Colilert Lab Bench Sheet
Collection
Lab Prep
Incubation
Read
Total coliform Yellow Only E coli Yellow+ Fluoresce
Results
Total Coliform
E coli
# Large Wells
# Large Wells
# Small Wells
# Small Wells
Site #
Site Name (Indicate Blank or Dupe as needed)
Date
Time
Sample Vol (mL)
Sterile water vol (mL)
Dilution Factor
Start Date
Time
Date
Time
MPN from Table
MPN/100 mL= Table MPN X Dilution
MPN from Table
MPN/100mL = Table MPN X Dilution
1 2 3 4 5 6 7 8 9 10 11 12 Notes: 1. At least one lab blank and lab duplicate sample should be performed for every 10 samples run simultaneously (i.e., run 1 blank and dupe per batch) 2. Samples should be read at 24 hours 3. Count the number of cells with color change and fluorescence and use table to determine MPN 4. Multiply table reading by dilution factor as needed to determine final MPN value
Colilert Lab Bench Sheet
5. If running more than one dilution, use results of least diluted sample
Incubator Temperature Log Staff Date / Time Temp (Deg C) Comments
Colilert Lab Bench Sheet
Notes: 1. Record temperature at least when samples for a given batch are first placed in incubator and when samples are read 2. Incubator temperature for E Coli samples by Colilert is 35 deg C
Project Collection Lab Prep Incubation
Site #
Site Name (Indicate Blank or Dupe as needed)
Date
Time
Sample Vol (mL)
Sterile water vol (mL)
Dilution Factor
Start Date
Time
1 2 3 4 5 6 7 8 9 10 11 12 13 14
100 100 100 100 100 100 100 100 100 100 100 100 100 100
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Incubator Temperature Log Staff Date / Time Temp (Deg C) Comments
Notes: 1. Record temperature at least when samples for a given batch are first placed in incubator and when samples are read 2. Incubator temperature for E Coli samples by Colilert is 35 deg C
Read
Total coliform Yellow Only E coli Yellow+ Fluoresce
Results
Total Coliform
E coli
# Large Wells
# Large Wells
# Small Wells
# Small Wells
Date
Time
MPN/100 mL= Table MPN from MPN X Table Dilution
MPN from Table
MPN/100mL = Table MPN X Dilution
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ts
when samples are read