Posters
Purine and pyrimidine analogues
P01 M. Bjerke: Alterations of mitochondrial DNA in CEM cells selected for resistance
towards ddC toxicity
P02 F. Bontemps: Study of the efficacy of a pronucleotide of 2-Chloro-2’-deoxy-
adenosine in Deoxycytidine-kinase deficient lymphoma cells
P03 S. Lisart: Inhibition of the ERK pathway promotes apoptosis induced by 2-chloro-2’-
deoxyadenosine in the B-cell leukemia cell line EHEB
P04 F. Bontemps: Apoptosis induced by 2-chloro-2’-deoxyadenosine is associated with
cell cycle activation in the B-cell leukemia cell line EHEB
P05 B. Krawczyk: Alteration of DNA methylation status in K562 and MCF-7 cancer cell
lines by nucleoside analogues
P06 N. Matulová: Acyclic nucleoside phosphonates and human telomerase inhibition
P07 Y. Rubin: Physico-chemical properties of azasubstituted nucleic acid base analogs
P08 M. Česnek: Guanidino Purines and Their Biological Activity
P09 O.H. Temmink: Trifluorothymidine resistance induced in H630 human colon cancer
cells is associated with decreased thymidine kinase (TK) expression
P10 A. Kotalczyk: Uptake and metabolism of 6- mercaptopurine (6-MP) and
6-thioguanine (6-TG) by different human and animal cell lines
P11 K. Fotoohi: The role of concentrative nucleoside transporter, CNT3 for resistance to
6-mercaptopurine and 6-thioguanine in T-lymphoblastic cell lines
Enzymes
P12 E. Marinello: Adenosine kinase from rat liver: new biochemical properties
P13 B. Cerboni: PARP-1 activity in systemic lupus erythematosus and other autoimmune
diseases
P14 P. Augoustides-Savvopoulou: Purine nucleoside phosphorylase deficiency
masquerading as orbital cellulitis
P15 J. Sigmond: Enhanced activity of deoxycytidine kinase and thymidine kinase 1 and 2
after pulsed low dose rate and single dose gamma-irradiation
P16 T. Spasokoukotskaja: Opposite effects of intracellular calcium chelation on the
activities of cytosolic deoxynucleoside kinases
P17 B. Munch-Petersen: Effect of C-terminal of human cytosolic thymidine kinase
(TK1) on in vitro stability and enzymatic properties
P18 D.-N. Chaperon: Complementation of the essential Escherichia coli thymidylate
kinase gene tmk by bacteriophage, fungal, bacterial, and human homologues:
Sensitivity of transgenic bacteria towards nucleoside analogues
P19 N. Solaroli: Substrate specificity of three viral thymidine kinases (TK); vaccinia
virus TK, feline herpesvirus TK and canine herpesvirus TK
P20 A. Calascibetta: Difference in Ki67 and thymidylate synthase expression in
primary tumour compared with metastatic nodes in breast cancer patients
P21 F. Escartin: In vivo study of thymidylate synthesis in Helicobacter pylori
P22 V. Nemeth: Structural and functional characterization of the nucleocapsid-dUTPase
P23 V. Domkin: Purification and crystallization of an alpha2beta2 complex of
recombinant aerobic T4 phage ribonucleotide reductase
P24 A. Fyrberg: Evidence of cell cycle-dependent regulation of Deoxycytidine kinase and
Cytosolic 5´-nucloetidase-I activity
P25 P. L. Ipata: Metabolomics of Purine and Pyrimidine Salvage Pathways. Key role
of IMP-GMP Specific Cytosolic 5’-Nucleotidase, Uridine Phosphorylase, and Uridine
Kinase in the Cross Regulation of Purine- and Pyrimidine-Nucleotide Salvage
Synthesis
P26 C. Barsotti: Evidence for the Involvement of Cytosolic 5’-Nucleotidase in the
Synthesis of Guanine Nucleotides from Xanthosine
P27 G. Spoto: cAMP phosphodiesterase activity evaluation in human carcinoma of
salivary glands
P28 R. Pesi: Ecto 5’-nucleotidase is altered in a neurological syndrome
P29 R. Sadej: Studies on the catalytic and adhesive function of ecto-5´-nucleotidase
(eN,CD73) in human melanoma cells
P30 S. De Vos: Engineered nucleoside hydrolases as prodrug-activating enzymes in cancer
therapy
P31 K. Walldén: Crystal structures of the human mitochondrial deoxyribonucleotidase in
complex with nucleoside monophosphates: Implications for the structure-aided design
of novel nucleoside analogues
P32 S. Cricca: The role of pyrimidine phosphorylases in fluoropyrimidine sensitivity of
colon cancer cells
P33 M. Inada: Pharmacokinetic consideration of [2-13C]uracil in normal and
DPD-deficient dogs for human
P34 A.B.P. van Kuilenburg: Activity of pyrimidine degradation enzymes in normal
tissues
P35 M. Löffler: Dihydroorotate Dehydrogenase from Eukaryotes: Protein Characterization
and Structure Analysis
P36 M. Löffler: Evaluation of uridine metabolism in human spermatozoa
P37 R.T. Smolenski: AMP deaminase and AMP-regulated protein kinase interaction in the
heart cells
P38 A. H.Y. Yuen: Differences in the Activities of the of Enzymes of Nucleotide
Metabolism and its Implications for Cardiac Xenotransplantation
P39 L. Osman: Stimulatory Effects of Atorvastatin on Extracellular Nucleotide
Degradation in Human Endothelial Cells
Analytical techniques
P40 J. Bierau: Determination of ITPase activity in erythrocyte lysates obtained for
analysis of TPMT activity
P41 S. Haglund: Inosine-5-monophosphate dehydrogenase activity, other enzymes and
thiopurine metabolites in patients with inflammatory bowel disease (IBD)
P42 K. Kalsi: HPLC / Tandem Ion Trap mass detector methods for determination of
inosine monophosphate dehydrogenase (IMPDH) and thiopurine
methyltransferase(TPMT)
P43 A.B.P. van Kuilenburg: Analysis of pyrimidine synthesis de novo intermediates in
urine and dried urine filter paper strips with HPLC-electrospray tandem mass
spectrometry
P44 A.B.P. van Kuilenburg: Determination of 5-fluorouracil in plasma with HPLC-
tandem mass spectrometry
P45 A.B.P. van Kuilenburg: Determination of thymidine phosphorylase activity by a non-
radiochemical assay using reversed-phase high-performance liquid chromatography
P46 S. Varadkar: A Novel High Performance Liquid Chromatography Tandem Mass
Spectrometry Method to Quantify Metabolites of the Kynurenine Pathway in
Cerebrospinal fluid (CSF)
Inborn errors
P47 H.A. Simmonds: Familial juvenile hyperuricaemic nephrophathy (FJHN) is not a rare
purine metabolic disease in Britain
P48 B. Stibůrková: Analysis of excretion fraction of uric acid
P49 J.G. Puig: The cognitive and behavioral phenotype of patients with hypoxanthine
guanine phosphoribosyltransferase deficiency
P50 R.J. Torres: Exercise-induced dystonia in a child with partial deficiency of
hypoxanthine guanine phosphoribosyltransferase
P51 J.G. Puig: Allopurinol efficacy and safety in hypoxanthine-phosphoribosyltransferase
(HPRT) deficiency
P52 I. Rychlík: An unusual cause of renal amyloidosis secondary to gout – the first
description of familiar occurrence
P53 Y. Moriwaki: Effect of beer ingestion on plasma concentrations and urinary excretion
of purine bases - one month study
P54 T. Yamamoto: Identification of a new point mutation in the human molybdenum
cofactor sulferase gene that is responsible for xanthinuria type II
P55 K. Ichida: A Turkish case with Molybdenum Cofactor Deficiency
P56 A.B.P. van Kuilenburg: A neonate with recurrent vomiting having deficiency of
dihydropyrimidine dehydrogenase
P57 A.B.P. van Kuilenburg: A pivotal role for ß-aminoisobutyric acid in
dihydropyrimidine dehydrogenase deficiency
P58 M. Zikánová: Preparation of 5-amino-4-imidazole-N-succinocarboxamideribotide,
5-amino-4-imidazole-N-succinocarboxamideriboside and succinyladenosine,
compounds usable in diagnosis and research of adenylosuccinate lyase deficiency
P59 T. Adam: High-performance liquid chromatographic assay for amidoimidazole
ribosides
P60 D. Friedecký: Models of inherited disorders of purine metabolism - nucleotide
analysis by capillary electrophoresis
P61 T. Adam: Capillary electrophoretic systems for detection of defects in purine de
novo synthesis
P62 T. Adam: Screening of purine de novo synthesis defects by TLC
P63 Z. Tsutsumi: Metabolic syndrome and gout
Genetic aspects
P64 E. Marinello: Purine metabolism in B-cell lymphocytic leukemia: A microarray
approach
P65 A. Marinaki: The ITPA 94C>A mutation (PRO32 to THR) polymorphism results in
mRNA miss-splicing
P66 M. Arenas: Genetic variation in the MTHFR gene influences TPMT enzyme activity
P67 M. Arenas: Diagnosis of molybdenum cofactor deficiency
P68 L.E. Laróvere: Hypoxanthine phosphoribosyltransferase deficiency (HPRT-d):
biochemical and molecular findings in five Argentine families
P69 G. Cuccu: DMDYT, MOTIF I: A short sequence marker deteerminating cN-II
phosphotransferase activity
P70 M. Franco: Downregulation of deoxyguanosine kinase by siRNA
P71 M. Hosoyamada: The expression level of mouse urate transporter 1 (URAT1) was
regulated by sex hormones
Adenosine + Various topics
P72 E. Zoref-Shani: The adenosine-activated neuroprotective signal transduction pathway
includes activation of phospholipase C
P73 L.G. Darlington: Adenosine and cytokine levels following treatment of rheumatoid
arthritis with dipyridamole
P74 S. Hajizadeh: The role of adenosine system on cerebral blood flow regulation
P75 P. Saiko: Cytotoxic and biochemical effects of 3,3´,4,4´,5,5´-hexahydroxystilbene, a
novel resveratrol analogue, in HL-60 human promyelocytic leukemia cells
P76 Z. Horvath: Synergistic action of resveratrol, an ingredient of wine, with Ara-C and
tiazofurin in HL-60 human promyelocytic leukemia cells
P77 K. Fabianowska-Majewska: Studies of the methylation status of CpG sequences
located in promoters of the selected tumor suppressor genes in breast cancer cells
P78 C. Felici: Purine bases in advanced carotid artery plaque
P79 E.M. Slominska: 4-Pyridone-3-carboxamide-β-D-riboside triphosphate – a new
metabolite in the human erythrocytes
P80 P. Adamski: The effect of erythrocyte ATP pool on hypoxia induced nucleotide
release