10 2.01E+10

c4

3.49E+10 3.49E+10 3.49E+10 3.49E+10 3.49E+10 3.49E+10

4.27E+10 4.27E+10 c5

4.27E+10 4.27E+10 4.27E+10 4.27E+10

4.97E+10 4.97E+10 4.97E+10

c6 4.97E+10 4.97E+10 4.97E+10

5.54E+10 5.54E+10 5.54E+10 5.54E+10

SampleGuideLine 5.54E+10 5.54E+10

5.98E+10 5.98E+10 5.98E+10 5.98E+10 5.98E+10 5.98E+10

6.31E+10 6.31E+10 6.31E+10 6.31E+10 6.31E+10 6.31E+10









4.00E+10 6.00E+10 8.00E+10

kscale*c (both in cm^-2)





<<<<












Full-scale Y-axis 149 mm DAWN const :



c1 c2 c3 c4 c5 c6 c1 c2

Ang Norm / Scale/

Det. Deg q2/cm-2 Norm sin(q) Volts mm of signal from printouts Signal

7 44.5 1.00E+10 0.967 1.379 1 62.5 70 76.9 86 92 108 0.579 0.648

8 54 1.44E+10 0.870 1.075 1 68.4 76 85 95 101.5 119 0.494 0.549

9 64.9 2.01E+10 0.893 0.986 1 60.6 95 93.5 93 100 118 0.401 0.629

11 90 3.49E+10 1.000 1.000 1 58.2 66 73.7 82.3 89 105 0.391 0.443

12 103 4.27E+10 0.934 0.959 1 63.3 71.2 79.4 89 96 114 0.407 0.458

13 115 4.97E+10 0.798 0.881 1 73.8 83 93.2 104 112 134 0.437 0.491

14 126 5.54E+10 0.726 0.897 1.25 64 72.4 81 91 98 118 0.482 0.545

15 136 5.98E+10 0.840 1.198 1.5 46.2 52.5 59 66 71.3 85 0.557 0.633

16 144 6.31E+10 0.558 0.947 2 53.5 60 67 76 82 98 0.680 0.762





This sheet was an attempt to fake out the GuiDe program. So far, I

have not succeeded at this. Maybe something wrong with the

baseline subtraction???

1.53E-05



c3 c4 c5 c6





0.712 0.796 0.852 1.000

0.613 0.686 0.733 0.859

0.619 0.616 0.662 0.778

0.495 0.552 0.597 0.706

0.511 0.573 0.618 0.733

0.551 0.615 0.663 0.791

0.609 0.685 0.737 0.884

0.712 0.796 0.860 1.025

0.851 0.966 1.042 1.245





program. So far, I

ng wrong with the

0.02137









0.384615



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