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					                                      HOUSING OF VERTEBRATE ANIMALS
                      The College of New Jersey Institutional Animal Care and Use Committee




                                          DIRECTIONS FOR USING THIS FORM
Background

The purpose of this questionnaire is to assure that adequate procedures are in place for the daily observation and care of
animals, and when species have special requirements.

The questionnaire is an attempt at making it as easy as possible for you to tell us how you maintain your animals. There
are no right answers (yet!). Feel free to provide additional information. We welcome your comments and suggestions.

Word Processing Instructions

1. This form will format your answers in a different typeface than the text of the form itself. When printed, it is much
   easier to distinguish answers and questions. On the screen, your answers will appear in color.

2. This form uses advanced Word for Windows formatting such as styles, tables and character formatting

3. This file is formatted to make your answers appear differently than the text of the form itself. To move through the
   form, use the Mouse, Arrow Keys or the scroll bar. DO NOT USE THE “ENTER KEY” TO ADD A LINE BELOW A
   QUESTION. If you do this, you will retain the formatting of the question in your answer. To add an additional
   paragraph in your answer, do use the “Enter Key” and another paragraph will begin.

4. It is recommended that you work with all “non-printing” characters showing. (Tools, Options, and on the View Tab
   under non-printing characters, select All.) When you do this, spaces between words appear as dots. Tabs appear as
   arrows and “paragraph marks” appear as a “¶”. Red dots and red paragraph marks indicate where you can enter
   information. As the name implies, non-printing characters do not appear in “Print Preview” or when you document is
   printed.
Rationale:

All investigators are required to provide for the care of all animals which they maintain on the college campus. This policy
outlines the conditions under which this is appropriate and establishes guidelines to be met by investigators so that the
IACUC can assure that animals receive proper care and that the animals do not pose a hazard or inconvenience to
people in the building in which they are kept.


    Concerns about animals outside of centralized animal facilities
1. Centralized animal facilities provide controlled environments with temperature control, light cycle control, adequate
   ventilation systems separate from those supplying “people areas”, security, proper facilities for storage of food and
   waste, pest control programs, cagewashing facilities, etc. Heating is provided during long holiday breaks. Animals
   are observed 365 days a year. Housing areas outside of animal facilities may not be able to provide all of these
   elements.
2. The IACUC must know about all animals on campus.
3. Experience shows that the quality of care provided in investigator labs can vary tremendously.
4. Animals must be observed frequently, including during weekends and holidays. Generally this means daily.
5. Animals, animal odors and allergens must not adversely affect people in the building.
6. Animal care practices must meet all standards as set forth in applicable regulations and guidelines. The IACUC must
   be able to assure such standards are met.


    Requirements for housing vertebrate animals outside centralized animal facilities
1. Standards of care must meet all applicable requirements.
2. The facility must be reasonably accessible to authorized personnel from the IACUC and outside inspectors or site
   visitors.
3. Animal use must be reported to the IACUC when requested.
4. The investigator must provide a detailed written SOP outlining the animal care practices to be used.
5. Protocol approval will be conditional on approval of the husbandry SOP.

 Return Completed Questionnaire with a copy of an animal care checklist to the Chair of the TCNJ Animal Care and Use
          Committee. This should accompany a copy of the Animal Care and Use protocol as an attachment.
                                   Send to: Dr. Andrew Leynes, leynes@tcnj.edu
                                          SPECIAL ANIMAL HUSBANDRY PROCEDURES
                                                  The College of New Jersey




    A. Project Specific Information
        1. Investigator Information
             a. Principal Investigator
             b. Protocol Number
             c.   Location of animal housing: Building:                                Room:
             d. Species: Common name (Scientific name optional):
        2. Room Information
             a. Primary room function:
                [ ] Office [ ] Lab [ ] Classroom [ ] Dedicated animal space
             b. Do other room activities generate noxious gases or vapors that could adversely affect the animals (e.g.
                insecticides, bench chemistry, etc.)?     [ ] Yes  [ ] No
                If so, explain how animals will be protected.


             c.   Room dimensions (ft)
             d. Room construction and facilities (check all applicable)
                  [   ]   Windows
                  [   ]   Floor drain
                  [   ]   Sink
                  [   ]   Electrical outlets
                  [   ]   Ground fault interruption for outlets
                  [   ]   Air conditioning
                  [   ]   Heating
                  [   ]   Individual room temperature control
                  [   ]   Heat is provided during extended vacation periods
                          Room supply:         [ ] 100% fresh air
                                               [ ] recirculated (a common situation for offices)
                          Room exhaust:        [ ] 100% exhaust
                                               [ ] recirculated (a common situation for offices)
                          Air balance:         [ ] Pressure: room positive to hallway/adjoining spaces
                                               [ ] Pressure: room negative to hallway/adjoining spaces
                                               [ ] Don’t know




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Investigator:                                                                              Animal Husbandry Procedure
Species:
Protocol No.:

        3. Personnel and emergency procedures
             a. Who will be responsible for maintenance of animal cages and observation of animals? Give name, title
                and experience or qualifications. If students are in a course, individuals need not be named, but give the
                names of the person supervising the students.


             b. Describe procedures for providing weekend and holiday care. Indicate who (e.g. regular animal care
                staff, students, part-time staff, etc.) provides and oversees care and what procedures are performed.
                Indicate qualifications of weekend/holiday staff if not regular staff.


             c.   Describe procedures for monitoring animal facility mechanical systems and notifying appropriate
                  personnel in the event of a significant failure that occurs outside regular work hours.




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Investigator:                                                                                 Animal Husbandry Procedure
Species:
Protocol No.:



    B. Animal Environment, Housing, and Management (taken from AAALAC Description information)
Not all questions will be relevant to your situation. Few protocols will need to respond to all questions. If a question is not
relevant to your protocol, enter “Not applicable” or “N/A”. A separate outline is available for aquatic species.

This outline is designed to give a person not familiar with your animal husbandry program a comprehensive picture of how
the animals are cared for. It may be viewed by the Institutional Animal Care and Use Committee.
The basis for this outline is the Guide for the Care and Use of Laboratory Animals. To view the full text of the Guide go to:
http://oacu.od.nih.gov/regs/guide/guide3.htm . Any procedures at variance with the recommendations of the Guide should
be described in your animal care and use protocol and must be approved as part of that protocol. The following outline is
taken from the Association for the Assessment and Accreditation of Laboratory Animal Care, International (AAALAC, Intl)
husbandry description for inclusion in accreditation submissions. AAALAC uses the Guide as the basis for minimum
standards.
For assistance in developing procedures and/or completing the form, contact Elizabeth Dodemaide at 732 445-4168 or
dodemaide@orsp.rutgers.edu.


        1. Physical Environment

             a. Housing
                 1) Primary Enclosures
                     a) Cage and pen environments
        Describe the cage and pen environments. Include general descriptions of composition, condition,
        flooring, special ventilation or barrier aspects, access to food and water.



                     b) Allowance for social interaction, and allowance for normal physiologic and behavioral needs
        Describe allowance for social interaction, and allowance for normal physiologic and behavioral needs.



                 2) Sheltered or Outdoor Housing
                     a) Barns, corrals, pastures, and islands
        Describe environments in areas such as barns, corrals, pastures, and islands.



                     b) Protection of animals from weather extremes, predators, and escape
        Describe methods used to protect animals from weather extremes, from predators, and from escape.



                     c) Enrichment and group housing
        Describe protective or escape mechanisms for submissive animals, provisions for enrichment, and efforts
        to group compatible animals.



                     d) Floors or ground surfaces
        Describe the floors or ground surfaces.




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Investigator:                                                                             Animal Husbandry Procedure
Species:
Protocol No.:



                     e) Provision of food and water
        Describe how food and water are provided.



                     f)   Capture
        Describe how animals are captured.



                 3) Naturalistic Environments
                     a) Description
        Describe types (forests, pastures, islands) of naturalistic environments and how animals are monitored for
        animal well-being.



                     b) Provision of food and water
        Describe how food, water, and shelter are provided.



                     c) Capture
        Describe how animals are captured.



             b. Animal Space Provisions
                 1) Determination of adequate cage or pen size or housing densities
        Describe references and considerations used to determine adequate cage or pen size or housing
        densities.



                 2) Exceptions to the Guide and applicable regulations
        Describe space exceptions to the Guide and applicable regulations.



             c. Temperature and Humidity
        Describe briefly the heating and air conditioning system performance. Provide method and
        frequency for assessing, monitoring, and documenting animal room or housing area temperature and
        humidity. Note: current detailed (by room) performance data are to be provided as indicated on the
        enclosed HVAC form. If outdoor housing areas are used, so note.



             d. Ventilation
                 1) Performance aspects of the ventilation system
        Briefly describe the performance aspects of the ventilation system. Provide method and frequency
        for assessing, monitoring, and documenting the animal room ventilation rates and pressure gradients
        (with adjacent areas). Note: current (within the last 12 months) detailed (by room) information is to be


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Investigator:                                                                              Animal Husbandry Procedure
Species:
Protocol No.:

        provided as indicated on the enclosed HVAC form.



                  2) Ventilated cages
        Describe ventilation aspects of any special primary enclosures using forced ventilation.



                  3) Recycled air
        If any air used in a room or primary enclosure is recycled, describe the source of the air and how gaseous
        and particulate contaminants are removed.



                  4) Illumination
        Describe lighting system, intensity, photoperiod, waterproof light fixtures (yes/no), automatic control
        (yes/no), windows (yes/no).



             f)   Noise
                  1) Control of excessive noise
        Describe design features and methods used to control/reduce/prevent excessive noise in the animal
        facility.



                  2) Use of background noise
        Describe any regularly used background noise (e.g., tapes, radio) system in the animal rooms.



    2. Behavioral Management
             a. Structural environment
        Describe the structural environment of primary enclosures that may enhance the well-being of
        animals housed in cages, pens, or shelters.



             b. Social environment
                  1) Enhancing physical contact and communication
        Describe efforts made to provide or enhance physical contact and communication among members
        of the same species.



                  2) Isolated or individually housed animals
        Describe steps taken with isolated or individually housed animals to compensate for the absence of
        other animals (e.g., interactions with humans, environmental enrichment).



             c. Activity


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Investigator:                                                                            Animal Husbandry Procedure
Species:
Protocol No.:

                 1) Species-typical activity
        Describe provisions to allow animals to exhibit species-typical activity patterns (e.g., walking dogs,
        access to pens, opportunity for exploration, foraging, and exercise).



                 2) Forced activities
        Describe instances of forced activities for reasons other than to meet therapeutic or approved protocol
        objectives.



    3. Husbandry
             a. Food
                 1) Type and source
        Describe type and source of food stuffs.



                 2) Storage facilities of vendors
        Describe storage facilities of vendors, noting temperature and vermin control measures. If more than
        one source, describe each.



                 3) Facilities in the animal facility
        Describe storage facilities in the animal facility noting temperature and vermin control measures. If
        more than one facility, describe each.



                 4) Food storage in animal rooms
        Describe food storage in animal rooms.



                 5) Food preparation areas
        Describe food preparation areas.



                 6) Provision of food to various species
        Describe how food is provided to various species (ad libitum, limited amounts, types of feeders).



                 7) Food quality control procedures
        Describe special food quality control procedures including procedures for rotating stock, monitoring
        milling dates, nutritional quality, bio-load, chemical contaminants, etc.



             B. Water
                 1) Source, treatment or purification


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Investigator:                                                                             Animal Husbandry Procedure
Species:
Protocol No.:

        Describe source, treatment or purification process, and how provided to the animals (pans, bottles
        with sipper tubes, automatic watering, etc.).



                 2) Quality control
        Methods of quality control including monitoring for contaminants.



             c. Bedding
                 1) Type(s), how used, and how selected
        Describe type(s), how used, and how selected for various species (direct or indirect).



                 2) Storage facilities
        Describe storage facilities including vermin control measures (if more than one, describe for each area).



                 3) Bedding quality control procedures
        Describe bedding quality control procedures including monitoring for contaminants.



             d. Miscellaneous Animal Care and Use Equipment
                 This section need not be repeated for each animal facility.
                 1) Motorized vehicles
        Describe motorized vehicles used for transporting animals noting the type and how the cargo
        compartment is climatically controlled.



                 2) Other animal care related equipment
        Described other animal care related equipment used in the animal care program (specialized
        equipment for exercise or enrichment, high pressure sprayers, vacuum cleaners, etc.).



             e. Sanitation
                 1) Bedding change
                     a) Frequency of contact and non-contact bedding changes
        Describe frequency of contact and non-contact bedding changes for each species and cage type
        (solid bottom or suspended) or pen, and if other than recommended in the Guide, the criteria used to
        determine that frequency.



                     b) Approved exceptions to frequencies recommended in the Guide or in regulations
        Describe any IACUC-approved exceptions to frequencies recommended in the Guide or in regulations.




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Investigator:                                                                              Animal Husbandry Procedure
Species:
Protocol No.:

                     c) Where soiled bedding is removed
        Note the location where soiled bedding is removed from the cages and where clean bedding is placed
        into the cages.



                 2) Cleaning and disinfection of primary enclosures
                     a) Washing/sanitizing frequency
                     b) Note the washing/sanitizing frequency for each of the following:
                          i. Solid bottom cages

                          ii. Suspended wire bottom or slotted floor cages

                          iv. Cage racks and shelves

                          v. Cage pans under suspended cages

                          vi. Play pens, floor pens, stalls, etc.


                     c) Cage and equipment washing/sanitizing procedures
        Describe the cage and equipment washing/sanitizing procedures (mechanical washers, hand
        washing, high pressure sprayers, etc.).



                     d) Cleaning/sanitizing agents
        List the cleaning/sanitizing agents (specify generic or broad category) used.



                     e) Exceptions to Guide (or other regulations)
        Describe any IACUC-approved exceptions to Guide (or other regulations) recommended sanitization
        intervals.



                 3) Cleaning and disinfection of secondary enclosures
                     a) Animal room cleaning frequency
        Describe animal room cleaning frequency, procedures (floors, walls, ceilings, ducts, exposed pipes,
        and fixtures), methods (hose, high pressure washer, vacuum cleaner, hand brushing, mopping, etc.), and
        cleaning/sanitizing agent(s) (generic name) used.



                     b) Corridor and support area cleaning
        Describe the corridor and support area cleaning frequency and procedures and cleaning/sanitizing
        agents (generic name) used.



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Investigator:                                                                             Animal Husbandry Procedure
Species:
Protocol No.:



                     c) Procedures for sanitizing cleaning implements
        Describe the procedures for sanitizing cleaning implements such as mops and mop buckets.



                     d) Cleaning implements that are shared among animal rooms
        Note those cleaning implements that are shared among animal rooms and those that are provided for
        each room and not moved between animal rooms.



                  4) Sanitation of Cage Equipment
                     a) Sanitation procedures and frequency for feeders
        Describe sanitation procedures and frequency for feeders including those used with animal
        enhancement/exercise programs and portable cages as well as pens, runs, stalls, etc.



                     b) Sanitation procedures and frequency for watering devices
        Describe sanitation procedures and frequency for watering devices such as bottles, sipper tubes,
        bowls, automatic watering systems (distribution lines, connection coils, rack manifolds, valves), etc.



                     c) Sanitation procedures and frequency for exercise devices, manipulanda used in environmental
                        enrichment program, etc
        Describe sanitation procedures and frequency for exercise devices, manipulanda used in environmental
        enrichment program, etc.



                  5) Sanitation of Transport Cages, Equipment, and Vehicles
        Describe frequency and procedures for sanitizing transport cages, equipment, and vehicles used for
        transporting animals, clean cages, feed and bedding, etc.



                  6) Assessing the effectiveness of sanitation
        Describe how the effectiveness of sanitation procedures is monitored (water temperature monitoring,
        microbiologic monitoring, visual inspections, etc.).



             f.   Waste Disposal
        Describe the handling, storage, method and frequency of disposal, and final disposal location for each of
        the following:



                  1) Soiled bedding and refuse


                  2) Animal carcasses



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Investigator:                                                                               Animal Husbandry Procedure
Species:
Protocol No.:



                 3) Hazardous wastes-infectious, toxic, radioactive


             g. Pest Control


                 1) Program for controlling pests
        Describe the program for controlling pests (insects, rodents, etc.) noting the control agent(s) used,
        where applied, and who oversees the program and applies the agent(s).



                 2) How animal users are informed


             h. Emergency, Weekend, and Holiday Care


                 1) Weekend and holiday care


                 2) Contacting responsible animal care and/or veterinary personnel
        Describe procedures for contacting responsible animal care and/or veterinary personnel in case of an
        emergency.



                 3) Monitoring animal facility mechanical systems


                 4) Responding to a disaster
        Briefly describe the plan for responding to a disaster and identify those institutional components which
        would participate in the response.



    4. Population Management
             a. Identification and Records
                 1) Animal identification methods
        Describe animal identification methods for each species (e.g., cage cards, tattoo, neck chains, etc.).
        Indicate information contained on cage cards.



                 2) Procedure(s) for maintaining individual records
        Describe procedure(s) for maintaining individual records on animals. Identify the species for which
        individual records are maintained, individuals (titles, not necessarily names) responsible for maintaining
        the records, and where they are maintained and how veterinary and IACUC access is assured.



             b. Genetics and Nomenclature



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Investigator:                                                                                     Animal Husbandry Procedure
Species:
Protocol No.:

                 1) Describe program and advising investigators
        Describe program and advising investigators on selection of animals based on genetic characteristics.



                 2) Using standardized nomenclature
        Describe program for advising investigators on using standardized nomenclature to ensure proper
        reporting of the identification of the research animals with regard to both the strain and substrain or the
        genetic background of all animals used in a study.



                 3) If breeding colonies are maintained:
                      a) Species, strains and stocks
        Note the species, stock or strain, and the number of generations they have been separated from parent
        line.



                      b) Breeding schemes
        Describe the breeding schemes used to ensure homozygosity of inbred strains or heterogeneity of
        outbred stocks and to minimize genetic drift.



                      c) Genetic monitoring
        Describe the genetic monitoring program to ensure homozygosity and genetic uniformity and continuity of
        inbred animals.



                      d) Substrain designation
        Note if a identifying the genetic background of the animals is used routinely when reporting the results of
        experiments involving animals from in-house breeding colonies.




A daily record of animal husbandry procedures should be maintained. This is best done with a form that records animal care for a
week or a month a time. Items to include will depend on the species and cage design, but might include daily observation, feeding or
checking feed, watering or checking water, maximum and minimum temperature in the room for the day, and a record of cage
sanitation, floor mopping, etc. The form should be initialed to indicate who did the work. Forms should be maintained for review. A
sample form is included as an example.
                      Please attach a copy of the form to be used to record animal husbandry procedures.


 Return Completed Questionnaire with a copy of an animal care checklist to the Chair of the TCNJ Animal Care and Use
          Committee. This should accompany a copy of the Animal Care and Use protocol as an attachment.




6e601a6d-ce5c-44ed-ab07-e1868cc5f7a3.doc                      10/25/11                                                   Page 11
                                                               RECORD OF ANIMAL CARE PROCEDURES

Animal Care Tech:                PI:               Facility:                              Room No.:
Veterinarian (732 445-4168):     Prot.No.                                                 Species:
Feb-01                  TH FR SA SU MO TU WE TH FR SA SU MO TU WE TH FR SA SU MO TU WE TH FR SA SU MO TU WE TH FR SA
Daily                    1   2 3  4    5  6 7 8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Ch food/water
Ch bedding
Ch/Rpt health Prob
Flush auto/water
Record temp.
Ch/Rpt Rm Prob
Sweep floors
Sch. Cleaning Days
Exchange bedding
Exchange bottles
Fill feeders
Mop floors
Check Soap&Towels
Other:
Weekly
Ch/Cl air filters
Clean sinks
    doors
Dust as needed
Clean mop bkts
Ch./Adj timers
Other:
Bi-weekly
Exchange bar lids
Exch hang cages
Clean/flush drains
Monthly
Exch shelf racks
Clean barrels
Clean walls
Other:
TECH'S INITIALS
VERIFY CENSUS
Animals must be observed at least daily, including weekends and holidays. Exceptions to this rule must be part of the approved animal use protocol.


6e601a6d-ce5c-44ed-ab07-e1868cc5f7a3.doc                      10/25/11                                                     Page 1

				
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