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					List of Antibodies tested on mouse tissue samples
Antigen                           Antigen Species                   Antibody type            Antibody Species Provider                                          Product No.   Batch          Antigen retrieval
15-lipoxygenase                   Human                             monoclonal, 061402DM     Rabbit           Oxford Biomedical Research                        LX25                         NO
Abcc6                             Mouse                             polyclonal               Goat            Santa Cruz                                         sc-5787       H3104
Acta2                                                               monoclonal, 1A4          Mouse           Sigma                                              A2547 1A4     037K4751
a-gp27 Gag                        Viral (MMTV)                      monoclonal, Blue-7-1     Mouse           The University of Chicago, Dr. Tatyana Golovkina                                Citrate (5 cycles)
a-gp36 Env                        Viral (MMTV)                      monoclonal, Blue 6-1     Mouse           The University of Chicago, Dr. Tatyana Golovkina                                NO
a-gp52 Env                        Viral (MMTV)                      monoclonal, Black 6-5D   Mouse           The University of Chicago, Dr. Tatyana Golovkina                                NO
Alpha actinin                     Chicken                                                    Rabbit          Sigma                                              A2543         78F-4865
Alpha catenin                     Human (recombinant)                                        Rabbit          Chemicon                                           AB19021                      Citrate (5 cycles)
Alpha catenin                     Human/Mouse (synthetic peptide)                            Rabbit          Sigma                                              C2081                        Citrate (5 cycles)
Alpha SHP-1                                                         monoclonal, D-11         Rabbit          Santa Cruz                                         sc-7289
Alpha smooth muscle actin         Human (synthetic peptide)         monoclonal, 1A4          Mouse           Sigma                                              A2547                        NO
Alpha-fetoprotein                 Human                                                      Rabbit          Dako                                               A0008         097
Alpha-Sarcomeric Actin            Rabbit                            monoclonal, 5C5          Mouse           Sigma                                              A2172                        NO
Androgen receptor (AR; N20)       Human (recombinant)                                        Rabbit          Santa Cruz                                         sc-816        A2003          Citrate (5 cycles)
Angiopoietin 3 (M18)              Mouse (recombinant)                                        Goat            Santa Cruz                                         sc-9355
Angiopoietin 3 (R19)              Mouse (recombinant)                                        Goat            Santa Cruz                                         sc-9357
Apaf1                             Human                             polyclonal               Rabbit          Cell Signaling                                     #4542         1
ARF (H50)                         Human (recombinant)                                        Rabbit          Santa Cruz                                         sc-9063       B221
B2Microglobulin                   Human                             monoclonal               Mouse           Thermo Scientific                                  MA1-83331
Bax                               Human (recombinant)                                        Rabbit          R&D Systems                                        AF820
Bax                               Human(synthetic peptide)          polyclonal               Rabbit          Cell Signaling                                     #2772         4
Bcl2                              Human (recombinant)               monoclonal, C-2          Mouse           Santa Cruz                                         sc-492        C1403
Bcl2                                                              polyclonal                 Rabbit          Cell Signaling                                     #2876         3
bcl-2                             Mouse (recombinant)                                        Goat            R&D Systems                                        AF810
Bcl-6 (N3)                        Human (recombinant)                                        Rabbit          Santa Cruz                                         sc-858        H201           EDTA (Catoretti)
Beta catenin                      Human (recombinant)             monoclonal, NA             Mouse           Chemicon                                           MAB2081                      Citrate (5 cycles)
Beta catenin                      Human (recombinant)                                        Rabbit          Chemicon                                           AB19022                      Citrate (5 cycles)
Beta-catenin                      Human/Mouse (synthetic peptide)                            Rabbit          Sigma                                              C2206                        Citrate (5 cycles)
Beta-catenin (C18)                Human (recombinant)                                        Goat            Santa Cruz                                         sc-1496       B1803          NO
B-Galactosidase                   E. coli                           polyclonal               Rabbit          Gene Tex                                           GTX26646      15104
Bgp1                              Mouse                                                      Rabbit          Dr. Beauchemin                                                                  Variable
Bgp2                              Mouse                                                      Rabbit          Dr. Beauchemin                                                                  Citrate (5 cycles)
BrdU                                                                monoclonal, Bu20a        Mouse           Dako                                               M0744         033 (Aug.96)   Trypsin/HCl/Sodium Borate
Brdu                                                                monoclonal, BU-33        Mouse           Sigma                                              BU-33         057K4859
BrdU, conjugated to KLH                                             monoclonal, BU-33        Rat             Sigma                                              B2531                        Trypsin/HCl/Sodium Borate
B-Tubulin                         Human(synthetic peptide)          polyclonal               Rabbit          Cell Signaling                                     #2146         2
BVP-1                             Bovine papilloma virus type I                              Rabbit          Dako                                               B0580                        NO
Casp3                             Human(synthetic peptide)          polyclonal               Rabbit          Cell Signaling                                     #9661         13
Casp3                             Human(synthetic peptide)          monoclonal               Rabbit          Cell Signaling                                     #9664         18
Casp8                             Human(recombinant)                polyclonal               Rabbit          Novus                                              NB100-56116    AR17-061206-01
Casp8                             Human(recombinant)                polyclonal               Rabbit          Abcam                                              ab4052
Casp9                             Human                             polyclonal               Rabbit          Novus                                              NB100-56118   AR19A-060106-D4
Caspase 3                         Human (recombinant)                                        Rabbit          R&D Systems                                        AF835
Caspase 3 (CPP32)                 Human (recombinant)               monoclonal, 4-1-18       Mouse           Chemicon                                           MAB4703      23021221
Cathepsin D                       Human                             monoclonal, C5           Mouse           Chemicon                                           MAB422       22120520
CC26 (Clara cell protein 26 kD)   Rat                               polyclonal               Rabbit          Chemicon                                                        2
                                                                                                                                                                AB9246(AB3700) 3030909       Variable
Ccl11                             Mouse(recombinant)                monoclonal               Rat             R &D                                               MAB420       AOQ024011
Ccl11                             Mouse(recombinant)                polyclonal               Goat            R &D                                                            AGT02
                                                                                                                                                                AF-420-NA AGT02
Ccl17                             Mouse(recombinant)                monoclonal               Goat            R &D                                               AF529        CXM02
Ccl22                             Mouse(recombinant)                monoclonal               Rat             R &D                                               MAB439 158113GXE01
Ccl24                             Mouse(recombinant)                monoclonal               Rat             R &D                                               MAB528 106521EPP02
Ccl27a                            Mouse(recombinant)                monoclonal               Goat            R &D                                               AF725        CZR016021
CCR3                              Mouse (synthetic peptide)                                  Rabbit          BD Biosciences                                     556882
CD106                             Mouse                             monoclonal, 429          Rat (IgG2b)     The Jackson Laboratory (Ted Duffy)                 TJL186B
CD106 (VCAM-1)                    Mouse                             monoclonal, M/K-2        Rat             Chemicon                                           CBL1300      176U0266
CD146                             Human                             monoclonal, P1H12        Mouse           Chemicon                                           MAB16985     21121094
CD19                              Mouse                             monoclonal, ID3          Rat (IgG2b)     The Jackson Laboratory (Ted Duffy)                 TJL167B
Cd19                 Mouse(recombinant)         monoclonal             Rat       BD Pharmingen                        550284 1D3      39613
CD22                 Mouse                      monoclonal, 2D6        Rat       Chemicon                             CBL1311           1311451723
CD28 (M-20)          Mouse (recombinant)                               Goat      Santa Cruz                           sc-1624         K198
Cd29                 Mouse                                             Hamster   BioLegend                            102209 102210   B128837
CD29                 Mouse                      monoclonal, OXM718     Hamster   Chemicon                             CBL1333
CD3                  Mouse                      monoclonal, 17A2       Rat       Chemicon                             CBL1302         13023893
CD3 (PC3/188A)       Human                      monoclonal, PC3/188A   Mouse     Santa Cruz                           sc020047        I1102
CD31 (PECAM-1)       Mouse                      monoclonal, MEC13.3    Rat       BD Biosciences                       550274
Cd34                 Mouse                      monoclonal             Rat       Santa Cruz                           sc-18917        I1508
CD38                 Mouse                      monoclonal, 90         Rat       Chemicon                             CBL1338         1338516143     NO
CD3-d (M-20)         Mouse (recombinant)                               Goat      Santa Cruz                           sc-1129         F010           EDTA (Catoretti)
CD3e                 Mouse                                             Rat       The Jackson Laboratory               TJL 154 P
CD3e                 Mouse                      monoclonal, 145-2C11   Hamster   The Jackson Laboratory (Ted Duffy)   TJL010B
Cd3e                 Mouse(recombinant)         monoclonal             Hamster   BD Pharmingen                                550277 15798
CD3-e                Mouse                      monoclonal, KT3        Rat       Chemicon                             CBL1317        21080415
CD3-gamma (C-18)     Mouse (recombinant)                               Goat      Santa Cruz                           sc-1125        F010            NO
CD4                  Unknown                    monoclonal, GK1.5      Rat       Chemicon                             CBL1305        130561P
CD4                  Mouse                      monoclonal, YTS191.1   Rat       The Jackson Laboratory               TJL 099P
Cd4                  Human                      polyclonal             Goat      Santa Cruz                           sc-1140        C239, B2404
Cd4                  Mouse(recombinant)         monoclonal             Rat       BD Pharmingen                                553648 03702
CD4 (C-18)           Human (recombinant)                               Goat      Santa Cruz                           sc-1140        J3002
Cd40                 Mouse                      monoclonal, 3/23       Rat       Abcam                                ab22469       196359
Cd40                 Mouse                      monoclonal             Hamster   BD Pharmingen                        553721 HM40-3
CD40 (C-20)          Human (recombinant)                               Goat      Santa Cruz                           sc-1731       J3102
Cd44                 Mouse                      monoclonal             Rat       BD Pharmingen                        550538 IM7      08175
CD45                 Mouse                      monoclonal, C363.16A   Rat       Chemicon                             CBL1343         1343227113     NO
Cd45                 Mouse(recombinant)         monoclonal             Rat       BD Pharmingen                               553097   0000051997
Cd45                 Mouse(recombinant)         monoclonal             Rat       BD Pharmingen                               553098   06709
CD45R/B220           Mouse                      monoclonal, RA3-6B2    Rat       Chemicon                             CBL1342         22010573       Variable
CD49d                Mouse                      monoclonal, R1-2       Rat       BD Biosciences                       553154
CD62L                Mouse                      monoclonal, MEL-14     Rat       Chemicon                             MAB2151Z        23030949       Citrate (5 cycles)
CD68 (H-255)         Human (recombinant)                               Rabbit    Santa Cruz                           sc-7084         G101           Citrate (5 cycles)
CD83 (FL-205)        Human (recombinant)                               Rabbit    Santa Cruz                           sc-20083        E132
CD8a                 Mouse                      monoclonal, YTS169.4   Rat       The Jackson Laboratory               TJL O98P
CD8a                 Mouse                      monoclonal, 53-6.72    Rat       The Jackson Laboratory               TJL 032 P
Cd8a                 Mouse                      monoclonal             Rat       BD Pharmingen                        550281 53-6.7   85277
cdk1/p34cdc2         Xenopus                    monoclonal, A17.1.1    Mouse     Chemicon                             MAB8878         18100662       Citrate (5 cycles)
CDK2                 Human                                             Mouse     BD Biosciences                       610145
CDK4                 Human (recombinant)        monoclonal, DCS-35     Mouse     Chemicon                             MAB8879         22110362       Citrate (5 cycles)
Cdk4 (C-22)          Mouse (recombinant)                               Rabbit    Santa Cruz                           sc-260          A1403
C-myc (9E10)         Human (recombinant)                               Rabbit    Santa Cruz                           sc-262          B2603
Collagen IV          Mouse                                             Rabbit    Chemicon                             AB756P                         Variable
Collagen IV          Human                      monoclonal, IV-4H12    Mouse     Chemicon                             MAB3326
Collagen IV          Mouse                                             Rabbit    Chemicon                             AB756                          YES
Crisp1               Human                      polyclonal             Goat      Santa Cruz                           sc-21276        C0204
Csta                 human                      polyclonal             Goat      Santa Cruz                           sc-33279        c1006
Cxcl10               Mouse(recombinant)         polyclonal             goat      R&D                                  AF-466-NA       AHC0308061
Cxcl16               Mouse(recombinant)         polyclonal             Goat      R&D                                  AF503           FMN010871
Cxcr3                Mouse(synthetic peptide)   polyclonal             Rabbit    MBL                                  EP-2049         452391 A
Cyc1                 Human(synthetic peptide)   polyclonal             Rabbit    Cell Signaling                       #4272           4
Cyclin A             Human (recombinant)                               Rabbit    Rockland                             100-401-151     2626
Cyclin A (H-432)     Human (recombinant)                               Rabbit    Santa Cruz                           sc-751          L1602          EDTA (Catoretti)
Cyclin B1            Human (recombinant)                               Rabbit    Rockland                             100-401-152     2627
Cyclin D1            Human (recombinant)                               Rabbit    Rockland                             100-401-153     11756
Cyclin D1 (72-13G)   Mouse (recombinant)        monoclonal, 72-13G     Mouse     Santa Cruz                           sc-450          G252           EDTA (Catoretti)
Cyclin D3 (C-16)     Human (recombinant)                               Rabbit    Santa Cruz                           sc-182          C1803          EDTA (Catoretti)
Cyclin E             Human (recombinant)                               Rabbit    Upstate                              06-459
cytokeratin          Human                      monoclonal             Mouse     Millipore                            IHCR2027-6      JH1555196
Cytokeratin 15       Mouse                      monoclonal, LHK15      Mouse     Chemicon                             CBL272          1508U0015
Desmin                                   Human                             monoclonal, DE-B-5    Mouse        Chemicon                                      MAB3430        22090574     Citrate (5 cycles)
DVN-1                                    Mouse                                                   Mouse        Dr. Derry Roopenian                                                       NO
Edar                                     Human                             polyclonal            Goat         Santa Cruz                                    sc-15290       I1604
EGF                                      Mouse                                                   Rabbit       Sigma                                         E2635                       NO
EGFR                                     Human (synthetic peptide)         monoclonal            Mouse        Sigma                                         E3138                       Citrate (5 cycles)
Eosinophil peroxidase                    Human                             monoclonal            Mouse        Chemicon                                      MAB1087        21080539     Citrate (5 cycles)
Eosinophilic major basic protein         Unknown                           monoclonal, AHE-2     Mouse        Chemicon                                      MAB1086        21120015
Eosinophilic major basic protein         Human                             monoclonal, BMK13     Mouse        Chemicon                                      CBL419         4192705P
Ephrin B1 (C-18)                         Human recombinant (identical to                         Rabbit       Santa Cruz                                    sc-910         A0803        Citrate (5 cycles)
ERB-4 (C-18)                             mouse ephrin B1 and B2)
                                         Human (recombinant)                                     Rabbit       Santa Cruz                                    sc-283         C0603        Citrate (5 cycles)
erbB2                                    Rat                               monoclonal, 42        Mouse        BD Biosciences                                610161
Estrogen receptor (aa120-170)            Bovine                            monoclonal, AER304    Mouse        Chemicon                                      MAB443
F4/80 (macrophages)                      Mouse                                                   Rat          The Jackson Laboratory                        TJL O46P
Fa2h                                     Human(recombinant)                polyclonal            Rabbit       Protein Tech                                  15452-1-AP
Factor VIII-related antigen              Human                                                   Mouse        Dako                                          M0616          093          should be von Willebrand factor
Fas/TNFRSF6 (CD95)                       Mouse (recombinant)                                     Goat         R&D Systems                                   AF435
Fibronectin                              Human                             monoclonal, FN-15     Mouse        Sigma                                         F7387
Filaggrin                                Mouse                                                   Rabbit       Covance                                       PRB-417P                    NO
Flg                                      Mouse                             polyclonal            Rabbit       Covance                                       PRB-417P       14830801
Flk1                                     Mouse (recombinant)               monoclonal, A3        Mouse        Santa Cruz                                    sc-6251
Follicular Dendritic Cell                Mouse                             monoclonal            Rat          BD Pharmingen                                 551320 FDC-M113904
Foxn1                                    Human(synthetic peptide)          polyclonal            Rabbit       Aviva                                         ARP30053-T200QC2528
Foxq1                                    Human(synthetic peptide)          polyclonal            Rabbit       Chemicon                                      AB4131       0512017009
Foxq1                                    Human                             polyclonal            Goat         Santa Cruz                                    sc-47597     B2007
Foxq1                                    Human(synthetic peptide)          polyclonal            Goat         Abcam                                         ab5098       352566
Foxq1                                    Human(synthetic peptide)          polyclonal            Rabbit       Abcam                                         ab51340
Gamma catenin                            Human (recombinant)               monoclonal, 11 E4     Mouse        Chemicon                                      MAB2083                     Citrate (5 cycles)
Gata3                                    Human                             polyclonal            Rabbit       Santa Cruz                                    sc-9009        E0907
GFP                                                                        polyclonal            Rabbit       Chemicon                                      AB3080         0701050861
GFP                                      Aequorea victoria                 polyclonal            Rabbit       Abcam                                         ab290          513303
Glial fibrillary acidic protein (GFAP)   Human                                                   Rabbit       Sigma                                         G9269                       Citrate (5 cycles)
Ha1                                      Human                                                   Mouse        Dr. Fleckman
Ha2                                      Human                                                   Mouse        Dr. Fleckman
Ha5                                      Human                                                   Mouse        Dr. Fleckman
Hb1                                      Human                                                   Mouse        Dr. Fleckman
Hoxc13                                                                                           guinea pig   Abcam                                         BP5116         911160
IgG4                                     Human                             monoclonal, G17-4     Mouse        BD Biosciences                                555882         30238
Il1f6                                    Mouse(recombinant)                monoclonal            Rat          R &D                                          MAB22971       XKF016061
IL7R-alpha                               Mouse                             monoclonal, A7R34     Rat          eBioscience                                   14-1271
Integrin alpha 6                         Unknown                           monoclonal, MA6       Rat          Chemicon                                      MAB1982
Integrin beta 1                          Unknown                           monoclonal, MB1.2     Rat          Chemicon                                      MAB1997                     Citrate (5 cycles)
Involucrin                               Mouse                                                   Rabbit       Covance                                       PRB-140c                    NO
IRF-4 (M-17)                             Mouse (recombinant)                                     Goat         Santa Cruz                                    sc-6059        J0802        EDTA (Catoretti)
Itgax                                    Mouse                             monoclonal            Hamster      BD Pharmingen                                          550283 65568
Itgax                                    Mouse                             monoclonal            Hamster      BD Pharmingen                                          557400 87405
JAK1                                     Human/Mouse (recombinant)                               Rabbit       Upstate                                       06-272                      Citrate (5 cycles)
JAK2                                     Mouse (recombinant)                                     Rabbit       Upstate                                       06-255                      Citrate (5 cycles)
JunB                                     Human(synthetic peptide)          polyclonal            Rabbit       Abcam                                         ab11981        336101
Junb                                     Human(synthetic peptide)          polyclonal            Rabbit       Abcam                                         ab31421
Keratin 1                                Mouse                                                   Rabbit       Covance                                       PRB-165P                    NO
Keratin 1                                Human                                                   Mouse        Dr. Fleckman
Keratin 10                               Mouse                                                   Rabbit       Covance                                       PRB-159P                    NO
Keratin 10                               Human                             monoclonal, DE-K10    Mouse        Dr. Fleckman, NeoMarkers (Labvision, Thermo   MS-611                      Citrate (5 cycles)
Keratin 13                               Human                             monoclonal, Ks 13.1   Mouse        Scientific)
                                                                                                              Chemicon                                      CBL176                      NO
Keratin 13                               Human                             monoclonal, KS-1A3    Mouse        Sigma                                         C0791
Keratin 14                               Mouse                                                   Rabbit       Covance                                       PRB-155P                    NO
Keratin 14, C-terminus                   Human (recombinant)               monoclonal, LL002     Mouse        Dr. Fleckman, NeoMarkers                      MS-115                      Citrate (5 cycles)
Keratin 15, C-terminus                   Human (recombinant)               monoclonal, LHK15     Mouse        Dr. Fleckman                                  MS-1068                     Citrate (5 cycles)
Keratin 16, C-terminus                   Human (recombinant)               monoclonal, LL025     Mouse        Dr. Fleckman                                  MS-620                      Digestion
Keratin 17                        Rat                        monoclonal             Mouse         Chemicon                            MAB1677
Keratin 17                        Rat                        monoclonal, E3         Mouse         Dr. Fleckman, NeoMarkers            MS-489
Keratin 17                        Mouse (recombinant)                               Rabbit        Dr. Pierre Coulombe                                               NO
Keratin 18                        Human                      monoclonal, Ks18.04    Mouse         Chemicon                            CBL177
Keratin 18                        Human                      monoclonal, DC10       Mouse         Chemicon                            MAB1600
Keratin 18                        Unknown                    monoclonal, RGE53      Mouse         Chemicon                            MAB3234                       Digestion
Keratin 18                        Human                      monoclonal, M9         Mouse         Monosan                             MON3006
Keratin 20                        Human                      monoclonal, Ks 20.8    Mouse         Chemicon                            CBL208
Keratin 3/12                      Rabbit                     monoclonal, AE5        Mouse         Chemicon                            CBL218        21812101p-01    Digestion
Keratin 5                         Mouse                                             Rabbit        Covance                             PRB-160P                      NO
Keratin 6                         Mouse                                             Rabbit        Covance                             PRB169P                       NO
Keratin 6, C-terminus             Human (recombinant)        monoclonal, LHK6B      Mouse         Dr. Fleckman, NeoMarkers            MS-766                        Citrate (5 cycles)
Keratin 6a                                                                          Guinea pig    Dr. Roop                            K6-212                        Citrate (5 cycles)
Keratin 6a/b                                                                        Rabbit        Dr. Roop                            K6-83                         Citrate (5 cycles)
Keratin 6b                                                                          Rabbit        Dr. Roop                            K6-82                         Citrate (5 cycles)
Keratin 7                         Human                      monoclonal, LP5K       Mouse         Chemicon                            CBL194                        NO
Keratin 7                         Human                      monoclonal, LdS68      Mouse         Chemicon                            CBL184
Keratin 7                         Unknown                    monoclonal, RCK105     Mouse         Monosan                             MON3007                       Digestion
Keratin 8                         Human                      monoclonal, LP3K       Mouse         Chemicon                            CBL195
Keratin 8                         Human                      monoclonal, NA         Mouse         Chemicon                            MAB1634
Keratin 8                         Human                      monoclonal, LP3K       Mouse         Chemicon                            CBL195
Keratin 8                                                    monoclonal, EL41       Potoroo       Dr. E.B. Lane
Keratin 8, 18                     Bovine                                            Guinea pig    Progen                              GP11                          Citrate (5 cycles)
Keratin 8/18                                                 monoclonal, EL61       Potoroo       Dr. E.B. Lane
Keratins 4, 5, 6, 8, 10, 13, 18   Human                      monoclonal, C11        Mouse         Chemicon                            CBL234                        Digestion
Keratins 4, 5, 6, 8, 10, 13, 18   Unknown                    monoclonal             Mouse         Chemicon                            MAB1636                       NO
Keratins 5, 14                    Human                      monoclonal, LH8        Mouse         Chemicon                            CBL267
Keratins 5, 6                     Human                      monoclonal, D5/16 B4   Mouse         Chemicon                            MAB1620                       Citrate (5 cycles)
Keratins 5, 8                     Human                      monoclonal             Mouse         Chemicon                            MAB3228                       NO
Keratins 5, 8                     Unknown                    monoclonal             Mouse         Chemicon                            MAB1629                       Digestion
Keratins 7, 17                    Unknown                    monoclonal             Mouse         Chemicon                            MAB1625
Keratins 8, 18                    Human                      monoclonal, NCL-5D3    Mouse         Chemicon                            CBL170                      Digestion
Keratins type I                   Unknown                    monoclonal, AE1        Mouse         Chemicon                            CBL215                      Variable
Keratins type II                  Human                      monoclonal, AE3        Mouse         Chemicon                            MAB1611                     NO
Ki67 (MKI67)                      Human                      monoclonal, B56        Mouse         BD Biosciences                      550609                      Citrate (5 cycles)
Ki-67 (MKI67)                     Human (recombinant)        monoclonal             Mouse         Invitrogen                          08-0156
Ki67 (Mki67, M-19)                Mouse (recombinant)                               Goat          Santa Cruz                          sc-7846       A3103         Citrate (5 cycles)
Laminin                           Mouse                                             Rabbit        Sigma                               L9393                       NO
Lgals7                            Mouse(recombinant)         monoclonal             Rat           R &D                                              J
                                                                                                                                      MAB1304 212923JX013101, JJX024021
Loricrin                          Mouse                                             Rabbit        Covance                             PRB-145P                    NO
L-selectin (CD62L)                Mouse                      monoclonal, MEL-14     Rat           Chemicon                            MAB2151Z      21031198
Ly6g                              Mouse                      monoclonal, RB6-8C5    Rat           BD Pharmingen                              550291 15801
M30 CytoDEATH (cytokeratin 18)    Human                      monoclonal, M30        Mouse         Roche                               12140322001
Mac-1 (CD11b)                     Mouse                      monoclonal             Rat (IgG2b)   The Jackson Laboratory, Ted Duffy   TJL063B
Major Basic Protein               Mouse                                             Rabbit        Dr. Elizabeth Lenkiewicz                          Rabbit 509    Digestion 1.5 min
                                                                                                                                                    (10/16/98)
Mapk14                            Human(synthetic peptide)   monoclonal             Rabbit        Cell Signaling                      #9215         4
Mapk8ip3                          Humam                      polyclonal             Rabbit        Cell Signaling                      #9251         14
MHC Class II (Ia)                 Mouse                      monoclonal, M5/114     Rat           The Jackson Laboratory, Ted Duffy
Mki67                             Mouse                      polyclonal             Goat          Santa Cruz                          sc-7846       J3108
Msx2                              Human(synthetic peptide)   polyclonal             Rabbit        Sigma                               M1069         086k1959
Msx2                              Mouse                      polyclonal             Rabbit        Santa Cruz                          sc-15396      C0404
NCAM (CD56)                       Chicken                                           Rabbit        Chemicon                            AB5032        23010388
NCAM (CD56)                       Mouse                                             Rat           Chemicon                            MAB310        23020285        Citrate (5 cycles)
Ncr1                              Mouse(recombinant)         polyclonal             Goat          R &D                                AF2225        KWW01
Neurofilament                     Bovine                                            Rabbit        Sigma                               N4142                         NO
Neuron-specific enolase (NSE)     Bovine                                            Rabbit        Dako                                A0587                         NO
NK                                Mouse                      monoclonal, Dx5        Rat           The Jackson Laboratory              TJL 148
NK1.1 cells                       Mouse                      monoclonal, PK136      Mouse         The Jackson Laboratory              TJL 83
NLDC 145 (dendritic cells)        Mouse                                             Rat           The Jackson Laboratory              TJL 122
NOS2                              Human (recombinant)                               Rabbit        Santa Cruz                          sc-651
ODC                                    Mouse (recombinant)         monoclonal, ODC-29    Mouse        Sigma                    O1136




Ovalbumin                              Chicken                     polyclonal            Rabbit       Millipore                AB1225        NMM1645448
p15 (M-20)                             Mouse (recombinant)                               Goat         Santa Cruz               sc-1429       D101            Variable

p16 (H-156)                            Human (recombinant)                               Rabbit       Santa Cruz               sc-1207       K281
p19 (M-167)                            Mouse (recombinant)                               Rabbit       Santa Cruz               sc-1063       E170
p21                                    Mouse (recombinant)         monoclonal, F-5       Mouse        Santa Cruz               sc-6246       B1003
p27/Kip1 (C19)                         Human recombinant)          monoclonal, C-19      Rabbit       Santa Cruz               sc-528        C1303           Citrate (5 cycles)

p53                                    Human (recombinant)         monoclonal, DO-7      Mouse        BD Biosciences           550831(554294)                Citrate (5 cycles)
p63                                    Human (recombinant)         monoclonal, 4A4       Mouse        Chemicon                 MAB4135        2212133        NO
p63                                    Human recombinant           monoclonal, 4A4       Mouse        Santa Cruz               sc-8431        L0302          EDTA (Catoretti)
Pancadherin                            Human (synthetic peptide)                         Rabbit       Sigma                    C3678                         NO
Parp1                                  Mouse(synthetic peptide)    polyclonal            Rabbit       Cell Signaling           #9544          3
Pax                                    Mouse (recombinant)                               Rabbit       Covance                  PRB-278P
PCNA                                   Mouse (recombinant)         monoclonal, PC10      Mouse        Santa Cruz               sc-56          A0903          Variable
Pdpn                                   Mouse                       monoclonal            Hamster      abcam                    ab11936        625728
PECAM 1 (M-20)                         Mouse (recombinant)                               Goat         Santa Cruz               sc-1506
PECAM-1                                Mouse                       monoclonal, 390       Rat          Chemicon                 CBL1337        133715882P
Phospho-beta-catenin (Ser33/37Thr41)   Human (synthetic)                                 Rabbit       Cell Signaling           9561           NONE
Phospho-beta-catenin (Thr41/Ser45)     Human (synthetic)                                 Rabbit       Cell Signaling           9565           NONE
Plin2                                                                                    guinea pig   ARP                      03-6P40        608100-1
Pou5f1                                 Human                       polyclonal            Rabbit       Santa Cruz               sc-9081        G0607
P-selectin                             Mouse                                             Rabbit       Chemicon                 CBL40          40161P-01      EDTA (Catoretti)
Rb (C-15)                              Human (recombinant)                               Rabbit       Santa Cruz               sc-50          B0503
Rel B                                  Mouse (recombinant)                               Rabbit       Santa Cruz               sc-226         C2803

Rela                                   Human                       polyclonal            Rabbit       Cell Signaling           #3037        4
S100                                   Bovine                      monoclonal, 15E2E2    Rabbit       Chemicon                 MAB079       22011259      Citrate (5 cycles)
S100                                   Bovine                                            Mouse        Sigma                    S2644
Shank1, Shank2, Shank3                 Human                       polyclonal            Goat         Santa Cruz               sc-23543     A0307
Sharpin                                                                                  mouse        Affinity BioReagents     PA1-61835    060626JCS1
Shh, C-terminus                        Mouse (recombinant)                               Goat         R&D Systems              AF445
Shh, N-terminus                        Mouse (recombinant)                               Goat         R&D Systems              AF464
Soat1                                  Human(synthetic peptide)    polyclonal            Rabbit       Abcam                    ab39327      269309
Soat1                                  Human(synthetic peptide)    polyclonal            Rabbit       Abcam                    ab39327      627115
Tbx15, Tbx18                           Mouse                       polyclonal            Goat         Santa Cruz               sc-17891     C092
Tbx21                                  Mouse                       polyclonal            Rabbit       Aviva                    ARP37251-T200QC7304
TGF-beta                               Human (recombinant)                               Rabbit       Sigma                    T9429                      NO
Th                                     Human(synthetic peptide)    polyclonal            Rabbit       Novus                    NB100-92002 360346
Thy1.1                                 Mouse                       monoclonal, HO-22.1   Mouse        The Jackson Laboratory   TJL51
Thy1.2                                 Mouse                       monoclonal, HO-13.4   Mouse        The Jackson Laboratory   TJL 50
Traf2                                  Human(synthetic peptide)    polyclonal            Rabbit       Abnova                   PAB0275      AR34A-103106F
Traf2                                  Human(synthetic peptide)    polyclonal            Rabbit       Abcam                    ab37118      790325
Traf6                                                              polyclonal            Rabbit       MBL                      JM-3566-100  I-9036
Trichohyalin                           Human                       monoclonal, AE15      Mouse        Dr. T.-T. Sun                                       Citrate (5 cycles)
Tropomyosin 1                          Human (recombinant)                               Rabbit       Chemicon                 AB3228                     Citrate (5 cycles)
Trp63                                  Mouse                       polyclonal            Goat         Santa Cruz               sc-25039     E0806
Twist1                                 Human(synthetic peptide)    monoclonal            Rabbit       Sigma                    T6451        086K4815
Twist1                                 Human(recombinant)          monoclonal            mouse        Abcam                    ab50887
Tyrosin hydroxylase                    Rat                         monoclonal, TH-16     Mouse        Sigma                    T2928        105H4830      NO
Ubb                                    Human(recombinant)          polyclonal            Rabbit       Abcam                    ab7780       765540
Uvomorulin                             Mouse                       monoclonal, DECMA-1   Rat          Sigma                    U3254
Vcan                                   Mouse                       polyclonal            Rabbit       Millipore                AB1032
VEGF (A-20), N-terminus                Human                       polyclonal            Rabbit       Santa Cruz               sc-152       C2003
VEGF-R2                                Human (recombinant)         monoclonal, 260.4     Mouse        Sigma                    V3003                      NO
VEGF-R2                                Mouse (recombinant)
Vim                                    Pig                         monoclonal            Mouse        Abcam                    ab8069 V9     834811
Vimentin                               Human                                             Goat         Biomeda                  V2009         9993
Vimentin                               Human                                             Goat         Chemicon                 AB1620
Vimentin                                Human (recombinant)                                     Rabbit          Chemicon                                     CBL46                       Citrate (5 cycles)


Vitamin D receptor, intestinal          Chicken                                                 Rat             Chemicon                                     MAB1360   22061355, 04-     Citrate (5 cycles)
                                                                                                                                                                       1526 is a
                                                                                                                                                                       recommended
                                                                                                                                                                       replacement for
                                                                                                                                                                       MAB1360.
α-Tubulin                               Chicken                        monoclonal               mouse           Sigma                                        T9026




This table was last updated on 07 February 2011, by Dr. Dale Begley, The Jackson Laboratory.
See also: Mikaelian I et al., "Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor." Toxicol Pathol 2004 Mar-Apr;32(2):181-91.

Disclaimer: This list is made available for information and suggested use.
Every care has been taken to ensure the accuracy of the information supplied but some inaccuracies may be present.
In particular, the catalogue numbers for antibodies and links to websites may have been changed by the manufacturer.
We do not guarantee that the antibodies and procedures listed will provide optimal results under your or any other conditions.
Other antibodies to the same antigens may label equally well or better. Differences in batches of the same antibody may
account for differences in labeling. The listing of commercial products does not imply endorsement of the product or the
manufacturer by The Jackson Laboratory. The websites listed or linked from this site are provided for information only.
                        Fixatives and antibody dilutions tested
Dilution range tested   Fekete's       Zn              Bouin's      NBF             PFA             Recommended control tissue
50-8,000                50                                                                          Hair follicles                                                            Legend
                                                                                                                                                                                       Staining too pale
                                                                                                                                                                                       Background too high
1-10                    5                                                                           MMTV-infected mammary gland                                                        No signal
1-10                    10                                                                          MMTV-infected mammary gland                                                        Good signal
1-10                    5                                                                           MMTV-infected mammary gland                                                        Not tested
100-2,000
250-1,000               250                                                                         Intestinal epithelium
250-5,000               250            250                                          250             Intestinal epithelium
10-50
6,000-40,000            15000          15000           15000        15000           15000           Smooth muscle fibers, myoepithelial cells
1,000-5,000
5,000-20,000                                                                                        Myocardium and skeletal muscles; least background with Zinc at 1:10,000
                        100            200             100          200             200             Leydig cells (testis)




10-500




25-450



25-500                  25             25                                                           Lymphoid follicles
100-2,000                                                                                           Colon
250-1,000               500            250             500          500                             Intestinal epithelium
250-5,000               250                            250          250                             Colonic epithelium
                        450            150             300          150             150             Colon, duodenum, cornea, esophagus, epidermis

2,000-10,000            10000          5,000 -10,000   5,000        5,000 -         5,000 -         Glands with ducts and digestive tract
2,000-10,000            2000                           2000         10,000          10,000
                                                                                    2000            Colon
20                      20                                                                          Epidermis, duodenum

40                      40                                                                          Epidermis, duodenum

2000                    2000                                                                        Viral papillomas




25-750                                 25                                                           Small intestine
100-400
                        1600 (citrate) 800 (citrate)         1600             800             1600 Lung (also labels a variety of other tissues)




200-1,000
10-50
25-500
100-1,000
10-50
50                           50                   50           50               50               Spleen, lymph nodes
25-500

50-1000
100-1000
25-1000
10-100

50-500                            50                                                             B cells (spleen and Peyer's patches), thymic medulla
               25                 25                                                             Paracortical areas in lymph nodes, Periarteriolar lymphoid sheaths in spleen,
20-500                                                                                           thymus (scattered cells)
10-50

100-1000
               25                                                                                Intra-epithelial T cells of duodenum
20-500
100-500



10-200



10-500

50-500         250                                     250          250              50          Intraepithelial T cells in intestine, thymocytes in thymic medulla, periarteriolar
                                                                                                 lymphoid sheaths; also works on whole gastro-intestinal mounts

25-500         50 (no AgR)        125 (with AgR), 50   100          100 (AgR)        100 (AgR)   Lymphoid tissues
100-2,000                         (w/o AgR)            (AgR)
25                           25                   25           25
               400                400                  400          200              200         Small intestine (scattered epithelial cells)
10-200
20-500
20-500

50-500
1,000-10,000
100-500
10-300
10-300
20-100                            100                                                            Kidney
50-4500
500-2,500      500                500                  500                                       Basement membranes




100-2,000
               50                 50                   25           25               25          Thymus
100-2,000
100-2,000
               100                                                                               Tumors (no labeling in non-neoplastic tissues)
25-100                            100                                                100         Thymus
100-2,000

               5                  5                    5            5                5           Haired skin
               1       1       1       1       1       Smooth muscles and striated skeletal muscles; also artifactually labels the
                                                       Labeling with many fixatives (1:100) and in frozen and mammary luminal cells
                                                       transitional epithelium, the cornea, salivary glands,tissues in wt and transgenic with
                                                       mouse
1,000-5,000    2000            1000            2000    Ducts of salivary glands, epidermis
500-5,000      500     500                             Axones of peripheral nerve
50-500         150                                     skin of cpdm mice
50-500
50-500
               100     100     50      200     100     Colon and duodenum (scattered epithelial cells)
               800     600     600     600             Nuclear labeling of the cells of the crypts of small intestine, cardiomyocytes,
1,000-5,000                                            neurones
50-500
50-500

50-1000

100-2,000
5,000-35,000   15000   10000   10000   5000    5000    Stratum corneum

100-2,000




100-2,000




100-7,500      7500                    2500            Glial cells
1-50
1-50
1-50
1-50

50-130

               100             100     100             Granulocytes
25-1,000
50-1,000               1000                            Intestine
4,000-10,000   7500                    7500    7500    Epidermis
               25              25      50



100-2,000      100             100     500             Gastric epithelium epidermis
100-2,000      100                             100     Urothelium., most epithelia



6,000-25,000   12000           6000            12000   Epidermis
1-25                                                   Haired skin
2,500-15,000   2500    2500    2500            2500    Epidermis
50-1,000       200             50              50      Haired skin
100-500        100     200     100             100     Epidermis
50-1,000
6,000-25,000   25000   12000   12000   25000   6000    Epidermis
50-500         50      100     50              50      Oral mucosa, haired skin
5-500          5       5                       5       Haired skin
1-500          1                                       Oral mucosa, haired skin
1-200
50-1,000                                                                            Cornea, epidermis, mammary gland
750-2,500      2,500         750          2,500         2,500         2,500         Hair follicles; also labels the wall of some arteries
100-2,000
100-2,000
100-2,000      500                        200                         500           Ducts and ductules in exocrine pancreas and salivary glands
10-50
50-1,000
50-500         200                        200           200           200           Haired skin
7,000-30,000   7000                       7000                        7000          Epidermis
6,000-25,000   12000         6000         6000                        6000          Epidermis
5-500                                                                               Haired skin
500-2,000      500                        500                                       Oral mucosa, haired skin
500-2,000      500           500          1000          500           500           Haired skin
250-2,000                                                                           Oral mucosa, haired skin
25                           25                                                     Urothelium
50-1,000
25-1,000       50                                                                   Outer root sheath; does not label the structures it is supposed to label (transitional
100-500                                                                             epithelium)
100-2,000
100-2,000
5
300-600        450           450          600                                       Colonic epithelium; also labels structures it is not supposed to label
5
50-1,000       50                                                                   Does not label all structures it is supposed to label
50-1,200       1000          1000         1000                                      Haired skin
25-1,000
50-200         100                        50                                        Haired skin
50-1,000       250           250          250           250                         PFA is great for the CL of the ORS; discrepencies between the fixatives
50-1,000       500           500          500                         500           Esophagus, tongue; does not label all structures it is supposed to label
50-1,750                                                                            Haired skin, oral mucosa
25-500         25                         25                                        Colonic epithelium
100-500        200           200          150                                       Haired skin
50-1,000       50            50           (AgR)
                                          50                                        Haired skin; does not labell all structures it is supposed to label
25-100         25            25                                                     Intestinal epithelium, lymphoid follicles

               200           200          200           200           200           Thymus
3,000-15,000   3000          3000                                                   Basement membrane of skeletal muscle fibers and blood vessels

4,000-15,000   8000          8000         8000          8000                        Epidermis
25-750


10-50
2000-4000      2000                                                                 cpdm mouse tissues




250                    250          250           250           250           250




               650           650          650           650           650
               100           200          50            50            50

3,500-15,000   7000                                                                 Axones of peripheral nerves, neurones
4,000-15,000   15000                      8000                                      Peripheral nerves, ganglion cells
20-500
20-500
20-500
               750           1000         1000          1000          1250          Dendritic cells in thymus (nuclear)
3,500-15,000                                                          Corneal epithelium shows mild diffuse labelling whenever available: artifact?
                                                                      Possible artifactual labeling of epithelial cells of the colon, brush border of proximal
                                                                      tubules of the kidney, etc at a dilution of 1:750 with AgR (Bouin's and Fekete's)


                100                             100    100 (citrate   Bone marrow
                                                       buffer)
50-200
50-200
                100                                                   Mammary tumors in mice transgenic for myc and wnt1
                25      25              25      25     25             Small intestine; Bouin's is the best but labeling is only moderate and there is
                                                                      background
25-100          25                                                    Some tumors
250-750         750     750             750     750    750            Transtional epithelium, male accessory sexual glands
                25      25              10      25     50             Basal layers of esophagus, epidermis, mammary gland, prostate, vagina, etc
1,000-100,000   5000    5000            5000    5000   5000           Epidermis, colon, placenta

500-1,000
                500     500 (citrate)   750     100    500            Duodenum, thymus

25-500                                                                Works on whole mounts (endothelial cells) at 1:400
25-750




                50      50              50      50     25             Spleen and bone marrow (megakaryocytes, platelets)
25-1000
                500     500             1000    1000   1000           Nucleus of some dendritic cells and M cells of Peyer's patches; slightly better with
                                                                      citrate buffer antigen retrieval

1,000-20,000                                                          Axones of peripheral nerves, cartilage, neurones
100-500




500-10,000      1000                                   1000           Epidermis

20-500
20-500




5-500           20      20                             10             Outer root sheath, adrenal medulla
100             100     100             100                           Smooth and striated muscle fibers




500-10,000      10000                   10000                         Adrenal medulla

50-2,000

25-500
200-1,000                                                             Artifactual labeling of endothelial cells in PFA-fixed tissues


20-80                                                                 Mesenchymal cells
50-1,000
         500   1000   1000   1000   750   Fibrocytes of the dermis, melanocytes of the epidermis, chondrocytes, smooth
                                          muscle fibers of large arteries, etc; this antibody also labels the outer root sheath,
                                          the duct of salivary glands, the conjunctiva, the corneal epithelium, etc
50-500   50    100    50     50     100   Intestinal epithelium

				
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