Assay RH Vitamin B2 2

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					 The Rotherham NHS Foundation Trust                Laboratory Medicine: Biochemistry
 File name: Assay RH Vitamin B2 2.0                Document type: Information


                                   Vitamin B2
References:              CPA standard G5 Clinical advice and interpretation
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Location of copies       Web based only



VITAMIN B2 (RIBOFLAVIN)

REFERENCE RANGES

Urine > 24 nmol/mmol creatinine                           Screening test for deficiency

Whole blood (as FAD) = 220-750 nmol/L (Adult)             Follow up for monitoring

New ref range for whole blood Vit B2 effective from 25.09.02 - because of new
derivitisation procedure.

SPECIMEN REQUIREMENTS

Early morning urine protected from light. Freeze immediately after collection. Send to
lab by first class post.

EDTA or lithium heparin whole blood protected from light. Minimum volume 200ul.
Freeze immediately after collection. Send to lab by first class post.

METHOD

HPLC. Mix sample and internal standard with extraction buffer and precipitation reagent
and incubate. Add stabilisation reagent. Inject 50ul into HPLC. Riboflavin (as
flavinadenine dinucleotide, FAD) is detected using the fluorimetric detector (excitation
465nm, emission 525nm). Limit of detection 59 nmol/L. Linear to 2350 nmol/L.

INTERFERENCES

None listed




Edition No: 2.0                             Page 1 of 3
Author: Dr Rob Lord                         Authorised by: Dr Rob Lord
Issue date: 15.03.10                        Location: www.clinbiochem.info
 The Rotherham NHS Foundation Trust                   Laboratory Medicine: Biochemistry
 File name: Assay RH Vitamin B2 2.0                   Document type: Information



VALIDATION

Analytical

No limits

Clinical

All results pass into validation list.

INTERPRETATION

Riboflavin is a water soluble vitamin. It is found in vegetables. It is absorbed in the
intestine by a saturable transport system. Little is stored in the body and it is excreted in
the urine.

It is important as coenzymes flavinmononucleotide (FMN) and flavinadenine
dinucleotide (FAD) which have the ability to transport protons in the respiratory chain
and other redox reactions.

In blood ~95% occurs as FAD whilst riboflavin is the inactive form.

Deficiency of the vitamin impairs metabolism of carbohydrates, fatty acids, amino acids
and some other vitamins such as vitamin B6.


Deficiency

Clinical vitamin B2 deficiency is rare but it can manifest as

Anogenital inflammation
Atheroscerotic vascular changes
Angular stomatitis
Cheliosis
Glossitis
Magenta tongue

Seborrheic dermatitis and vascularisation of the cornea are also described.




Edition No: 2.0                                Page 2 of 3
Author: Dr Rob Lord                            Authorised by: Dr Rob Lord
Issue date: 15.03.10                           Location: www.clinbiochem.info
 The Rotherham NHS Foundation Trust             Laboratory Medicine: Biochemistry
 File name: Assay RH Vitamin B2 2.0             Document type: Information



Toxicity

Toxicity is not usually encountered.


Other methods to determine riboflavin levels include measurement of red blood cell
glutathione reductase.




Edition No: 2.0                          Page 3 of 3
Author: Dr Rob Lord                      Authorised by: Dr Rob Lord
Issue date: 15.03.10                     Location: www.clinbiochem.info

				
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Description: Exercise a strong muscle is every man's dream. People know that the body depends on muscle length intake of protein, but if you light up protein, no vitamin B2, more protein supplement is no good. This is because in many vitamins, vitamin B2 most likely to lack, it is involved in the synthesis of body protein metabolism, maintenance of the integrity of skin and mucous membranes of muscle development have an important role.