Colilert Lab Bench Sheet
Collection Lab Prep Incubation Read Results
Total
coliform E coli
Yellow Yellow+
Only Fluoresce Total Coliform E coli
# Large Wells
# Large Wells
# Small Wells
# Small Wells
MPN/100
Sterile mL=
Site Name water MPN Table MPN MPN/100mL
(Indicate Blank or Dupe as Sample vol Dilution Start from MPN X from = Table MPN
Site # needed) Date Time Vol (mL) (mL) Factor Date Time Date Time Table Dilution Table X Dilution
1
2
3
4
5
6
7
8
9
10
11
12
Notes:
1. At least one lab blank and lab duplicate sample should be performed for every 10 samples run simultaneously (i.e., run 1 blank and dupe per batch)
2. Samples should be read at 24 hours
3. Count the number of cells with color change and fluorescence and use table to determine MPN
4. Multiply table reading by dilution factor as needed to determine final MPN value
Colilert Lab Bench Sheet
5. If running more than one dilution, use results of least diluted sample
Incubator Temperature Log
Staff Date / Time Temp (Deg C) Comments
Colilert Lab Bench Sheet
Notes:
1. Record temperature at least when samples for a given batch are first placed in incubator and when samples are read
2. Incubator temperature for E Coli samples by Colilert is 35 deg C
Project
Collection Lab Prep Incubation
Sterile
Site Name water
(Indicate Blank or Dupe as Sample vol Dilution Start
Site # needed) Date Time Vol (mL) (mL) Factor Date Time
1 100 #DIV/0!
2 100 #DIV/0!
3 100 #DIV/0!
4 100 #DIV/0!
5 100 #DIV/0!
6 100 #DIV/0!
7 100 #DIV/0!
8 100 #DIV/0!
9 100 #DIV/0!
10 100 #DIV/0!
11 100 #DIV/0!
12 100 #DIV/0!
13 100 #DIV/0!
14 100 #DIV/0!
Incubator Temperature Log
Staff Date / Time Temp (Deg C) Comments
Notes:
1. Record temperature at least when samples for a given batch are first placed in incubator and when samples are read
2. Incubator temperature for E Coli samples by Colilert is 35 deg C
Read Results
Total
coliform E coli
Yellow Yellow+
Only Fluoresce Total Coliform E coli
# Large Wells
# Large Wells
# Small Wells
# Small Wells
MPN/100
mL=
Table MPN MPN/100mL
MPN from MPN X from = Table MPN
Date Time Table Dilution Table X Dilution
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
#DIV/0! #DIV/0!
ts
when samples are read