Fingerswitch for Bipolar Forceps by cuiliqing

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									    TEAM MEMBERS

Dan Carlson    Team Leader
Dave Ugai      Communicator
Erik Bieging   BWIG/BSAC
“THE SOLUTIONATORS”




From left: Dave Ugai, Erik Bieging, and Dan Carlson
       CLIENTS
  Jinling Wang, Ph.D.
    Research Associate
  Department of Physiology

Gail A. Robertson, Ph.D.
    Associate Professor
  Department of Physiology
       ADVISOR
Professor John G. Webster, Ph.D.
             ABSTRACT
The objective of the project is to design an
automated system of valves for a solution
delivery system to be used in patch clamp
research. The current system has two to eight
solution reservoirs suspended in the air above
a manifold. The manifold allows the
researcher to manually control which solution
is being delivered. This is a tedious process
for the researcher, so an automated system is
desired.
PROBLEM STATEMENT

   The goal of this project was to
develop a computer-automated
system for delivering solutions to a
patch clamp for physiological
laboratory research.
              PATCH CLAMP
   Method used to study
    single cells
   HERG potassium
    channel is critical in
    repolarization of the
    heart’s action potential
   Long QT syndrome is a
    disease that can lead to
    cardiac arrhythmia or
    sudden death                  MICRO PIPET OVER ION CHANNEL
                               http://www.iac-usnc.org/Methods/wholecell/equipment.html
CURRENT SETUP
       Solutions are fed
       to a manifold and
       as the orientation
       of the multi-
       position stop-cocks
       is changed, the
       solution being
       delivered changes
       MOTIVATION

•The current method for switching
solutions is manually changing the
alignment of valves on a manifold
•This is a tedious process that
wastes valuable research time
 CLIENT REQUIREMENTS
 Must  reliably open and close valves on
  command from the computer
 Compatible with up to eight feeds of
  medical tubing
 Must generate minimal electrical and
  mechanical noise (so as not to disturb
  delicate physiological research)
        VARIABLE DESIGN
          COMPONENTS
1.   Valves: solenoid or solenoid pinch
     valve (both normally closed)

2.   Valve Controller: Valvelink8,
     Controlled 8 relay driver, Manually
     assembled controller
    SOLENOID VALVES




When current runs through the solenoid the
plunger is drawn up and the valve opens
 SOLENOID PINCH VALVES




The spring loaded plunger pinches the tubing, and
when current runs through the solenoid, the plunger
is pulled back.
   DECISION MATRIX
           Valvelink8 R85PRO Manually
                             assembled
Price      1          5      9

Simplicity 10        8       4

Reliability 10       9       7

Total      21        24      20
FINAL DESIGN COMPONENTS
1. R85PRO relay driver (1)
2. 1/8 inch solenoid valves, normally closed (8)
3. RSIO Adapter (1)
4. Serial cable (1)
5. 8-into-1 Teflon micro manifold with flow control
   (1)
6. NPT Male Pipe adapter (1/8” x 1/16”) (16)
    MOUNTED DESIGN
               1. R85PRO Driver
3              2. Solenoid valves

         1     3. RSIO adapter


               The design was
               mounted on foam
               pads in a plastic
               box to reduce the
               mechanical noise
         2     produced by the
               valves
       FUTURE WORK

 Install   device in client’s laboratory

 Configure     with pClamp data acquisition
software

 Provide    instruction to client on usage
              REFERENCES
   Jack Commins, sales representative, AutoMate
    Scientific
   L. Burke O’Neal, Biomedical Engineering Instrument
    Innovator, UW Madison
   Ryan Sheldon, owner, National Control Devices,
    controlanything.com
   James A. Sullivan, author, www.cellsalive.com
   American Heart Association, website,
    www.americanheart.org

								
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