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Biobanking Reflections of the Past_ Glimpses of the Future

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Australasian perspective

Anna deFazio

National and

international

Multi-site biobank

biobanks networks and

consortia

Departmental

/ Institutional

biospecimen

banks

Biospecimen

collections







1980 1990 2000 2010

Institutional / Departmental collections

 Clinical Trials

◦ eg. Ludwig Institute, Sydney Branch

 Frozen tissues (eg. Ludwig Breast Trials)

 Plasma – pharmacokinetics (clinical trials)







 Pathology Archives

1983: 2151 citations

 Frozen tissue collections





1983









1986

 Increase in the number of Departmental / Institutional

collections and consolidation of dedicated ‘banks’

specifically established to collect biospecimens, available

for research

eg.

1992 Gyn Oncology Tissue Bank, Westmead

1997 The Kathleen Cuningham Foundation Consortium for Research into

Familial Breast Cancer (kConFab)

1998 Peter Mac Tissue Bank

1998 Children’s Hospital Westmead, Paediatric Tumour Bank

 The last decade has seen an increase in funding for biospecimen

banking in general



 Oncology: Filling the gaps in tumour types, enabling established banks

to increase the quantity and quality of their collections and increase the

provision of biospecimens for research

eg.

◦ 2002 Australian Ovarian Cancer Study

◦ 2002 NSW Pancreatic Cancer Network Tissue Bank

◦ 2002 National Leukaemia and Lymphoma Tissue Bank

◦ 2002 Singapore Tissue Network (STN)

◦ 2003 Australasian Biospecimen Network

◦ 2004 Australasian Biospecimen Network:Oncology

◦ 2005 Australian Prostate Cancer Collaboration (APCC)

◦ 2006 Breast Cancer Tissue Bank (BCTB)

◦ 2006 Victorian Cancer Biobank

◦ 2007 Sydney Melanoma Unit Bio-Specimen Bank

 The last decade has seen an increase in funding for biospecimen

banking in general



 Oncology: Filling the gaps in tumour types, enabling established banks

to increase their quantity and quality of their collections and increase

the provision of biospecimens for research.

eg.

◦ 2002 Australian Ovarian Cancer Study*

◦ 2002 NSW Pancreatic Cancer Network Tissue Bank

◦ 2002 National Leukaemia and Lymphoma Tissue Bank*

◦ 2002 Singapore Tissue Network (STN)

◦ 2003 Australasian Biospecimen Network

◦ 2004 Australasian Biospecimen Network:Oncology*

◦ 2005 Australian Prostate Cancer Collaboration (APCC)*

◦ 2006 Breast Cancer Tissue Bank (BCTB)*

◦ 2006 Victorian Cancer Biobank

◦ 2007 Sydney Melanoma Unit Bio-Specimen Bank





*NH&MRC Enabling Grants

 ‘The scheme supports excellent facilities and/or

activities where there is a clearly demonstrated

national need for the particular facilities and/or

activities in order to underpin aspects of the

national health and medical research effort’

 Over 20 special facilities related to biospecimens

banking funded including:

◦ National Network of Brain Banks

◦ Australian Motor Neuron Disease DNA Bank

◦ Australian Schizophrenia Research Bank

◦ Australian Childhood Diabetes DNA Repository

◦ The Western Australian DNA Bank

◦ Genetic Repositories Australia - a central national facility for

establishing, distributing and maintaining the long-term

secure storage of human genetic samples.

The ABN is a non-profit scientific organisation with voluntary

membership in Australia and New Zealand



 PURPOSE: to provide a forum to address technical, legal / ethical,

and managerial issues relevant to human biospecimen repositories

within Australia and New Zealand.

Statement of Purpose

 To provide an opportunity for individuals who have an interest in

tissue banking and biorepositories to come together to share

relevant and non-confidential information to assist in improving their

operational efficiencies

 To provide education and technical assistance to individuals new to

the area of biorepositories

 To provide a structure and focus around which the Australasian

Biospecimen Network Association will continue to expand and

attract a diverse range of members within the broad biobanking

community of Australia

ABN-Oncology

D. Catchpoole, J. Creaney, A. deFazio,

L. Devereux, A. Matusan, C.

Schmidt, H. Thorne, N. Zeps







 Australian Mesothelioma Tissue Bank

 kConFab

 Peter MacCallum Cancer Centre Tissue Bank as part of Victorian Cancer

Biobank

 Queensland Institute of Medical Research Cell Line Bank

 Queensland Institute of Medical Research Tissue Bank

 The Tumour Bank at Children’s Hospital at Westmead

 West Australian Research Tissue Network at Saint John of God Hospital

 Westmead Gynaecological Oncology Tissue Bank





Local, national and international linkages

ABN-Oncology

ABN Member Banks Scientific

Biospecimen and data collections Advisory

Management Executive Board









Researchers

Tissue and data requests

ABN Hub Manager

Assists researchers and tissue banks

Disseminates tissue banking information

Manages the ABN website

ABN Web site

Tissue Locator

Protocols

‘Help Desk’



www.abrn.net ‘New’ ABN Affiliated Banks

8000

Total Tissue Collection Events







7000



6000 Actual

5000



4000



3000

Estimated

2000



1000



0

Nov 04 Nov 05 Nov 06 Nov 07 Nov-08 Nov-09



Month

1400

Tissue Collection









1200



1000



800



600



400



200



0









Tumour Type

 ABN-oncology member banks have supplied

biospecimens / data to over 130 projects

 resulting in 138 publications over the last 5 years









Tumour profiles







Life-style, diet,

Inherited

environment

genes

Clinical outcome

Expression profiles

Risk Factors

Genomic gain / loss

Mutations

Genes Environment Lifestyle / Diet

Epigenetics









SNPs

Mutations



Treatment



Linkage of biospecimens with

high quality patient data

 Cancer Australia – working with ABN and other tissue banks to develop

recommended clinical dataset, with common data definitions, for

biospecimen banks.



 CheReL - The Centre for Health Record Linkage established in 2006 to

create and maintain a record linkage system for health and human

services in NSW and the ACT.



 BioGrid Australia and the Australian Cancer Grid (ACG) - a virtual

repository which will link to all participating teaching hospitals and

cancer research centres in Australia as well as the integrated cancer

centres in Victoria.



 The Australian Ovarian Cancer Study experience

Control recruitment Patient recruitment





Lifestyle and dietary

Blood Tumour sample Clinical details

questionnaires



Epidemiology Biospecimen Clinical Follow-up

Core Core Core

Penny Webb David Bowtell Anna deFazio







Recruitment 2002 - 2006

• 1,859 women with ovarian, fallopian tube and primary peritoneal cancer recruited through

treatment centres & cancer registries across Australia (1,097 frozen tissues)

• 1,066 control women recruited, selected at random from Commonwealth electoral roll

AOCS Centres

Mater Misericordiae

Royal Women’s

Townsville

Wesley









Hunter

Royal Hospital for Women

Royal North Shore / Private

King Edward Memorial Royal Prince Alfred

St John of God Westmead









Freemasons

Mercy Women’s

Burnside War Memorial

Royal Hobart Monash Medical

Flinders Medical Centre

Centre

Royal Adelaide Hospital

Royal Women’s

L







S E N S T

I I

V E

6 0 1 3

H is o

t lo g y :

S e r o u s a d e n

1 0 0 F 0 I 0

G O 0 S t

a g e C

:

I ( S a

t g e D e a

t

5









R e s id u a lD is e a s e :

m a c r

R E P S r P im O a N r S y E T r e a m

t e n :

C

t

S e c o n d L i

n e :

R e s p o n s

2









1 0 0 T 0 h 0 i

r d L n

i e N

: o R e s p o n s

F o u r h

t L i

n e :

R e s p o n s e

Serum CA125 level

1









1 0 0 0

U p p

A









C A 1

C a r

1 0 0 P a c

C









D o x

G e

S u r

1 0 R e l

a









1

F M e A Nb u Fa o- Mg ey Av - N b- u - Fa o - Mg e y Av - N b - u - Fa o - Mg e y Av - Nb - u - Fa o- Mg e y Av - Nb - u - Fa o - Mg e y v - b- - a - y

0 0 3 0 3 0 3 0 3 0 4 0 4 0 4 0 4 0 5 0 5 0 5 0 5 0 6 0 6 0 6 0 6 0 7 0 7 0 7 0 7 0 8 8

D a t e









• Clinical follow-up at 6 month intervals (GCP guidelines)

• Date of relapse assigned according to GCIG criteria (CA125)

• Treatment response assigned according to GCIG criteria

AOCS Centres Bundaberg

Cairns

Gold Coast

Hervey Bay

Mackay

Mater Misericordiae

Maryborough

Royal Women’s

Rockhampton

Townsville

Sunshine Coast

Wesley

Toowoomba

Townsville





Hunter Armidale

Royal Hospital for Women Bathurst

Royal North Shore / Private Canberra

King Edward Memorial Royal Prince Alfred Coffs

St John of God Westmead Harbour

Dubbo

Grafton

Bunbury Goulburn

Mandura Illawarra

Freemasons

Mercy Women’s Lismore

Burnside War Memorial Moruya

Royal Hobart Monash Medical

Flinders Medical Centre Orange

Centre Austin

Royal Adelaide Hospital Tamworth

Royal Women’s Bendigo Tweed Heads

Echuca

Frankston

Launceston Geelong

Whyalla Hamilton

Darwin Mildura

Mornington

Taralgon

Wodonga

October 2009

• 1,882 eligible cases

• 14,338 case report forms

• 746 (39%) eligible cases deceased

• 474 patients have completed 5 years

follow up

• 89 (4.7%) patients lost to follow-up









RN

completes

CRFs





CFU Queries /

Manager forwards





CFU

Database

Residual disease nil or ≤1 cm (n=414) Residual disease >1 cm (n=122)









Median Progression-free Survival

GG 18.3 months Large cohorts

Biospecimens

GA/GT 26.6 months

Detailed treatment details

TT/TA 38.5 months

Accurate clinical outcome data



*Johnatty SE et al. Clin Cancer Res 14: 5594–601, 2008

 Network biobanks to facilitate the collection biospecimens for clinical trials



 ABN-Oncology has already provided support for 34 national and

international clinical trials - supporting the collection of biospecimens for

trials including TARGiT, IBIS, IMPACT, GOG199



 ABN-Oncology working with COSA (Clinical Oncology Society of Australia)

to map out mechanisms / pathways to facilitate collection of biospecimens

via biobank networks

National and

Multi-site international

biobanks biobank

networks and

Departmental consortia

• Consolidation of biobank networks

/ Institutional

• Efficient linkage with patient /

biospecimen

banks

participant data

Biospecimen

• Informatics: web-based access to

collections

de-identified research data linked

to biospecimens

1980 1990 2000 2010

 ABN-Oncology: Dan Catchpoole, Jenette Creaney, Lisa Devereux,

Anita Matusan, Chris Schmidt, Heather Thorne, Nik Zeps



 Australian Ovarian Cancer Study: David Bowtell, Adele Green,

Georgia Chenevix-Trench, Penny Webb, Dorota Gertig, Nadia

Traficante, Sian Fereday, Laura Galletta, Jillian Hung, research

nurses, clinicians



 Funding: NHMRC, State Cancer Councils, Cancer Australia, US

Department of Defence

 Collection protocols

◦ A guide for developing tissue banks

◦ Consent, Tissue, Blood, Cell Lines, QA, Access Policies

◦ Completed October 2005, reviewed 6 monthly

◦ Available at www.abrn.net/protocols.htm





 Clinical Trials: Enable collection of material for clinical trials, using

existing infrastructure, on a contract basis.

 Web-based ‘Tissue Locator’: Allows researcher to search for

currently available specimens on line



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