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BIOGRAPHICAL SKETCH (PAN, DIPANJAN)



NAME: Dipanjan Pan POSITION TITLE:

eRA COMMONS USER NAME: DIPANJANPAN Research Assistant Professor of Medicine

Washington University in St. Louis



Education/training

INSTITUTION AND LOCATION (MENTORS) DEGREE YEAR FIELD

Vidyasagar University, India MS 1997 Organic Chemistry

Indian Institute of Technology, Kharagpur, India PhD 2002 Synthetic Chemistry



Washington University in St. Louis (Department of Chemistry) Postdoc 2002-05 Polymer Chemistry,

Nanotechnology



B. Positions and Honors

PERIOD TITLE INSTITUTION

1998-2000 Junior Research Scholar Indian Institute of Technology, Kharagpur, India

2000-2002 Senior Research Scholar Indian Institute of Technology, Kharagpur, India

2002-2005 Postdoctoral Research Associate Washington University in Saint Louis, MO



2005-2006 IP Analyst General Electric Global Research, Biosciences, Bangalore



2006-2006 Diploma on Patent and litigation Won, Central Board of GO, Hague, NL



2006-2007 Postdoctoral Research Associate Washington University in Saint Louis, MO

08/07–06/10 Research Instructor of Medicine Division of Cardiology, Washington Univ School of Medicine

07/10- Research Assistant Professor of Division of Cardiology, Washington Univ School of Medicine

Medicine



Selected Honors and Fellowship Awards

2010- MO-Life Pilot project grant (St. Louis Institute of Nanomedicine), 2010-2011

2010- Best paper award, St. Louis Institute of Nanomedicine, 2010

2010- Editorial board member, J of Biotechnology and Biomaterials

2009- Scientific Advisory Board Member, Lifeboat Foundation

2009- Editorial board member, World Journal of Radiology

2008- National Scientists Development Grant (American Heart Association) 2008-2012

2008- Reviewer /Referee for journals from Institute of Physics, American Chemical Society, Royal

Society of Chemistry, Wiley Interscience

2008- Elected member, Sigma XI, The scientific research society

2008- Associate Research Member, Siteman Cancer Center for Nanotechnology Excellence; WUSM

2006- Corporate Awards: Management Award: GE Global Research, JFWTC

2001-02 CSIR, India senior research fellowship

1998-01 Indian Institute of Technology Junior Research Fellowship









US Issued and Pending Patents

1. Becker ML, Fang H, Li X, Pan D, Rossin R, Sun X, Taylor JS, Turner JL, Welch MJ, Wooley KL. Cell

permeable nanoconjugates of shell-crosslinked knedel (SCK) and peptide nucleic acids ("PNAs") with

uniquely expressed or over-expressed mRNA targeting sequences for early diagnosis and therapy of

cancer. Washington University. (2006) US Pat No. 2006159619.

2. Lanza GM, Wickline, SA, Pan D, “Water Soluble Nano-Bialys: A Vascularly Constrained, Slow

Releasing Nano-carrier for Therapeutic Imaging” (2007) US provisional 60/978,679, 10-9-07.

WO2009049083.

3. Lanza GM, Wickline, SA, Pan D, “Particles for Imaging (Hard Particle Filing)” (2007) US provisional

60/980,955, 10-19-07. WO 2009049089

4. Lanza GM, Wickline, SA, Pan D, “Particles for Imaging (Soft Particle Filing)” (2007) US provisional

60/981,192, 10-19-07. WO 2009049089

5. Lanza GM, Wickline, SA, Senpan A, Pan D, “Development of colloidal iron oxide contrast agent for

magnetic resonance imaging (MRI) and magnetic particle imaging (MPI)” (2007) US provisional

60/978,678, 10-9-07. WO 2009049089



C. 15 Recent peer-reviewed publications (selected from 35 peer-reviewed journal articles)

1. Pan D, Roessl E, Scholmka J, Caruthers SD, Senpan A, Scott MJ, Allen JS, Zhang H, Hu G, Gaffney PJ,

Choi ET, Rasche V, Wickline SA, Proksa R, Lanza GM. Computed Tomography in Color: NanoK-

enhanced Spectral CT Molecular Imaging. Angew. Chem. Int. Ed. 2010 (in press)

2. Pan D, Caruthers SD, Senpan A, Schiemeder AH, Wickline SA, Lanza GM: Revisiting an old friend:

manganese-based MRI contrast agents. Nanomedicine and Nanobiotechnology, 2010 (in press).

3. Pan D, Caruthers SD, Chen J, Winter PM, Senpan A, Schiemeder AH, Wickline SA, Lanza GM:

Nanomedicine strategies for molecular targets with MR and optical imaging. Future Med. Chem. March

2010, 2(3), 471-490.

4. Pan D, Pramanik M, Senpan A, Ghosh S, Wickline SA, Wang LV, Lanza GM: Near infrared photoacoustic

detection of sentinel lymph nodes with gold nanobeacons. Biomaterials 2010, 31, 4088-4093.

5. Pan D, Williams TA, Senpan A, Allen JS, Scott MJ, Gaffney PJ Wickline SA, Lanza GM: Detecting

Vascular Biosignatures with a Colloidal, Radio-Opaque Polymeric Nanoparticle. J. Am. Chem. Soc., 2009;

131(42), 15522–15527.

6. Senpan A, Caruthers SD, Rhee I, Mauro NA, Pan D, Hu G, Scott MJ, Fuhrhop RW, Gaffney PJ, Wickline

SA, Lanza GM. Conquering the dark side: colloidal iron oxide nanoparticles. ACS Nano. 2009;3(12), 3917-

26.

7. Pan D, Pramanik M, Senpan A, Yang X, Song KH, Scott MJ, Zhang H, Gaffney PJ, Wickline SA, Wang

LV, Lanza GM: Molecular Photoacoustic Tomography with Colloidal Nanobeacons. Angew. Chem. Int. Ed.,

2009, 121 (23), 4243-4237.

8. Pan D, Senpan A, Caruthers SD, Williams T, Scott MJ, Gaffney PJ, Wickline SA, Lanza, GM: Sensitive

and efficient detection of thrombus with fibrin-specific manganese nanocolloids. Chemical

Communications. 2009, 3234-3236.

9. Pan D, Lanza, GM Wickline SA Caruthers SD: Nanomedicine: Perspective and promises with ligand-

directed molecular imaging. Eur. J. Radiol. 2009; 70(2), 274-285.

10. Pan D, Caruthers SD, Hu G, Senpan A, Scott MJ, Gaffney PJ, Wickline SA, Lanza, GM: Ligand-directed

Nanobialys as Theranostic Agent for Drug Delivery and Manganese based Magnetic Resonance Imaging

(MRI) of Vascular Targets. J. Am. Chem. Soc., 2008; 130 (29): 9186–9187.

11. Rossin R, Pan D, Qi K, Turner JL, Welch MJ, Wooley KL: 64Cu-labeled folate-conjugated shell cross-

linked nanoparticles for tumor imaging and radiotherapy: synthesis, radio labeling and biological

evaluation. J Nucl. Med. 2005; 46:1210.

12. Turner JL, Pan D, Plummer R, Chen Z, Whittaker AK, Wooley KL: Synthesis of Gadolinium Labeled Shell

Crosslinked Nanoparticle for Magnetic Resonance Imaging Applications. Adv. Func. Mat. 2005; 15:1248.

13. Pan D, Turner JL, Wooley KL: Shell crosslinked nanoparticles designed to target angiogenic blood

vessels via avß3 receptor-ligand interactions. Macromolecules 2004; 37:7109.

14. Pan D, Turner JL, Wooley KL: Folic acid conjugated nanostructured materials designed for cancer cell

targeting. Chemical Communications 2003; 19:2400.

15. Pan D, Mal SK, Kar GK, Ray JK: Highly chemoselective method for the synthesis of 4-hydroxy-4-

allylcyclohexa-2,5-dienone derivatives by indium-mediated allylation protocol. Tetrahedron 2002; 58:2847-

2852.



D. RESEARCH SUPPORT



0835426N (Pan) 7/1/2008-6/30/2012 4.2

American Heart Association $70,000 calendar

A Nanomedicine Approach to Coronary Ruptured Plaque with spectral Computed Tomography



The aims of the proposal are 1) Synthesis and characterization of thrombus (fribin) specific

nanoparticles for K-edge-based Spectral CT imaging. Synthesize and physic-chemically

characterize novel bismuth based nano-colloids (BiNCs) for Spectral CT and optimize fibrin-bound

BiNCs for spectral CT contrast using in vitro clot phantoms. Determine the stability of the Spectral

CT BiNCs to sterilization, exposure to blood/plasma, and storage. 2) Evaluate and refine Spectral

CT bismuth nano-colloids (BiNCs) in vivo for detection of intravascular thrombus. Demonstrate

fibrin-specific Spectral CT agent for detection and quantification of fibrin specific BiNCs in dogs

(first in situ and then circulating). Delineate the pharmacokinetics, bio-distribution and gross

toxicological behavior of the preferred fibrin-targeted Spectral CT BiNCs in dogs.



U54 CA119342 (Wickline) 9/30/2005-8/31/2010 6

National Institutes of Health $369,333 calendar

The Siteman Center of Cancer Nanotechnology Excellence at Washington University

Project 1 - Neovascular-Directed Nanoparticles for Detection, Characterization and Treatment of

Neoplasia with MRI (Lanza)

The subject of this proposal is the application of a novel paramagnetic site-targeted contrast

"platform technology" for sensitive and specific imaging of molecular epitopes expressed on tumor

neovasculature alone and in combination with the local delivery of chemotherapeutic agents to

these sites.



091076 (Pan) 3/1/2010-2/28/2011 1.56

Missouri Life Sciences Research Board $35,000 calendar

Development and characterization of K-edge metal nanocolloids (NanoK) for detection of thrombus with

spectral computed tomography (Spectral CT)

The specific aims of this project are:

(A) Synthesize and characterize Spectral CT K-edge metal nanocolloids

(NanoK) and define their stability with sterilization and storage. (1-5 months)

For the preliminary demonstration of in vitro, ex vivo and in vivo spectral CT contrast, fibrin specific bismuth

nanocolloids were used. The K-edge x-ray absorption of bismuth is very high (~90KeV) in comparison to Au

(~80KeV) and W (~60KeV), which often becomes problematic in terms of separating Bi from photo effect.

Therefore, for this project, while we continue to explore the bismuth nanoparticles, we will emphasize on the

development of gold and tungsten based NanoKs. More specifically, we will synthesize and characterize novel

K-edge metal based nanocolloids (NanoK-Au, W). The goal would also be to chemically and physically

characterize NanoK and define the stability with sterilization and storage. The gross toxicological behavior of

NanoK will be determined in vitro.

(B) Characterize NanoK in vitro and vivo for detection of intravascular

thrombus with spectral CT and delineate in vivo preliminary clearance and toxicity profile. (5-12 months)

Anti-fibrin NanoK of varying compositions emerging as candidates from aim 1 will be studied first in vitro to

characterize their spectral and CT signals followed by their in vivo evaluation. More specifically, NanoKs will be

characterized in vivo for detection of intravascular thrombus with Spectral CT in a rabbit model and in vivo

preliminary clearance and toxicity profile will be delineated.

# not assigned (Pan) 1/1/2011-12/31/2012 2.4

American Heart Association $68,130 calendar

Photoacoustic tomography theranostic approach to diagnosis and treatment of carotid disease



This proposal will develop nanomedicine techniques and advanced noninvasive photoacoustic

imaging technologies to achieve more sensitive diagnosis of unstable/ruptured plaque to help

stratify patients into appropriate treatment regimens, and to stabilize or reverse luminal

thrombosis and plaque progression processes.







Completed Research Support

N01-CO-27103 Karen L Wooley (PI) 3/1/2002-6/30/2004

National Cancer Institute

Department of Chemistry at Washington University

Nanoscale Bioconjugates as Passive and Active Detection, Diagnosis and Treatment Systems

The subject of this proposal was the development of polymeric nanoparticle based multi-component

recognition units that will harness the information generated from the human genome project to prepare

devices that can be produced with a few building blocks by an automated process for cancer-specific mRNA

targeting and imaging, and thus, early detection and diagnosis.

Role: Researcher



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