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The study of burn healing of Onosma stenosiphon on type II burn of back and
testis areas in rats
Mohammad Ali Khalili 1, Seyad Mohsen Miresmaeili 2, Hosain Hekmati Moghaddam3 ,
Shahnaz Rezaei4, Ali Reza Vahidi 5
1- Associate Professor, Department of Anatomy, Yazd University of Medical Sciences, Yazd, Iran
2- ACECR Higher Education Institute, Yazd, Iran (Corresponding author: smm230@gmail.com)
3- Research and Treatment for Infertility Center , Yazd University of Medical Sciences, Yazd, Iran
4- Assistant Professor, Department of Pathology Yazd University of Medical Sciences, Yazd, Iran
5- Assistant Professor, Department of Pharmacology, Yazd University of Medical Sciences, Yazd, Iran
ARTICLE INFO ABSTRACT
Type: Research Original Background & Aim: Onosma stenosiphon has been used as herbal
Topic: Pharmacology, medicine for healing skin cuts and rashes. However, burn healing
effect of this herb has not been yet reported in pharmaceutical texts.
Ethnopharmacology
The aim of this study was to find out the effect of herb ointment on
Received 1 January 2010 burn healing the superficial wounds post type II burns of back and
Accepted 7 February 2010 testis region in Wistar rats.
Experimental: A total of 24 rats were divided into 3 (21 rats) and 1
Key words:
control (3 rats) groups. Type II burn was performed on right and left
Onosma stenosiphon Boiss. sides of back and right testicular skin of all experimental animals. For
Burn healing burn healing, herb ointment was applied on right sides of back and
Testis testis skins of experimental rats. For histo-pathological evaluation,
seven rats were killed at 7, 14, 21 days intervals. Then, skin samples
Rats
were obtained from both left and right (with herb ointment) sides of
back, as well as testicular tissue samples. All samples from
experimental animals were compared with control samples following
tissue staining.
Results & Discussion: All skin samples (both with and without herb
ointment applications) showed patho-morphological changes one
week post experimental burns. Two week following burns, epidermis
were relatively healed, and lymphohistocytological inflammation
with initiation of fibrosis was observed. But, epidermis and dermis
inflammation were recovered after three weeks. There were no
noticeable changes in healing rate of burned tissues in rats under herb
ointment versus non ointment applications. Also, there were no
alternations in testicular tissue or spermatozoa cells in experimental
groups.