OIE PPCC LAB REPORT by liaoqinmei

VIEWS: 6 PAGES: 4

									                    OIE Reference Laboratory Reports
                                           Activities in 2010


   Name of disease (or topic) for                              Contagious bovine pleuropneumonia
 which you are a designated OIE
         Reference Laboratory:

                              Address of laboratory                Istituto G. Caporale Teramo, Via Campo Boario,
                                                                                  64100 Teramo, Italy


                                                 Tel.:                           (+39-0861) 33.22.05

                                                 Fax:                            (+39-0861) 33.22.51


                                     e-mail address:                                a.pini@izs.it

                                             website:                                www.izs.it


     Name of Head of Laboratory                                                Vincenzo Caporale
          (Responsible Official):

Name of OIE Reference Expert:                                                     Attilio Pini

      Name of writer of this report
         (if different from above):




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010                                        1
                                         Contagious bovine pleuropneumonia




             Part I: Summary of general activities related to the disease


1.   Test(s) in use/or available for the specified disease/topic at your laboratory

     Antigen for Complement fixation test, ELISA, PCR, Immunoblotting, Immunohistochemistry


                         Test                                 For                    Specificity               Total

                         CFT                               Antibody                     Group                    29

                        ELISA                              Antibody                     Group                    3

                      PCR-RFLP                              Antigen                    Specific                  36

            Real Time PCR to be validated                   Antigen                    Specific                  0

                   Immunoblotting                           Antigen                    MmmSC                     0

                Immunohistochemistry                        Antigen                     Group                    0



2.   Production and distribution of diagnostic reagents


                                            Amount supplied nationally                 Amount supplied to other
             Type of reagent
                                              (including for own use)                        countries

           Control positive serum                         40 ml                                      5 ml

           Control negative serum                         92 ml                                      2 ml

              Antigen for CFT                             12 ml                                     15 ml



                  Part II: Activities specifically related to the mandate
                              of OIE Reference Laboratories

3.   International harmonisation and standardisation of methods for diagnostic testing or the
     production and testing of vaccines

     –

4.   Preparation and supply of international reference standards for diagnostic tests or vaccines

     Available: positive reference serum for CFT. Supplied to OIE Members: 5ml


5.   Research and development of new procedures for diagnosis and control

     –

6.   Collection, analysis and dissemination of epizootiological data relevant to international disease
     control

     –




2                                              Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
                                               Contagious bovine pleuropneumonia




7.   Provision of consultant expertise to OIE or to OIE Members

     Participation to the Workshop organised by the Department of Veterinary Services, Zambian Ministry of
     Livestock and Fisheries Development for providing advice in the drawing up of the programme aimed to control
     and eradicates CBPP. Lusaka, Zambia 26-30 May 2010.


8.   Provision of scientific and technical training to personnel from other OIE Members

     1.   OIE Twinning Project between the Teramo Institute and the National Center for Animal and Plant Health
          (CENSA), Cuba.
               a.   One Veterinarian and one biologist were trained in the Laboratories of G. Caporale Institute on
                    anatomo-pathology of the disease, tissue sampling, microbiological culture, mycoplasma
                    identification by PCR. (March-April 2010)
               b.   A 3 days training course on “Mycoplasmas of Ruminants” was held on 12-14 October 2010 at
                    CENSA, La Habana, Cuba
     2.   OIE Twinning Project between the Teramo Institute and the Botswana National Veterinary Laboratory
          (BNVL)
               Three specialists from the Institute went to the BNVL for final training on diagnostic procedures and
               production of diagnostic reagents. (27 April – 21 May 2010)


9.        Provision of diagnostic testing facilities to other OIE Members

     –

10. Organisation of international scientific meetings on behalf of OIE or other international bodies

     Final Meeting on the occasion of the closure of the Twinning Project between the Institute and BNVL:
     Presentation of the BNVL as OIE Reference Laboratory candidate, Gaborone, Botswana. Co-organiser BNVL, 20
     participants, including representatives of Veterinary Services from South Africa, Namibia, Angola and Zambia.
     Conclusions & recommendations provided with the final report of the Twinning Project.


11. Participation in international scientific collaborative studies

     1.    Collaborative research study in progress: Identification of novel immunogenic proteins of MmmSC for the
           development of a vaccine able to induce a cell mediated immune response. Objective: development of a
           recombinant vaccine based on MmmSC target proteins; outcome: recombinant vaccine. In collaboration with
           the Moredum Research Institute, Bacterial Disease Division, Pentlands Science Park, Scotland, UK,
           International, the Livestock Research Institute of Nairobi (Kenia), the Central Veterinary Laboratory
           Windhoek, Namibia and the Central Veterinary Laboratory, Balmoral, Zambia.
     2.    Assessment of the regulatory T cell immune response in the course of Mycoplasma mycoides subsp mycoides
           SC experimental infections. The project aimed to: a) Investigate the immune pathogenic mechanisms
           involved in MmmSC infections; b) Analyse, quantitatively and qualitatively, the distribution of bovine B and
           T lymphocyte components, antibody isotypes and cytokine profile in order to understand humoral and cell-
           mediated reactivity during MmmSC infections. The project was run in collaboration with the International
           Livestock Research Institute of Nairobi, Kenya, VLA of Weybridge (UK), the Central Veterinary
           Laboratory, Balmoral, Zambia and the Central Veterinary Laboratory of Windhoek, Namibia. The project
           started in march 2007 and was completed in march 2010. The project highlighted some new aspects of CBPP
           pathogenic mechanisms.




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010                                         3
                                           Contagious bovine pleuropneumonia




12. Publication and dissemination of information relevant to the work of OIE (including list of
    scientific publications, internet publishing activities, presentations at international conferences)

         Presentations at international conferences and meetings

    1.    Jorg Jores, Anja Sterner-Kock, Wolfram Haider, Flavio Sacchini, Flora Mbithi, Jan Naessens 2010. Role of
          Acquired Immunity in Protection of Cattle against Contagious Bovine Pleuropneumonia. Abstract book pag.
          115, 18th Congress of the International Organization for Mycoplasmology, July 11-16, Chianciano Terme –
          Siena.

    2.    Flavio Sacchini, Enzo Langella, Mirella Luciani, Antonella Ciarelli, Romolo Salini, Attilio Pini, Jörg Jores &
          Jan Naessens 2010. Dynamics of TNF-α, IL-4, IFN-γ and IL-10 In MmmSC-Infected Cattle Depleted Or
          Non-Depleted For CD4+ T Lymphocytes. Abstract book pag. 29, 18th Congress of the International
          Organization for Mycoplasmology, July 11-16, Chianciano Terme – Siena.

         Scientific publications in peer-reviewed journals

    Anna Rita D’Angelo, Flavio Sacchini,Tiziana Di Febo,Vincenzo Langella, Andrea Di Provvido, Gabriella Di
    Francesco & Attilio Pini. 2010. Effects of immune serum on macrophage cell cultures infected with MmmSC:
    morphological scanning by electron microscopy. Veterinaria Italiana 46 (4), 389-396.


13. Inscription of diagnostic kits on the OIE Register

    i)    Did you participate in expert panels for the validation of candidate kits for inscription on the
          OIE Register? If yes, for which kits?
          –

    ii)   Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
          which kits?
          –

                                                  ______________




4                                                Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010

								
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